General negative regulator of transcription subunit 5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12514 (NOT5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
General negative regulator of transcription subunit 5

Gene names

Name:	NOT5
Ordered Locus Names:	YPR072W
ORF Names:	YP9499.27

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.6
Subunit structure	Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.6 Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm Probable. Nucleus.
Miscellaneous	Present with 5110 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CNOT2/3/5 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	deadenylation-independent decapping of nuclear-transcribed mRNA Inferred from mutant phenotype PubMed 15706350. Source: SGD nuclear-transcribed mRNA poly(A) tail shortening Inferred from direct assay PubMed 11889048. Source: SGD positive regulation of transcription elongation from RNA polymerase II promoter Inferred from direct assay PubMed 21406554. Source: SGD protein ubiquitination Inferred from mutant phenotype PubMed 16926149. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from mutant phenotype PubMed 11404327. Source: SGD
Cellular_component	CCR4-NOT core complex Inferred from physical interaction Ref.7. Source: SGD cytoplasm Inferred from direct assay PubMed 11889048. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAF40	P53829	3	EBI-12184,EBI-28306

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 560

560

General negative regulator of transcription subunit 5

PRO_0000198338

Regions

Coiled coil

3 – 26

Potential

Coiled coil

37 – 71

Potential

Coiled coil

124 – 177

Potential

Amino acid modifications

Modified residue

262

Phosphothreonine Ref.13 Ref.14

Modified residue

263

Phosphothreonine Ref.13 Ref.14

Modified residue

275

Phosphoserine Ref.14

Modified residue

277

Phosphoserine Ref.14

Modified residue

306

Phosphothreonine Ref.11 Ref.14

Modified residue

308

Phosphothreonine Ref.14

Modified residue

377

Phosphoserine Ref.11 Ref.12 Ref.14

Sequences

Sequence

Length

Mass (Da)

Tools

Q12514 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 073137119B3E92BC
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FASTA

560

65,855

        10         20         30         40         50         60 
MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK REIKKLQKHR 

        70         80         90        100        110        120 
DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM KTKQFSKEAL TNPDIIKDPK 

       130        140        150        160        170        180 
ELKKRDQVLF IHDCLDELQK QLEQYEAQEN EEQTERHEFH IANLENILKK LQNNEMDPEP 

       190        200        210        220        230        240 
VEEFQDDIKY YVENNDDPDF IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK 

       250        260        270        280        290        300 
KQERSPKKKA PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 

       310        320        330        340        350        360 
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF QNPLFNDELK 

       370        380        390        400        410        420 
YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP CLYTKPLSLP HPTSIFFPNE 

       430        440        450        460        470        480 
PIRFVYPYDV PLNLTNNEND TDNKFGKDSK AKSKKDDDIY SRTSLARIFM KFDLDTLFFI 

       490        500        510        520        530        540 
FYHYQGSYEQ FLAARELFKN RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS 

       550        560 
WLARRCGNDF VYNEEDFEKL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Couch J. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Characterization of NOT5 that encodes a new component of the Not protein complex." Oberholzer U., Collart M.A. Gene 207:61-69(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively." Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L. EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
[7]	"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5." Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L. Mol. Cell. Biol. 19:6642-6651(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NOT1.
[8]	"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex." Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L. J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[9]	"Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex." Russell P., Benson J.D., Denis C.L. J. Mol. Biol. 322:27-39(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NOT2.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-262 AND THR-263, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-262; THR-263; SER-275; SER-277; THR-306; THR-308 AND SER-377, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51033 Genomic DNA. Translation: AAB68123.1. Z71255 Genomic DNA. Translation: CAA94980.1. Z49219 Genomic DNA. Translation: CAA89189.1. AY692887 Genomic DNA. Translation: AAT92906.1. BK006949 Genomic DNA. Translation: DAA11492.1.
PIR	S54093.
RefSeq	NP_015397.1. NM_001184169.1.
3D structure databases
ProteinModelPortal	Q12514.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2257N.
IntAct	Q12514. 22 interactions.
MINT	MINT-420084.
STRING	4932.YPR072W.
Proteomic databases
PaxDb	Q12514.
PeptideAtlas	Q12514.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR072W; YPR072W; YPR072W.
GeneID	856186.
KEGG	sce:YPR072W.
Organism-specific databases
CYGD	YPR072w.
SGD	S000006276. NOT5.
Phylogenomic databases
eggNOG	COG5665.
GeneTree	ENSGT00390000014743.
HOGENOM	HOG000184850.
OMA	HPTSIFF.
OrthoDB	EOG4NW2V3.
Gene expression databases
Genevestigator	Q12514.
GermOnline	YPR072W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012270. CCR4-NOT_su3/5. IPR007282. NOT. IPR007207. Not_N. [Graphical view]
Pfam	PF04153. NOT2_3_5. 1 hit. PF04065. Not3. 1 hit. [Graphical view]
PIRSF	PIRSF005290. NOT_su_3_5. 1 hit.
ProtoNet	Search...
Other
NextBio	981366.

Entry information

Entry name

NOT5_YEAST

Accession

Primary (citable) accession number: Q12514
Secondary accession number(s): D6W476

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents