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Protein

Tetrahydroxynaphthalene reductase

Gene

MGG_02252

Organism
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,3,6,8-tetrahydroxynaphthalene (T4HN) into (+)-scytalone and 1,3,8-trihydroxynaphthalene into (-)-vermelone. This enzyme is the biochemical target of several commercially important fungicides which are used to prevent blast disease in rice plants.

Catalytic activityi

Scytalone + NADP+ = 1,3,6,8-tetrahydroxynaphthalene + NADPH.

Pathwayi: melanin biosynthesis

This protein is involved in the pathway melanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway melanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164Substrate1
Active sitei178Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 63NADP2 PublicationsAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00785.

Names & Taxonomyi

Protein namesi
Recommended name:
Tetrahydroxynaphthalene reductase (EC:1.1.1.252)
Alternative name(s):
T4HN reductase
Short name:
THNR
Gene namesi
ORF Names:MGG_02252
OrganismiMagnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri242507 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
Proteomesi
  • UP000009058 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiFungiDB:MGG_02252.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000547822 – 283Tetrahydroxynaphthalene reductaseAdd BLAST282

Interactioni

Subunit structurei

Homotetramer.2 Publications

Structurei

Secondary structure

1283
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 14Combined sources3
Helixi22 – 25Combined sources4
Beta strandi31 – 34Combined sources4
Turni35 – 38Combined sources4
Helixi40 – 51Combined sources12
Beta strandi55 – 62Combined sources8
Helixi64 – 76Combined sources13
Beta strandi81 – 85Combined sources5
Helixi91 – 105Combined sources15
Beta strandi110 – 113Combined sources4
Helixi123 – 125Combined sources3
Helixi128 – 138Combined sources11
Helixi140 – 152Combined sources13
Beta strandi158 – 162Combined sources5
Helixi165 – 167Combined sources3
Helixi176 – 196Combined sources21
Helixi197 – 199Combined sources3
Beta strandi202 – 208Combined sources7
Beta strandi211 – 213Combined sources3
Helixi214 – 219Combined sources6
Helixi220 – 223Combined sources4
Helixi232 – 242Combined sources11
Helixi252 – 263Combined sources12
Helixi265 – 267Combined sources3
Beta strandi274 – 278Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DOHX-ray2.10A/B1-283[»]
1G0NX-ray2.00A/B1-283[»]
1G0OX-ray1.70A/B/C/D1-283[»]
1YBVX-ray2.80A/B1-283[»]
ProteinModelPortaliQ12634.
SMRiQ12634.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12634.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ12634.
KOiK17739.
OrthoDBiEOG092C3TSH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVTQPRGE SKYDAIPGPL GPQSASLEGK VALVTGAGRG IGREMAMELG
60 70 80 90 100
RRGCKVIVNY ANSTESAEEV VAAIKKNGSD AACVKANVGV VEDIVRMFEE
110 120 130 140 150
AVKIFGKLDI VCSNSGVVSF GHVKDVTPEE FDRVFTINTR GQFFVAREAY
160 170 180 190 200
KHLEIGGRLI LMGSITGQAK AVPKHAVYSG SKGAIETFAR CMAIDMADKK
210 220 230 240 250
ITVNVVAPGG IKTDMYHAVC REYIPNGENL SNEEVDEYAA SAWSPLHRVG
260 270 280
LPIDIARVVC FLASNDGGWV TGKVIGIDGG ACM
Length:283
Mass (Da):30,054
Last modified:January 23, 2007 - v2
Checksum:iE079638A96DF19CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22309 mRNA. Translation: AAA19514.1.
AY846878 Genomic DNA. Translation: AAW55623.1.
CM001231 Genomic DNA. Translation: EHA56411.1.
PIRiS41412.
RefSeqiXP_003709023.1. XM_003708975.1.

Genome annotation databases

EnsemblFungiiMGG_02252T0; MGG_02252T0; MGG_02252.
GeneIDi2681349.
KEGGimgr:MGG_02252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22309 mRNA. Translation: AAA19514.1.
AY846878 Genomic DNA. Translation: AAW55623.1.
CM001231 Genomic DNA. Translation: EHA56411.1.
PIRiS41412.
RefSeqiXP_003709023.1. XM_003708975.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DOHX-ray2.10A/B1-283[»]
1G0NX-ray2.00A/B1-283[»]
1G0OX-ray1.70A/B/C/D1-283[»]
1YBVX-ray2.80A/B1-283[»]
ProteinModelPortaliQ12634.
SMRiQ12634.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiMGG_02252T0; MGG_02252T0; MGG_02252.
GeneIDi2681349.
KEGGimgr:MGG_02252.

Organism-specific databases

EuPathDBiFungiDB:MGG_02252.

Phylogenomic databases

InParanoidiQ12634.
KOiK17739.
OrthoDBiEOG092C3TSH.

Enzyme and pathway databases

UniPathwayiUPA00785.

Miscellaneous databases

EvolutionaryTraceiQ12634.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT4HR_MAGO7
AccessioniPrimary (citable) accession number: Q12634
Secondary accession number(s): A4R3G6, G4MQ18, Q5I7G0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.