Q12525 (MHT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homocysteine S-methyltransferase 1
EC=2.1.1.10

Alternative name(s):
S-methylmethionine:homocysteine methyltransferase 1
Short name=SMM:Hcy S-methyltransferase 1

Gene names

Name:	MHT1
Ordered Locus Names:	YLL062C
ORF Names:	L0552

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Homocysteine S-methyltransferase involved in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio. Converts also S-methylmethionine (SMM) to methionine. Ref.5
Catalytic activity	S-methyl-L-methionine + L-homocysteine = 2 L-methionine.
Cofactor	Zinc Potential.
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 606 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.6. Source: SGD
Molecular function	homocysteine S-methyltransferase activity Inferred from direct assay Ref.5. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TGL5	Q12043	1	EBI-37133,EBI-34845

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Homocysteine S-methyltransferase 1		PRO_0000114618
Regions
	Domain	6 – 320	315	Hcy-binding
Sites
	Metal binding	238	1	Zinc Potential
	Metal binding	305	1	Zinc Potential
	Metal binding	306	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12525 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A77194694B6E5C14	FASTA	324	36,715
	10 20 30 40 50 60 MKRIPIKELI VEHPGKVLIL DGGQGTELEN RGININSPVW SAAPFTSESF WEPSSQERKV 70 80 90 100 110 120 VEEMYRDFMI AGANILMTIT YQANFQSISE NTSIKTLAAY KRFLDKIVSF TREFIGEERY 130 140 150 160 170 180 LIGSIGPWAA HVSCEYTGDY GPHPENIDYY GFFKPQLENF NQNRDIDLIG FETIPNFHEL 190 200 210 220 230 240 KAILSWDEDI ISKPFYIGLS VDDNSLLRDG TTLEEISVHI KGLGNKINKN LLLMGVNCVS 250 260 270 280 290 300 FNQSALILKM LHEHLPGMPL LVYPNSGEIY NPKEKTWHRP TNKLDDWETT VKKFVDNGAR 310 320 IIGGCCRTSP KDIAEIASAV DKYS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 37 kb DNA fragment from chromosome XII of Saccharomyces cerevisiae including the subtelomeric region of the left arm." Wedler H., Wambutt R. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Reverse methionine biosynthesis from S-adenosylmethionine in eukaryotic cells." Thomas D., Becker A., Surdin-Kerjan Y. J. Biol. Chem. 275:40718-40724(2000) [PubMed: 11013242] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z47973 Genomic DNA. Translation: CAA87995.1. Z73167 Genomic DNA. Translation: CAA97515.1. AY558194 Genomic DNA. Translation: AAS56520.1. BK006945 Genomic DNA. Translation: DAA09263.1.
PIR	S50958.
RefSeq	NP_013038.1. NM_001181882.1.
3D structure databases
ProteinModelPortal	Q12525.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1934N.
IntAct	Q12525. 3 interactions.
MINT	MINT-397842.
STRING	Q12525.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLL062C; YLL062C; YLL062C.
GeneID	850664.
KEGG	sce:YLL062C.
NMPDR	fig|4932.3.peg.4029.
Organism-specific databases
CYGD	YLL062c.
SGD	S000003985. MHT1.
Phylogenomic databases
eggNOG	fuNOG11566.
GeneTree	EFGT00050000004751.
HOGENOM	HBG204042.
OMA	NTINAIY.
OrthoDB	EOG4MSH79.
Gene expression databases
ArrayExpress	Q12525.
Genevestigator	Q12525.
GermOnline	YLL062C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
SUPFAM	SSF82282. S_methyl_trans. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966638.

Entry information

Entry name

MHT1_YEAST

Accession

Primary (citable) accession number: Q12525
Secondary accession number(s): D6VXU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program