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Protein

Acyl-CoA desaturase

Gene

Ole1

Organism
Histoplasma capsulatum var. capsulatum
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Stearyl-CoA desaturase that utilizes O2 and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.UniRule annotation

Catalytic activityi

Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Expected to bind 2 Fe2+ ions per subunit.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

Electron transportUniRule annotation, Fatty acid biosynthesisUniRule annotation, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

Keywords - Ligandi

HemeUniRule annotationSAAS annotation, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-CoA desaturaseUniRule annotation (EC:1.14.19.1UniRule annotation)
Gene namesi
Name:Ole1Imported
OrganismiHistoplasma capsulatum var. capsulatumImported
Taxonomic identifieri278162 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesAjellomycetaceaeHistoplasma

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei55 – 7521HelicalSequence analysisAdd
BLAST
Transmembranei82 – 10322HelicalSequence analysisAdd
BLAST
Transmembranei199 – 21719HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Structurei

3D structure databases

ProteinModelPortaliQ12619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini349 – 42779Cytochrome b5 heme-bindingInterPro annotationAdd
BLAST

Domaini

The histidine box domains are involved in binding the catalytic metal ions.UniRule annotation

Sequence similaritiesi

Belongs to the fatty acid desaturase type 1 family.UniRule annotation
Contains cytochrome b5 heme-binding domain.SAAS annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
IPR005804. Fatty_acid_desaturase_dom.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFiPIRSF000345. OLE1. 1 hit.
PRINTSiPR00363. CYTOCHROMEB5.
PR00075. FACDDSATRASE.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNEAPTAT PAAETAAGGK AVTTDTARRP NPEPKKVHIT DTPITLANWH
60 70 80 90 100
KHVSWLNVTL IIVIPIYGLV QAYWVPLHLK TALWAVVYYF MTGLGITAGY
110 120 130 140 150
HRLWAHCSYS ATLPLKIYLA AVGGGAVEGS IRWWARGHRA HHRYTDTDKD
160 170 180 190 200
PYSAHKGLLY SHIGWMVMKQ NPKRIGRTDI TDLNEDPVVV WQHRNYLKVV
210 220 230 240 250
IFMGIVFPML VSGLGWGDWL GGFIYAGILR IFFVQQATFC VNSLAHWLGD
260 270 280 290 300
QPFDDRNSPR DHIVTALVTL GEGYHNFHHE FPSDYRNAIE WHQYDPTKWS
310 320 330 340 350
IWIWKQLGLA YDLKQFRANE IEKGRVQQLQ KKIDQRRVKL DWGIPLDQLP
360 370 380 390 400
VIEWDDYVDQ AKNGRGLIAI AGVVHDVTDF IKDHPGGKAM INTGIGKDAT
410 420 430 440 450
AMFNGGVYNH SNAAHNLLST MRVGVIRGGC EVEIWKRAQK EKKEVESVRD
460 470
EYGNRIVRAG AQVTKIPEPI TTADAA
Length:476
Mass (Da):53,707
Last modified:November 1, 1996 - v1
Checksum:i27A0B152AF621D2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85962 Genomic DNA. Translation: CAA59938.1.
PIRiS52746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85962 Genomic DNA. Translation: CAA59938.1.
PIRiS52746.

3D structure databases

ProteinModelPortaliQ12619.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
IPR005804. Fatty_acid_desaturase_dom.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFiPIRSF000345. OLE1. 1 hit.
PRINTSiPR00363. CYTOCHROMEB5.
PR00075. FACDDSATRASE.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A temperature-senstive strain of Histoplasma capsulatum has an altered D9-fatty acid desaturase gene."
    Gargano S., Di Lallo G., Kobayashi G.S., Maresca B.
    Lipids 30:899-906(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: G217BImported.

Entry informationi

Entry nameiQ12619_AJECA
AccessioniPrimary (citable) accession number: Q12619
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.