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Protein

Nucleolar protein 58

Gene

NOP58

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-18S rRNA processing. May bind microtubules.2 Publications

GO - Molecular functioni

  • snoRNA binding Source: GO_Central
  • snRNA binding Source: UniProtKB

GO - Biological processi

  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • rRNA 2'-O-methylation Source: Reactome
  • rRNA methylation Source: Reactome
  • rRNA modification Source: GO_Central
  • rRNA processing Source: UniProtKB
  • snRNP protein import into nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33793-MONOMER.
ReactomeiR-SCE-4570464. SUMOylation of RNA binding proteins.
R-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 58
Alternative name(s):
Nucleolar protein 5
Gene namesi
Name:NOP58
Synonyms:NOP5
Ordered Locus Names:YOR310C
ORF Names:O6108
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR310C.
SGDiS000005837. NOP58.

Subcellular locationi

GO - Cellular componenti

  • box C/D snoRNP complex Source: SGD
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: Reactome
  • small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190291 – 511Nucleolar protein 58Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki281Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ12499.
PRIDEiQ12499.

PTM databases

iPTMnetiQ12499.

Interactioni

Subunit structurei

Interacts with SIK1/NOP56 and NOP1. Interacts with the trimethylguanosine synthase TGS1. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NOP1P156464EBI-12126,EBI-6838

Protein-protein interaction databases

BioGridi34698. 202 interactors.
DIPiDIP-5843N.
IntActiQ12499. 83 interactors.
MINTiMINT-658431.

Structurei

3D structure databases

ProteinModelPortaliQ12499.
SMRiQ12499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini283 – 403NopPROSITE-ProRule annotationAdd BLAST121

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili435 – 511Sequence analysisAdd BLAST77

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi441 – 511Asp/Glu/Lys-richAdd BLAST71

Sequence similaritiesi

Belongs to the NOP5/NOP56 family.Curated
Contains 1 Nop domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074998.
HOGENOMiHOG000196310.
InParanoidiQ12499.
KOiK14565.
OMAiVAYEHAP.
OrthoDBiEOG092C3GG0.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12499-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYVLTETSA GYALLKASDK KIYKSSSLIQ DLDSSDKVLK EFKIAAFSKF
60 70 80 90 100
NSAANALEEA NSIIEGKVSS QLEKLLEEIK KDKKSTLIVS ETKLANAINK
110 120 130 140 150
LGLNFNVVSD AVTLDIYRAI KEYLPELLPG MSDNDLSKMS LGLAHSIGRH
160 170 180 190 200
KLKFSADKVD VMIIQAIALL DDLDKELNTY AMRCKEWYGW HFPELAKIVT
210 220 230 240 250
DSVAYARIIL TMGIRSKASE TDLSEILPEE IEERVKTAAE VSMGTEITQT
260 270 280 290 300
DLDNINALAE QIVEFAAYRE QLSNYLSARM KAIAPNLTQL VGELVGARLI
310 320 330 340 350
AHSGSLISLA KSPASTIQIL GAEKALFRAL KTKHDTPKYG LLYHASLVGQ
360 370 380 390 400
ATGKNKGKIA RVLAAKAAVS LRYDALAEDR DDSGDIGLES RAKVENRLSQ
410 420 430 440 450
LEGRDLRTTP KVVREAKKVE MTEARAYNAD ADTAKAASDS ESDSDDEEEE
460 470 480 490 500
KKEKKEKKRK RDDDEDSKDS KKAKKEKKDK KEKKEKKEKK EKKEKKEKKE
510
KKSKKEKKEK K
Length:511
Mass (Da):56,956
Last modified:November 1, 1996 - v1
Checksum:i8A2889448B2A19E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056070 Genomic DNA. Translation: AAC39484.1.
X90565 Genomic DNA. Translation: CAA62165.1.
Z75217 Genomic DNA. Translation: CAA99630.1.
BK006948 Genomic DNA. Translation: DAA11075.1.
PIRiS58322.
RefSeqiNP_014955.1. NM_001183730.1.

Genome annotation databases

EnsemblFungiiYOR310C; YOR310C; YOR310C.
GeneIDi854487.
KEGGisce:YOR310C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056070 Genomic DNA. Translation: AAC39484.1.
X90565 Genomic DNA. Translation: CAA62165.1.
Z75217 Genomic DNA. Translation: CAA99630.1.
BK006948 Genomic DNA. Translation: DAA11075.1.
PIRiS58322.
RefSeqiNP_014955.1. NM_001183730.1.

3D structure databases

ProteinModelPortaliQ12499.
SMRiQ12499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34698. 202 interactors.
DIPiDIP-5843N.
IntActiQ12499. 83 interactors.
MINTiMINT-658431.

PTM databases

iPTMnetiQ12499.

Proteomic databases

MaxQBiQ12499.
PRIDEiQ12499.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR310C; YOR310C; YOR310C.
GeneIDi854487.
KEGGisce:YOR310C.

Organism-specific databases

EuPathDBiFungiDB:YOR310C.
SGDiS000005837. NOP58.

Phylogenomic databases

GeneTreeiENSGT00550000074998.
HOGENOMiHOG000196310.
InParanoidiQ12499.
KOiK14565.
OMAiVAYEHAP.
OrthoDBiEOG092C3GG0.

Enzyme and pathway databases

BioCyciYEAST:G3O-33793-MONOMER.
ReactomeiR-SCE-4570464. SUMOylation of RNA binding proteins.
R-SCE-6790901. rRNA modification in the nucleus and cytosol.
R-SCE-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

PROiQ12499.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOP58_YEAST
AccessioniPrimary (citable) accession number: Q12499
Secondary accession number(s): D6W309
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 34700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.