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Protein

Eukaryotic translation initiation factor 6

Gene

TIF6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by the GTPase RIA1/EFL1 and by SDO1. Also required for pre-rRNA processing.UniRule annotation6 Publications

Miscellaneous

Present with 18600 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: SGD
  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • mature ribosome assembly Source: InterPro
  • ribosomal large subunit biogenesis Source: SGD
  • ribosomal subunit export from nucleus Source: SGD
  • rRNA processing Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis, Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34176-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 6UniRule annotation
Short name:
eIF-6UniRule annotation
Gene namesi
Name:TIF6UniRule annotation
Synonyms:CDC95
Ordered Locus Names:YPR016C
ORF Names:LPZ15C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR016C
SGDiS000006220 TIF6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001537421 – 245Eukaryotic translation initiation factor 6Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174Phosphoserine; by CK1UniRule annotation1
Modified residuei175Phosphoserine; by CK1UniRule annotation1
Modified residuei231PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.UniRule annotation2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12522
PaxDbiQ12522
PRIDEiQ12522

PTM databases

iPTMnetiQ12522

Interactioni

Subunit structurei

Monomer. Associates with the 60S ribosomal subunit.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36193, 585 interactors
DIPiDIP-5395N
IntActiQ12522, 88 interactors
MINTiQ12522
STRINGi4932.YPR016C

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi13 – 16Combined sources4
Beta strandi17 – 19Combined sources3
Beta strandi24 – 27Combined sources4
Helixi32 – 42Combined sources11
Turni43 – 45Combined sources3
Beta strandi48 – 51Combined sources4
Helixi59 – 62Combined sources4
Beta strandi67 – 73Combined sources7
Helixi78 – 87Combined sources10
Beta strandi92 – 97Combined sources6
Helixi104 – 107Combined sources4
Beta strandi108 – 110Combined sources3
Beta strandi112 – 117Combined sources6
Helixi123 – 133Combined sources11
Beta strandi135 – 139Combined sources5
Helixi148 – 150Combined sources3
Beta strandi151 – 154Combined sources4
Beta strandi159 – 161Combined sources3
Helixi167 – 177Combined sources11
Beta strandi181 – 183Combined sources3
Turni187 – 189Combined sources3
Helixi193 – 196Combined sources4
Beta strandi197 – 199Combined sources3
Beta strandi204 – 207Combined sources4
Helixi212 – 221Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G62X-ray2.50A1-224[»]
2X7Nelectron microscopy11.80B1-224[»]
3J2Ielectron microscopy11.90B1-245[»]
3JCTelectron microscopy3.08y1-245[»]
4V7Felectron microscopy8.70m1-245[»]
5FL8electron microscopy9.50m1-245[»]
5H4Pelectron microscopy3.07y1-227[»]
5JCSelectron microscopy9.50m1-245[»]
5T62electron microscopy3.30X1-245[»]
5Z3Gelectron microscopy3.65N1-245[»]
6C0Felectron microscopy3.70y1-245[»]
6ELZelectron microscopy3.30y1-245[»]
6EM1electron microscopy3.60y1-245[»]
6EM4electron microscopy4.10y1-245[»]
6EM5electron microscopy4.30y1-245[»]
6FT6electron microscopy3.90y1-245[»]
ProteinModelPortaliQ12522
SMRiQ12522
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12522

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-6 family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000015972
HOGENOMiHOG000230605
InParanoidiQ12522
KOiK03264
OMAiNDWCAFT
OrthoDBiEOG092C4BDP

Family and domain databases

CDDicd00527 IF6, 1 hit
HAMAPiMF_00032 eIF_6, 1 hit
InterProiView protein in InterPro
IPR002769 eIF6
PANTHERiPTHR10784 PTHR10784, 1 hit
PfamiView protein in Pfam
PF01912 eIF-6, 1 hit
PIRSFiPIRSF006413 IF-6, 1 hit
SMARTiView protein in SMART
SM00654 eIF6, 1 hit
TIGRFAMsiTIGR00323 eIF-6, 1 hit

Sequencei

Sequence statusi: Complete.

Q12522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH
60 70 80 90 100
TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLPD SVKIQRVEER
110 120 130 140 150
LSALGNVICC NDYVALVHPD IDRETEELIS DVLGVEVFRQ TISGNILVGS
160 170 180 190 200
YCSLSNQGGL VHPQTSVQDQ EELSSLLQVP LVAGTVNRGS SVVGAGMVVN
210 220 230 240
DYLAVTGLDT TAPELSVIES IFRLQDAQPE SISGNLRDTL IETYS
Length:245
Mass (Da):26,458
Last modified:November 1, 1996 - v1
Checksum:i1BCE041C61DFD2A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA Translation: CAA95012.1
Z49919 Genomic DNA Translation: CAA90161.1
U31900 Genomic DNA Translation: AAA97594.1
AY692916 Genomic DNA Translation: AAT92935.1
BK006949 Genomic DNA Translation: DAA11442.1
PIRiS57550
RefSeqiNP_015341.1, NM_001184113.1

Genome annotation databases

EnsemblFungiiYPR016C; YPR016C; YPR016C
GeneIDi856126
KEGGisce:YPR016C

Similar proteinsi

Entry informationi

Entry nameiIF6_YEAST
AccessioniPrimary (citable) accession number: Q12522
Secondary accession number(s): D6W426
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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