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Protein

Eukaryotic translation initiation factor 6

Gene

TIF6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by the GTPase RIA1/EFL1 and by SDO1. Also required for pre-rRNA processing.UniRule annotation6 Publications

GO - Molecular functioni

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: SGD
  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • mature ribosome assembly Source: UniProtKB-HAMAP
  • ribosomal large subunit biogenesis Source: SGD
  • ribosomal subunit export from nucleus Source: SGD
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-34176-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 6UniRule annotation
Short name:
eIF-6UniRule annotation
Gene namesi
Name:TIF6UniRule annotation
Synonyms:CDC95
Ordered Locus Names:YPR016C
ORF Names:LPZ15C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR016C.
SGDiS000006220. TIF6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: GO_Central
  • nucleolus Source: SGD
  • nucleus Source: SGD
  • preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001537421 – 245Eukaryotic translation initiation factor 6Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174Phosphoserine; by CK1UniRule annotation1
Modified residuei175Phosphoserine; by CK1UniRule annotation1
Modified residuei231PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.UniRule annotation2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12522.
PRIDEiQ12522.

PTM databases

iPTMnetiQ12522.

Interactioni

Subunit structurei

Monomer. Associates with the 60S ribosomal subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-9046,EBI-9046
LSG1P531455EBI-9046,EBI-23885
NOG2P537424EBI-9046,EBI-28532

Protein-protein interaction databases

BioGridi36193. 133 interactors.
DIPiDIP-5395N.
IntActiQ12522. 77 interactors.
MINTiMINT-527536.

Structurei

Secondary structure

1245
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi13 – 16Combined sources4
Beta strandi17 – 19Combined sources3
Beta strandi24 – 27Combined sources4
Helixi32 – 42Combined sources11
Turni43 – 45Combined sources3
Beta strandi48 – 51Combined sources4
Helixi59 – 62Combined sources4
Beta strandi67 – 73Combined sources7
Helixi78 – 87Combined sources10
Beta strandi92 – 97Combined sources6
Helixi104 – 107Combined sources4
Beta strandi108 – 110Combined sources3
Beta strandi112 – 117Combined sources6
Helixi123 – 133Combined sources11
Beta strandi135 – 139Combined sources5
Helixi148 – 150Combined sources3
Beta strandi151 – 154Combined sources4
Beta strandi159 – 161Combined sources3
Helixi167 – 177Combined sources11
Beta strandi181 – 183Combined sources3
Turni187 – 189Combined sources3
Helixi193 – 196Combined sources4
Beta strandi197 – 199Combined sources3
Beta strandi204 – 207Combined sources4
Helixi212 – 221Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G62X-ray2.50A1-224[»]
2X7Nelectron microscopy11.80B1-224[»]
3J2Ielectron microscopy11.90B1-245[»]
3JCTelectron microscopy3.08y1-245[»]
4V7Felectron microscopy8.70m1-245[»]
5FL8electron microscopy9.50m1-245[»]
ProteinModelPortaliQ12522.
SMRiQ12522.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12522.

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-6 family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000015972.
HOGENOMiHOG000230605.
InParanoidiQ12522.
KOiK03264.
OMAiNDWCAFT.
OrthoDBiEOG092C4BDP.

Family and domain databases

CDDicd00527. IF6. 1 hit.
HAMAPiMF_00032. eIF_6. 1 hit.
InterProiIPR002769. eIF6.
[Graphical view]
PANTHERiPTHR10784. PTHR10784. 1 hit.
PfamiPF01912. eIF-6. 1 hit.
[Graphical view]
PIRSFiPIRSF006413. IF-6. 1 hit.
SMARTiSM00654. eIF6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00323. eIF-6. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH
60 70 80 90 100
TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLPD SVKIQRVEER
110 120 130 140 150
LSALGNVICC NDYVALVHPD IDRETEELIS DVLGVEVFRQ TISGNILVGS
160 170 180 190 200
YCSLSNQGGL VHPQTSVQDQ EELSSLLQVP LVAGTVNRGS SVVGAGMVVN
210 220 230 240
DYLAVTGLDT TAPELSVIES IFRLQDAQPE SISGNLRDTL IETYS
Length:245
Mass (Da):26,458
Last modified:November 1, 1996 - v1
Checksum:i1BCE041C61DFD2A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95012.1.
Z49919 Genomic DNA. Translation: CAA90161.1.
U31900 Genomic DNA. Translation: AAA97594.1.
AY692916 Genomic DNA. Translation: AAT92935.1.
BK006949 Genomic DNA. Translation: DAA11442.1.
PIRiS57550.
RefSeqiNP_015341.1. NM_001184113.1.

Genome annotation databases

EnsemblFungiiYPR016C; YPR016C; YPR016C.
GeneIDi856126.
KEGGisce:YPR016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95012.1.
Z49919 Genomic DNA. Translation: CAA90161.1.
U31900 Genomic DNA. Translation: AAA97594.1.
AY692916 Genomic DNA. Translation: AAT92935.1.
BK006949 Genomic DNA. Translation: DAA11442.1.
PIRiS57550.
RefSeqiNP_015341.1. NM_001184113.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G62X-ray2.50A1-224[»]
2X7Nelectron microscopy11.80B1-224[»]
3J2Ielectron microscopy11.90B1-245[»]
3JCTelectron microscopy3.08y1-245[»]
4V7Felectron microscopy8.70m1-245[»]
5FL8electron microscopy9.50m1-245[»]
ProteinModelPortaliQ12522.
SMRiQ12522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36193. 133 interactors.
DIPiDIP-5395N.
IntActiQ12522. 77 interactors.
MINTiMINT-527536.

PTM databases

iPTMnetiQ12522.

Proteomic databases

MaxQBiQ12522.
PRIDEiQ12522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR016C; YPR016C; YPR016C.
GeneIDi856126.
KEGGisce:YPR016C.

Organism-specific databases

EuPathDBiFungiDB:YPR016C.
SGDiS000006220. TIF6.

Phylogenomic databases

GeneTreeiENSGT00390000015972.
HOGENOMiHOG000230605.
InParanoidiQ12522.
KOiK03264.
OMAiNDWCAFT.
OrthoDBiEOG092C4BDP.

Enzyme and pathway databases

BioCyciYEAST:G3O-34176-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12522.
PROiQ12522.

Family and domain databases

CDDicd00527. IF6. 1 hit.
HAMAPiMF_00032. eIF_6. 1 hit.
InterProiIPR002769. eIF6.
[Graphical view]
PANTHERiPTHR10784. PTHR10784. 1 hit.
PfamiPF01912. eIF-6. 1 hit.
[Graphical view]
PIRSFiPIRSF006413. IF-6. 1 hit.
SMARTiSM00654. eIF6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00323. eIF-6. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF6_YEAST
AccessioniPrimary (citable) accession number: Q12522
Secondary accession number(s): D6W426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 18600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.