Q12514 (NOT5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General negative regulator of transcription subunit 5

Gene names

Name:	NOT5
Ordered Locus Names:	YPR072W
ORF Names:	YP9499.27

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery. Ref.6
Subunit structure	Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex. Ref.7 Ref.9
Subcellular location	Cytoplasm Probable. Nucleus.
Miscellaneous	Present with 5110 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the CNOT2/3/5 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
Molecular function	Activator Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear-transcribed mRNA poly(A) tail shortening Inferred from direct assay. Source: SGD protein ubiquitination Inferred from mutant phenotype. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from physical interaction Ref.7. Source: SGD transcription elongation from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD
Cellular component	CCR4-NOT core complex Inferred from physical interaction Ref.7. Source: SGD cytoplasm Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	General negative regulator of transcription subunit 5		PRO_0000198338
Regions
	Coiled coil	3 – 26	24	Potential
	Coiled coil	37 – 71	35	Potential
	Coiled coil	124 – 177	54	Potential
Amino acid modifications
	Modified residue	262	1	Phosphothreonine Ref.13 Ref.14
	Modified residue	263	1	Phosphothreonine Ref.13 Ref.14
	Modified residue	275	1	Phosphoserine Ref.14
	Modified residue	277	1	Phosphoserine Ref.14
	Modified residue	306	1	Phosphothreonine Ref.11 Ref.14
	Modified residue	308	1	Phosphothreonine Ref.14
	Modified residue	377	1	Phosphoserine Ref.11 Ref.12 Ref.14

Sequences

	Sequence		Length	Mass (Da)
	Q12514 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 073137119B3E92BC	FASTA	560	65,855
	10 20 30 40 50 60 MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK REIKKLQKHR 70 80 90 100 110 120 DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM KTKQFSKEAL TNPDIIKDPK 130 140 150 160 170 180 ELKKRDQVLF IHDCLDELQK QLEQYEAQEN EEQTERHEFH IANLENILKK LQNNEMDPEP 190 200 210 220 230 240 VEEFQDDIKY YVENNDDPDF IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK 250 260 270 280 290 300 KQERSPKKKA PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD 310 320 330 340 350 360 NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF QNPLFNDELK 370 380 390 400 410 420 YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP CLYTKPLSLP HPTSIFFPNE 430 440 450 460 470 480 PIRFVYPYDV PLNLTNNEND TDNKFGKDSK AKSKKDDDIY SRTSLARIFM KFDLDTLFFI 490 500 510 520 530 540 FYHYQGSYEQ FLAARELFKN RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS 550 560 WLARRCGNDF VYNEEDFEKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Couch J. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Characterization of NOT5 that encodes a new component of the Not protein complex." Oberholzer U., Collart M.A. Gene 207:61-69(1998) [PubMed: 9511744] [Abstract] Cited for: CHARACTERIZATION.
[6]	"The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively." Liu H.Y., Badarinarayana V., Audino D.C., Rappsilber J., Mann M., Denis C.L. EMBO J. 17:1096-1106(1998) [PubMed: 9463387] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX, FUNCTION OF THE CCR4-NOT CORE COMPLEX IN TRANSCRIPTIONAL REGULATION.
[7]	"The CCR4 and CAF1 proteins of the CCR4-NOT complex are physically and functionally separated from NOT2, NOT4, and NOT5." Bai Y., Salvadore C., Chiang Y.C., Collart M.A., Liu H.Y., Denis C.L. Mol. Cell. Biol. 19:6642-6651(1999) [PubMed: 10490603] [Abstract] Cited for: INTERACTION WITH NOT1.
[8]	"Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex." Chen J., Rappsilber J., Chiang Y.C., Russell P., Mann M., Denis C.L. J. Mol. Biol. 314:683-694(2001) [PubMed: 11733989] [Abstract] Cited for: IDENTIFICATION IN THE CCR4-NOT CORE COMPLEX.
[9]	"Characterization of mutations in NOT2 indicates that it plays an important role in maintaining the integrity of the CCR4-NOT complex." Russell P., Benson J.D., Denis C.L. J. Mol. Biol. 322:27-39(2002) [PubMed: 12215412] [Abstract] Cited for: INTERACTION WITH NOT2.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-306 AND SER-377, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-262 AND THR-263, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-262; THR-263; SER-275; SER-277; THR-306; THR-308 AND SER-377, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51033 Genomic DNA. Translation: AAB68123.1. Z71255 Genomic DNA. Translation: CAA94980.1. Z49219 Genomic DNA. Translation: CAA89189.1. AY692887 Genomic DNA. Translation: AAT92906.1. BK006949 Genomic DNA. Translation: DAA11492.1.
PIR	S54093.
RefSeq	NP_015397.1. NM_001184169.1.
3D structure databases
ProteinModelPortal	Q12514.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2257N.
IntAct	Q12514. 22 interactions.
MINT	MINT-420084.
STRING	Q12514.
Proteomic databases
PeptideAtlas	Q12514.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR072W; YPR072W; YPR072W.
GeneID	856186.
KEGG	sce:YPR072W.
NMPDR	fig|4932.3.peg.6533.
Organism-specific databases
CYGD	YPR072w.
SGD	S000006276. NOT5.
Phylogenomic databases
eggNOG	fuNOG07695.
GeneTree	EFGT00050000002522.
HOGENOM	HBG395803.
OMA	HEFHIAN.
OrthoDB	EOG4NW2V3.
Gene expression databases
ArrayExpress	Q12514.
Genevestigator	Q12514.
GermOnline	YPR072W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012270. CCR4-NOT_su3/5. IPR007282. NOT. IPR007207. Not_N. [Graphical view]
Pfam	PF04153. NOT2_3_5. 1 hit. PF04065. Not3. 1 hit. [Graphical view]
PIRSF	PIRSF005290. NOT_su_3_5. 1 hit.
ProtoNet	Search...
Other
NextBio	981366.

Entry information

Entry name

NOT5_YEAST

Accession

Primary (citable) accession number: Q12514
Secondary accession number(s): D6W476

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program