Q12511 (PP2C5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C homolog 5
Short name=PP2C-5
EC=3.1.3.16

Alternative name(s):
Phosphatase two C protein 5
Protein phosphatase of PDH protein 5

Gene names

Name:	PTC5
Synonyms:	PPP1
Ordered Locus Names:	YOR090C
ORF Names:	YOR3157C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in regulation of pyruvate dehydrogenase activity. Ref.6
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein Potential Ref.5 Ref.6.
Miscellaneous	Present with 7550 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	positive regulation of catalytic activity Inferred from mutant phenotype. Source: SGD protein dephosphorylation Inferred from direct assay. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell protein serine/threonine phosphatase complex Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Protein phosphatase 2C homolog 5		PRO_0000057778
Regions
	Transmembrane	58 – 75	18	Helical; Potential
	Domain	143 – 572	430	PP2C-like
Sites
	Metal binding	197	1	Manganese 1 By similarity
	Metal binding	197	1	Manganese 2 By similarity
	Metal binding	198	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	424	1	Manganese 2 By similarity
	Metal binding	480	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12511 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 07A5AF33FA72CC2A	FASTA	572	63,669
	10 20 30 40 50 60 MSPLTRTVAI KKTVKVLSKC QSGREYTQKF LQRAYSTSHA NSTYYSRTKL FISSHSKALN 70 80 90 100 110 120 IALLSGSLLL TYSYYSPKKI LSLDTINGIK DYSTNTSGNI NMPSPNPKGT ETQKSQRSQN 130 140 150 160 170 180 DQSVLILNDS KIEAKLHDRE ESHFVNRGTG IFRYDVAQLP SNHPIEDDHV EQIITIPIES 190 200 210 220 230 240 EDGKSIEKDL YFFGIFDGHG GPFTSEKLSK DLVRYVAYQL GQVYDQNKTV FHSDPNQLID 250 260 270 280 290 300 SAISKGFLKL DNDLVIESFR KLFQDPNNTN IANTLPAISG SCALLSLYNS TNSILKVAVT 310 320 330 340 350 360 GDSRALICGL DNEGNWTVKS LSTDQTGDNL DEVRRIRKEH PGEPNVIRNG RILGSLQPSR 370 380 390 400 410 420 AFGDYRYKIK EVDGKPLSDL PEVAKLYFRR EPRDFKTPPY VTAEPVITSA KIGENTKFMV 430 440 450 460 470 480 MGSDGLFELL TNEEIASLVI RWMDKNMNLA PVKAEPGKLP KVIDVSEDKE AQRPAFRYKD 490 500 510 520 530 540 NNSSSPSGSN PEYLIEDKNV ATHLIRNALS AGGRKEYVSA LVSIPSPMSR RYRDDLTVTV 550 560 570 AFFGDSGTPS IVSNATSIVM NPEATTKPKP RL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[6]	"YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex." Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G. FEBS Lett. 580:2553-2560(2006) [PubMed: 16643908] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94335 Genomic DNA. Translation: CAA64011.1. Z74998 Genomic DNA. Translation: CAA99287.1. BK006948 Genomic DNA. Translation: DAA10867.1.
PIR	S61650.
RefSeq	NP_014733.1. NM_001183509.1.
3D structure databases
ProteinModelPortal	Q12511.
SMR	Q12511. Positions 127-547.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6439N.
IntAct	Q12511. 8 interactions.
MINT	MINT-672855.
STRING	Q12511.
Proteomic databases
PeptideAtlas	Q12511.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR090C; YOR090C; YOR090C.
GeneID	854257.
KEGG	sce:YOR090C.
NMPDR	fig|4932.3.peg.5835.
Organism-specific databases
CYGD	YOR090c.
SGD	S000005616. PTC5.
Phylogenomic databases
eggNOG	fuNOG04866.
GeneTree	EFGT00050000002543.
HOGENOM	HBG676973.
OMA	YVTAEPV.
OrthoDB	EOG4MWCFT.
Gene expression databases
ArrayExpress	Q12511.
Genevestigator	Q12511.
GermOnline	YOR090C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
Gene3D	G3DSA:3.60.40.10. PP2C-related. 2 hits.
PANTHER	PTHR13832. PP2C. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976185.

Entry information

Entry name

PP2C5_YEAST

Accession

Primary (citable) accession number: Q12511
Secondary accession number(s): D6W2F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program