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Q12546

- PPA_ASPFI

UniProt

Q12546 - PPA_ASPFI

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Protein
Acid phosphatase
Gene
aphA
Organism
Aspergillus ficuum
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Copper Reviewed prediction.

Enzyme regulationi

Competitively inhibited by phosphomycin and inorganic orthophosphate.

pH dependencei

Optimum pH is 6. Inactive above pH 6.7.

Temperature dependencei

Optimum temperature is 63 degrees Celsius.

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Copper

Names & Taxonomyi

Protein namesi
Recommended name:
Acid phosphatase (EC:3.1.3.2)
Alternative name(s):
APase6
pH 6-optimum acid phosphatase
Gene namesi
Name:aphA
OrganismiAspergillus ficuum
Taxonomic identifieri5058 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22222 Publications
Add
BLAST
Chaini23 – 605583Acid phosphatase
PRO_0000023997Add
BLAST
Propeptidei606 – 6149
PRO_0000023998

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi161 – 1611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi242 – 2421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi295 – 2951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi333 – 3331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi340 – 3401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi352 – 3521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi408 – 4081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi429 – 4291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi512 – 5121N-linked (GlcNAc...) Reviewed prediction
Glycosylationi523 – 5231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi559 – 5591N-linked (GlcNAc...) Reviewed prediction
Glycosylationi578 – 5781N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated; probably with N-linked high-mannose oligosaccharides.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ12546.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 17697Fibronectin type-III
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 3 hits.
InterProiIPR014390. Acid_Pase_Asper.
IPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000900. Acid_Ptase_Asper. 1 hit.
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12546-1 [UniParc]FASTAAdd to Basket

« Hide

MKGTAASALL VALSATAAQA RPVVDERFPY TGPAVPIGDW VDPTINGNGK    50
GFPRLVEPPA VKPATANPRN NVNVISLSYI PKGMHIHYQT PFGLGQLPAV 100
RWGKDPRNLN STAQGYSHTY DRTPSCSQVK AVTQCSQFFH EVSIDGLEPD 150
TTYYYQIPAA NGTTQSEVLS FKTSRPAGHP GSFSVAVLND MGYTNAHGTH 200
KQLVKAATEG TAFAWHGGDL SYADDWYSGI LACADDWPVC YNGTSSTLPG 250
GGPLPEEYKK PLPAGEIPDQ GGPQGGDMSV LYESNWDLWQ QWLNNVTLKI 300
PYMVLPGNHE ASCAEFDGPH NILTAYLNDD IANGTAPTDN LTYYSCPPSQ 350
RNFTAYQHRF RMPGPETGGV GNFWYSFDYG LAHFVSIDGE TDFANSPEWN 400
FAEDVTGNET LPSESETFIT DSGPFGNVNG SVHETKSYEQ WHWLQQDLAK 450
VDRSKTPWVI VMSHRPMYSS AYSSYQLHVR EAFEGLLLKY GVDAYLSGHI 500
HWYERLYPLG ANGTIDTAAI VNNNTYYAHN GKSITHIING MAGNIESHSE 550
FSDGEGLTNI TALLDKVHYG FSKLTIFNET ALKWELIRGD DGTVGDSLTL 600
LKPSHVAGGK KLHS 614
Length:614
Mass (Da):67,211
Last modified:November 1, 1996 - v1
Checksum:i7016738F63C62EBE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18553 Genomic DNA. Translation: AAA91632.1.
PIRiJC2545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18553 Genomic DNA. Translation: AAA91632.1 .
PIRi JC2545.

3D structure databases

ProteinModelPortali Q12546.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 3 hits.
InterProi IPR014390. Acid_Pase_Asper.
IPR004843. Calcineurin-like_PHP_apaH.
IPR003961. Fibronectin_type3.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000900. Acid_Ptase_Asper. 1 hit.
SMARTi SM00060. FN3. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
PROSITEi PS50853. FN3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Aspergillus niger (ficuum) aphA gene encodes a pH 6.0-optimum acid phosphatase."
    Mullaney E.J., Daly C.B., Ehrlich K.C., Ullah A.H.J.
    Gene 162:117-121(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
  2. "The complete primary structure elucidation of Aspergillus ficuum (niger), pH 6.0, optimum acid phosphatase by Edman degradation."
    Ullah A.H.J., Mullaney E.M., Dischinger H.C. Jr.
    Biochem. Biophys. Res. Commun. 203:182-189(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-605.
    Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
  3. "Aspergillus ficuum extracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence, and biochemical characterization."
    Ullah A.H.J., Cummins B.J.
    Prep. Biochem. 18:37-65(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-56, CHARACTERIZATION.
    Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.

Entry informationi

Entry nameiPPA_ASPFI
AccessioniPrimary (citable) accession number: Q12546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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