Q124G2 (ACEK_POLSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Bpro_4188

Organism

Polaromonas sp. (strain JS666 / ATCC BAA-500) [Complete proteome] [HAMAP]

Taxonomic identifier

296591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Polaromonas

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000259152
Regions
	Nucleotide binding	325 – 331	7	ATP By similarity
Sites
	Active site	381	1	By similarity
	Binding site	346	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q124G2 [UniParc]. Last modified August 22, 2006. Version 1. Checksum: 2F2483C07E0BD43F	FASTA	625	72,902
	10 20 30 40 50 60 MFPQRLDSTI AYDIAKAMMD GFNRHYQLFR TESARAKHRF ETADWHGQQR AQRERIEFYD 70 80 90 100 110 120 LRVREASTRL EREFKAGEQS MDVWHQVKLH YIGLLVNHHQ PELAETFFNS VTTKILHRSY 130 140 150 160 170 180 FQNDFIFVRP AVSTEYIENE EPTAQPTYRA YYPTRDNLRE IIVRLVRDFD LRLEFEDLER 190 200 210 220 230 240 DAGYVLEAVS ERLSDVKLRA NFQIQVLSGL FFRNKGAYVV GKIINGFNEV PLALPILHSK 250 260 270 280 290 300 SGKLVIDAAL FGEDDLLILF SFARAYFMVD MGIPSAYVQF LRSMMPHMPR AEIYNALGLA 310 320 330 340 350 360 KQGKTLFYRD FLHHLRHSTD KFRIAPGIKG MVMLVFDLPS FPYVFKVIKD YYPPQKDTSR 370 380 390 400 410 420 EQIKGKYLLV KQHDRVGRMA DTQEYSEVAF PRARFDDELI AEIEKFAPSQ LEISDRDRDG 430 440 450 460 470 480 NTEVIIKHVY IERRMIPLNI YLQEAFDAGV GEPAAKNQIE RAVVEYGNAI KDLVAANIFP 490 500 510 520 530 540 GDMLWKNFGI TRHGKVVFYD YDEIEYITDC KFRRVPAPRN DEEEMSGEVW YSVGPKDVFP 550 560 570 580 590 600 ETFAPFLLGN DAVREVFMKH HGDLLDAEFW QSHQARIQAG HVHDVFPYER EKRFTRRHSP 610 620 GRNDHELLTH LPPEPMLTGL SGMTP

References

[1]

"Complete sequence of chromosome of Polaromonas sp. JS666." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Munk A.C., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Anderson I., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JS666 / ATCC BAA-500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000316 Genomic DNA. Translation: ABE46080.1.
RefSeq	YP_550978.1. NC_007948.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q124G2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4013128.
GenomeReviews	Gene locus Bpro_4188 in contig CP000316_GR.
KEGG	pol:Bpro_4188.
NMPDR	fig|296591.1.peg.4260.
PATRIC	22961622. VBIPolSp102244_4264.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q124G2.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	PSP296591:BPRO_4188-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_POLSJ

Accession

Primary (citable) accession number: Q124G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	August 22, 2006
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program