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Protein

DNA damage-binding protein CMR1

Gene

CMR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding protein that binds to both single- and double-stranded DNA. Binds preferentially to UV-damaged DNA in vitro (PubMed:22367945). May be involved in DNA-metabolic processes (PubMed:23349438).1 Publication1 Publication

GO - Molecular functioni

  • DNA binding Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB-KW
  • regulation of DNA damage checkpoint Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29551-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-binding protein CMR11 Publication
Alternative name(s):
Changed mutation rate protein 11 Publication
Gene namesi
Name:CMR11 Publication
Ordered Locus Names:YDL156WImported
ORF Names:D1536
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL156W.
SGDiS000002315. CMR1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear chromatin Source: SGD
  • nuclear periphery Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522DNA damage-binding protein CMR1PRO_0000240874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphoserineCombined sources
Modified residuei69 – 691PhosphothreonineCombined sources
Modified residuei224 – 2241PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12510.
PeptideAtlasiQ12510.

PTM databases

iPTMnetiQ12510.

Expressioni

Inductioni

Closely coexpressed with replication factor A (RF-A), the cohesion complex and the DNA polymerases alpha, delta and epsilon.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LSB3P436032EBI-35343,EBI-22980
YSC84P327932EBI-35343,EBI-24460

Protein-protein interaction databases

BioGridi31907. 679 interactions.
DIPiDIP-1004N.
IntActiQ12510. 17 interactions.
MINTiMINT-560906.

Structurei

3D structure databases

ProteinModelPortaliQ12510.
SMRiQ12510. Positions 188-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati183 – 22442WD 1Sequence analysisAdd
BLAST
Repeati239 – 28143WD 2Sequence analysisAdd
BLAST
Repeati287 – 32741WD 3Sequence analysisAdd
BLAST
Repeati331 – 37141WD 4Sequence analysisAdd
BLAST
Repeati388 – 42740WD 5Sequence analysisAdd
BLAST
Repeati442 – 48140WD 6Sequence analysisAdd
BLAST
Repeati482 – 52140WD 7Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat DDB2/WDR76 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00510000048144.
HOGENOMiHOG000234710.
InParanoidiQ12510.
OMAiTVVRHNC.
OrthoDBiEOG7K6Q40.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR033052. Cmr1/WDR76.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR14773:SF0. PTHR14773:SF0. 1 hit.
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12510-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELTEFQKK RLENIKRNND LLKKLHLSGV ASQIKHEAGV LEKSRAPAKK
60 70 80 90 100
KQKTTNTRAT KSASPTLPTR RSRRLRGESA DDVKGIPNVN DNQLLKMGSP
110 120 130 140 150
DGQDKNFIDA IKEKPVIGDV KLSDLIKDED ESALLEKFKR FNNGNFSSGD
160 170 180 190 200
FFEEIKKRQG DVTGMDEFDL DLYDVFQPNE IKITYERISA TYFHPAMEKK
210 220 230 240 250
LIIAGDTSGT VGFWNVRDEP LADSEEDRME EPDITRVKLF TKNVGRIDCF
260 270 280 290 300
PADTSKILLT SYDGSIRSVH LNNLQSEEVL TLKNEYDDSL GISDCQFSYE
310 320 330 340 350
NPNVLFLTTL GGEFTTFDTR VKKSEYNLRR LADKKIGSMA INPMRPYEIA
360 370 380 390 400
TGSLDRTLKI WDTRNLVKKP EWSQYEDYPS HEIVSTYDSR LSVSAVSYSP
410 420 430 440 450
TDGTLVCNGY DDTIRLFDVK SRDHLSAKLE PKLTIQHNCQ TGRWTSILKA
460 470 480 490 500
RFKPNKNVFA IANMKRAIDI YNSEGQQLAH LPTATVPAVI SWHPLRNWIA
510 520
GGNSSGKIFL FTDDSGTIKQ EE
Length:522
Mass (Da):59,156
Last modified:November 1, 1996 - v1
Checksum:i3BE716E4858EF3E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97751 Genomic DNA. Translation: CAA66335.1.
Z74204 Genomic DNA. Translation: CAA98730.1.
BK006938 Genomic DNA. Translation: DAA11704.1.
PIRiS67704.
RefSeqiNP_010125.1. NM_001180216.1.

Genome annotation databases

EnsemblFungiiYDL156W; YDL156W; YDL156W.
GeneIDi851399.
KEGGisce:YDL156W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97751 Genomic DNA. Translation: CAA66335.1.
Z74204 Genomic DNA. Translation: CAA98730.1.
BK006938 Genomic DNA. Translation: DAA11704.1.
PIRiS67704.
RefSeqiNP_010125.1. NM_001180216.1.

3D structure databases

ProteinModelPortaliQ12510.
SMRiQ12510. Positions 188-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31907. 679 interactions.
DIPiDIP-1004N.
IntActiQ12510. 17 interactions.
MINTiMINT-560906.

PTM databases

iPTMnetiQ12510.

Proteomic databases

MaxQBiQ12510.
PeptideAtlasiQ12510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL156W; YDL156W; YDL156W.
GeneIDi851399.
KEGGisce:YDL156W.

Organism-specific databases

EuPathDBiFungiDB:YDL156W.
SGDiS000002315. CMR1.

Phylogenomic databases

GeneTreeiENSGT00510000048144.
HOGENOMiHOG000234710.
InParanoidiQ12510.
OMAiTVVRHNC.
OrthoDBiEOG7K6Q40.

Enzyme and pathway databases

BioCyciYEAST:G3O-29551-MONOMER.

Miscellaneous databases

NextBioi968564.
PROiQ12510.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR033052. Cmr1/WDR76.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR14773:SF0. PTHR14773:SF0. 1 hit.
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 23 kb region on the left arm of yeast chromosome IV."
    Delaveau T.T.D., Blugeon C., Jacq C., Perea J.
    Yeast 12:1587-1592(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90840 / EAY235 / FY23.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-69, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-64 AND SER-224, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Saccharomyces cerevisiae Cmr1 protein preferentially binds to UV-damaged DNA in vitro."
    Choi D.H., Kwon S.H., Kim J.H., Bae S.H.
    J. Microbiol. 50:112-118(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Yeast gene CMR1/YDL156W is consistently co-expressed with genes participating in DNA-metabolic processes in a variety of stringent clustering experiments."
    Abu-Jamous B., Fa R., Roberts D.J., Nandi A.K.
    J. R. Soc. Interface 10:20120990-20120990(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCMR1_YEAST
AccessioniPrimary (citable) accession number: Q12510
Secondary accession number(s): D6VRJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3810 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.