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Protein

Glucan 1,3-beta-glucosidase

Gene
N/A
Organism
Pichia angusta (Yeast) (Hansenula polymorpha)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Beta-glucanases participate in the metabolism of beta-glucan, the main structural component of the cell wall. It could also function biosynthetically as a transglycosylase (By similarity).By similarity

Catalytic activityi

Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei222 – 2221Proton donorBy similarity
Active sitei323 – 3231NucleophileBy similarity

GO - Molecular functioni

  1. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEXG5A_PICAN.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan 1,3-beta-glucosidase (EC:3.2.1.58)
Alternative name(s):
Exo-1,3-beta-glucanase
OrganismiPichia angusta (Yeast) (Hansenula polymorpha)
Taxonomic identifieri870730 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPichiaceaeOgataea

Subcellular locationi

  1. Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 435405Glucan 1,3-beta-glucosidasePRO_0000007886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi306 ↔ 432By similarity
Disulfide bondi331 ↔ 357By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

SMRiQ12626. Positions 41-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

PhylomeDBiQ12626.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12626-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFPVLHLPK AMKFSSFSLI ASSLLSLVAA APVTLLKRDS RWDYANDKIY
60 70 80 90 100
GVNIGGWLVL EPFITPSLFE AVSSDVPVDE YHYTEALGKE EAEKRLQEHW
110 120 130 140 150
STWIKEEDFK GMANAGLNFV RIPIGYWAFQ LAEGDPYVQG QQEYLDKALE
160 170 180 190 200
WCAKYGLKAW VDLHGAPGSQ NGFDNSGKRG EIGWQNTTGY VDLTVQVLDQ
210 220 230 240 250
LTSKYGGSNY SDVIIGIELL NEPLGSYLDF DQLVDFYNKG YQLVRNNGNA
260 270 280 290 300
PVIIHDAYLP DHTFDNVLNT EQDPNVWEVI VDHHHYQVFD EGSLSQSIDE
310 320 330 340 350
HVSTACGWGQ SENTEYHYSL CGEWTAALTD CAKWLNGAGR GARYDATFGG
360 370 380 390 400
GNYIGSCDQL YTANYDYFTP EVISNYRRYV EAQMDSFLYG KNAGWVFWCW
410 420 430
KTENTIEWDM QRLLGLGIIP QPLDDRQYPN QCGFS
Length:435
Mass (Da):49,264
Last modified:November 1, 1996 - v1
Checksum:i840C22F271CC87A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46868 Genomic DNA. Translation: CAA86948.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46868 Genomic DNA. Translation: CAA86948.1.

3D structure databases

SMRiQ12626. Positions 41-435.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEXG5A_PICAN.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

PhylomeDBiQ12626.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of 1,3-beta-glucanase-encoding genes from non-conventional yeasts."
    Esteban P.F., Vazquez de Aldana C.R., del Rey F.
    Yeast 15:91-109(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14754 / CBS 1976 / JCM 3620 / NBRC 0799 / NCYC 495 / NRRL Y-1798 / VKM Y-1397.

Entry informationi

Entry nameiEXG_PICAN
AccessioniPrimary (citable) accession number: Q12626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.