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Q12514 (NOT5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
General negative regulator of transcription subunit 5
Gene names
Name:NOT5
Ordered Locus Names:YPR072W
ORF Names:YP9499.27
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.6

Subunit structure

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.6 Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasm Probable. Nucleus.

Miscellaneous

Present with 5110 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CNOT2/3/5 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CAF40P538293EBI-12184,EBI-28306

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560General negative regulator of transcription subunit 5
PRO_0000198338

Regions

Coiled coil3 – 2624 Potential
Coiled coil37 – 7135 Potential
Coiled coil124 – 17754 Potential

Amino acid modifications

Modified residue3061Phosphothreonine Ref.11 Ref.13
Modified residue3771Phosphoserine Ref.11 Ref.12 Ref.13

Secondary structure

................................. 560
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12514 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 073137119B3E92BC

FASTA56065,855
        10         20         30         40         50         60 
MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK REIKKLQKHR 

        70         80         90        100        110        120 
DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM KTKQFSKEAL TNPDIIKDPK 

       130        140        150        160        170        180 
ELKKRDQVLF IHDCLDELQK QLEQYEAQEN EEQTERHEFH IANLENILKK LQNNEMDPEP 

       190        200        210        220        230        240 
VEEFQDDIKY YVENNDDPDF IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK 

       250        260        270        280        290        300 
KQERSPKKKA PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 

       310        320        330        340        350        360 
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF QNPLFNDELK 

       370        380        390        400        410        420 
YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP CLYTKPLSLP HPTSIFFPNE 

       430        440        450        460        470        480 
PIRFVYPYDV PLNLTNNEND TDNKFGKDSK AKSKKDDDIY SRTSLARIFM KFDLDTLFFI 

       490        500        510        520        530        540 
FYHYQGSYEQ FLAARELFKN RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS 

       550        560 
WLARRCGNDF VYNEEDFEKL 

« Hide

References

« Hide 'large scale' references
[1]Couch J.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Characterization of NOT5 that encodes a new component of the Not protein complex."
Oberholzer U., Collart M.A.
Gene 207:61-69(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
[7]"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5."
Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.
Mol. Cell. Biol. 19:6642-6651(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NOT1.
[8]"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[9]"Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex."
Russell P., Benson J.D., Denis C.L.
J. Mol. Biol. 322:27-39(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NOT2.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U51033 Genomic DNA. Translation: AAB68123.1.
Z71255 Genomic DNA. Translation: CAA94980.1.
Z49219 Genomic DNA. Translation: CAA89189.1.
AY692887 Genomic DNA. Translation: AAT92906.1.
BK006949 Genomic DNA. Translation: DAA11492.1.
PIRS54093.
RefSeqNP_015397.1. NM_001184169.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80C/F299-560[»]
ProteinModelPortalQ12514.
SMRQ12514. Positions 346-560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36245. 63 interactions.
DIPDIP-2257N.
IntActQ12514. 24 interactions.
MINTMINT-420084.
STRING4932.YPR072W.

Proteomic databases

MaxQBQ12514.
PaxDbQ12514.
PeptideAtlasQ12514.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR072W; YPR072W; YPR072W.
GeneID856186.
KEGGsce:YPR072W.

Organism-specific databases

CYGDYPR072w.
SGDS000006276. NOT5.

Phylogenomic databases

eggNOGCOG5665.
HOGENOMHOG000184850.
KOK12580.
OMAHEFHIAN.
OrthoDBEOG7WQ84Q.

Enzyme and pathway databases

BioCycYEAST:G3O-34219-MONOMER.

Gene expression databases

GenevestigatorQ12514.

Family and domain databases

InterProIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFPIRSF005290. NOT_su_3_5. 1 hit.
ProtoNetSearch...

Other

NextBio981366.
PROQ12514.

Entry information

Entry nameNOT5_YEAST
AccessionPrimary (citable) accession number: Q12514
Secondary accession number(s): D6W476
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references