Q12535 (PME_ASPAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectinesterase
Short name=PE
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase

Gene names

Name:

pme1

Organism

Aspergillus aculeatus

Taxonomic identifier

5053 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	By similarity
	Chain	18 – 331	314	Pectinesterase		PRO_0000023472
Sites
	Active site	162	1	Proton donor By similarity
	Active site	183	1	Nucleophile By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Site	161	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12535 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 1F1C81BF1E32174F	FASTA	331	35,681
	10 20 30 40 50 60 MVKSVLASAL FAVSALAASR TTAPSGAIVV AKSGGDYTTI GDAIDALSTS TTDTQTIFIE 70 80 90 100 110 120 EGTYDEQVYL PAMTGKVIIY GQTENTDSYA DNLVTITHAI SYEDAGESDD LTATFRNKAV 130 140 150 160 170 180 GSQVYNLNIA NTCGQACHQA LALSAWADQQ GYYGCNFTGY QDTLLAQTGN QLYINSYIEG 190 200 210 220 230 240 AVDFIFGQHA RAWFQNVDIR VVEGPTSASI TANGRSSETD TSYYVINKST VAAKEGDDVA 250 260 270 280 290 300 EGTYYLGRPW SEYARVVFQQ TSMTNVINSL GWTEWSTSTP NTEYVTFGEY ANTGAGSEGT 310 320 330 RASFAEKLDA KLTITDILGS DYTSWVDTSY F

References

[1]	"Pectin methyl esterase from Aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme." Christgau S., Kofod L.V., Halkier T., Andersen L.N., Hockauf M., Dorreich K., Dalboege H., Kauppinen S. Biochem. J. 319:705-712(1996) [PubMed: 8920970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: KSM 510.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49378 mRNA. Translation: AAB42153.1.
3D structure databases
ProteinModelPortal	Q12535.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. 1 hit. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME_ASPAC

Accession

Primary (citable) accession number: Q12535

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 21, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program