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Protein

General negative regulator of transcription subunit 5

Gene

NOT5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.1 Publication

GO - Biological processi

  • deadenylation-dependent decapping of nuclear-transcribed mRNA Source: SGD
  • deadenylation-independent decapping of nuclear-transcribed mRNA Source: SGD
  • negative regulation of translation Source: GO_Central
  • nuclear-transcribed mRNA poly(A) tail shortening Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein ubiquitination Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
General negative regulator of transcription subunit 5
Gene namesi
Name:NOT5
Ordered Locus Names:YPR072W
ORF Names:YP9499.27
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR072W.
SGDiS000006276. NOT5.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT core complex Source: SGD
  • cytoplasm Source: SGD
  • cytoplasmic mRNA processing body Source: GO_Central
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560General negative regulator of transcription subunit 5PRO_0000198338Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei306 – 3061PhosphothreonineCombined sources
Cross-linki338 – 338Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei377 – 3771PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ12514.

PTM databases

iPTMnetiQ12514.

Interactioni

Subunit structurei

Forms a NOT protein complex that comprises NOT1, NOT2, NOT3, NOT4 and NOT5. Subunit of the 1.0 MDa CCR4-NOT core complex that contains CCR4, CAF1, NOT1, NOT2, NOT3, NOT4, NOT5, CAF40 and CAF130. In the complex interacts with NOT1 and NOT2. The core complex probably is part of a less characterized 1.9 MDa CCR4-NOT complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAF40P538293EBI-12184,EBI-28306

Protein-protein interaction databases

BioGridi36245. 62 interactions.
DIPiDIP-2257N.
IntActiQ12514. 24 interactions.
MINTiMINT-420084.

Structurei

Secondary structure

1
560
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni348 – 3514Combined sources
Helixi353 – 3553Combined sources
Helixi356 – 3649Combined sources
Helixi367 – 3704Combined sources
Helixi378 – 38912Combined sources
Helixi394 – 3974Combined sources
Turni459 – 4624Combined sources
Helixi464 – 4696Combined sources
Helixi474 – 48310Combined sources
Helixi488 – 50114Combined sources
Beta strandi504 – 5063Combined sources
Turni507 – 5093Combined sources
Beta strandi512 – 5154Combined sources
Beta strandi532 – 5354Combined sources
Turni537 – 5404Combined sources
Beta strandi542 – 5454Combined sources
Helixi554 – 5563Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80C/F299-560[»]
ProteinModelPortaliQ12514.
SMRiQ12514. Positions 346-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 2624Sequence analysisAdd
BLAST
Coiled coili37 – 7135Sequence analysisAdd
BLAST
Coiled coili124 – 17754Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the CNOT2/3/5 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000014743.
HOGENOMiHOG000184850.
InParanoidiQ12514.
KOiK12580.
OMAiIENGMER.
OrthoDBiEOG092C3ROC.

Family and domain databases

InterProiIPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRKLQQDI DKLLKKVKEG IEDFDDIYEK FQSTDPSNSS HREKLESDLK
60 70 80 90 100
REIKKLQKHR DQIKTWLSKE DVKDKQSVLM TNRRLIENGM ERFKSVEKLM
110 120 130 140 150
KTKQFSKEAL TNPDIIKDPK ELKKRDQVLF IHDCLDELQK QLEQYEAQEN
160 170 180 190 200
EEQTERHEFH IANLENILKK LQNNEMDPEP VEEFQDDIKY YVENNDDPDF
210 220 230 240 250
IEYDTIYEDM GCEIQPSSSN NEAPKEGNNQ TSLSSIRSSK KQERSPKKKA
260 270 280 290 300
PQRDVSISDR ATTPIAPGVE SASQSISSTP TPVSTDTPLH TVKDDSIKFD
310 320 330 340 350
NSTLGTPTTH VSMKKKESEN DSEQQLNFPP DRTDEIRKTI QHDVETNAAF
360 370 380 390 400
QNPLFNDELK YWLDSKRYLM QPLQEMSPKM VSQLESSLLN CPDSLDADSP
410 420 430 440 450
CLYTKPLSLP HPTSIFFPNE PIRFVYPYDV PLNLTNNEND TDNKFGKDSK
460 470 480 490 500
AKSKKDDDIY SRTSLARIFM KFDLDTLFFI FYHYQGSYEQ FLAARELFKN
510 520 530 540 550
RNWLFNKVDR CWYYKEIEKL PPGMGKSEEE SWRYFDYKKS WLARRCGNDF
560
VYNEEDFEKL
Length:560
Mass (Da):65,855
Last modified:November 1, 1996 - v1
Checksum:i073137119B3E92BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA. Translation: AAB68123.1.
Z71255 Genomic DNA. Translation: CAA94980.1.
Z49219 Genomic DNA. Translation: CAA89189.1.
AY692887 Genomic DNA. Translation: AAT92906.1.
BK006949 Genomic DNA. Translation: DAA11492.1.
PIRiS54093.
RefSeqiNP_015397.1. NM_001184169.1.

Genome annotation databases

EnsemblFungiiYPR072W; YPR072W; YPR072W.
GeneIDi856186.
KEGGisce:YPR072W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51033 Genomic DNA. Translation: AAB68123.1.
Z71255 Genomic DNA. Translation: CAA94980.1.
Z49219 Genomic DNA. Translation: CAA89189.1.
AY692887 Genomic DNA. Translation: AAT92906.1.
BK006949 Genomic DNA. Translation: DAA11492.1.
PIRiS54093.
RefSeqiNP_015397.1. NM_001184169.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BY6X-ray2.80C/F299-560[»]
ProteinModelPortaliQ12514.
SMRiQ12514. Positions 346-560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36245. 62 interactions.
DIPiDIP-2257N.
IntActiQ12514. 24 interactions.
MINTiMINT-420084.

PTM databases

iPTMnetiQ12514.

Proteomic databases

MaxQBiQ12514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR072W; YPR072W; YPR072W.
GeneIDi856186.
KEGGisce:YPR072W.

Organism-specific databases

EuPathDBiFungiDB:YPR072W.
SGDiS000006276. NOT5.

Phylogenomic databases

GeneTreeiENSGT00390000014743.
HOGENOMiHOG000184850.
InParanoidiQ12514.
KOiK12580.
OMAiIENGMER.
OrthoDBiEOG092C3ROC.

Enzyme and pathway databases

BioCyciYEAST:G3O-34219-MONOMER.

Miscellaneous databases

PROiQ12514.

Family and domain databases

InterProiIPR007282. NOT.
IPR007207. Not_N.
[Graphical view]
PfamiPF04153. NOT2_3_5. 1 hit.
PF04065. Not3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOT5_YEAST
AccessioniPrimary (citable) accession number: Q12514
Secondary accession number(s): D6W476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5110 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.