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Q12618

- ACO1_AJECA

UniProt

Q12618 - ACO1_AJECA

Protein

Acyl-CoA desaturase

Gene

OLE1

Organism
Ajellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates.

    Catalytic activityi

    Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.

    Cofactori

    Iron.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi384 – 3841Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi410 – 4101Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. stearoyl-CoA 9-desaturase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-17616.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-CoA desaturase (EC:1.14.19.1)
    Alternative name(s):
    Delta(9)-desaturase
    Short name:
    Delta-9 desaturase
    Fatty acid desaturase
    Stearoyl-CoA desaturase
    Gene namesi
    Name:OLE1
    OrganismiAjellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum)
    Taxonomic identifieri5037 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeOnygenalesAjellomycetaceaeAjellomyces

    Subcellular locationi

    Membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum Source: InterPro
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 476476Acyl-CoA desaturasePRO_0000185404Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ12618.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei55 – 7521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei81 – 10121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei200 – 22021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini349 – 42779Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi101 – 1066Histidine box-1
    Motifi138 – 1425Histidine box-2
    Motifi275 – 2795Histidine box-3

    Domaini

    The histidine box domains may contain the active site and/or be involved in metal ion binding.By similarity

    Sequence similaritiesi

    Belongs to the fatty acid desaturase family.Curated
    Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.10.120.10. 1 hit.
    InterProiIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
    IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    IPR005804. Fatty_acid_desaturase-1.
    IPR001522. Fatty_acid_desaturase-1_C.
    IPR015876. Fatty_acid_desaturase-1_core.
    [Graphical view]
    PfamiPF00173. Cyt-b5. 1 hit.
    PF00487. FA_desaturase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000345. OLE1. 1 hit.
    PRINTSiPR00075. FACDDSATRASE.
    SUPFAMiSSF55856. SSF55856. 1 hit.
    PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    PS00476. FATTY_ACID_DESATUR_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12618-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALNEAPTAS PVAETAAGGK DVVTDAARRP NSEPKKVHIT DTPITLANWH    50
    KHISWLNVTL IIAIPIYGLV QAYWVPLHLK TALWAVVYYF MTGLGITAGY 100
    HRLWAHCSYS ATLPLKIYLA AVGGGAVEGS IRWWARGHRA HHRYTDTDKD 150
    PYSVRKGLLY SHIGWMVMKQ NPKRIGRTEI TDLNEDPVVV WQHRNYLKVV 200
    IFMGIVFPML VSGLGWGDWF GGFIYAGILR IFFVQQATFC VNSLAHWLGD 250
    QPFDDRNSPR DHIVTALVTL GEGYHNFHHE FPSDYRNAIE WHQYDPTKWT 300
    IWIWKQLGLA YDLKQFRANE IEKGRVQQLQ KKIDQRRAKL DWGIPLEQLP 350
    VIEWDDYVDQ AKNGRGLIAI AGVVHDVTDF IKDHPGGKAM INSGIGKDAT 400
    AMFNGGVYNH SNAAHNQLST MRVGVIRGGC EVEIWKRAQK ENKEVESVRD 450
    EYGNRIVRAG AQVTKIPEPI TTADAA 476
    Length:476
    Mass (Da):53,791
    Last modified:November 1, 1996 - v1
    Checksum:iA91A9CE2A865CADB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85963 Genomic DNA. Translation: CAA59939.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85963 Genomic DNA. Translation: CAA59939.1 .

    3D structure databases

    ProteinModelPortali Q12618.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-17616.

    Family and domain databases

    Gene3Di 3.10.120.10. 1 hit.
    InterProi IPR009160. Acyl-CoA_deSatase_haem/ster-bd.
    IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    IPR005804. Fatty_acid_desaturase-1.
    IPR001522. Fatty_acid_desaturase-1_C.
    IPR015876. Fatty_acid_desaturase-1_core.
    [Graphical view ]
    Pfami PF00173. Cyt-b5. 1 hit.
    PF00487. FA_desaturase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000345. OLE1. 1 hit.
    PRINTSi PR00075. FACDDSATRASE.
    SUPFAMi SSF55856. SSF55856. 1 hit.
    PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    PS00476. FATTY_ACID_DESATUR_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A temperature-senstive strain of Histoplasma capsulatum has an altered D9-fatty acid desaturase gene."
      Gargano S., Di Lallo G., Kobayashi G.S., Maresca B.
      Lipids 30:899-906(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 38904 / Downs.

    Entry informationi

    Entry nameiACO1_AJECA
    AccessioniPrimary (citable) accession number: Q12618
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3