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Q12546 (PPA_ASPFI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acid phosphatase
EC=3.1.3.2
Alternative name(s):
APase6
pH 6-optimum acid phosphatase
Gene names
Name:aphA
OrganismAspergillus ficuum
Taxonomic identifier5058 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Copper Potential.

Enzyme regulation

Competitively inhibited by phosphomycin and inorganic orthophosphate.

Subunit structure

Monomer.

Subcellular location

Secreted.

Post-translational modification

Glycosylated; probably with N-linked high-mannose oligosaccharides.

Biophysicochemical properties

pH dependence:

Optimum pH is 6. Inactive above pH 6.7.

Temperature dependence:

Optimum temperature is 63 degrees Celsius.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCopper
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.2 Ref.3
Chain23 – 605583Acid phosphatase
PRO_0000023997
Propeptide606 – 6149
PRO_0000023998

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation2421N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3331N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Glycosylation5121N-linked (GlcNAc...) Potential
Glycosylation5231N-linked (GlcNAc...) Potential
Glycosylation5591N-linked (GlcNAc...) Potential
Glycosylation5781N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q12546 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7016738F63C62EBE

FASTA61467,211
        10         20         30         40         50         60 
MKGTAASALL VALSATAAQA RPVVDERFPY TGPAVPIGDW VDPTINGNGK GFPRLVEPPA 

        70         80         90        100        110        120 
VKPATANPRN NVNVISLSYI PKGMHIHYQT PFGLGQLPAV RWGKDPRNLN STAQGYSHTY 

       130        140        150        160        170        180 
DRTPSCSQVK AVTQCSQFFH EVSIDGLEPD TTYYYQIPAA NGTTQSEVLS FKTSRPAGHP 

       190        200        210        220        230        240 
GSFSVAVLND MGYTNAHGTH KQLVKAATEG TAFAWHGGDL SYADDWYSGI LACADDWPVC 

       250        260        270        280        290        300 
YNGTSSTLPG GGPLPEEYKK PLPAGEIPDQ GGPQGGDMSV LYESNWDLWQ QWLNNVTLKI 

       310        320        330        340        350        360 
PYMVLPGNHE ASCAEFDGPH NILTAYLNDD IANGTAPTDN LTYYSCPPSQ RNFTAYQHRF 

       370        380        390        400        410        420 
RMPGPETGGV GNFWYSFDYG LAHFVSIDGE TDFANSPEWN FAEDVTGNET LPSESETFIT 

       430        440        450        460        470        480 
DSGPFGNVNG SVHETKSYEQ WHWLQQDLAK VDRSKTPWVI VMSHRPMYSS AYSSYQLHVR 

       490        500        510        520        530        540 
EAFEGLLLKY GVDAYLSGHI HWYERLYPLG ANGTIDTAAI VNNNTYYAHN GKSITHIING 

       550        560        570        580        590        600 
MAGNIESHSE FSDGEGLTNI TALLDKVHYG FSKLTIFNET ALKWELIRGD DGTVGDSLTL 

       610 
LKPSHVAGGK KLHS

« Hide

References

[1]"The Aspergillus niger (ficuum) aphA gene encodes a pH 6.0-optimum acid phosphatase."
Mullaney E.J., Daly C.B., Ehrlich K.C., Ullah A.H.J.
Gene 162:117-121(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
[2]"The complete primary structure elucidation of Aspergillus ficuum (niger), pH 6.0, optimum acid phosphatase by Edman degradation."
Ullah A.H.J., Mullaney E.M., Dischinger H.C. Jr.
Biochem. Biophys. Res. Commun. 203:182-189(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-605.
Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
[3]"Aspergillus ficuum extracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence, and biochemical characterization."
Ullah A.H.J., Cummins B.J.
Prep. Biochem. 18:37-65(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-56, CHARACTERIZATION.
Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U18553 Genomic DNA. Translation: AAA91632.1.
PIRJC2545.

3D structure databases

ProteinModelPortalQ12546.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.380. 1 hit.
InterProIPR014390. Acid_Pase_Asper.
IPR003961. Fibronectin_type3.
IPR004843. Metallo_PEstase_dom.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
PIRSFPIRSF000900. Acid_Ptase_Asper. 1 hit.
SMARTSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMSSF49363. Purple_Pase_N. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA_ASPFI
AccessionPrimary (citable) accession number: Q12546
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info