Q12594 (DMAW_CLAFS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan dimethylallyltransferase
EC=2.5.1.34

Alternative name(s):
4-dimethylallyltryptophan synthase
All-trans-hexaprenyl-diphosphate synthase
L-tryptophan dimethylallyl transferase
Short name=DMATS

Gene names

Name:

dmaW

Organism

Claviceps fusiformis (Ergot fungus)

Taxonomic identifier

40602 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Clavicipitaceae › Claviceps

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the first step of ergot alkaloid biosynthesis. Ergot alkaloids, which are produced by endophyte fungi, can enhance plant host fitness, but also cause livestock toxicosis to host plants.
Catalytic activity	Dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan. Ref.1 Ref.2
Pathway	Alkaloid biosynthesis; ergot alkaloid biosynthesis.
Subunit structure	Homodimer. Ref.2
Sequence similarities	Belongs to the tryptophan dimethylallyltransferase family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tryptophan dimethylallyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Tryptophan dimethylallyltransferase		PRO_0000181363

Sequences

	Sequence		Length	Mass (Da)
	Q12594 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A913F8E60EBBEB63	FASTA	455	51,858
	10 20 30 40 50 60 MMTKAPATAV YDTLSLLFDF PNQEQRLWWH SIAPMFAAML DTAGHNVHDQ YRHLGIFKKH 70 80 90 100 110 120 IIPFLGVYPA QGKHTWPSVL TRYGIPFELS LNCLDSVVRY TFEPTTEHTG TGDDSYNAFA 130 140 150 160 170 180 ILECIQKLVR IQPGIDMEWF SYFRNELVLN ATESARLGRN DSVNQQPIRT QNKLALDLKG 190 200 210 220 230 240 DRFALKVYLY PHLKSIATGV SSHDLIFNSV RKLSQKHTSI QPSFNVLCDY VASRNDPDSN 250 260 270 280 290 300 AAEAEAGVPA SALRARLLSC DLVDPSKSRI KIYLLEQTVS LTAMEDLWTL GGRRTDSSTL 310 320 330 340 350 360 NGLDMMRELW HLLQIPSGFM KYPESDLKLG EVPDEQLPSM VHYALHPDQP MPEPQVYFTV 370 380 390 400 410 420 FGMSDAGITN ALATFFSRHG WYEMAKKYRV FLEGSFPNHD FESLNYLHTY VSFSYRKNKP 430 440 450 YLSVYLHSFE TGQWPAFSDD PTAFNAFKRC DLSLT

References

[1]	"The Claviceps purpurea gene encoding dimethylallyltryptophan synthase, the committed step for ergot alkaloid biosynthesis." Tsai H.-F., Wang H., Gebler J.C., Poulter C.D., Schardl C.L. Biochem. Biophys. Res. Commun. 216:119-125(1995) [PubMed: 7488077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 340-349; 351-360 AND 384-407, ENZYME ACTIVITY.
[2]	"Purification and characterization of dimethylallyl tryptophan synthase from Claviceps purpurea." Gebler J.C., Poulter C.D. Arch. Biochem. Biophys. 296:308-313(1992) [PubMed: 1605639] [Abstract] Cited for: ENZYME ACTIVITY, HOMODIMERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L39640 Genomic DNA. Translation: AAC18893.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017795. Aromatic_prenylTrfase_DMATS. IPR012148. Trp_dimethylallyltransferase. IPR017796. Trp_dimethylallylTrfase_sub. [Graphical view]
Pfam	PF11991. Trp_DMAT. 1 hit. [Graphical view]
PIRSF	PIRSF000509. Trp_DMAT. 1 hit.
TIGRFAMs	TIGR03429. Arom_pren_DMATS. 1 hit. TIGR03430. Trp_dimet_allyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DMAW_CLAFS

Accession

Primary (citable) accession number: Q12594

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 1, 1996
Last modified:	October 19, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program