Q12570 (LAC1_BOTFU) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 70.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Laccase-1 EC=1.10.3.2 Alternative name(s): Benzenediol:oxygen oxidoreductase 1 Diphenol oxidase 1 Urishiol oxidase 1 | ||||
| Gene names |
| ||||
| Organism | Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea) | ||||
| Taxonomic identifier | 40559 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Botryotinia![]() |
Protein attributes
| Sequence length | 561 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Lignin degradation and detoxification of lignin-derived products By similarity. |
| Catalytic activity | 4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O. |
| Cofactor | Binds 4 copper ions per monomer By similarity. |
| Subcellular location | Secreted Potential. |
| Induction | Not induced by resveratrol or tannins. Ref.1 |
| Sequence similarities | Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains. |
| Sequence caution | The sequence AAB41823.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence does probably not originate from mRNA or it was not properly spliced. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lignin degradation |
| Cellular component | Secreted |
| Domain | Repeat Signal |
| Ligand | Copper Metal-binding |
| Molecular function | Oxidoreductase |
| PTM | Glycoprotein |
| Gene Ontology (GO) | |
| Biological_process | lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | copper ion binding Inferred from electronic annotation. Source: InterPro hydroquinone:oxygen oxidoreductase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 20 | 20 | Potential | ||||||
| Chain | 21 – 561 | 541 | Laccase-1 | PRO_0000085584 | |||||
Regions | |||||||||
| Domain | 68 – 185 | 118 | Plastocyanin-like 1 | ||||||
| Domain | 191 – 337 | 147 | Plastocyanin-like 2 | ||||||
| Domain | 396 – 525 | 130 | Plastocyanin-like 3 | ||||||
Sites | |||||||||
| Metal binding | 119 | 1 | Copper 1; type 2 By similarity | ||||||
| Metal binding | 121 | 1 | Copper 2; type 3 By similarity | ||||||
| Metal binding | 163 | 1 | Copper 2; type 3 By similarity | ||||||
| Metal binding | 165 | 1 | Copper 3; type 3 By similarity | ||||||
| Metal binding | 445 | 1 | Copper 4; type 1 By similarity | ||||||
| Metal binding | 448 | 1 | Copper 1; type 2 By similarity | ||||||
| Metal binding | 450 | 1 | Copper 3; type 3 By similarity | ||||||
| Metal binding | 504 | 1 | Copper 3; type 3 By similarity | ||||||
| Metal binding | 505 | 1 | Copper 4; type 1 By similarity | ||||||
| Metal binding | 506 | 1 | Copper 2; type 3 By similarity | ||||||
| Metal binding | 510 | 1 | Copper 4; type 1 By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 71 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 87 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 114 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 226 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 284 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 327 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 391 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 398 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 117 – 118 | 2 | SI → TM in AAB41823. Ref.2 | ||||||
| Sequence conflict | 372 | 1 | V → I in AAB41823. Ref.2 | ||||||
| Sequence conflict | 429 | 1 | N → K in AAB41823. Ref.2 | ||||||
| Sequence conflict | 481 | 1 | M → L in AAB41823. Ref.2 | ||||||
| Sequence conflict | 484 | 1 | P → S in AAB41823. Ref.2 | ||||||
| Sequence conflict | 511 | 1 | A → V in AAB41823. Ref.2 | ||||||
| Sequence conflict | 547 | 1 | A → G in AAB41823. Ref.2 | ||||||
Sequences
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References
| [1] | "Resveratrol acts as a natural profungicide and induces self-intoxication by a specific laccase." Schouten A., Wagemakers L., Stefanato F.L., van der Kaaij R.M., van Kan J.A.L. Mol. Microbiol. 43:883-894(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: SAS56. |
| [2] | "A laccase cDNA from Botrytis cinerea." Cantone F.A., Staples R.C. Phytopathology 83:1383-1383(1993) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-561. Strain: SAS56. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF243854 Genomic DNA. Translation: AAK77952.1. U20192 mRNA. Translation: AAB41823.1. Sequence problems. |
3D structure databases | |
| ProteinModelPortal | Q12570. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| Gene3D | 2.60.40.420. 3 hits. |
| InterPro | IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. [Graphical view] |
| Pfam | PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view] |
| SUPFAM | SSF49503. Cupredoxin. 3 hits. |
| PROSITE | PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LAC1_BOTFU | ||||||||
| Accession | Primary (citable) accession number: Q12570 Secondary accession number(s): Q96WN0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with
