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Q12634

- T4HR_MAGO7

UniProt

Q12634 - T4HR_MAGO7

Protein

Tetrahydroxynaphthalene reductase

Gene

MGG_02252

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of 1,3,6,8-tetrahydroxynaphthalene (T4HN) into (+)-scytalone and 1,3,8-trihydroxynaphthalene into (-)-vermelone. This enzyme is the biochemical target of several commercially important fungicides which are used to prevent blast disease in rice plants.

    Catalytic activityi

    Scytalone + NADP+ = 1,3,6,8-tetrahydroxynaphthalene + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei164 – 1641Substrate
    Active sitei178 – 1781Proton acceptor

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 6325NADP2 PublicationsAdd
    BLAST

    GO - Molecular functioni

    1. tetrahydroxynaphthalene reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. melanin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Melanin biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00785.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tetrahydroxynaphthalene reductase (EC:1.1.1.252)
    Alternative name(s):
    T4HN reductase
    Short name:
    THNR
    Gene namesi
    ORF Names:MGG_02252
    OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
    Taxonomic identifieri242507 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
    ProteomesiUP000009058: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 283282Tetrahydroxynaphthalene reductasePRO_0000054782Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    STRINGi148305.Q12634.

    Structurei

    Secondary structure

    1
    283
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 143
    Helixi22 – 254
    Beta strandi31 – 344
    Turni35 – 384
    Helixi40 – 5112
    Beta strandi55 – 628
    Helixi64 – 7613
    Beta strandi81 – 855
    Helixi91 – 10515
    Beta strandi110 – 1134
    Helixi123 – 1253
    Helixi128 – 13811
    Helixi140 – 15213
    Beta strandi158 – 1625
    Helixi165 – 1673
    Helixi176 – 19621
    Helixi197 – 1993
    Beta strandi202 – 2087
    Beta strandi211 – 2133
    Helixi214 – 2196
    Helixi220 – 2234
    Helixi232 – 24211
    Helixi252 – 26312
    Helixi265 – 2673
    Beta strandi274 – 2785

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DOHX-ray2.10A/B1-283[»]
    1G0NX-ray2.00A/B1-283[»]
    1G0OX-ray1.70A/B/C/D1-283[»]
    1YBVX-ray2.80A/B1-283[»]
    ProteinModelPortaliQ12634.
    SMRiQ12634. Positions 3-283.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12634.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK17739.
    OrthoDBiEOG75J0ZQ.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12634-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAVTQPRGE SKYDAIPGPL GPQSASLEGK VALVTGAGRG IGREMAMELG    50
    RRGCKVIVNY ANSTESAEEV VAAIKKNGSD AACVKANVGV VEDIVRMFEE 100
    AVKIFGKLDI VCSNSGVVSF GHVKDVTPEE FDRVFTINTR GQFFVAREAY 150
    KHLEIGGRLI LMGSITGQAK AVPKHAVYSG SKGAIETFAR CMAIDMADKK 200
    ITVNVVAPGG IKTDMYHAVC REYIPNGENL SNEEVDEYAA SAWSPLHRVG 250
    LPIDIARVVC FLASNDGGWV TGKVIGIDGG ACM 283
    Length:283
    Mass (Da):30,054
    Last modified:January 23, 2007 - v2
    Checksum:iE079638A96DF19CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22309 mRNA. Translation: AAA19514.1.
    AY846878 Genomic DNA. Translation: AAW55623.1.
    CM001231 Genomic DNA. Translation: EHA56411.1.
    PIRiS41412.
    RefSeqiXP_003709023.1. XM_003708975.1.

    Genome annotation databases

    EnsemblFungiiMGG_02252T0; MGG_02252T0; MGG_02252.
    GeneIDi2681349.
    KEGGimgr:MGG_02252.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22309 mRNA. Translation: AAA19514.1 .
    AY846878 Genomic DNA. Translation: AAW55623.1 .
    CM001231 Genomic DNA. Translation: EHA56411.1 .
    PIRi S41412.
    RefSeqi XP_003709023.1. XM_003708975.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DOH X-ray 2.10 A/B 1-283 [» ]
    1G0N X-ray 2.00 A/B 1-283 [» ]
    1G0O X-ray 1.70 A/B/C/D 1-283 [» ]
    1YBV X-ray 2.80 A/B 1-283 [» ]
    ProteinModelPortali Q12634.
    SMRi Q12634. Positions 3-283.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 148305.Q12634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii MGG_02252T0 ; MGG_02252T0 ; MGG_02252 .
    GeneIDi 2681349.
    KEGGi mgr:MGG_02252.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K17739.
    OrthoDBi EOG75J0ZQ.

    Enzyme and pathway databases

    UniPathwayi UPA00785 .

    Miscellaneous databases

    EvolutionaryTracei Q12634.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Polyhydroxynaphthalene reductase involved in melanin biosynthesis in Magnaporthe grisea. Purification, cDNA cloning and sequencing."
      Vidal-Cros A., Viviani F., Labesse G., Boccara M., Gaudry M.
      Eur. J. Biochem. 219:985-992(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
      Strain: Guyane 11.
    2. "Isolation, characterization and preliminary genetic analysis of laboratory tricyclazole-resistant mutants of the rice blast fungus, Magnaporthe grisea."
      Zhang C.-Q., Zhu G.N., Ma Z.H., Zhou M.-G.
      J. Phytopathol. 154:392-397(2006)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
    4. "Crystal structure of the ternary complex of 1,3,8-trihydroxynaphthalene reductase from Magnaporthe grisea with NADPH and an active-site inhibitor."
      Andersson A., Jordan D.B., Schneider G., Lindqvist Y.
      Structure 4:1161-1170(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NADP AND INHIBITOR.
      Strain: 4091-5-8.
    5. "Structures of trihydroxynaphthalene reductase-fungicide complexes: implications for structure-based design and catalysis."
      Liao D.-I., Basarab G.S., Gatenby A.A., Valent B., Jordan D.B.
      Structure 9:19-27(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH NADP AND FUNGICIDE.

    Entry informationi

    Entry nameiT4HR_MAGO7
    AccessioniPrimary (citable) accession number: Q12634
    Secondary accession number(s): A4R3G6, G4MQ18, Q5I7G0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3