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Q12514

- NOT5_YEAST

UniProt

Q12514 - NOT5_YEAST

Protein

General negative regulator of transcription subunit 5

Gene

NOT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
    2. nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
    3. positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
    4. protein ubiquitination Source: SGD
    5. regulation of transcription from RNA polymerase II promoter Source: SGD
    6. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34219-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    General negative regulator of transcription subunit 5
    Gene namesi
    Name:NOT5
    Ordered Locus Names:YPR072W
    ORF Names:YP9499.27
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR072w.
    SGDiS000006276. NOT5.

    Subcellular locationi

    GO - Cellular componenti

    1. CCR4-NOT core complex Source: SGD
    2. cytoplasm Source: SGD
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560General negative regulator of transcription subunit 5PRO_0000198338Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei306 – 3061Phosphothreonine2 Publications
    Modified residuei377 – 3771Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12514.
    PaxDbiQ12514.
    PeptideAtlasiQ12514.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12514.

    Interactioni

    Subunit structurei

    Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CAF40P538293EBI-12184,EBI-28306

    Protein-protein interaction databases

    BioGridi36245. 63 interactions.
    DIPiDIP-2257N.
    IntActiQ12514. 24 interactions.
    MINTiMINT-420084.
    STRINGi4932.YPR072W.

    Structurei

    Secondary structure

    1
    560
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni348 – 3514
    Helixi353 – 3553
    Helixi356 – 3649
    Helixi367 – 3704
    Helixi378 – 38912
    Helixi394 – 3974
    Turni459 – 4624
    Helixi464 – 4696
    Helixi474 – 48310
    Helixi488 – 50114
    Beta strandi504 – 5063
    Turni507 – 5093
    Beta strandi512 – 5154
    Beta strandi532 – 5354
    Turni537 – 5404
    Beta strandi542 – 5454
    Helixi554 – 5563

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BY6X-ray2.80C/F299-560[»]
    ProteinModelPortaliQ12514.
    SMRiQ12514. Positions 346-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili3 – 2624Sequence AnalysisAdd
    BLAST
    Coiled coili37 – 7135Sequence AnalysisAdd
    BLAST
    Coiled coili124 – 17754Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CNOT2/3/5 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5665.
    HOGENOMiHOG000184850.
    KOiK12580.
    OMAiHEFHIAN.
    OrthoDBiEOG7WQ84Q.

    Family and domain databases

    InterProiIPR012270. CCR4-NOT_su3/5.
    IPR007282. NOT.
    IPR007207. Not_N.
    [Graphical view]
    PfamiPF04153. NOT2_3_5. 1 hit.
    PF04065. Not3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005290. NOT_su_3_5. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12514-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK    50
    REIKKLQKHR DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM 100
    KTKQFSKEAL TNPDIIKDPK ELKKRDQVLF IHDCLDELQK QLEQYEAQEN 150
    EEQTERHEFH IANLENILKK LQNNEMDPEP VEEFQDDIKY YVENNDDPDF 200
    IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK KQERSPKKKA 250
    PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 300
    NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF 350
    QNPLFNDELK YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP 400
    CLYTKPLSLP HPTSIFFPNE PIRFVYPYDV PLNLTNNEND TDNKFGKDSK 450
    AKSKKDDDIY SRTSLARIFM KFDLDTLFFI FYHYQGSYEQ FLAARELFKN 500
    RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS WLARRCGNDF 550
    VYNEEDFEKL 560
    Length:560
    Mass (Da):65,855
    Last modified:November 1, 1996 - v1
    Checksum:i073137119B3E92BC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51033 Genomic DNA. Translation: AAB68123.1.
    Z71255 Genomic DNA. Translation: CAA94980.1.
    Z49219 Genomic DNA. Translation: CAA89189.1.
    AY692887 Genomic DNA. Translation: AAT92906.1.
    BK006949 Genomic DNA. Translation: DAA11492.1.
    PIRiS54093.
    RefSeqiNP_015397.1. NM_001184169.1.

    Genome annotation databases

    EnsemblFungiiYPR072W; YPR072W; YPR072W.
    GeneIDi856186.
    KEGGisce:YPR072W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U51033 Genomic DNA. Translation: AAB68123.1 .
    Z71255 Genomic DNA. Translation: CAA94980.1 .
    Z49219 Genomic DNA. Translation: CAA89189.1 .
    AY692887 Genomic DNA. Translation: AAT92906.1 .
    BK006949 Genomic DNA. Translation: DAA11492.1 .
    PIRi S54093.
    RefSeqi NP_015397.1. NM_001184169.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BY6 X-ray 2.80 C/F 299-560 [» ]
    ProteinModelPortali Q12514.
    SMRi Q12514. Positions 346-560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36245. 63 interactions.
    DIPi DIP-2257N.
    IntActi Q12514. 24 interactions.
    MINTi MINT-420084.
    STRINGi 4932.YPR072W.

    Proteomic databases

    MaxQBi Q12514.
    PaxDbi Q12514.
    PeptideAtlasi Q12514.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR072W ; YPR072W ; YPR072W .
    GeneIDi 856186.
    KEGGi sce:YPR072W.

    Organism-specific databases

    CYGDi YPR072w.
    SGDi S000006276. NOT5.

    Phylogenomic databases

    eggNOGi COG5665.
    HOGENOMi HOG000184850.
    KOi K12580.
    OMAi HEFHIAN.
    OrthoDBi EOG7WQ84Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34219-MONOMER.

    Miscellaneous databases

    NextBioi 981366.
    PROi Q12514.

    Gene expression databases

    Genevestigatori Q12514.

    Family and domain databases

    InterProi IPR012270. CCR4-NOT_su3/5.
    IPR007282. NOT.
    IPR007207. Not_N.
    [Graphical view ]
    Pfami PF04153. NOT2_3_5. 1 hit.
    PF04065. Not3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005290. NOT_su_3_5. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Couch J.
      Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Characterization of NOT5 that encodes a new component of the Not protein complex."
      Oberholzer U., Collart M.A.
      Gene 207:61-69(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively."
      Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L.
      EMBO J. 17:1096-1106(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
    7. "The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5."
      Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L.
      Mol. Cell. Biol. 19:6642-6651(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOT1.
    8. "Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex."
      Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L.
      J. Mol. Biol. 314:683-694(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
    9. "Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex."
      Russell P., Benson J.D., Denis C.L.
      J. Mol. Biol. 322:27-39(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOT2.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNOT5_YEAST
    AccessioniPrimary (citable) accession number: Q12514
    Secondary accession number(s): D6W476
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5110 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3