Reviewed, UniProtKB/Swiss-Prot Q12634 (T4HR_MAGGR)

Names and origin

Protein names

Recommended name:
    Tetrahydroxynaphthalene reductase
    EC=1.1.1.252
Alternative name(s):
    T4HN reductase
      Short name=THNR

Gene names

ORF Names:

MGG_02252

Organism

Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) [Complete proteome]

Taxonomic identifier

148305 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 1,3,6,8-tetrahydroxynaphthalene (T4HN) into (+)-scytalone and 1,3,8-trihydroxynaphthalene into (-)-vermelone. This enzyme is the biochemical target of several commercially important fungicides which are used to prevent blast disease in rice plants.
Catalytic activity	Scytalone + NADP+ = 1,3,6,8-tetrahydroxynaphthalene + NADPH.
Pathway	Pigment biosynthesis; melanin biosynthesis.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Melanin biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	melanin biosynthetic process from tyrosine Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro tetrahydroxynaphthalene reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 283

282

Tetrahydroxynaphthalene reductase

PRO_0000054782

Regions

Nucleotide binding

39 – 63

25

NADP

Sites

Active site

178

1

Proton acceptor

Binding site

164

1

Substrate

Secondary structure

1

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283

Helix Strand Turn

Details...

Helix

12 – 14

3

Helix

21 – 25

5

Beta strand

31 – 34

4

Turn

35 – 38

4

Helix

40 – 51

12

Beta strand

55 – 62

8

Helix

64 – 76

13

Beta strand

81 – 85

5

Helix

91 – 105

15

Beta strand

108 – 113

6

Helix

123 – 125

3

Helix

128 – 138

11

Helix

140 – 152

13

Beta strand

153 – 162

10

Helix

165 – 167

3

Helix

176 – 195

20

Helix

196 – 199

4

Beta strand

202 – 208

7

Helix

214 – 219

6

Helix

220 – 223

4

Helix

232 – 242

11

Helix

252 – 263

12

Helix

265 – 267

3

Beta strand

274 – 280

7

Sequences

	Sequence		Length	Mass (Da)
	Q12634-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: E079638A96DF19CA	FASTA	283	30,054
	10 20 30 40 50 60 MPAVTQPRGE SKYDAIPGPL GPQSASLEGK VALVTGAGRG IGREMAMELG RRGCKVIVNY 70 80 90 100 110 120 ANSTESAEEV VAAIKKNGSD AACVKANVGV VEDIVRMFEE AVKIFGKLDI VCSNSGVVSF 130 140 150 160 170 180 GHVKDVTPEE FDRVFTINTR GQFFVAREAY KHLEIGGRLI LMGSITGQAK AVPKHAVYSG 190 200 210 220 230 240 SKGAIETFAR CMAIDMADKK ITVNVVAPGG IKTDMYHAVC REYIPNGENL SNEEVDEYAA 250 260 270 280 SAWSPLHRVG LPIDIARVVC FLASNDGGWV TGKVIGIDGG ACM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Polyhydroxynaphthalene reductase involved in melanin biosynthesis in Magnaporthe grisea. Purification, cDNA cloning and sequencing." Vidal-Cros A., Viviani F., Labesse G., Boccara M., Gaudry M. Eur. J. Biochem. 219:985-992(1994) [PubMed: 8112349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION. Strain: Guyane 11.
[2]	"Isolation, characterization and preliminary genetic analysis of laboratory tricyclazole-resistant mutants of the rice blast fungus, Magnaporthe grisea." Zhang C.-Q., Zhu G.N., Ma Z.H., Zhou M.-G. J. Phytopathol. 154:392-397(2006) [Agricola: IND43821857] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / FGSC 8958.
[4]	"Crystal structure of the ternary complex of 1,3,8-trihydroxynaphthalene reductase from Magnaporthe grisea with NADPH and an active-site inhibitor." Andersson A., Jordan D.B., Schneider G., Lindqvist Y. Structure 4:1161-1170(1996) [PubMed: 8939741] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NADP AND INHIBITOR. Strain: 4091-5-8.
[5]	"Structures of trihydroxynaphthalene reductase-fungicide complexes: implications for structure-based design and catalysis." Liao D.-I., Basarab G.S., Gatenby A.A., Valent B., Jordan D.B. Structure 9:19-27(2001) [PubMed: 11342131] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH NADP AND FUNGICIDE.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L22309 mRNA. Translation: AAA19514.1.
AY846878 Genomic DNA. Translation: AAW55623.1.
CH476830 Genomic DNA. Translation: EDJ98831.1.

PIR

S41412.

RefSeq

XP_365550.1.

UniGene

Mgr.5801

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DOH	X-ray	2.10	A/B	1-283	[»]
1G0N	X-ray	2.00	A/B	1-283	[»]
1G0O	X-ray	1.70	A/B/C/D	1-283	[»]
1YBV	X-ray	2.80	A/B	3-283	[»]

ModBase

Search...

Genome annotation databases

GeneID

2681349.

KEGG

mgr:MGG_02252.

NMPDR

fig|242507.1.peg.7289.

Phylogenomic databases

OMA

Q12634. DIARVVC.

Enzyme and pathway databases

BRENDA

1.1.1.252. 5953.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

T4HR_MAGGR

Accession

Primary (citable) accession number: Q12634
Secondary accession number(s): A4R3G6, Q5I7G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)