Protein phosphatase 2C homolog 5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12511 (PP2C5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C homolog 5
Short name=PP2C-5
EC=3.1.3.16

Alternative name(s):
Phosphatase two C protein 5
Protein phosphatase of PDH protein 5

Gene names

Name:	PTC5
Synonyms:	PPP1
Ordered Locus Names:	YOR090C
ORF Names:	YOR3157C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in regulation of pyruvate dehydrogenase activity. Ref.6
Catalytic activity	A phosphoprotein + H₂O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subcellular location	Mitochondrion membrane; Single-pass membrane protein Potential Ref.5 Ref.6.
Miscellaneous	Present with 7550 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of catalytic activity Inferred from mutant phenotype PubMed 18180296. Source: SGD protein dephosphorylation Inferred from direct assay PubMed 10580002. Source: SGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial intermembrane space Inferred from direct assay PubMed 22984289. Source: SGD mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	[pyruvate dehydrogenase (lipoamide)] phosphatase activity Inferred from sequence or structural similarity Ref.6. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 10580002. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 572

572

Protein phosphatase 2C homolog 5

PRO_0000057778

Regions

Transmembrane

58 – 75

Helical; Potential

Domain

143 – 572

430

PP2C-like

Sites

Metal binding

197

Manganese 1 By similarity

Metal binding

197

Manganese 2 By similarity

Metal binding

198

Manganese 1; via carbonyl oxygen By similarity

Metal binding

424

Manganese 2 By similarity

Metal binding

480

Manganese 2 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q12511 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 07A5AF33FA72CC2A
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FASTA

572

63,669

        10         20         30         40         50         60 
MSPLTRTVAI KKTVKVLSKC QSGREYTQKF LQRAYSTSHA NSTYYSRTKL FISSHSKALN 

        70         80         90        100        110        120 
IALLSGSLLL TYSYYSPKKI LSLDTINGIK DYSTNTSGNI NMPSPNPKGT ETQKSQRSQN 

       130        140        150        160        170        180 
DQSVLILNDS KIEAKLHDRE ESHFVNRGTG IFRYDVAQLP SNHPIEDDHV EQIITIPIES 

       190        200        210        220        230        240 
EDGKSIEKDL YFFGIFDGHG GPFTSEKLSK DLVRYVAYQL GQVYDQNKTV FHSDPNQLID 

       250        260        270        280        290        300 
SAISKGFLKL DNDLVIESFR KLFQDPNNTN IANTLPAISG SCALLSLYNS TNSILKVAVT 

       310        320        330        340        350        360 
GDSRALICGL DNEGNWTVKS LSTDQTGDNL DEVRRIRKEH PGEPNVIRNG RILGSLQPSR 

       370        380        390        400        410        420 
AFGDYRYKIK EVDGKPLSDL PEVAKLYFRR EPRDFKTPPY VTAEPVITSA KIGENTKFMV 

       430        440        450        460        470        480 
MGSDGLFELL TNEEIASLVI RWMDKNMNLA PVKAEPGKLP KVIDVSEDKE AQRPAFRYKD 

       490        500        510        520        530        540 
NNSSSPSGSN PEYLIEDKNV ATHLIRNALS AGGRKEYVSA LVSIPSPMSR RYRDDLTVTV 

       550        560        570 
AFFGDSGTPS IVSNATSIVM NPEATTKPKP RL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[6]	"YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex." Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G. FEBS Lett. 580:2553-2560(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94335 Genomic DNA. Translation: CAA64011.1. Z74998 Genomic DNA. Translation: CAA99287.1. BK006948 Genomic DNA. Translation: DAA10867.1.
PIR	S61650.
RefSeq	NP_014733.1. NM_001183509.1.
3D structure databases
ProteinModelPortal	Q12511.
SMR	Q12511. Positions 149-543.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6439N.
IntAct	Q12511. 8 interactions.
MINT	MINT-672855.
STRING	4932.YOR090C.
Proteomic databases
PaxDb	Q12511.
PeptideAtlas	Q12511.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR090C; YOR090C; YOR090C.
GeneID	854257.
KEGG	sce:YOR090C.
Organism-specific databases
CYGD	YOR090c.
SGD	S000005616. PTC5.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00390000006874.
HOGENOM	HOG000209682.
OMA	YVTAEPV.
OrthoDB	EOG4MWCFT.
Gene expression databases
Genevestigator	Q12511.
GermOnline	YOR090C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.40.10. 2 hits.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976185.

Entry information

Entry name

PP2C5_YEAST

Accession

Primary (citable) accession number: Q12511
Secondary accession number(s): D6W2F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents