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Protein

Acid phosphatase

Gene

aphA

Organism
Aspergillus ficuum
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Cu cationCurated

Enzyme regulationi

Competitively inhibited by phosphomycin and inorganic orthophosphate.

pH dependencei

Optimum pH is 6. Inactive above pH 6.7.

Temperature dependencei

Optimum temperature is 63 degrees Celsius.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Copper

Names & Taxonomyi

Protein namesi
Recommended name:
Acid phosphatase (EC:3.1.3.2)
Alternative name(s):
APase6
pH 6-optimum acid phosphatase
Gene namesi
Name:aphA
OrganismiAspergillus ficuum
Taxonomic identifieri5058 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 222 PublicationsAdd BLAST22
ChainiPRO_000002399723 – 605Acid phosphataseAdd BLAST583
PropeptideiPRO_0000023998606 – 6149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi242N-linked (GlcNAc...)Sequence analysis1
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi333N-linked (GlcNAc...)Sequence analysis1
Glycosylationi340N-linked (GlcNAc...)Sequence analysis1
Glycosylationi352N-linked (GlcNAc...)Sequence analysis1
Glycosylationi408N-linked (GlcNAc...)Sequence analysis1
Glycosylationi429N-linked (GlcNAc...)Sequence analysis1
Glycosylationi512N-linked (GlcNAc...)Sequence analysis1
Glycosylationi523N-linked (GlcNAc...)Sequence analysis1
Glycosylationi559N-linked (GlcNAc...)Sequence analysis1
Glycosylationi578N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated; probably with N-linked high-mannose oligosaccharides.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ12546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 176Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST97

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 3 hits.
InterProiIPR014390. Acid_Pase_Asper.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR003961. FN3_dom.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000900. Acid_Ptase_Asper. 1 hit.
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGTAASALL VALSATAAQA RPVVDERFPY TGPAVPIGDW VDPTINGNGK
60 70 80 90 100
GFPRLVEPPA VKPATANPRN NVNVISLSYI PKGMHIHYQT PFGLGQLPAV
110 120 130 140 150
RWGKDPRNLN STAQGYSHTY DRTPSCSQVK AVTQCSQFFH EVSIDGLEPD
160 170 180 190 200
TTYYYQIPAA NGTTQSEVLS FKTSRPAGHP GSFSVAVLND MGYTNAHGTH
210 220 230 240 250
KQLVKAATEG TAFAWHGGDL SYADDWYSGI LACADDWPVC YNGTSSTLPG
260 270 280 290 300
GGPLPEEYKK PLPAGEIPDQ GGPQGGDMSV LYESNWDLWQ QWLNNVTLKI
310 320 330 340 350
PYMVLPGNHE ASCAEFDGPH NILTAYLNDD IANGTAPTDN LTYYSCPPSQ
360 370 380 390 400
RNFTAYQHRF RMPGPETGGV GNFWYSFDYG LAHFVSIDGE TDFANSPEWN
410 420 430 440 450
FAEDVTGNET LPSESETFIT DSGPFGNVNG SVHETKSYEQ WHWLQQDLAK
460 470 480 490 500
VDRSKTPWVI VMSHRPMYSS AYSSYQLHVR EAFEGLLLKY GVDAYLSGHI
510 520 530 540 550
HWYERLYPLG ANGTIDTAAI VNNNTYYAHN GKSITHIING MAGNIESHSE
560 570 580 590 600
FSDGEGLTNI TALLDKVHYG FSKLTIFNET ALKWELIRGD DGTVGDSLTL
610
LKPSHVAGGK KLHS
Length:614
Mass (Da):67,211
Last modified:November 1, 1996 - v1
Checksum:i7016738F63C62EBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18553 Genomic DNA. Translation: AAA91632.1.
PIRiJC2545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18553 Genomic DNA. Translation: AAA91632.1.
PIRiJC2545.

3D structure databases

ProteinModelPortaliQ12546.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 3 hits.
InterProiIPR014390. Acid_Pase_Asper.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR003961. FN3_dom.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000900. Acid_Ptase_Asper. 1 hit.
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPA_ASPFI
AccessioniPrimary (citable) accession number: Q12546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.