Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q12558

- AGLU_ASPOR

UniProt

Q12558 - AGLU_ASPOR

Protein

Alpha-glucosidase

Gene

agdA

Organism
Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes malto-oligosaccharides, but has a low activity toward soluble starch.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei492 – 4921NucleophilePROSITE-ProRule annotation
    Active sitei495 – 4951By similarity
    Active sitei660 – 6601Proton donorBy similarity

    GO - Molecular functioni

    1. alpha-1,4-glucosidase activity Source: ASPGD
    2. carbohydrate binding Source: InterPro
    3. glucosyltransferase activity Source: ASPGD
    4. maltose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular polysaccharide metabolic process Source: ASPGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH31. Glycoside Hydrolase Family 31.
    mycoCLAPiAGL31A_ASPOR.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-glucosidase (EC:3.2.1.20)
    Short name:
    AGL
    Alternative name(s):
    Maltase
    Gene namesi
    Name:agdA
    ORF Names:AO090003001209
    OrganismiAspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
    Taxonomic identifieri510516 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000006564: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525By similarityAdd
    BLAST
    Chaini26 – 985960Alpha-glucosidasePRO_0000018577Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi220 – 2201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi508 – 5081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi536 – 5361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi602 – 6021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi624 – 6241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi661 – 6611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi835 – 8351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi881 – 8811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi929 – 9291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi957 – 9571N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    By maltose.

    Interactioni

    Protein-protein interaction databases

    STRINGi5062.CADAORAP00001723.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12558.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1501.
    HOGENOMiHOG000041175.
    KOiK01187.
    OMAiYDYPEGF.
    OrthoDBiEOG77T1CZ.

    Family and domain databases

    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01055. Glyco_hydro_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 2 hits.
    PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12558-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGLKSFLAS SWLLPVACGA SQSIVPSTSA TAAYSQFTIP ASADVGANLV    50
    ANIDDPQAVN AQSVCPGYKA SDVKHSSQGF TASLELAGDP CNVYGTDVDS 100
    LTLTVEYQAK DRLNIQIVPT YFDASNASWY ILSEELVPRP KASQNASVPQ 150
    SDFVVSWSNE PSFNFKVIRK ATGDVLFNTK GSTLVYENQF IEFVTLLPEE 200
    YNLYGLGERM NQLRLLENAN LTLYAADIAD PIDDNIYGHH AFYLDTRYYK 250
    VGGQNKSHTI VKSSEAEPSQ EYVSYSHGVF LRNAHGQEIL LRDQKLIWRT 300
    LGGSVDLTFY SGPTQAEVTK QYQLSTVGLP AMQQYNTLGF HQCRWGYNNW 350
    SEFEDVLANF ERFEIPLEYL WADIDYMHGY RNFDNDQHRF SYEEGEKFLN 400
    KLHAGGRRWV PIVDGALYIP NPENASDAYE TYDRGAKDDV FIKNPDGSLY 450
    IGAVWPGYTV YPDWHHPKAS DFWANELVTW WNKLHYDGVW YDMAEVSSFC 500
    VGSCGTGNLS MNPAHPPFAL PGEPGNVVYD YPEGFNITNA TEAASASAGA 550
    ASQSAAASST TTSAPYLRTT PTPGVRNVDH PPYVINHVQP GHDLSVHAIS 600
    PNSTHSDGVQ EYDVHSLYGH QGINATYHGL LKVWENKRPF IIARSTFSGS 650
    GKWAGHWGGD NFSKWGSMFF SISQALQFSL FGIPMFGVDT CGFNGNTDEE 700
    LCNRWMQLSA FFPFYRNHNV LSAIPQEPYR WASVIDATKA AMNIRYAILP 750
    YFYTLFHLAH TTGSTVMRAL AWEFPNDPSL AAVGTQFLVG PSVMVIPVLE 800
    PQVDTVQGVF PGVGHGEVWY DWYSQTAVDA KPGVNTTISA PLGHIPVFVR 850
    GGSILPMQEV ALTTRDARKT PWSLLASLSS NGTASGQLYL DDGESVYPED 900
    TLSVDFLASR STLRASARGT WKEANPLANV TVLGVTEKPS SVTLNGETLS 950
    SDSVKYNATS HVLHVGGLQK HTADGAWAKD WVLKW 985
    Length:985
    Mass (Da):108,704
    Last modified:November 1, 1996 - v1
    Checksum:i3E9EAE0A0E38209E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45179 Genomic DNA. Translation: BAA08125.1.
    AB021876 Genomic DNA. Translation: BAA95702.1.
    AP007155 Genomic DNA. Translation: BAE58289.1.
    PIRiJC4217.
    RefSeqiXP_001820291.1. XM_001820239.2.

    Genome annotation databases

    EnsemblFungiiCADAORAT00001748; CADAORAP00001723; CADAORAG00001748.
    GeneIDi5992274.
    KEGGiaor:AOR_1_2146154.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45179 Genomic DNA. Translation: BAA08125.1 .
    AB021876 Genomic DNA. Translation: BAA95702.1 .
    AP007155 Genomic DNA. Translation: BAE58289.1 .
    PIRi JC4217.
    RefSeqi XP_001820291.1. XM_001820239.2.

    3D structure databases

    ProteinModelPortali Q12558.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5062.CADAORAP00001723.

    Protein family/group databases

    CAZyi GH31. Glycoside Hydrolase Family 31.
    mycoCLAPi AGL31A_ASPOR.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADAORAT00001748 ; CADAORAP00001723 ; CADAORAG00001748 .
    GeneIDi 5992274.
    KEGGi aor:AOR_1_2146154.

    Phylogenomic databases

    eggNOGi COG1501.
    HOGENOMi HOG000041175.
    KOi K01187.
    OMAi YDYPEGF.
    OrthoDBi EOG77T1CZ.

    Family and domain databases

    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 2 hits.
    PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and expression of alpha-glucosidase-encoding gene (agdA) from Aspergillus oryzae."
      Minetoki T., Gomi K., Kitamoto K., Kumagai C., Tamura G.
      Biosci. Biotechnol. Biochem. 59:1516-1521(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.
    2. "Molecular cloning and characterization of a transcriptional activator gene, amyR, involved in the amylolytic gene expression in Aspergillus oryzae."
      Gomi K., Akeno T., Minetoki T., Ozeki K., Kumagai C., Okazaki N., Iimura Y.
      Biosci. Biotechnol. Biochem. 64:816-827(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.
    3. "Genome sequencing and analysis of Aspergillus oryzae."
      Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.
      , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H.
      Nature 438:1157-1161(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 42149 / RIB 40.

    Entry informationi

    Entry nameiAGLU_ASPOR
    AccessioniPrimary (citable) accession number: Q12558
    Secondary accession number(s): Q7LWA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3