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Protein

Laccase-1

Gene

lcc1

Organism
Agaricus bisporus (White button mushroom)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products.By similarity

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi82 – 821Copper 1; type 2By similarity
Metal bindingi84 – 841Copper 2; type 3By similarity
Metal bindingi127 – 1271Copper 2; type 3By similarity
Metal bindingi129 – 1291Copper 3; type 3By similarity
Metal bindingi417 – 4171Copper 4; type 1By similarity
Metal bindingi420 – 4201Copper 1; type 2By similarity
Metal bindingi422 – 4221Copper 3; type 3By similarity
Metal bindingi470 – 4701Copper 3; type 3By similarity
Metal bindingi471 – 4711Copper 4; type 1By similarity
Metal bindingi472 – 4721Copper 2; type 3By similarity
Metal bindingi476 – 4761Copper 4; type 1By similarity

GO - Molecular functioni

  1. copper ion binding Source: InterPro
  2. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-1 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase 1
Diphenol oxidase 1
Laccase I
Urishiol oxidase 1
Gene namesi
Name:lcc1
OrganismiAgaricus bisporus (White button mushroom)
Taxonomic identifieri5341 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAgaricaceaeAgaricus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 520501Laccase-1PRO_0000002918Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi299 – 2991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ12541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 145125Plastocyanin-like 1Add
BLAST
Domaini157 – 305149Plastocyanin-like 2Add
BLAST
Domaini375 – 488114Plastocyanin-like 3Add
BLAST

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated
Contains 3 plastocyanin-like domains.Curated

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12541-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLSNALVLV AACISSVVAK TRTFDFDLVN TRLAPDGFER DTVVINGEFP
60 70 80 90 100
GTLIQVNKGD SVRIPLHNKL TSPTMRRSVS IHWHGFFQAR TSGQDGPSFV
110 120 130 140 150
NQCPQPPNTT FTYEFSVAEQ SGTFWYHSHL STQYCDGLRG AFIVYDPRDP
160 170 180 190 200
LRHLYDVDDE STVITLAEWY HILAPDATNE FFSSGIIPVQ DSGLINGKGR
210 220 230 240 250
FNGGPLTPFA VVNVRRGKRY RLRVIAISCR PFFTFSVDNH SLVFMEADGV
260 270 280 290 300
EHDPVEVQNV DIYAAQRVSV ILHANQPIDN YWIRAPMTGG NPDRNPNLNI
310 320 330 340 350
SLTLAILRYH GARHVEPTTV NVPGHKLLDQ EMHPIRQEGP GKLGDGPPDK
360 370 380 390 400
HITLNIAQPN APFFDINGIS YISPTVPVLL QILSGAKRPE DVLPSEQIFF
410 420 430 440 450
VPKNSLIEVN IPGEGAHPFH LHGHNFDVVL ASNDDTFNFK NPPRRDVYPI
460 470 480 490 500
NGGNTTFRFF TDNPGAWFLH CHIDWHLEAG LAIVFAEAPE DNVSGPQSQI
510 520
TPQDWLDLCP EYNAIEPEFQ
Length:520
Mass (Da):58,099
Last modified:November 1, 1996 - v1
Checksum:i943DB3F23297B891
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10664 Genomic DNA. Translation: AAC18877.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10664 Genomic DNA. Translation: AAC18877.1.

3D structure databases

ProteinModelPortaliQ12541.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.420. 3 hits.
InterProiIPR001117. Cu-oxidase.
IPR011706. Cu-oxidase_2.
IPR011707. Cu-oxidase_3.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
[Graphical view]
PfamiPF00394. Cu-oxidase. 1 hit.
PF07731. Cu-oxidase_2. 1 hit.
PF07732. Cu-oxidase_3. 1 hit.
[Graphical view]
SUPFAMiSSF49503. SSF49503. 3 hits.
PROSITEiPS00079. MULTICOPPER_OXIDASE1. 2 hits.
PS00080. MULTICOPPER_OXIDASE2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of two laccase genes in the cultivated mushroom Agaricus bisporus."
    Perry C.R., Smith M., Britnell C.H., Wood D.A., Thurston C.F.
    J. Gen. Microbiol. 139:1209-1218(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: D649.
    Tissue: Mycelium.

Entry informationi

Entry nameiLAC1_AGABI
AccessioniPrimary (citable) accession number: Q12541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.