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Q12546

- PPA_ASPFI

UniProt

Q12546 - PPA_ASPFI

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Protein

Acid phosphatase

Gene

aphA

Organism
Aspergillus ficuum
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Copper.Curated

Enzyme regulationi

Competitively inhibited by phosphomycin and inorganic orthophosphate.

pH dependencei

Optimum pH is 6. Inactive above pH 6.7.

Temperature dependencei

Optimum temperature is 63 degrees Celsius.

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Copper

Names & Taxonomyi

Protein namesi
Recommended name:
Acid phosphatase (EC:3.1.3.2)
Alternative name(s):
APase6
pH 6-optimum acid phosphatase
Gene namesi
Name:aphA
OrganismiAspergillus ficuum
Taxonomic identifieri5058 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22222 PublicationsAdd
BLAST
Chaini23 – 605583Acid phosphatasePRO_0000023997Add
BLAST
Propeptidei606 – 6149PRO_0000023998

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi512 – 5121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi559 – 5591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi578 – 5781N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated; probably with N-linked high-mannose oligosaccharides.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ12546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 17697Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 3 hits.
InterProiIPR014390. Acid_Pase_Asper.
IPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000900. Acid_Ptase_Asper. 1 hit.
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12546-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGTAASALL VALSATAAQA RPVVDERFPY TGPAVPIGDW VDPTINGNGK
60 70 80 90 100
GFPRLVEPPA VKPATANPRN NVNVISLSYI PKGMHIHYQT PFGLGQLPAV
110 120 130 140 150
RWGKDPRNLN STAQGYSHTY DRTPSCSQVK AVTQCSQFFH EVSIDGLEPD
160 170 180 190 200
TTYYYQIPAA NGTTQSEVLS FKTSRPAGHP GSFSVAVLND MGYTNAHGTH
210 220 230 240 250
KQLVKAATEG TAFAWHGGDL SYADDWYSGI LACADDWPVC YNGTSSTLPG
260 270 280 290 300
GGPLPEEYKK PLPAGEIPDQ GGPQGGDMSV LYESNWDLWQ QWLNNVTLKI
310 320 330 340 350
PYMVLPGNHE ASCAEFDGPH NILTAYLNDD IANGTAPTDN LTYYSCPPSQ
360 370 380 390 400
RNFTAYQHRF RMPGPETGGV GNFWYSFDYG LAHFVSIDGE TDFANSPEWN
410 420 430 440 450
FAEDVTGNET LPSESETFIT DSGPFGNVNG SVHETKSYEQ WHWLQQDLAK
460 470 480 490 500
VDRSKTPWVI VMSHRPMYSS AYSSYQLHVR EAFEGLLLKY GVDAYLSGHI
510 520 530 540 550
HWYERLYPLG ANGTIDTAAI VNNNTYYAHN GKSITHIING MAGNIESHSE
560 570 580 590 600
FSDGEGLTNI TALLDKVHYG FSKLTIFNET ALKWELIRGD DGTVGDSLTL
610
LKPSHVAGGK KLHS
Length:614
Mass (Da):67,211
Last modified:November 1, 1996 - v1
Checksum:i7016738F63C62EBE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18553 Genomic DNA. Translation: AAA91632.1.
PIRiJC2545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18553 Genomic DNA. Translation: AAA91632.1 .
PIRi JC2545.

3D structure databases

ProteinModelPortali Q12546.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 3 hits.
InterProi IPR014390. Acid_Pase_Asper.
IPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000900. Acid_Ptase_Asper. 1 hit.
SMARTi SM00060. FN3. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
PROSITEi PS50853. FN3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Aspergillus niger (ficuum) aphA gene encodes a pH 6.0-optimum acid phosphatase."
    Mullaney E.J., Daly C.B., Ehrlich K.C., Ullah A.H.J.
    Gene 162:117-121(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
  2. "The complete primary structure elucidation of Aspergillus ficuum (niger), pH 6.0, optimum acid phosphatase by Edman degradation."
    Ullah A.H.J., Mullaney E.M., Dischinger H.C. Jr.
    Biochem. Biophys. Res. Commun. 203:182-189(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-605.
    Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
  3. "Aspergillus ficuum extracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence, and biochemical characterization."
    Ullah A.H.J., Cummins B.J.
    Prep. Biochem. 18:37-65(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-56, CHARACTERIZATION.
    Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.

Entry informationi

Entry nameiPPA_ASPFI
AccessioniPrimary (citable) accession number: Q12546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3