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Q12511

- PDP1_YEAST

UniProt

Q12511 - PDP1_YEAST

Protein

[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial

Gene

PTC5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the dephosphorylation and concomitant reactivation of the E1 alpha subunit (PDA1) of the pyruvate dehydrogenase complex.2 Publications

    Catalytic activityi

    [Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi197 – 1971Manganese 1By similarity
    Metal bindingi197 – 1971Manganese 2By similarity
    Metal bindingi198 – 1981Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi424 – 4241Manganese 2By similarity
    Metal bindingi480 – 4801Manganese 2By similarity

    GO - Molecular functioni

    1. [pyruvate dehydrogenase (lipoamide)] phosphatase activity Source: SGD
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. positive regulation of catalytic activity Source: SGD
    2. protein dephosphorylation Source: SGD
    3. regulation of catalytic activity Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33624-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (EC:3.1.3.43)
    Short name:
    PDP 1
    Alternative name(s):
    Phosphatase two C protein 5
    Protein phosphatase 2C homolog 5
    Short name:
    PP2C-5
    Protein phosphatase of PDH protein 1
    Pyruvate dehydrogenase complex phosphatase 1
    Short name:
    PDC phosphatase 1
    Gene namesi
    Name:PTC5
    Synonyms:PPP1
    Ordered Locus Names:YOR090C
    ORF Names:YOR3157C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR090c.
    SGDiS000005616. PTC5.

    Subcellular locationi

    Mitochondrion intermembrane space 3 Publications

    GO - Cellular componenti

    1. mitochondrial intermembrane space Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 572[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrialPRO_0000057778
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Post-translational modificationi

    Processed by mitochondrial inner membrane protease (IMP) complex and released to the intermembrane space.

    Proteomic databases

    MaxQBiQ12511.
    PaxDbiQ12511.
    PeptideAtlasiQ12511.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12511.

    Interactioni

    Protein-protein interaction databases

    BioGridi34488. 40 interactions.
    DIPiDIP-6439N.
    IntActiQ12511. 6 interactions.
    MINTiMINT-672855.
    STRINGi4932.YOR090C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12511.
    SMRiQ12511. Positions 149-543.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini143 – 572430PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00390000006874.
    HOGENOMiHOG000209682.
    KOiK01102.
    OMAiVHLHVAN.
    OrthoDBiEOG7XWPXK.

    Family and domain databases

    Gene3Di3.60.40.10. 2 hits.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 2 hits.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12511-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPLTRTVAI KKTVKVLSKC QSGREYTQKF LQRAYSTSHA NSTYYSRTKL    50
    FISSHSKALN IALLSGSLLL TYSYYSPKKI LSLDTINGIK DYSTNTSGNI 100
    NMPSPNPKGT ETQKSQRSQN DQSVLILNDS KIEAKLHDRE ESHFVNRGTG 150
    IFRYDVAQLP SNHPIEDDHV EQIITIPIES EDGKSIEKDL YFFGIFDGHG 200
    GPFTSEKLSK DLVRYVAYQL GQVYDQNKTV FHSDPNQLID SAISKGFLKL 250
    DNDLVIESFR KLFQDPNNTN IANTLPAISG SCALLSLYNS TNSILKVAVT 300
    GDSRALICGL DNEGNWTVKS LSTDQTGDNL DEVRRIRKEH PGEPNVIRNG 350
    RILGSLQPSR AFGDYRYKIK EVDGKPLSDL PEVAKLYFRR EPRDFKTPPY 400
    VTAEPVITSA KIGENTKFMV MGSDGLFELL TNEEIASLVI RWMDKNMNLA 450
    PVKAEPGKLP KVIDVSEDKE AQRPAFRYKD NNSSSPSGSN PEYLIEDKNV 500
    ATHLIRNALS AGGRKEYVSA LVSIPSPMSR RYRDDLTVTV AFFGDSGTPS 550
    IVSNATSIVM NPEATTKPKP RL 572
    Length:572
    Mass (Da):63,669
    Last modified:November 1, 1996 - v1
    Checksum:i07A5AF33FA72CC2A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94335 Genomic DNA. Translation: CAA64011.1.
    Z74998 Genomic DNA. Translation: CAA99287.1.
    BK006948 Genomic DNA. Translation: DAA10867.1.
    PIRiS61650.
    RefSeqiNP_014733.1. NM_001183509.1.

    Genome annotation databases

    EnsemblFungiiYOR090C; YOR090C; YOR090C.
    GeneIDi854257.
    KEGGisce:YOR090C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94335 Genomic DNA. Translation: CAA64011.1 .
    Z74998 Genomic DNA. Translation: CAA99287.1 .
    BK006948 Genomic DNA. Translation: DAA10867.1 .
    PIRi S61650.
    RefSeqi NP_014733.1. NM_001183509.1.

    3D structure databases

    ProteinModelPortali Q12511.
    SMRi Q12511. Positions 149-543.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34488. 40 interactions.
    DIPi DIP-6439N.
    IntActi Q12511. 6 interactions.
    MINTi MINT-672855.
    STRINGi 4932.YOR090C.

    Proteomic databases

    MaxQBi Q12511.
    PaxDbi Q12511.
    PeptideAtlasi Q12511.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR090C ; YOR090C ; YOR090C .
    GeneIDi 854257.
    KEGGi sce:YOR090C.

    Organism-specific databases

    CYGDi YOR090c.
    SGDi S000005616. PTC5.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00390000006874.
    HOGENOMi HOG000209682.
    KOi K01102.
    OMAi VHLHVAN.
    OrthoDBi EOG7XWPXK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33624-MONOMER.

    Miscellaneous databases

    NextBioi 976185.

    Gene expression databases

    Genevestigatori Q12511.

    Family and domain databases

    Gene3Di 3.60.40.10. 2 hits.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 2 hits.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    6. "YIL042c and YOR090c encode the kinase and phosphatase of the Saccharomyces cerevisiae pyruvate dehydrogenase complex."
      Krause-Buchholz U., Gey U., Wunschmann J., Becker S., Rodel G.
      FEBS Lett. 580:2553-2560(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases."
      Gey U., Czupalla C., Hoflack B., Rodel G., Krause-Buchholz U.
      J. Biol. Chem. 283:9759-9767(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. Cited for: SUBCELLULAR LOCATION, POST-TRANSLATIONAL MODIFICATION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPDP1_YEAST
    AccessioniPrimary (citable) accession number: Q12511
    Secondary accession number(s): D6W2F1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3