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Q12553

- XDH_EMENI

UniProt

Q12553 - XDH_EMENI

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Protein

Xanthine dehydrogenase

Gene
hxA, AN5613
Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid By similarity.

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Binds 2 2Fe-2S clusters By similarity.
FAD By similarity.
Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 By similarity
Metal bindingi78 – 781Iron-sulfur 1 By similarity
Metal bindingi81 – 811Iron-sulfur 1 By similarity
Metal bindingi103 – 1031Iron-sulfur 1 By similarity
Metal bindingi142 – 1421Iron-sulfur 2 By similarity
Metal bindingi145 – 1451Iron-sulfur 2 By similarity
Metal bindingi177 – 1771Iron-sulfur 2 By similarity
Metal bindingi179 – 1791Iron-sulfur 2 By similarity
Binding sitei374 – 3741FAD By similarity
Binding sitei397 – 3971FAD By similarity
Binding sitei459 – 4591FAD By similarity
Metal bindingi798 – 7981Molybdenum By similarity
Metal bindingi829 – 8291Molybdenum; via carbonyl oxygen By similarity
Binding sitei833 – 8331Substrate By similarity
Binding sitei911 – 9111Substrate By similarity
Metal bindingi943 – 9431Molybdenum; via amide nitrogen By similarity
Binding sitei945 – 9451Substrate By similarity
Binding sitei1041 – 10411Substrate; via amide nitrogen By similarity
Metal bindingi1110 – 11101Molybdenum; via amide nitrogen By similarity
Active sitei1295 – 12951Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi294 – 3018FAD By similarity
Nucleotide bindingi384 – 3885FAD By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
  2. electron carrier activity Source: InterPro
  3. flavin adenine dinucleotide binding Source: UniProtKB
  4. iron ion binding Source: InterPro
  5. molybdopterin cofactor binding Source: UniProtKB
  6. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
  7. xanthine dehydrogenase activity Source: UniProtKB
  8. xanthine oxidase activity Source: InterPro

GO - Biological processi

  1. purine nucleobase catabolic process Source: ASPGD
  2. xanthine catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase (EC:1.17.1.4)
Alternative name(s):
Purine hydroxylase I
Gene namesi
Name:hxA
ORF Names:AN5613
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome V

Subcellular locationi

Peroxisome By similarity

GO - Cellular componenti

  1. peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13631363Xanthine dehydrogenasePRO_0000166087Add
BLAST

Expressioni

Inductioni

By 2-thiouric acid. Repressed by ammonium.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00003457.

Structurei

3D structure databases

ProteinModelPortaliQ12553.
SMRiQ12553. Positions 35-193.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 121872Fe-2S ferredoxin-typeAdd
BLAST
Domaini266 – 450185FAD-binding PCMH-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4630.
HOGENOMiHOG000191197.
KOiK00106.
OMAiCDETFFA.
OrthoDBiEOG76X67F.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12553-1 [UniParc]FASTAAdd to Basket

