Reviewed, UniProtKB/Swiss-Prot Q12527 (ATG11_YEAST)

Names and origin

Protein names

Recommended name:
    Autophagy-related protein 11
Alternative name(s):
    Cytoplasm to vacuole targeting protein 9

Gene names

Name:	ATG11
Synonyms:	CVT9
Ordered Locus Names:	YPR049C
ORF Names:	YP9499.07c

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cytoplasm to vacuole transport (Cvt) and pexophagy. Recruites the ATG19-prAPE1 complex to the pre-autophagosome, the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion. Ref.2 Ref.3 Ref.4 Ref.5
Subunit structure	Homodimer and potential homooligomers. Interacts with ATG1 kinase and the ATG19 cargo protein transporter. Ref.3 Ref.5
Subcellular location	Membrane; Peripheral membrane protein. Note: Punctate structures. Ref.4 Ref.7
Miscellaneous	Present with 86 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ATG11 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Membrane
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	CVT pathway Inferred from genetic interaction. Source: SGD peroxisome degradation Ref.4 Inferred from mutant phenotype. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD protein localization to pre-autophagosomal structure Inferred from mutant phenotype. Source: SGD
Cellular component	extrinsic to membrane Ref.4 Inferred from direct assay. Source: SGD pre-autophagosomal structure Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Ref.4 Inferred from physical interaction. Source: IntAct protein complex scaffold Inferred from genetic interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-31977,EBI-31977
ATG1	P53104	1	EBI-31977,EBI-2657

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1178	1178	Autophagy-related protein 11		PRO_0000124554
Regions
	Coiled coil	538 – 572	35	Potential
	Coiled coil	696 – 850	155	Potential
	Compositional bias	598 – 610	13	Asn-rich
	Compositional bias	1050 – 1099	50	Ser-rich
Amino acid modifications
	Modified residue	613	1	Phosphoserine Ref.9
	Modified residue	935	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q12527-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A904B92E9AE79449	FASTA	1,178	135,025
	10 20 30 40 50 60 MADADEYSTA PTQQEITPLQ TTATIINAIS GECITTNVDF FVSLDKFKQF IARKWKIPPD 70 80 90 100 110 120 QLLILLPYGN KLKPSMFKEL LINRSFTLND FYVYDRRLFS LVSKPTPTNL LTSKDSNPMN 130 140 150 160 170 180 SPNSNDLTET LEYLIKNSHI SQYQGSDTIM IKPMPSPLED ADVDLSRLNY HSVTSLLTTN 190 200 210 220 230 240 LGWLSALEID VHYFKSLIPD IIAHIKRIFD GLTVCSQYLK LYCFDVESLY NSNVQFLNQL 250 260 270 280 290 300 VDNGMTSKWE KCFNDTLSKL TALEGDSLQK FINIESLLEN EKSVKILNHS INGKLNKIKR 310 320 330 340 350 360 EIDENASFRD IITVNIDRLR QMFTPNESKF ELEDQMAESF EVLVSEMRTR SRNVLDKEEE 370 380 390 400 410 420 EFNSQEFLKS MNVMLEKDKK ESVKTLFTIS QALYSQIGEL IDLKKSLQKH AVAILGNIAF 430 440 450 460 470 480 TQMEILGIKR LLLNECNKDL ELYKKYEVEF AQVEDLPLIY GLYLIEKYRR LSWFQQILSF 490 500 510 520 530 540 ISNFNQDLEL FKQNELRTRN KWVKNFGSIA TVFCEDLLSS SDFKRLNEYH SHTSPPNEDE 550 560 570 580 590 600 EDENENSIAN YRQDLVKVSQ AIDNYMTQIK ETDVSEPIID LLSKTLFETK RFHIIYSNFK 610 620 630 640 650 660 NNNNNSSNGN SISPEGSIAL KSDDVVKGYK TRIKKLESLL HEFQYSDIGH WPQGVLNTHL 670 680 690 700 710 720 KPFRGSATSI NKKKFLGASV LLEPANISEV NIDSVSQANN HQIQELESNV DDLLHQLQLL 730 740 750 760 770 780 KEENNRKSMQ ISEMGKKISD LEVEKTAYRE TLTNLNQELA RLTNEEQSHR TEIFTLNASF 790 800 810 820 830 840 KKQLNDIISQ DNEKIEKLTG DYDDVSKSRE RLQMDLDESN KKHEQEVNLL KADIERLGKQ 850 860 870 880 890 900 IVTSEKSYAE TNSSSMEKGE KFETIPLAED PGRENQISAY TQTLQDRIFD IISTNIFILE 910 920 930 940 950 960 NIGLLLTFDN NNNIQIRRVK GLKKGTAQSN ILDESTQMLD AHDNSLIKSP VFQKLKDEYE 970 980 990 1000 1010 1020 LIKSVANGSE KDTQQSIFLG NITQLYDNKL YEVAVIRRFK DIETLAKKLT KENKIKRTLL 1030 1040 1050 1060 1070 1080 ERFQREKVTL RNFQIGDLAL FLPTRENVNS VGSMSSSTSS LSSSFSSVDL STPPPLDAMS 1090 1100 1110 1120 1130 1140 IQSSPSVIHS NVINQASISG RDKNKLMRPW AAFTAFEEST RYFLKDEKGL TKGKEWFVGR 1150 1160 1170 IVTLEHFVAD SPSNNPFRLP KGSVWFQVTA VVVSYQGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway." Harding T.M., Hefner-Gravink A., Thumm M., Klionsky D.J. J. Biol. Chem. 271:17621-17624(1996) [PubMed: 8663607] [Abstract] Cited for: FUNCTION.
[3]	"Tor-mediated induction of autophagy via an Apg1 protein kinase complex." Kamada Y., Funakoshi T., Shintani T., Nagano K., Ohsumi M., Ohsumi Y. J. Cell Biol. 150:1507-1513(2000) [PubMed: 10995454] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG1.
[4]	"Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole." Kim J., Kamada Y., Stromhaug P.E., Guan J., Hefner-Gravink A., Baba M., Scott S.V., Ohsumi Y., Dunn W.A. Jr., Klionsky D.J. J. Cell Biol. 153:381-396(2001) [PubMed: 11309418] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Mechanism of cargo selection in the cytoplasm to vacuole targeting pathway." Shintani T., Huang W.-P., Stromhaug P.E., Klionsky D.J. Dev. Cell 3:825-837(2002) [PubMed: 12479808] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG19.
[6]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-613 AND SER-935, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71255 Genomic DNA. Translation: CAA94996.1. Z49219 Genomic DNA. Translation: CAA89169.1.
PIR	S54073.
RefSeq	NP_015374.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1492N.
IntAct	Q12527. 16 interactions.
Proteomic databases
PRIDE	Q12527.
Genome annotation databases
Ensembl	YPR049C. Saccharomyces cerevisiae. [Contig view]
GeneID	856162.
GenomeReviews	Gene locus YPR049C in contig U00094_GR.
KEGG	sce:YPR049C.
NMPDR	fig|4932.3.peg.6510.
Organism-specific databases
CYGD	YPR049c.
SGD	S000006253. ATG11.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12527.
OMA	Q12527. YGLYLIE.
Gene expression databases
ArrayExpress	Q12527.
GermOnline	YPR049C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019460. Autophagy-rel_prot_11. [Graphical view]
Pfam	PF10377. ATG11. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981307.

Entry information

Entry name

ATG11_YEAST

Accession

Primary (citable) accession number: Q12527

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)