Reviewed, UniProtKB/Swiss-Prot Q12554 (PGLR3_ASPNG)

Names and origin

Protein names

Recommended name:
    Polygalacturonase-3
    EC=3.2.1.15
Alternative name(s):
    Polygalacturonase III
      Short name=PG-III
      Short name=PGC
    Pectinase-3

Gene names

Name:

pgaC

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 4 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 40	24	Potential		PRO_0000024772
	Chain	41 – 383	343	Polygalacturonase-3		PRO_0000024773
Sites
	Active site	221	1	Proton donor By similarity
	Active site	243	1	By similarity
Amino acid modifications
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 62		By similarity
	Disulfide bond	223 ↔ 239		By similarity
	Disulfide bond	348 ↔ 353		By similarity
	Disulfide bond	372 ↔ 381		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12554-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D6A4ED2149FC7A3A	FASTA	383	40,502
	10 20 30 40 50 60 MVRQLILISS LLAAVAVRAP ADPAHPMVTE APDVNLVEKR ATTCTFSGSE GASKASKSKT 70 80 90 100 110 120 SCSTIYLSDV AVPSGTTLDL SDLNDGTHVI FQGETTFGYE EWEGPLVRVS GTDITVEGES 130 140 150 160 170 180 DAVLNGDGSR WWDGEGGNGG KTKPKFFYAH DLTSSTIKSI YIENSPVQVF SIDGSTDLTM 190 200 210 220 230 240 TDITVDNTDG DTDDLAANTD GFDIGESTYI TITGAEIYNQ DDCVAINSGE NIYFSASVCS 250 260 270 280 290 300 GGHGLSIGSV GGRDDNTVKN VTFYDVNVLK SQQAIRIKTI YGDTGSVSEV TYHEIAFSDA 310 320 330 340 350 360 TDYGIVIEQN YDDTSKTPTT GVPITDFVLE NIVGTCEDDD CTEVYIACGD GSCSDWTWTG 370 380 VSVTGGSVSD DCLNVPSGIS CDL

References

[1]

"The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes." Bussink H.J.D., Buxton F.P., Fraaye B.A., de Graaff L.H., Visser J. Eur. J. Biochem. 208:83-90(1992) [PubMed: 1511691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64356 Genomic DNA. Translation: CAA45707.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 277.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR3_ASPNG

Accession

Primary (citable) accession number: Q12554

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)