Q12527 (ATG11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 11

Alternative name(s):
Cytoplasm to vacuole targeting protein 9

Gene names

Name:	ATG11
Synonyms:	CVT9
Ordered Locus Names:	YPR049C
ORF Names:	YP9499.07c

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cytoplasm to vacuole transport (Cvt) and pexophagy. recruits the ATG19-prAPE1 complex to the pre-autophagosome, the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion. recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation, through its interaction with ATG32. Ref.3 Ref.4 Ref.5 Ref.6 Ref.11 Ref.12
Subunit structure	Homodimer and potential homooligomers. Interacts with ATG1 kinase and the ATG19 and ATG34 cargo protein transporters. Interacts with ATG32. Ref.4 Ref.6 Ref.11 Ref.12 Ref.13
Subcellular location	Membrane; Peripheral membrane protein. Note: Punctate structures. Ref.5 Ref.8
Miscellaneous	Present with 86 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the ATG11 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CVT pathway Inferred from mutant phenotype. Source: SGD mitochondrion degradation Inferred from mutant phenotype. Source: SGD peroxisome degradation Inferred from mutant phenotype Ref.5. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD protein localization to pre-autophagosomal structure Inferred from mutant phenotype. Source: SGD
Cellular component	extrinsic to membrane Inferred from direct assay Ref.5. Source: SGD pre-autophagosomal structure Inferred from direct assay. Source: SGD
Molecular function	protein complex scaffold Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG1	P53104	2	EBI-31977,EBI-2657

