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Q12514

- NOT5_YEAST

UniProt

Q12514 - NOT5_YEAST

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Protein

General negative regulator of transcription subunit 5

Gene

NOT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

GO - Biological processi

  1. deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  2. nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  3. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  4. protein ubiquitination Source: SGD
  5. regulation of transcription from RNA polymerase II promoter Source: SGD
  6. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 5
Gene namesi
Name:NOT5
Ordered Locus Names:YPR072W
ORF Names:YP9499.27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR072w.
SGDiS000006276. NOT5.

Subcellular locationi

GO - Cellular componenti

  1. CCR4-NOT core complex Source: SGD
  2. cytoplasm Source: SGD
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560General negative regulator of transcription subunit 5PRO_0000198338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei306 – 3061Phosphothreonine2 Publications
Modified residuei377 – 3771Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12514.
PaxDbiQ12514.
PeptideAtlasiQ12514.

Expressioni

Gene expression databases

GenevestigatoriQ12514.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF40P538293EBI-12184,EBI-28306

Protein-protein interaction databases

BioGridi36245. 63 interactions.
DIPiDIP-2257N.
IntActiQ12514. 24 interactions.
MINTiMINT-420084.
STRINGi4932.YPR072W.

Structurei

Secondary structure

1
560
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni348 – 3514
Helixi353 – 3553
Helixi356 – 3649
Helixi367 – 3704
Helixi378 – 38912
Helixi394 – 3974
Turni459 – 4624
Helixi464 – 4696
Helixi474 – 48310
Helixi488 – 50114
Beta strandi504 – 5063
Turni507 – 5093
Beta strandi512 – 5154
Beta strandi532 – 5354
Turni537 – 5404
Beta strandi542 – 5454
Helixi554 – 5563

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80C/F299-560[»]
ProteinModelPortaliQ12514.
SMRiQ12514. Positions 346-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 2624Sequence AnalysisAdd
BLAST
Coiled coili37 – 7135Sequence AnalysisAdd
BLAST
Coiled coili124 – 17754Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5665.
HOGENOMiHOG000184850.
InParanoidiQ12514.
KOiK12580.
OMAiHEFHIAN.
OrthoDBiEOG7WQ84Q.

Family and domain databases

InterProiIPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12514-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK
60 70 80 90 100
REIKKLQKHR DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM
110 120 130 140 150
KTKQFSKEAL TNPDIIKDPK ELKKRDQVLF IHDCLDELQK QLEQYEAQEN
160 170 180 190 200
EEQTERHEFH IANLENILKK LQNNEMDPEP VEEFQDDIKY YVENNDDPDF
210 220 230 240 250
IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK KQERSPKKKA
260 270 280 290 300
PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD
310 320 330 340 350
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF
360 370 380 390 400
QNPLFNDELK YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP
410 420 430 440 450
CLYTKPLSLP HPTSIFFPNE PIRFVYPYDV PLNLTNNEND TDNKFGKDSK
460 470 480 490 500
AKSKKDDDIY SRTSLARIFM KFDLDTLFFI FYHYQGSYEQ FLAARELFKN
510 520 530 540 550
RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS WLARRCGNDF
560
VYNEEDFEKL
Length:560
Mass (Da):65,855
Last modified:November 1, 1996 - v1
Checksum:i073137119B3E92BC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51033 Genomic DNA. Translation: AAB68123.1.
Z71255 Genomic DNA. Translation: CAA94980.1.
Z49219 Genomic DNA. Translation: CAA89189.1.
AY692887 Genomic DNA. Translation: AAT92906.1.
BK006949 Genomic DNA. Translation: DAA11492.1.
PIRiS54093.
RefSeqiNP_015397.1. NM_001184169.1.

Genome annotation databases

EnsemblFungiiYPR072W; YPR072W; YPR072W.
GeneIDi856186.
KEGGisce:YPR072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U51033 Genomic DNA. Translation: AAB68123.1 .
Z71255 Genomic DNA. Translation: CAA94980.1 .
Z49219 Genomic DNA. Translation: CAA89189.1 .
AY692887 Genomic DNA. Translation: AAT92906.1 .
BK006949 Genomic DNA. Translation: DAA11492.1 .
PIRi S54093.
RefSeqi NP_015397.1. NM_001184169.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4BY6 X-ray 2.80 C/F 299-560 [» ]
ProteinModelPortali Q12514.
SMRi Q12514. Positions 346-560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36245. 63 interactions.
DIPi DIP-2257N.
IntActi Q12514. 24 interactions.
MINTi MINT-420084.
STRINGi 4932.YPR072W.

Proteomic databases

MaxQBi Q12514.
PaxDbi Q12514.
PeptideAtlasi Q12514.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR072W ; YPR072W ; YPR072W .
GeneIDi 856186.
KEGGi sce:YPR072W.

Organism-specific databases

CYGDi YPR072w.
SGDi S000006276. NOT5.

Phylogenomic databases

eggNOGi COG5665.
HOGENOMi HOG000184850.
InParanoidi Q12514.
KOi K12580.
OMAi HEFHIAN.
OrthoDBi EOG7WQ84Q.

Enzyme and pathway databases

BioCyci YEAST:G3O-34219-MONOMER.

Miscellaneous databases

NextBioi 981366.
PROi Q12514.

Gene expression databases

Genevestigatori Q12514.

Family and domain databases

InterProi IPR012270. CCR4-NOT_su3/5.
IPR007282. NOT.
IPR007207. Not_N.
[Graphical view ]
Pfami PF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view ]
PIRSFi PIRSF005290. NOT_su_3_5. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Couch J.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Characterization of NOT5 that encodes a new component of the Not protein complex."
    Oberholzer U., Collart M.A.
    Gene 207:61-69(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
    Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
    EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
  7. "The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5."
    Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.
    Mol. Cell. Biol. 19:6642-6651(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOT1.
  8. "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
    Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
    J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
  9. "Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex."
    Russell P., Benson J.D., Denis C.L.
    J. Mol. Biol. 322:27-39(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOT2.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOT5_YEAST
AccessioniPrimary (citable) accession number: Q12514
Secondary accession number(s): D6W476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5110 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3