true2004-04-132024-03-27103ACO1_AJECAA temperature-sensitive strain of Histoplasma capsulatum has an altered D9-fatty acid desaturase gene.Gargano S.Di Lallo G.Kobayashi G.S.Maresca B.doi:10.1007/bf025374801995Lipids30899-906NUCLEOTIDE SEQUENCE [GENOMIC DNA]FUNCTIONCATALYTIC ACTIVITYATCC 38904 / DownsDelta9-FADS-likeCytochrome b5-like heme/steroid binding domainAcyl-CoA_deSatase_haem/ster-bdAcyl-CoA_DSCyt_B5-like_heme/steroid-bdCyt_B5-like_heme/steroid_sfCyt_B5_heme-BSFA_desaturase_domFADS-1_CSACYL-COA DESATURASEDESATURASE 1, ISOFORM A-RELATEDCyt-b5FA_desaturaseOLE1FACDDSATRASECyt-b5Cytochrome b5-like heme/steroid binding domainCYTOCHROME_B5_1CYTOCHROME_B5_2FATTY_ACID_DESATUR_1Acyl-CoA desaturase1.14.19.1Delta(9)-desaturaseDelta-9 desaturaseFatty acid desaturaseStearoyl-CoA desaturaseOLE1Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Has high specificity and catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides.Expected to bind 2 Fe(2+) ions per subunit.The histidine box domains are involved in binding the catalytic metal ions.Belongs to the fatty acid desaturase type 1 family.Acyl-CoA desaturase537911476Cytoplasmic54Helical5575Lumenal7680Helical81101Cytoplasmic102110Helical111131Lumenal132199Helical200220Cytoplasmic221Cytochrome b5 heme-binding349427Disordered35Histidine box-1106Histidine box-2138142Histidine box-3275279141246278axial binding residue384axial binding residue410121996-11-011537917595105bde3205b7d6f1b3d440e75530MALNEAPTASPVAETAAGGKDVVTDAARRPNSEPKKVHITDTPITLANWHKHISWLNVTLIIAIPIYGLVQAYWVPLHLKTALWAVVYYFMTGLGITAGYHRLWAHCSYSATLPLKIYLAAVGGGAVEGSIRWWARGHRAHHRYTDTDKDPYSVRKGLLYSHIGWMVMKQNPKRIGRTEITDLNEDPVVVWQHRNYLKVVIFMGIVFPMLVSGLGWGDWFGGFIYAGILRIFFVQQATFCVNSLAHWLGDQPFDDRNSPRDHIVTALVTLGEGYHNFHHEFPSDYRNAIEWHQYDPTKWTIWIWKQLGLAYDLKQFRANEIEKGRVQQLQKKIDQRRAKLDWGIPLEQLPVIEWDDYVDQAKNGRGLIAIAGVVHDVTDFIKDHPGGKAMINSGIGKDATAMFNGGVYNHSNAAHNQLSTMRVGVIRGGCEVEIWKRAQKENKEVESVRDEYGNRIVRAGAQVTKIPEPITTADAAtruetruetruetruetruetruetrue