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Protein

[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial

Gene

PTC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex.2 Publications

Catalytic activityi

[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi197 – 1971Manganese 1By similarity
Metal bindingi197 – 1971Manganese 2By similarity
Metal bindingi198 – 1981Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi424 – 4241Manganese 2By similarity
Metal bindingi480 – 4801Manganese 2By similarity

GO - Molecular functioni

  1. [pyruvate dehydrogenase (lipoamide)] phosphatase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. positive regulation of catalytic activity Source: SGD
  2. protein dephosphorylation Source: SGD
  3. regulation of catalytic activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (EC:3.1.3.43)
Short name:
PDP 1
Alternative name(s):
Phosphatase two C protein 5
Protein phosphatase 2C homolog 5
Short name:
PP2C-5
Protein phosphatase of PDH protein 1
Pyruvate dehydrogenase complex phosphatase 1
Short name:
PDC phosphatase 1
Gene namesi
Name:PTC5
Synonyms:PPP1
Ordered Locus Names:YOR090C
ORF Names:YOR3157C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR090c.
EuPathDBiFungiDB:YOR090C.
SGDiS000005616. PTC5.

Subcellular locationi

  1. Mitochondrion intermembrane space 3 Publications

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 572[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrialPRO_0000057778
Transit peptidei1 – ?MitochondrionSequence Analysis

Post-translational modificationi

Processed by mitochondrial inner membrane protease (IMP) complex and released to the intermembrane space.

Proteomic databases

MaxQBiQ12511.
PaxDbiQ12511.
PeptideAtlasiQ12511.

Expressioni

Gene expression databases

GenevestigatoriQ12511.

Interactioni

Protein-protein interaction databases

BioGridi34488. 44 interactions.
DIPiDIP-6439N.
IntActiQ12511. 6 interactions.
MINTiMINT-672855.
STRINGi4932.YOR090C.

Structurei

3D structure databases

ProteinModelPortaliQ12511.
SMRiQ12511. Positions 149-543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini153 – 543391PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00390000006874.
HOGENOMiHOG000209682.
InParanoidiQ12511.
KOiK01102.
OMAiVHLHVAN.
OrthoDBiEOG7XWPXK.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12511-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPLTRTVAI KKTVKVLSKC QSGREYTQKF LQRAYSTSHA NSTYYSRTKL
60 70 80 90 100
FISSHSKALN IALLSGSLLL TYSYYSPKKI LSLDTINGIK DYSTNTSGNI
110 120 130 140 150
NMPSPNPKGT ETQKSQRSQN DQSVLILNDS KIEAKLHDRE ESHFVNRGTG
160 170 180 190 200
IFRYDVAQLP SNHPIEDDHV EQIITIPIES EDGKSIEKDL YFFGIFDGHG
210 220 230 240 250
GPFTSEKLSK DLVRYVAYQL GQVYDQNKTV FHSDPNQLID SAISKGFLKL
260 270 280 290 300
DNDLVIESFR KLFQDPNNTN IANTLPAISG SCALLSLYNS TNSILKVAVT
310 320 330 340 350
GDSRALICGL DNEGNWTVKS LSTDQTGDNL DEVRRIRKEH PGEPNVIRNG
360 370 380 390 400
RILGSLQPSR AFGDYRYKIK EVDGKPLSDL PEVAKLYFRR EPRDFKTPPY
410 420 430 440 450
VTAEPVITSA KIGENTKFMV MGSDGLFELL TNEEIASLVI RWMDKNMNLA
460 470 480 490 500
PVKAEPGKLP KVIDVSEDKE AQRPAFRYKD NNSSSPSGSN PEYLIEDKNV
510 520 530 540 550
ATHLIRNALS AGGRKEYVSA LVSIPSPMSR RYRDDLTVTV AFFGDSGTPS
560 570
IVSNATSIVM NPEATTKPKP RL
Length:572
Mass (Da):63,669
Last modified:November 1, 1996 - v1
Checksum:i07A5AF33FA72CC2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64011.1.
Z74998 Genomic DNA. Translation: CAA99287.1.
BK006948 Genomic DNA. Translation: DAA10867.1.
PIRiS61650.
RefSeqiNP_014733.1. NM_001183509.1.

Genome annotation databases

EnsemblFungiiYOR090C; YOR090C; YOR090C.
GeneIDi854257.
KEGGisce:YOR090C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64011.1.
Z74998 Genomic DNA. Translation: CAA99287.1.
BK006948 Genomic DNA. Translation: DAA10867.1.
PIRiS61650.
RefSeqiNP_014733.1. NM_001183509.1.

3D structure databases

ProteinModelPortaliQ12511.
SMRiQ12511. Positions 149-543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34488. 44 interactions.
DIPiDIP-6439N.
IntActiQ12511. 6 interactions.
MINTiMINT-672855.
STRINGi4932.YOR090C.

Proteomic databases

MaxQBiQ12511.
PaxDbiQ12511.
PeptideAtlasiQ12511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR090C; YOR090C; YOR090C.
GeneIDi854257.
KEGGisce:YOR090C.

Organism-specific databases

CYGDiYOR090c.
EuPathDBiFungiDB:YOR090C.
SGDiS000005616. PTC5.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00390000006874.
HOGENOMiHOG000209682.
InParanoidiQ12511.
KOiK01102.
OMAiVHLHVAN.
OrthoDBiEOG7XWPXK.

Enzyme and pathway databases

BioCyciYEAST:G3O-33624-MONOMER.

Miscellaneous databases

NextBioi976185.
PROiQ12511.

Gene expression databases

GenevestigatoriQ12511.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  6. "YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex."
    Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G.
    FEBS Lett. 580:2553-2560(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases."
    Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U.
    J. Biol. Chem. 283:9759-9767(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION, POST-TRANSLATIONAL MODIFICATION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPDP1_YEAST
AccessioniPrimary (citable) accession number: Q12511
Secondary accession number(s): D6W2F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.