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Protein

Enolase

Gene

enoA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gpdA), Glyceraldehyde-3-phosphate dehydrogenase (AO090011000414)
  2. Phosphoglycerate kinase (pgkA)
  3. no protein annotated in this organism
  4. Enolase (enoA)
  5. Pyruvate kinase (AO090005001556)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei159SubstrateBy similarity1
Binding sitei168SubstrateBy similarity1
Active sitei211Proton donorBy similarity1
Metal bindingi246MagnesiumBy similarity1
Metal bindingi297MagnesiumBy similarity1
Binding sitei297SubstrateBy similarity1
Metal bindingi322MagnesiumBy similarity1
Binding sitei322SubstrateBy similarity1
Active sitei347Proton acceptorBy similarity1
Binding sitei398SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:enoA
ORF Names:AO090003000055
OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Taxonomic identifieri510516 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000006564 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340421 – 438EnolaseAdd BLAST438

Proteomic databases

PRIDEiQ12560.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ12560.
SMRiQ12560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni374 – 377Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
KOiK01689.
OMAiSKWGGKG.
OrthoDBiEOG092C2W5X.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPITKIHARS VYDSRGNPTV EVDVVTETGL HRAIVPSGAS TGQHEAHELR
60 70 80 90 100
DGDKTHWGGK GVLKAVENVN KTIAPAVIEE NLDVKDQSKV DEFLKKLDGS
110 120 130 140 150
ANKSNLGANA ILGVSLAIAK AGAAEKGVPL YAHISDLAGT KKPYVLPVPF
160 170 180 190 200
QNVLNGGSHA GGRLAFQEFM IVPSAAPSFS EALRQGAEVY QKLKTLAKKK
210 220 230 240 250
YGQSAGNVGD EGGVAPDIQT AEEALDLITE AIEQAGYTGK MKIAMDVASS
260 270 280 290 300
EFYKADVKKY DLDFKNPDSD SSKWLTYEQL ADLYKTLASK YPIVSIEDPF
310 320 330 340 350
AEDDWEAWSY FYKTSDFQIV GDDLTVTNPL RIKKAIETKA CNALLLKVNQ
360 370 380 390 400
IGTLTESIQA AKDSYADNWG VMVSHRSGET EDVTIADIAV GLRSGQIKTG
410 420 430
APARSERLAK LNQILRIEEE LGNNAIYAGE KFRTSVNL
Length:438
Mass (Da):47,406
Last modified:November 1, 1996 - v1
Checksum:i1333325EEAEABBE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63941 Genomic DNA. Translation: BAA09973.1.
D64113 mRNA. Translation: BAA23760.1.
AP007155 Genomic DNA. Translation: BAE57291.1.
PIRiJC4542.
RefSeqiXP_001819293.1. XM_001819241.2.

Genome annotation databases

EnsemblFungiiBAE57291; BAE57291; AO090003000055.
GeneIDi5991276.
KEGGiaor:AOR_1_96154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63941 Genomic DNA. Translation: BAA09973.1.
D64113 mRNA. Translation: BAA23760.1.
AP007155 Genomic DNA. Translation: BAE57291.1.
PIRiJC4542.
RefSeqiXP_001819293.1. XM_001819241.2.

3D structure databases

ProteinModelPortaliQ12560.
SMRiQ12560.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ12560.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAE57291; BAE57291; AO090003000055.
GeneIDi5991276.
KEGGiaor:AOR_1_96154.

Phylogenomic databases

HOGENOMiHOG000072174.
KOiK01689.
OMAiSKWGGKG.
OrthoDBiEOG092C2W5X.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_ASPOR
AccessioniPrimary (citable) accession number: Q12560
Secondary accession number(s): Q2UMC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 12, 2017
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.