Reviewed, UniProtKB/Swiss-Prot Q12505 (SKS1_YEAST)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase SKS1
    EC=2.7.11.1
Alternative name(s):
    Suppressor kinase of SNF3

Gene names

Name:	SKS1
Synonyms:	SHA3
Ordered Locus Names:	YPL026C
ORF Names:	LPB5

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a role in glucose regulation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Miscellaneous	Present with 2810 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Ref.1 Inferred from genetic interaction. Source: SGD positive regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from genetic interaction. Source: SGD protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to glucose stimulus Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERG10	P41338	1	EBI-17297,EBI-19240

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Serine/threonine-protein kinase SKS1		PRO_0000086657
Regions
	Domain	10 – 338	329	Protein kinase
	Nucleotide binding	16 – 24	9	ATP By similarity
Sites
	Active site	186	1	Proton acceptor By similarity
	Binding site	39	1	ATP
Experimental info
	Mutagenesis	39	1	K → R: Loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	Q12505-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B106D084BAFA61E5	FASTA	502	57,844
	10 20 30 40 50 60 MLSDCLLNNF RITAQIGSGA YGLVFHVVDI LTSREYAVKT VFKSSSMDEF YNKNGLNNNS 70 80 90 100 110 120 QVARTTLLQT QLYHFFKSFQ KKLFLPSVDL DSILQLTENE LNRLPHYREI AFQLRVQSHG 130 140 150 160 170 180 NIVKIHQVLE SSIATFIVMD YYDRDLFTSI VDDKHFVNHG ILIKKVFLQL CSALDHCHRL 190 200 210 220 230 240 GIYHCDIKPE NVLLDRNDNA YLCDFGLSTK SKYLAPNVCV GSSYYMAPER ILYCLNTTTN 250 260 270 280 290 300 GIHVDECCSS LPTDTGDIWS LGIILINLTC IRNPWLKAHQ KEDNTFQHFA NDNNVLKKIL 310 320 330 340 350 360 PISDELFTVL TKILQLNPYT RIDMKTLMSE VSSLTSFTRE GPLSQVPILS SEVYMTHIIR 370 380 390 400 410 420 NENLFLSDLS HFSADQEQQQ QQQQQQQQVQ EQEQEQKQEQ IQNQEQAQQQ QEEEDAEPES 430 440 450 460 470 480 DIPSTYNSDG SMEKYEYTNN HNNSTFLTSS MDSTPYQSDI DDVSASKDCK FQQDTLRNRL 490 500 LCLQMNFSTL TDGPNEKWLP DY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SKS1 protein kinase is a multicopy suppressor of the snf3 mutation of Saccharomyces cerevisiae." Yang Z., Bisson L.F. Yeast 12:1407-1419(1996) [PubMed: 8948096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30613 Genomic DNA. Translation: AAC49570.1. U36624 Genomic DNA. Translation: AAB68161.1.
PIR	S61935.
RefSeq	NP_015299.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2879N.
IntAct	Q12505. 14 interactions.
STRING	Q12505.
Genome annotation databases
Ensembl	YPL026C; YPL026C; YPL026C; Saccharomyces cerevisiae. [Genome view]
GeneID	856081.
KEGG	sce:YPL026C.
NMPDR	fig|4932.3.peg.6433.
Organism-specific databases
CYGD	YPL026c.
SGD	S000005947. SKS1.
Phylogenomic databases
HOGENOM	Q12505.
OMA	IATFIVM
OrthoDB	EOG9X6DRH
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	Q12505.
Genevestigator	Q12505.
GermOnline	YPL026C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981092.

Entry information

Entry name

SKS1_YEAST

Accession

Primary (citable) accession number: Q12505

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)