Q12612 (TRI4_FUSSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trichodiene oxygenase
EC=1.14.-.-

Alternative name(s):
Cytochrome P450 58

Gene names

Name:	TRI4
Synonyms:	CYP58, TOX4

Organism

Fusarium sporotrichioides

Taxonomic identifier

5514 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › mitosporic Nectriaceae › Fusarium

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxygenation of trichodiene to yield unknown reaction product.
Cofactor	Heme group By similarity.
Pathway	Sesquiterpene biosynthesis; trichothecene biosynthesis.
Miscellaneous	Trichothecenes are sesquiterpenoid toxins that act by inhibiting protein biosynthesis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Trichodiene oxygenase		PRO_0000052045
Sites
	Metal binding	455	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12612 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2B1EC16B12573C83	FASTA	520	59,074
	10 20 30 40 50 60 MVDQDWIKAL VNIPISHAVG VVAASTVIYF LSSCFYNLYL HPLRKIPGPK LAAIGPYLEF 70 80 90 100 110 120 YHEVIRDGQY LWEIAKMHDK YGPIVRVNDK EVHIRDPSYY STIYTAGARK TNKDPATVGA 130 140 150 160 170 180 FDVPTATAAT VDHDHHRARR GYLNPYFSKR SITNLEPFIH ERVTKLLSRF QEHLDNDQVL 190 200 210 220 230 240 SLDGAFCALT ADVITSRFYG KHYNYLDLPD FHFVVRDGFL GLTKVYHLAR FIPVLVTVLK 250 260 270 280 290 300 RLPYSCLRLI APSVSDLLQM RNEIHERGGD EFLSSKTSEA KSSILFGALA DTHIPPVERT 310 320 330 340 350 360 VERMLDEGTV ILFAGTETTS RTLAITFFYL LTHPECLRKL REELNSLPKV EGDRFPLATL 370 380 390 400 410 420 ENLPYLNGVV HEGFRLACGP ISRSGRVATQ ENLKYKEHVI PAGTPVSQST YFMHTDPKIF 430 440 450 460 470 480 PEPEKFKPER WIEAAEKKIP LKKYITNFSQ GSRQCIGYTM AFAEMYLAMS RIARAYDVEL 490 500 510 520 YDTTKADIDM THARIVAYPK AIPGKTEHVG EIRVKVLKAL

References

[1]	"The Tri4 gene of Fusarium sporotrichioides encodes a cytochrome P450 monooxygenase involved in trichothecene biosynthesis." Hohn T.M., Desjardins A.E., McCormick S.P. Mol. Gen. Genet. 248:95-102(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL 3299.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22462 Genomic DNA. Translation: AAB72032.1.
PIR	S57337.
3D structure databases
ProteinModelPortal	Q12612.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00267.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRI4_FUSSP

Accession

Primary (citable) accession number: Q12612

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program