Reviewed, UniProtKB/Swiss-Prot Q12618 (ACO1_AJECA)

Names and origin

Protein names

Recommended name:
    Acyl-CoA desaturase
    EC=1.14.19.1
Alternative name(s):
    Stearoyl-CoA desaturase
    Fatty acid desaturase
    Delta(9)-desaturase

Gene names

Name:

OLE1

Organism

Ajellomyces capsulata (Darling's disease fungus) (Histoplasma capsulatum)

Taxonomic identifier

5037 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates.
Catalytic activity	Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.
Cofactor	Iron By similarity.
Subcellular location	Membrane; Multi-pass membrane protein By similarity.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding By similarity.
Sequence similarities	Belongs to the fatty acid desaturase family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Fatty acid biosynthesis Lipid synthesis Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro stearoyl-CoA 9-desaturase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Acyl-CoA desaturase		PRO_0000185404
Regions
	Transmembrane	55 – 75	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	200 – 220	21	Potential
	Domain	349 – 427	79	Cytochrome b5 heme-binding
	Motif	101 – 106	6	Histidine box-1
	Motif	138 – 142	5	Histidine box-2
	Motif	275 – 279	5	Histidine box-3
Sites
	Metal binding	384	1	Iron (heme axial ligand) By similarity
	Metal binding	410	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12618-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A91A9CE2A865CADB	FASTA	476	53,791
	10 20 30 40 50 60 MALNEAPTAS PVAETAAGGK DVVTDAARRP NSEPKKVHIT DTPITLANWH KHISWLNVTL 70 80 90 100 110 120 IIAIPIYGLV QAYWVPLHLK TALWAVVYYF MTGLGITAGY HRLWAHCSYS ATLPLKIYLA 130 140 150 160 170 180 AVGGGAVEGS IRWWARGHRA HHRYTDTDKD PYSVRKGLLY SHIGWMVMKQ NPKRIGRTEI 190 200 210 220 230 240 TDLNEDPVVV WQHRNYLKVV IFMGIVFPML VSGLGWGDWF GGFIYAGILR IFFVQQATFC 250 260 270 280 290 300 VNSLAHWLGD QPFDDRNSPR DHIVTALVTL GEGYHNFHHE FPSDYRNAIE WHQYDPTKWT 310 320 330 340 350 360 IWIWKQLGLA YDLKQFRANE IEKGRVQQLQ KKIDQRRAKL DWGIPLEQLP VIEWDDYVDQ 370 380 390 400 410 420 AKNGRGLIAI AGVVHDVTDF IKDHPGGKAM INSGIGKDAT AMFNGGVYNH SNAAHNQLST 430 440 450 460 470 MRVGVIRGGC EVEIWKRAQK ENKEVESVRD EYGNRIVRAG AQVTKIPEPI TTADAA

References

[1]	"A temperature-senstive strain of Histoplasma capsulatum has an altered D9-fatty acid desaturase gene." Gargano S., Di Lallo G., Kobayashi G.S., Maresca B. Lipids 30:899-906(1995) [PubMed: 8538376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38904 / Downs.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X85963 Genomic DNA. Translation: CAA59939.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.19.1. 266116.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. IPR005804. Fatty_acid_desaturase-1. IPR001522. Fatty_acid_desaturase-1_C. IPR015876. Fatty_acid_desaturase-1_core. IPR009160. OLE1_hae_ster_bd. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. PF00487. FA_desaturase. 1 hit. [Graphical view]
PIRSF	PIRSF000345. OLE1. 1 hit.
PRINTS	PR00075. FACDDSATRASE.
ProDom	PD000612. Cyt_B5. 1 hit. PD002221. Desaturase. 1 hit. PD001081. FA_desat_sub. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. PS00476. FATTY_ACID_DESATUR_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACO1_AJECA

Accession

Primary (citable) accession number: Q12618

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)