« Hide

MAPGVLLQPS QSELEAASPP KAAASLLQLT EEWDDTIRFY LNGTKVILDS     50
VDPEITLLEY LRGIGLTGTK LGCAEGGCGA CTVVVSQINP TTKKLYHASI 100
NACIAPLVAV DGKHVITVEG IGNVKNPHAI QQRLAIGNGS QCGFCTPGIV 150
MSLYALLRND PKPSEHAVEE AFDGNLCRCT GYRPILDAAQ SFTSPIGCGK 200
ARANGGSGCC MEEQKGTNGC CKGSSEETTE DVKHKFASPD FIEYKPDTEL 250
IFPPSLWKHE LRPLAFGNKR KKWYRPVTVQ QLLEIKSIHP DAKLIGGSTE 300
TQIEIKFKQM RYGASVYLGD LAELRQFAFH DNYLEIGANI SLTDLESVCD 350
QAIERYGSAR GQPFAAIKKQ LRYFAGRQIR NVASPAGNLA TASPISDLNP 400
VFVATNTTLV ARSLDKETEI PMTQFFRGYR STALPPDAII SSLRIPTASE 450
KGEYLRAYKQ SKRKDDDIAI VNAALRVSLS SSNDVTSVSL VFGGMAPLTV 500
SARNAEAFLT GKKFTDPATL EGTMGALEQD FNLKFGVPGG MATYRKSLAL 550
GFFYRFYHDV LSQIEARSSD LDNSVVAEIE RAISTGEKDN EASAAYQQRV 600
LGRAGPHLSA LKQATGEAQY TDDIPAQKNE LYGCMVLSTK AHAKLLSVNT 650
EAALEIPGVI DYVDHKDLPS PRANWWGAPN CDEVFFAVDK VTTAGQPIGM 700
ILANTAKAAE EGARAVKVEY EELPVILSIE EAIEAQSFFE RFRYIKNGDP 750
ESAFRDADHV FEGVSRMGGQ EHFYLETQAC VAIPKAEDGE MEIWSSTQNP 800
TETQSYVAQV TGVAANKIVS RVKRLGGGFG GKETRSVQLA GICATAAAKV 850
RRPVRCMLNR DEDIATSGQR HPFYCKWKVG VTREGKLLAL DADVYANGGH 900
TQDLSGAVVE RSLSHIDNVY RFPNIYVRGR ICKTNTVSNT AFRGFGGPQG 950
LFFAESIISE VADHLDLQVE QLRILNMYEP GDMTHFNQEL KDWHVPLMYD 1000
QVLQESEYFE RRKAVEEYNR THKWSKRGMA IIPTKFGISF TALFLNQAGA 1050
LVHIYHDGSV LVAHGGVEMG QGLHTKMTMI AAEALGVPLS DVFISETATN 1100
TVANTSSTAA SASSDLNGYA IYNACTQLNE RLKPYREKMP NATLKDLAHA 1150
AYFDRVNLSA QGYYRTPDIG YTWGENKGQM FFYFTQGVTA AEVEIDTLTG 1200
DWTPLRADIK MDVGRTINPS IDYGQIEGAY IQGQGLFTTE ESLWHRTTGQ 1250
IFTKGPGNYK IPGFRDIPQI FNVSLLKDVE WENLRTIQRS RGVGEPPLFM 1300
GSAAFFAIRD ALKAARKEWG VTDVLSLVSP ATPERIRVSC ADPIIERARV 1350
KAEEGEKSFF VAI 1363
Length:1,363
Mass (Da):149,523
Last modified:May 1, 2007 - v2
Checksum:i18D8F50B731EDE4B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti741 – 7411R → H in CAA58034. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82827 Genomic DNA. Translation: CAA58034.1.
AACD01000098 Genomic DNA. Translation: EAA62706.1.
BN001305 Genomic DNA. Translation: CBF81549.1.
PIRiA55875.
RefSeqiXP_663217.1. XM_658125.1.

Genome annotation databases

EnsemblFungiiCADANIAT00003457; CADANIAP00003457; CADANIAG00003457.
GeneIDi2871900.
KEGGiani:AN5613.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82827 Genomic DNA. Translation: CAA58034.1 .
AACD01000098 Genomic DNA. Translation: EAA62706.1 .
BN001305 Genomic DNA. Translation: CBF81549.1 .
PIRi A55875.
RefSeqi XP_663217.1. XM_658125.1.

3D structure databases

ProteinModelPortali Q12553.
SMRi Q12553. Positions 35-193.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 162425.CADANIAP00003457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00003457 ; CADANIAP00003457 ; CADANIAG00003457 .
GeneIDi 2871900.
KEGGi ani:AN5613.2.

Phylogenomic databases

eggNOGi COG4630.
HOGENOMi HOG000191197.
KOi K00106.
OMAi CDETFFA.
OrthoDBi EOG76X67F.

Family and domain databases

Gene3Di 1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProi IPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR014307. Xanthine_DH_ssu.
[Graphical view ]
Pfami PF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000127. Xanthine_DH. 1 hit.
SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view ]
SUPFAMi SSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsi TIGR02963. xanthine_xdhA. 1 hit.
PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and molecular characterization of hxA, the gene coding for the xanthine dehydrogenase (purine hydroxylase I) of Aspergillus nidulans."
    Glatigny A., Scazzocchio C.
    J. Biol. Chem. 270:3534-3550(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: biA1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiXDH_EMENI
AccessioniPrimary (citable) accession number: Q12553
Secondary accession number(s): C8VFY5, Q5B1G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi