Acid phosphatase precursor - Aspergillus ficuum

Contribute

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q12546 (PPA_ASPFI)

Last modified June 16, 2009. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information

Names and origin

Protein names

Recommended name:
    Acid phosphatase
    EC=3.1.3.2
Alternative name(s):
    pH 6-optimum acid phosphatase
    APase6

Gene names

Name:

aphA

Organism

Aspergillus ficuum

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H₂O = an alcohol + phosphate.
Cofactor	Copper Potential.
Enzyme regulation	Competitively inhibited by phosphomycin and inorganic orthophosphate.
Subunit structure	Monomer.
Subcellular location	Secreted.
Post-translational modification	Glycosylated; probably with N-linked high-mannose oligosaccharides.
biophysicochemical properties	pH dependence: Optimum pH is 6. Inactive above pH 6.7. Temperature dependence: Optimum temperature is 63 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Copper
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

22

Chain

583

Acid phosphatase

PRO_0000023997

Propeptide

9

PRO_0000023998

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q12546-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7016738F63C62EBE
Show »

614

67,211

        10         20         30         40         50         60 
MKGTAASALL VALSATAAQA RPVVDERFPY TGPAVPIGDW VDPTINGNGK GFPRLVEPPA 

        70         80         90        100        110        120 
VKPATANPRN NVNVISLSYI PKGMHIHYQT PFGLGQLPAV RWGKDPRNLN STAQGYSHTY 

       130        140        150        160        170        180 
DRTPSCSQVK AVTQCSQFFH EVSIDGLEPD TTYYYQIPAA NGTTQSEVLS FKTSRPAGHP 

       190        200        210        220        230        240 
GSFSVAVLND MGYTNAHGTH KQLVKAATEG TAFAWHGGDL SYADDWYSGI LACADDWPVC 

       250        260        270        280        290        300 
YNGTSSTLPG GGPLPEEYKK PLPAGEIPDQ GGPQGGDMSV LYESNWDLWQ QWLNNVTLKI 

       310        320        330        340        350        360 
PYMVLPGNHE ASCAEFDGPH NILTAYLNDD IANGTAPTDN LTYYSCPPSQ RNFTAYQHRF 

       370        380        390        400        410        420 
RMPGPETGGV GNFWYSFDYG LAHFVSIDGE TDFANSPEWN FAEDVTGNET LPSESETFIT 

       430        440        450        460        470        480 
DSGPFGNVNG SVHETKSYEQ WHWLQQDLAK VDRSKTPWVI VMSHRPMYSS AYSSYQLHVR 

       490        500        510        520        530        540 
EAFEGLLLKY GVDAYLSGHI HWYERLYPLG ANGTIDTAAI VNNNTYYAHN GKSITHIING 

       550        560        570        580        590        600 
MAGNIESHSE FSDGEGLTNI TALLDKVHYG FSKLTIFNET ALKWELIRGD DGTVGDSLTL 

       610 
LKPSHVAGGK KLHS

References

[1]	"The Aspergillus niger (ficuum) aphA gene encodes a pH 6.0-optimum acid phosphatase." Mullaney E.J., Daly C.B., Ehrlich K.C., Ullah A.H.J. Gene 162:117-121(1995) [PubMed: 7557398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
[2]	"The complete primary structure elucidation of Aspergillus ficuum (niger), pH 6.0, optimum acid phosphatase by Edman degradation." Ullah A.H.J., Mullaney E.M., Dischinger H.C. Jr. Biochem. Biophys. Res. Commun. 203:182-189(1994) [PubMed: 8074654] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-605. Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
[3]	"Aspergillus ficuum extracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence, and biochemical characterization." Ullah A.H.J., Cummins B.J. Prep. Biochem. 18:37-65(1988) [PubMed: 3375203] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-56, CHARACTERIZATION. Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.

Cross-references

Sequence databases
	U18553 Genomic DNA. Translation: AAA91632.1.
PIR	JC2545.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.2. 81696.
Family and domain databases
InterPro	IPR014390. Acid_Pase_Asper. IPR003961. FN_III. IPR004843. M-pesterase. IPR015914. Purple_acid_Pase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000900. Acid_Ptase_Asper. 1 hit.
SMART	SM00060. FN3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA_ASPFI

Accession

Primary (citable) accession number: Q12546

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information