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Q12618 (ACO1_AJECA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acyl-CoA desaturase
EC=1.14.19.1
Alternative name(s):
Delta(9)-desaturase
Short name=Delta-9 desaturase
Fatty acid desaturase
Stearoyl-CoA desaturase
Gene names
Name:OLE1
OrganismAjellomyces capsulatus (Darling's disease fungus) (Histoplasma capsulatum)
Taxonomic identifier5037 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Utilizes O2 and electrons from the reduced cytochrome b5 domain to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates.

Catalytic activity

Stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O.

Cofactor

Iron By similarity.

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding By similarity.

Sequence similarities

Belongs to the fatty acid desaturase family.

Contains 1 cytochrome b5 heme-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Acyl-CoA desaturase
PRO_0000185404

Regions

Transmembrane55 – 7521Helical; Potential
Transmembrane81 – 10121Helical; Potential
Transmembrane200 – 22021Helical; Potential
Domain349 – 42779Cytochrome b5 heme-binding
Motif101 – 1066Histidine box-1
Motif138 – 1425Histidine box-2
Motif275 – 2795Histidine box-3

Sites

Metal binding3841Iron (heme axial ligand) By similarity
Metal binding4101Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12618 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A91A9CE2A865CADB

FASTA47653,791
        10         20         30         40         50         60 
MALNEAPTAS PVAETAAGGK DVVTDAARRP NSEPKKVHIT DTPITLANWH KHISWLNVTL 

        70         80         90        100        110        120 
IIAIPIYGLV QAYWVPLHLK TALWAVVYYF MTGLGITAGY HRLWAHCSYS ATLPLKIYLA 

       130        140        150        160        170        180 
AVGGGAVEGS IRWWARGHRA HHRYTDTDKD PYSVRKGLLY SHIGWMVMKQ NPKRIGRTEI 

       190        200        210        220        230        240 
TDLNEDPVVV WQHRNYLKVV IFMGIVFPML VSGLGWGDWF GGFIYAGILR IFFVQQATFC 

       250        260        270        280        290        300 
VNSLAHWLGD QPFDDRNSPR DHIVTALVTL GEGYHNFHHE FPSDYRNAIE WHQYDPTKWT 

       310        320        330        340        350        360 
IWIWKQLGLA YDLKQFRANE IEKGRVQQLQ KKIDQRRAKL DWGIPLEQLP VIEWDDYVDQ 

       370        380        390        400        410        420 
AKNGRGLIAI AGVVHDVTDF IKDHPGGKAM INSGIGKDAT AMFNGGVYNH SNAAHNQLST 

       430        440        450        460        470 
MRVGVIRGGC EVEIWKRAQK ENKEVESVRD EYGNRIVRAG AQVTKIPEPI TTADAA

« Hide

References

[1]"A temperature-senstive strain of Histoplasma capsulatum has an altered D9-fatty acid desaturase gene."
Gargano S., Di Lallo G., Kobayashi G.S., Maresca B.
Lipids 30:899-906(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 38904 / Downs.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X85963 Genomic DNA. Translation: CAA59939.1.

3D structure databases

ProteinModelPortalQ12618.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17616.

Family and domain databases

Gene3D3.10.120.10. 1 hit.
InterProIPR009160. Acyl-CoA_deSatase_haem/ster-bd.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR005804. Fatty_acid_desaturase-1.
IPR001522. Fatty_acid_desaturase-1_C.
IPR015876. Fatty_acid_desaturase-1_core.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFPIRSF000345. OLE1. 1 hit.
PRINTSPR00075. FACDDSATRASE.
SUPFAMSSF55856. Cyt_B5. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS00476. FATTY_ACID_DESATUR_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACO1_AJECA
AccessionPrimary (citable) accession number: Q12618
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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