Reviewed, UniProtKB/Swiss-Prot Q12577 (HMDH_GIBFU)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase
      Short name=HMG-CoA reductase
    EC=1.1.1.34

Gene names

Name:	HMGR
Synonyms:	HMGA

Organism

Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium moniliforme)

Taxonomic identifier

5127 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › Gibberella › Gibberella fujikuroi complex

Protein attributes

Sequence length	976 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of the sterol biosynthesis.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 976	976	3-hydroxy-3-methylglutaryl-coenzyme A reductase		PRO_0000114452
Regions
	Transmembrane	37 – 57	21	Potential
	Transmembrane	65 – 85	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	194 – 214	21	Potential
	Transmembrane	274 – 294	21	Potential
	Transmembrane	402 – 422	21	Potential
	Region	423 – 517	95	Linker
	Region	518 – 976	459	Catalytic
Sites
	Active site	618	1	Charge relay system By similarity
	Active site	752	1	Charge relay system By similarity
	Active site	828	1	Charge relay system By similarity
	Active site	924	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	451	1	N-linked (GlcNAc...) Potential
	Glycosylation	498	1	N-linked (GlcNAc...) Potential
	Glycosylation	682	1	N-linked (GlcNAc...) Potential
	Glycosylation	928	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	686	1	R → S in CAA41261. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q12577-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2B8F1C9F3CB5A714	FASTA	976	105,395
	10 20 30 40 50 60 MDHEGCQGQH PQQCCQWVSN AWSEFLDLLK NAETLDIVIM LLGYIAMHLT FVSLFLSMRK 70 80 90 100 110 120 MGSKFWLGIC TLFSSVFAFL FGLVVTTKLG VPISVILLSE GLPFLVVTIG FEKNIVLTRA 130 140 150 160 170 180 VMSHAIEHRR IQAQNSKSGK RSPDGSTQNM IQYAVQAAIK EKGFEIIRDY AIEIVILVIG 190 200 210 220 230 240 AASGVQGGLQ QFCFLAAWTL FFDFILLFTF YTAILSIKLR STVSSVMSIC VWPLRMMASR 250 260 270 280 290 300 RVAENVAKGD DELNRVRGDA PLFGRKSSSI PKFKVLMILG FIFVNIVNIC SIPFRNPSSM 310 320 330 340 350 360 STIRTWASSL GGVIAPLSVD PFKVASNGLD AILPTAKSNN RPTLVTVLTP IKYELEYPSI 370 380 390 400 410 420 HYALGSAASN PAYNDAFHHH FQGYGVGGRM VGGILKSLED PVLSKWIVIA LALSVALNGY 430 440 450 460 470 480 LFNVARWGIK DPNVPEHNID RNELARAREF NDTGSATLPL GEYVPPTPMR TQPSTPAITD 490 500 510 520 530 540 DEAEGLHMTK ARPANLPNRS NEELEKLLSE NALREMTDEE VISLSMRGKI PGYALEKTLG 550 560 570 580 590 600 DFTRAVKIRR SIIARNKAAA DITHSLDRSK LPYENYNWER FFGACCENVI GYMPLPVGVA 610 620 630 640 650 660 GPLVIDGQSY FIPMATTEGV LVASASRGCK AINSGGGAIT VLTADGMTRG PCVAFETLER 670 680 690 700 710 720 AGAAKLWLDS EAGQDMMKKA FNSTSRFARL QSMKTALAGT NLYIRFKTTT GDAMGMNMIS 730 740 750 760 770 780 KGVEHALSVM ANDGGFDDMQ IISVSGNYCT DKKAAALNWI DGRGKGVVAE AIIPGEVVRS 790 800 810 820 830 840 VLKSDVDSLV ELNVAKNLIG SAMAGSVGGF NAHAANIVAA IFLATGQDPA QVVESANCIT 850 860 870 880 890 900 IMKNLNGALQ ISVSMPSLEV GTLGGGTILE PQGAMLDILG VRGSHPTNPG DNARRLARII 910 920 930 940 950 960 GAAVLAGELS LCSALAAGHL VRAHMQHNRS AAPSRSTTPG SSHDARLTGH DQCPRALSVN 970 NVDERRRYSE VKAIDE

References

[1]	"3-hydroxy-3-methylglutaryl-CoA reductase gene of Gibberella fujikuroi: isolation and characterization." Woitek S., Unkles S.E., Kinghorn J.R., Tudzynski B. Curr. Genet. 31:38-47(1997) [PubMed: 9000379] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: m567.
[2]	"Cloning a segment of the gene encoding 3-hydroxy-3-methyglutaryl coenzyme A reductase in Phycomyces blakesleeanus and Gibberella fujikuroi." Corrochano L.M., Avalos J. Exp. Mycol. 16:167-171(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 613-718. Strain: IMI 58289.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94307 Genomic DNA. Translation: CAA63970.1. X58370 Genomic DNA. Translation: CAA41261.1.
PIR	S17343.
3D structure databases
HSSP	HSSP built from PDB template 1DQA based on UniProtKB P04035.
ModBase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR000731. SSD_5TM. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. PS50156. SSD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH_GIBFU

Accession

Primary (citable) accession number: Q12577
Secondary accession number(s): Q12615

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)