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Q12634

- T4HR_MAGO7

UniProt

Q12634 - T4HR_MAGO7

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Protein
Tetrahydroxynaphthalene reductase
Gene
MGG_02252
Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,3,6,8-tetrahydroxynaphthalene (T4HN) into (+)-scytalone and 1,3,8-trihydroxynaphthalene into (-)-vermelone. This enzyme is the biochemical target of several commercially important fungicides which are used to prevent blast disease in rice plants.

Catalytic activityi

Scytalone + NADP+ = 1,3,6,8-tetrahydroxynaphthalene + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641Substrate
Active sitei178 – 1781Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 6325NADP
Add
BLAST

GO - Molecular functioni

  1. tetrahydroxynaphthalene reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. melanin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00785.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetrahydroxynaphthalene reductase (EC:1.1.1.252)
Alternative name(s):
T4HN reductase
Short name:
THNR
Gene namesi
ORF Names:MGG_02252
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
ProteomesiUP000009058: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 283282Tetrahydroxynaphthalene reductase
PRO_0000054782Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi148305.Q12634.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 143
Helixi22 – 254
Beta strandi31 – 344
Turni35 – 384
Helixi40 – 5112
Beta strandi55 – 628
Helixi64 – 7613
Beta strandi81 – 855
Helixi91 – 10515
Beta strandi110 – 1134
Helixi123 – 1253
Helixi128 – 13811
Helixi140 – 15213
Beta strandi158 – 1625
Helixi165 – 1673
Helixi176 – 19621
Helixi197 – 1993
Beta strandi202 – 2087
Beta strandi211 – 2133
Helixi214 – 2196
Helixi220 – 2234
Helixi232 – 24211
Helixi252 – 26312
Helixi265 – 2673
Beta strandi274 – 2785

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DOHX-ray2.10A/B1-283[»]
1G0NX-ray2.00A/B1-283[»]
1G0OX-ray1.70A/B/C/D1-283[»]
1YBVX-ray2.80A/B1-283[»]
ProteinModelPortaliQ12634.
SMRiQ12634. Positions 3-283.

Miscellaneous databases

EvolutionaryTraceiQ12634.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
KOiK17739.
OrthoDBiEOG75J0ZQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12634-1 [UniParc]FASTAAdd to Basket

« Hide

MPAVTQPRGE SKYDAIPGPL GPQSASLEGK VALVTGAGRG IGREMAMELG    50
RRGCKVIVNY ANSTESAEEV VAAIKKNGSD AACVKANVGV VEDIVRMFEE 100
AVKIFGKLDI VCSNSGVVSF GHVKDVTPEE FDRVFTINTR GQFFVAREAY 150
KHLEIGGRLI LMGSITGQAK AVPKHAVYSG SKGAIETFAR CMAIDMADKK 200
ITVNVVAPGG IKTDMYHAVC REYIPNGENL SNEEVDEYAA SAWSPLHRVG 250
LPIDIARVVC FLASNDGGWV TGKVIGIDGG ACM 283
Length:283
Mass (Da):30,054
Last modified:January 23, 2007 - v2
Checksum:iE079638A96DF19CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22309 mRNA. Translation: AAA19514.1.
AY846878 Genomic DNA. Translation: AAW55623.1.
CM001231 Genomic DNA. Translation: EHA56411.1.
PIRiS41412.
RefSeqiXP_003709023.1. XM_003708975.1.

Genome annotation databases

EnsemblFungiiMGG_02252T0; MGG_02252T0; MGG_02252.
GeneIDi2681349.
KEGGimgr:MGG_02252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22309 mRNA. Translation: AAA19514.1 .
AY846878 Genomic DNA. Translation: AAW55623.1 .
CM001231 Genomic DNA. Translation: EHA56411.1 .
PIRi S41412.
RefSeqi XP_003709023.1. XM_003708975.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DOH X-ray 2.10 A/B 1-283 [» ]
1G0N X-ray 2.00 A/B 1-283 [» ]
1G0O X-ray 1.70 A/B/C/D 1-283 [» ]
1YBV X-ray 2.80 A/B 1-283 [» ]
ProteinModelPortali Q12634.
SMRi Q12634. Positions 3-283.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 148305.Q12634.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii MGG_02252T0 ; MGG_02252T0 ; MGG_02252 .
GeneIDi 2681349.
KEGGi mgr:MGG_02252.

Phylogenomic databases

eggNOGi COG1028.
KOi K17739.
OrthoDBi EOG75J0ZQ.

Enzyme and pathway databases

UniPathwayi UPA00785 .

Miscellaneous databases

EvolutionaryTracei Q12634.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Polyhydroxynaphthalene reductase involved in melanin biosynthesis in Magnaporthe grisea. Purification, cDNA cloning and sequencing."
    Vidal-Cros A., Viviani F., Labesse G., Boccara M., Gaudry M.
    Eur. J. Biochem. 219:985-992(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Strain: Guyane 11.
  2. "Isolation, characterization and preliminary genetic analysis of laboratory tricyclazole-resistant mutants of the rice blast fungus, Magnaporthe grisea."
    Zhang C.-Q., Zhu G.N., Ma Z.H., Zhou M.-G.
    J. Phytopathol. 154:392-397(2006)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.
  4. "Crystal structure of the ternary complex of 1,3,8-trihydroxynaphthalene reductase from Magnaporthe grisea with NADPH and an active-site inhibitor."
    Andersson A., Jordan D.B., Schneider G., Lindqvist Y.
    Structure 4:1161-1170(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH NADP AND INHIBITOR.
    Strain: 4091-5-8.
  5. "Structures of trihydroxynaphthalene reductase-fungicide complexes: implications for structure-based design and catalysis."
    Liao D.-I., Basarab G.S., Gatenby A.A., Valent B., Jordan D.B.
    Structure 9:19-27(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH NADP AND FUNGICIDE.

Entry informationi

Entry nameiT4HR_MAGO7
AccessioniPrimary (citable) accession number: Q12634
Secondary accession number(s): A4R3G6, G4MQ18, Q5I7G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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