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Q12546

- PPA_ASPFI

UniProt

Q12546 - PPA_ASPFI

Protein

Acid phosphatase

Gene

aphA

Organism
Aspergillus ficuum
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Copper.Curated

    Enzyme regulationi

    Competitively inhibited by phosphomycin and inorganic orthophosphate.

    pH dependencei

    Optimum pH is 6. Inactive above pH 6.7.

    Temperature dependencei

    Optimum temperature is 63 degrees Celsius.

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Copper

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acid phosphatase (EC:3.1.3.2)
    Alternative name(s):
    APase6
    pH 6-optimum acid phosphatase
    Gene namesi
    Name:aphA
    OrganismiAspergillus ficuum
    Taxonomic identifieri5058 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22222 PublicationsAdd
    BLAST
    Chaini23 – 605583Acid phosphatasePRO_0000023997Add
    BLAST
    Propeptidei606 – 6149PRO_0000023998

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi340 – 3401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi512 – 5121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi559 – 5591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi578 – 5781N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated; probably with N-linked high-mannose oligosaccharides.

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini80 – 17697Fibronectin type-IIIPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 3 hits.
    InterProiIPR014390. Acid_Pase_Asper.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR003961. Fibronectin_type3.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000900. Acid_Ptase_Asper. 1 hit.
    SMARTiSM00060. FN3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 3 hits.
    PROSITEiPS50853. FN3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12546-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGTAASALL VALSATAAQA RPVVDERFPY TGPAVPIGDW VDPTINGNGK    50
    GFPRLVEPPA VKPATANPRN NVNVISLSYI PKGMHIHYQT PFGLGQLPAV 100
    RWGKDPRNLN STAQGYSHTY DRTPSCSQVK AVTQCSQFFH EVSIDGLEPD 150
    TTYYYQIPAA NGTTQSEVLS FKTSRPAGHP GSFSVAVLND MGYTNAHGTH 200
    KQLVKAATEG TAFAWHGGDL SYADDWYSGI LACADDWPVC YNGTSSTLPG 250
    GGPLPEEYKK PLPAGEIPDQ GGPQGGDMSV LYESNWDLWQ QWLNNVTLKI 300
    PYMVLPGNHE ASCAEFDGPH NILTAYLNDD IANGTAPTDN LTYYSCPPSQ 350
    RNFTAYQHRF RMPGPETGGV GNFWYSFDYG LAHFVSIDGE TDFANSPEWN 400
    FAEDVTGNET LPSESETFIT DSGPFGNVNG SVHETKSYEQ WHWLQQDLAK 450
    VDRSKTPWVI VMSHRPMYSS AYSSYQLHVR EAFEGLLLKY GVDAYLSGHI 500
    HWYERLYPLG ANGTIDTAAI VNNNTYYAHN GKSITHIING MAGNIESHSE 550
    FSDGEGLTNI TALLDKVHYG FSKLTIFNET ALKWELIRGD DGTVGDSLTL 600
    LKPSHVAGGK KLHS 614
    Length:614
    Mass (Da):67,211
    Last modified:November 1, 1996 - v1
    Checksum:i7016738F63C62EBE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18553 Genomic DNA. Translation: AAA91632.1.
    PIRiJC2545.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18553 Genomic DNA. Translation: AAA91632.1 .
    PIRi JC2545.

    3D structure databases

    ProteinModelPortali Q12546.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 3 hits.
    InterProi IPR014390. Acid_Pase_Asper.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR003961. Fibronectin_type3.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000900. Acid_Ptase_Asper. 1 hit.
    SMARTi SM00060. FN3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 3 hits.
    PROSITEi PS50853. FN3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Aspergillus niger (ficuum) aphA gene encodes a pH 6.0-optimum acid phosphatase."
      Mullaney E.J., Daly C.B., Ehrlich K.C., Ullah A.H.J.
      Gene 162:117-121(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
    2. "The complete primary structure elucidation of Aspergillus ficuum (niger), pH 6.0, optimum acid phosphatase by Edman degradation."
      Ullah A.H.J., Mullaney E.M., Dischinger H.C. Jr.
      Biochem. Biophys. Res. Commun. 203:182-189(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-605.
      Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.
    3. "Aspergillus ficuum extracellular pH 6.0 optimum acid phosphatase: purification, N-terminal amino acid sequence, and biochemical characterization."
      Ullah A.H.J., Cummins B.J.
      Prep. Biochem. 18:37-65(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-56, CHARACTERIZATION.
      Strain: ATCC 66876 / DSM 932 / SRRC 265 / NRRL 3135.

    Entry informationi

    Entry nameiPPA_ASPFI
    AccessioniPrimary (citable) accession number: Q12546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3