Q12564 (CHSA_AMPQU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase A
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase A
Class-I chitin synthase A

Gene names

Name:

CHSA

Organism

Ampelomyces quisqualis

Taxonomic identifier

50730 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Pleosporomycetidae › Pleosporales › Pleosporineae › Leptosphaeriaceae › mitosporic Leptosphaeriaceae › Ampelomyces

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class I subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 910	910	Chitin synthase A		PRO_0000193679
Regions
	Transmembrane	366 – 386	21	Helical; Potential
	Transmembrane	448 – 468	21	Helical; Potential
	Transmembrane	583 – 603	21	Helical; Potential
	Transmembrane	620 – 640	21	Helical; Potential
	Transmembrane	655 – 675	21	Helical; Potential
	Transmembrane	701 – 721	21	Helical; Potential
	Transmembrane	730 – 750	21	Helical; Potential
	Transmembrane	828 – 848	21	Helical; Potential
	Transmembrane	876 – 896	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12564 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F8F1DB135F2138F9	FASTA	910	103,013
	10 20 30 40 50 60 MARRQGPSRS PWAPTQRRRL ATDCPCSRVY VLAHTAAAVA NSEQYSVENI PYAGGNYHDD 70 80 90 100 110 120 YDPRPGGRRH DSDYSLDPNA HHDAYYSQPY DPAPHDASPG GAYGTQPDHY WQDDDVGRPM 130 140 150 160 170 180 IQGANAYGPD PHDPDRDPHP DDPFHDEPAP TPTPAPIKRW KTVKEVQLFK GNLVLDCPIP 190 200 210 220 230 240 PRLLNQVPHA QPPERDEFTH MRYSAATCDP ADFDAERFTL RQKLFAKPRH TELFIVITMY 250 260 270 280 290 300 NEEDELFART MTGVIKNIEY MNSRTNSKTW GKDAWKKIVV CVVSDGRAKI NPRTRAVLAA 310 320 330 340 350 360 LGVYQDGIAK QQVNGKDVTA HIYEYTTQMT LDIKKGVVGV KKGNTPVQML FCLKEKNQKK 370 380 390 400 410 420 INSHRWFFQA FGQVLDPNIC VLIDAGTKPG KDSVYQLWKA FDLEPMCAGA CGEIKAMLVH 430 440 450 460 470 480 GKKLLNPLVA TQNFEYKMSN ILDKPLESAF GFITVLPGAF SAYRYVALQN DKAGQGPLEK 490 500 510 520 530 540 YFAGEKMHGA NAGIFTANMY LAEDRILCFE LVSKRNCHWI LQYVKSATGE TDVPTTMAEF 550 560 570 580 590 600 ISQRRRWLNG SFFAAVYALA HSFDIFRSDH SFLRKTMFLV EFVYQTISML FAWFALGNFF 610 620 630 640 650 660 LVFRILTASL QNELGTAGKV LFIVFEWLYI AVLITCFILS LGNRPQGSNK WYMSMVYFWG 670 680 690 700 710 720 VIMAYLMFAS IFITVRSIQS QIRNNGGFNA SDLLKDKIFA TLIISLLSTY VMWLVVSIIF 730 740 750 760 770 780 LDPWHMFTSF IQYLLMTPTY INILNVYAFC NTHDITWGTK GDDKPEKLPS VTTKADGKAD 790 800 810 820 830 840 IHAPTDDADL NTQYETELHV FSTKWKEEKK VPSPGEKQED YYRGFRSGVV LFWMFCNLAL 850 860 870 880 890 900 TALVLQAGGL ELTVSDPDEA QEKQNQASTI YLAVVLYSVA GLAAFRFIGA MWFLVVRMVS 910 ATCRFTSPIY

References

[1]	"The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis." Weiss N., Sztejnberg A., Yarden O. Gene 168:99-102(1996) [PubMed: 8626074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AQ10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86802 Genomic DNA. Translation: CAA60497.1.
PIR	JC4609.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHSA_AMPQU

Accession

Primary (citable) accession number: Q12564

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program