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Protein

Nitrate reductase

Gene

niaD

Organism
Aspergillus oryzae (Yellow koji mold)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADSAAS annotation
  • Mo-molybdopterinSAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseSAAS annotationImported

Keywords - Biological processi

Nitrate assimilationUniRule annotation

Keywords - Ligandi

FADSAAS annotation, Flavoprotein, HemeSAAS annotation, Iron, Metal-binding, MolybdenumSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductaseUniRule annotation
Gene namesi
Name:niaDImported
OrganismiAspergillus oryzae (Yellow koji mold)Imported
Taxonomic identifieri5062 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Interactioni

Subunit structurei

Homodimer.SAAS annotation

Protein-protein interaction databases

STRINGi5062.CADAORAP00006062.

Structurei

3D structure databases

ProteinModelPortaliQ12561.
SMRiQ12561. Positions 40-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini514 – 58976Cytochrome b5 heme-bindingInterPro annotationAdd
BLAST
Domaini615 – 727113FAD-binding FR-typeInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.UniRule annotation
Contains FAD-binding FR-type domain.SAAS annotation
Contains cytochrome b5 heme-binding domain.SAAS annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATITEVRTD ALVPTDLVLK TGQIKIESEE ISTRDLSDIP LPPPSKRPTE
60 70 80 90 100
VLSVDKGTPD SHVPRDPRLI RLTGVHPFNV EPPLTDLYKE GFLTSPELFY
110 120 130 140 150
VRNHGPVPHI KDEDIPHWEI SIEGLVEKPL VLNFRQVLQQ YDQITAPITL
160 170 180 190 200
VCAGNRRKEQ NNVRKTKGFS WGSAGLSTAL FTGPLLADIL RSAKPLRKAK
210 220 230 240 250
YVCMEGADKL PNGHYGTSIK LNWALDPNRG IMLAHKMNGE SLRPDHGRPL
260 270 280 290 300
RAVVPGQIGG RSVKWLKRLI LTDAPSDNWY HINDNRVLPT MVSPEMASNN
310 320 330 340 350
RNWWHDERYA IYDLNTNSAV AYPQNNEVLN LLVAGPSYTV RGYAYAGGGR
360 370 380 390 400
RVTRVEISLD KGKSWRLAEI EYAEDKYRDF EGELFGGKVD MYWRETCFCW
410 420 430 440 450
CFWSLSITIP ELENSDAILV RAMDEALGVQ PRDMYWSVLG MMNNPWFRVT
460 470 480 490 500
ITKENGNLRF EHPTHPSMPT GWMERVKKAG GDLTNGNWGE RHEGEEPTEP
510 520 530 540 550
EPVQDINMKK DGLSRVIGFE EFKENSCDEK PWFIVNGEVY DGQAFLEGHP
560 570 580 590 600
GGAQSIISSA GLDVSEEFLA IHSETAKAMM PEYHIGTMDP EGLKALKDDA
610 620 630 640 650
SSSTDEIRPV FLQSRSWTKA TLKERKDISW DTRIFSFKLE HEDQTLGLPV
660 670 680 690 700
GQHLMIKVLD TSSNNEAIIR SYTPISETSQ KGTVDLLVKV YFATATSAGG
710 720 730 740 750
KMTMALDRLP LGSVVECKGP TGRFEYLGNG RVVISGKERH VRSFKMICGG
760 770 780 790 800
TGITPIFQVL RAVVQDRQDP TSCTVLNGNR QEEDILCRAE LDGFMATDSR
810 820 830 840 850
RCNIIHTLSK APDSWTGRRG RISEELLKEY AAPEDESMVL ICGPPAMEES
860
ARRILLAEGW KESDLHFF
Length:868
Mass (Da):97,531
Last modified:November 1, 1996 - v1
Checksum:i3C881039B7614C77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49701 Genomic DNA. Translation: BAA08551.1.
PIRiJC4283.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49701 Genomic DNA. Translation: BAA08551.1.
PIRiJC4283.

3D structure databases

ProteinModelPortaliQ12561.
SMRiQ12561. Positions 40-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5062.CADAORAP00006062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
COG0543. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
PR00371. FPNCR.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQ12561_ASPOZ
AccessioniPrimary (citable) accession number: Q12561
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.