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1178	1178	Autophagy-related protein 11		PRO_0000124554
Regions
	Coiled coil	538 – 572	35	Potential
	Coiled coil	696 – 850	155	Potential
	Compositional bias	598 – 610	13	Asn-rich
	Compositional bias	1050 – 1099	50	Ser-rich
Amino acid modifications
	Modified residue	613	1	Phosphoserine Ref.10
	Modified residue	935	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q12527 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A904B92E9AE79449	FASTA	1,178	135,025
	10 20 30 40 50 60 MADADEYSTA PTQQEITPLQ TTATIINAIS GECITTNVDF FVSLDKFKQF IARKWKIPPD 70 80 90 100 110 120 QLLILLPYGN KLKPSMFKEL LINRSFTLND FYVYDRRLFS LVSKPTPTNL LTSKDSNPMN 130 140 150 160 170 180 SPNSNDLTET LEYLIKNSHI SQYQGSDTIM IKPMPSPLED ADVDLSRLNY HSVTSLLTTN 190 200 210 220 230 240 LGWLSALEID VHYFKSLIPD IIAHIKRIFD GLTVCSQYLK LYCFDVESLY NSNVQFLNQL 250 260 270 280 290 300 VDNGMTSKWE KCFNDTLSKL TALEGDSLQK FINIESLLEN EKSVKILNHS INGKLNKIKR 310 320 330 340 350 360 EIDENASFRD IITVNIDRLR QMFTPNESKF ELEDQMAESF EVLVSEMRTR SRNVLDKEEE 370 380 390 400 410 420 EFNSQEFLKS MNVMLEKDKK ESVKTLFTIS QALYSQIGEL IDLKKSLQKH AVAILGNIAF 430 440 450 460 470 480 TQMEILGIKR LLLNECNKDL ELYKKYEVEF AQVEDLPLIY GLYLIEKYRR LSWFQQILSF 490 500 510 520 530 540 ISNFNQDLEL FKQNELRTRN KWVKNFGSIA TVFCEDLLSS SDFKRLNEYH SHTSPPNEDE 550 560 570 580 590 600 EDENENSIAN YRQDLVKVSQ AIDNYMTQIK ETDVSEPIID LLSKTLFETK RFHIIYSNFK 610 620 630 640 650 660 NNNNNSSNGN SISPEGSIAL KSDDVVKGYK TRIKKLESLL HEFQYSDIGH WPQGVLNTHL 670 680 690 700 710 720 KPFRGSATSI NKKKFLGASV LLEPANISEV NIDSVSQANN HQIQELESNV DDLLHQLQLL 730 740 750 760 770 780 KEENNRKSMQ ISEMGKKISD LEVEKTAYRE TLTNLNQELA RLTNEEQSHR TEIFTLNASF 790 800 810 820 830 840 KKQLNDIISQ DNEKIEKLTG DYDDVSKSRE RLQMDLDESN KKHEQEVNLL KADIERLGKQ 850 860 870 880 890 900 IVTSEKSYAE TNSSSMEKGE KFETIPLAED PGRENQISAY TQTLQDRIFD IISTNIFILE 910 920 930 940 950 960 NIGLLLTFDN NNNIQIRRVK GLKKGTAQSN ILDESTQMLD AHDNSLIKSP VFQKLKDEYE 970 980 990 1000 1010 1020 LIKSVANGSE KDTQQSIFLG NITQLYDNKL YEVAVIRRFK DIETLAKKLT KENKIKRTLL 1030 1040 1050 1060 1070 1080 ERFQREKVTL RNFQIGDLAL FLPTRENVNS VGSMSSSTSS LSSSFSSVDL STPPPLDAMS 1090 1100 1110 1120 1130 1140 IQSSPSVIHS NVINQASISG RDKNKLMRPW AAFTAFEEST RYFLKDEKGL TKGKEWFVGR 1150 1160 1170 IVTLEHFVAD SPSNNPFRLP KGSVWFQVTA VVVSYQGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway." Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J. J. Biol. Chem. 271:17621-17624(1996) [PubMed: 8663607] [Abstract] Cited for: FUNCTION.
[4]	"Tor-mediated induction of autophagy via an Apg1 protein kinase complex." Kamada Y., Funakoshi T., Shintani T., Nagano K., Ohsumi M., Ohsumi Y. J. Cell Biol. 150:1507-1513(2000) [PubMed: 10995454] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG1.
[5]	"Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole." Kim J., Kamada Y., Stromhaug P.E., Guan J., Hefner-Gravink A., Baba M., Scott S.V., Ohsumi Y., Dunn W.A. Jr., Klionsky D.J. J. Cell Biol. 153:381-396(2001) [PubMed: 11309418] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway." Shintani T., Huang W.-P., Stromhaug P.E., Klionsky D.J. Dev. Cell 3:825-837(2002) [PubMed: 12479808] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG19.
[7]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-613 AND SER-935, MASS SPECTROMETRY.
[11]	"Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy." Okamoto K., Kondo-Okamoto N., Ohsumi Y. Dev. Cell 17:87-97(2009) [PubMed: 19619494] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG32.
[12]	"Atg32 is a mitochondrial protein that confers selectivity during mitophagy." Kanki T., Wang K., Cao Y., Baba M., Klionsky D.J. Dev. Cell 17:98-109(2009) [PubMed: 19619495] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG32.
[13]	"Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p." Suzuki K., Kondo C., Morimoto M., Ohsumi Y. J. Biol. Chem. 285:30019-30025(2010) [PubMed: 20639194] [Abstract] Cited for: INTERACTION WITH ATG34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71255 Genomic DNA. Translation: CAA94996.1. Z49219 Genomic DNA. Translation: CAA89169.1. BK006949 Genomic DNA. Translation: DAA11473.1.
PIR	S54073.
RefSeq	NP_015374.1. NM_001184146.1.
3D structure databases
ProteinModelPortal	Q12527.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1492N.
IntAct	Q12527. 19 interactions.
MINT	MINT-397248.
STRING	Q12527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR049C; YPR049C; YPR049C.
GeneID	856162.
KEGG	sce:YPR049C.
NMPDR	fig|4932.3.peg.6510.
Organism-specific databases
CYGD	YPR049c.
SGD	S000006253. ATG11.
Phylogenomic databases
eggNOG	fuNOG05879.
GeneTree	EFGT00050000000484.
HOGENOM	HBG203964.
OMA	YGLYLIE.
OrthoDB	EOG41ZJKB.
Gene expression databases
ArrayExpress	Q12527.
Genevestigator	Q12527.
GermOnline	YPR049C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019460. Autophagy-rel_p11. [Graphical view]
KO	K08330.
Pfam	PF10377. ATG11. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	981307.

Entry information

Entry name

ATG11_YEAST

Accession

Primary (citable) accession number: Q12527
Secondary accession number(s): D6W457

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program