Reviewed, UniProtKB/Swiss-Prot Q12545 (LEU3_CEPAC)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
      Short name=3-IPM-DH
      Short name=IMDH
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase

Gene names

Name:

LEU2

Organism

Cephalosporium acremonium (Acremonium chrysogenum)

Taxonomic identifier

5044 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Acremonium

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	3-isopropylmalate dehydrogenase		PRO_0000083607
Regions
	Nucleotide binding	79 – 90	12	NAD By similarity
	Nucleotide binding	294 – 306	13	NAD By similarity
Sites
	Metal binding	229	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Metal binding	258	1	Magnesium or manganese By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity
	Site	143	1	Important for catalysis By similarity
	Site	196	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12545-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 0F99ACD5251CCB43	FASTA	380	40,637
	10 20 30 40 50 60 MTTTYKILVL PGDHIGPEIM AEAIKVLTTI ETHRPNLHFN LTTDLVGGTS IDTHGVPITQ 70 80 90 100 110 120 SVLDAAKASD AVLFGSIGGP EWAGVHPTPE SGLLQLRQHL DAFANLRPCE FLVPSLVGAS 130 140 150 160 170 180 PIREHVVKGT RFIVVRENCG GAYFGEKKEE EDVASDLWVY TRPEIERLAR VSAAVARIMG 190 200 210 220 230 240 RSEDDNQAAT VWSADKANVL ASGRLWRRIT SDIFAKEFPD ITLQHQLADS MAMLMVRDPR 250 260 270 280 290 300 RFNGVIHTDN TFGDILSDIS GAITGTLGLM PSASLCGVPG EGHRSNGIYE PVHGSAPDIS 310 320 330 340 350 360 GKGLANPVAQ ILSVAMMLRY SMGLEKEATA VERAVVKVLD AKSEGGLEIR TGDLGGRATC 370 380 SQVGDAVCEV LGPLLQGKKA

References

[1]	Kimura H., Matumura S., Suzuki M., Sumino Y. Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M8650.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50665 Genomic DNA. Translation: BAA09319.1.
3D structure databases
HSSP	HSSP built from PDB template 2AYQ based on UniProtKB P12010.
ModBase	Search...
Family and domain databases
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CEPAC

Accession

Primary (citable) accession number: Q12545

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)