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Q12511

- PDP1_YEAST

UniProt

Q12511 - PDP1_YEAST

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Protein
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
Gene
PTC5, PPP1, YOR090C, YOR3157C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex.2 Publications

Catalytic activityi

[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi197 – 1971Manganese 1 By similarity
Metal bindingi197 – 1971Manganese 2 By similarity
Metal bindingi198 – 1981Manganese 1; via carbonyl oxygen By similarity
Metal bindingi424 – 4241Manganese 2 By similarity
Metal bindingi480 – 4801Manganese 2 By similarity

GO - Molecular functioni

  1. [pyruvate dehydrogenase (lipoamide)] phosphatase activity Source: SGD
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. positive regulation of catalytic activity Source: SGD
  2. protein dephosphorylation Source: SGD
  3. regulation of catalytic activity Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33624-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (EC:3.1.3.43)
Short name:
PDP 1
Alternative name(s):
Phosphatase two C protein 5
Protein phosphatase 2C homolog 5
Short name:
PP2C-5
Protein phosphatase of PDH protein 1
Pyruvate dehydrogenase complex phosphatase 1
Short name:
PDC phosphatase 1
Gene namesi
Name:PTC5
Synonyms:PPP1
Ordered Locus Names:YOR090C
ORF Names:YOR3157C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR090c.
SGDiS000005616. PTC5.

Subcellular locationi

Mitochondrion intermembrane space 3 Publications

GO - Cellular componenti

  1. mitochondrial intermembrane space Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 572[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrialPRO_0000057778
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Post-translational modificationi

Processed by mitochondrial inner membrane protease (IMP) complex and released to the intermembrane space.

Proteomic databases

MaxQBiQ12511.
PaxDbiQ12511.
PeptideAtlasiQ12511.

Expressioni

Gene expression databases

GenevestigatoriQ12511.

Interactioni

Protein-protein interaction databases

BioGridi34488. 40 interactions.
DIPiDIP-6439N.
IntActiQ12511. 6 interactions.
MINTiMINT-672855.
STRINGi4932.YOR090C.

Structurei

3D structure databases

ProteinModelPortaliQ12511.
SMRiQ12511. Positions 149-543.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 572430PP2C-like
Add
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00390000006874.
HOGENOMiHOG000209682.
KOiK01102.
OMAiVHLHVAN.
OrthoDBiEOG7XWPXK.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12511-1 [UniParc]FASTAAdd to Basket

« Hide

MSPLTRTVAI KKTVKVLSKC QSGREYTQKF LQRAYSTSHA NSTYYSRTKL    50
FISSHSKALN IALLSGSLLL TYSYYSPKKI LSLDTINGIK DYSTNTSGNI 100
NMPSPNPKGT ETQKSQRSQN DQSVLILNDS KIEAKLHDRE ESHFVNRGTG 150
IFRYDVAQLP SNHPIEDDHV EQIITIPIES EDGKSIEKDL YFFGIFDGHG 200
GPFTSEKLSK DLVRYVAYQL GQVYDQNKTV FHSDPNQLID SAISKGFLKL 250
DNDLVIESFR KLFQDPNNTN IANTLPAISG SCALLSLYNS TNSILKVAVT 300
GDSRALICGL DNEGNWTVKS LSTDQTGDNL DEVRRIRKEH PGEPNVIRNG 350
RILGSLQPSR AFGDYRYKIK EVDGKPLSDL PEVAKLYFRR EPRDFKTPPY 400
VTAEPVITSA KIGENTKFMV MGSDGLFELL TNEEIASLVI RWMDKNMNLA 450
PVKAEPGKLP KVIDVSEDKE AQRPAFRYKD NNSSSPSGSN PEYLIEDKNV 500
ATHLIRNALS AGGRKEYVSA LVSIPSPMSR RYRDDLTVTV AFFGDSGTPS 550
IVSNATSIVM NPEATTKPKP RL 572
Length:572
Mass (Da):63,669
Last modified:November 1, 1996 - v1
Checksum:i07A5AF33FA72CC2A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94335 Genomic DNA. Translation: CAA64011.1.
Z74998 Genomic DNA. Translation: CAA99287.1.
BK006948 Genomic DNA. Translation: DAA10867.1.
PIRiS61650.
RefSeqiNP_014733.1. NM_001183509.1.

Genome annotation databases

EnsemblFungiiYOR090C; YOR090C; YOR090C.
GeneIDi854257.
KEGGisce:YOR090C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94335 Genomic DNA. Translation: CAA64011.1 .
Z74998 Genomic DNA. Translation: CAA99287.1 .
BK006948 Genomic DNA. Translation: DAA10867.1 .
PIRi S61650.
RefSeqi NP_014733.1. NM_001183509.1.

3D structure databases

ProteinModelPortali Q12511.
SMRi Q12511. Positions 149-543.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34488. 40 interactions.
DIPi DIP-6439N.
IntActi Q12511. 6 interactions.
MINTi MINT-672855.
STRINGi 4932.YOR090C.

Proteomic databases

MaxQBi Q12511.
PaxDbi Q12511.
PeptideAtlasi Q12511.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR090C ; YOR090C ; YOR090C .
GeneIDi 854257.
KEGGi sce:YOR090C.

Organism-specific databases

CYGDi YOR090c.
SGDi S000005616. PTC5.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00390000006874.
HOGENOMi HOG000209682.
KOi K01102.
OMAi VHLHVAN.
OrthoDBi EOG7XWPXK.

Enzyme and pathway databases

BioCyci YEAST:G3O-33624-MONOMER.

Miscellaneous databases

NextBioi 976185.

Gene expression databases

Genevestigatori Q12511.

Family and domain databases

Gene3Di 3.60.40.10. 2 hits.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 2 hits.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  6. "YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex."
    Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G.
    FEBS Lett. 580:2553-2560(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases."
    Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U.
    J. Biol. Chem. 283:9759-9767(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION, POST-TRANSLATIONAL MODIFICATION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPDP1_YEAST
AccessioniPrimary (citable) accession number: Q12511
Secondary accession number(s): D6W2F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7550 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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