Q12499 (NOP58_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar protein 58

Alternative name(s):
Nucleolar protein 5

Gene names

Name:	NOP58
Synonyms:	NOP5
Ordered Locus Names:	YOR310C
ORF Names:	O6108

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for pre-18S rRNA processing. May bind microtubules. Ref.2 Ref.5
Subunit structure	Interacts with SIK1/NOP56 and NOP1. Interacts with the trimethylguanosine synthase TGS1. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.5 Ref.6
Subcellular location	Nucleus › nucleolus Ref.2.
Miscellaneous	Present with 34700 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the NOP5/NOP56 family. Contains 1 Nop domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD snRNP protein import into nucleus Non-traceable author statement Ref.2. Source: UniProtKB
Cellular component	90S preribosome Inferred from direct assay. Source: SGD box C/D snoRNP complex Inferred from direct assay. Source: SGD small-subunit processome Inferred from direct assay Ref.7. Source: SGD
Molecular function	snRNA binding Inferred from physical interaction Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Nucleolar protein 58		PRO_0000219029
Regions
	Domain	254 – 404	151	Nop
	Coiled coil	435 – 511	77	Potential
	Compositional bias	441 – 511	71	Asp/Glu/Lys-rich
Amino acid modifications
	Modified residue	399	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q12499 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8A2889448B2A19E2	FASTA	511	56,956
	10 20 30 40 50 60 MAYVLTETSA GYALLKASDK KIYKSSSLIQ DLDSSDKVLK EFKIAAFSKF NSAANALEEA 70 80 90 100 110 120 NSIIEGKVSS QLEKLLEEIK KDKKSTLIVS ETKLANAINK LGLNFNVVSD AVTLDIYRAI 130 140 150 160 170 180 KEYLPELLPG MSDNDLSKMS LGLAHSIGRH KLKFSADKVD VMIIQAIALL DDLDKELNTY 190 200 210 220 230 240 AMRCKEWYGW HFPELAKIVT DSVAYARIIL TMGIRSKASE TDLSEILPEE IEERVKTAAE 250 260 270 280 290 300 VSMGTEITQT DLDNINALAE QIVEFAAYRE QLSNYLSARM KAIAPNLTQL VGELVGARLI 310 320 330 340 350 360 AHSGSLISLA KSPASTIQIL GAEKALFRAL KTKHDTPKYG LLYHASLVGQ ATGKNKGKIA 370 380 390 400 410 420 RVLAAKAAVS LRYDALAEDR DDSGDIGLES RAKVENRLSQ LEGRDLRTTP KVVREAKKVE 430 440 450 460 470 480 MTEARAYNAD ADTAKAASDS ESDSDDEEEE KKEKKEKKRK RDDDEDSKDS KKAKKEKKDK 490 500 510 KEKKEKKEKK EKKEKKEKKE KKSKKEKKEK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII." Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M. Yeast 12:1021-1031(1996) [PubMed: 8896266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Nop5p is a small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast." Wu P., Brockenbrough J.S., Metcalfe A.C., Chen S., Aris J.P. J. Biol. Chem. 273:16453-16463(1998) [PubMed: 9632712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: ATCC 200060 / W303.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis." Gautier T., Berges T., Tollervey D., Hurt E. Mol. Cell. Biol. 17:7088-7098(1997) [PubMed: 9372940] [Abstract] Cited for: FUNCTION, INTERACTION WITH NOP1 AND SIK1.
[6]	"Hypermethylation of the cap structure of both yeast snRNAs and snoRNAs requires a conserved methyltransferase that is localized to the nucleolus." Mouaikel J., Verheggen C., Bertrand E., Tazi J., Bordonne R. Mol. Cell 9:891-901(2002) [PubMed: 11983179] [Abstract] Cited for: INTERACTION WITH TGS1.
[7]	"A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis." Dragon F., Gallagher J.E.G., Compagnone-Post P.A., Mitchell B.M., Porwancher K.A., Wehner K.A., Wormsley S., Settlage R.E., Shabanowitz J., Osheim Y., Beyer A.L., Hunt D.F., Baserga S.J. Nature 417:967-970(2002) [PubMed: 12068309] [Abstract] Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-399, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF056070 Genomic DNA. Translation: AAC39484.1. X90565 Genomic DNA. Translation: CAA62165.1. Z75217 Genomic DNA. Translation: CAA99630.1. BK006948 Genomic DNA. Translation: DAA11075.1.
PIR	S58322.
RefSeq	NP_014955.1. NM_001183730.1.
3D structure databases
ProteinModelPortal	Q12499.
SMR	Q12499. Positions 4-404.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5843N.
IntAct	Q12499. 74 interactions.
MINT	MINT-658431.
STRING	Q12499.
Proteomic databases
PeptideAtlas	Q12499.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR310C; YOR310C; YOR310C.
GeneID	854487.
KEGG	sce:YOR310C.
NMPDR	fig|4932.3.peg.6068.
Organism-specific databases
CYGD	YOR310c.
SGD	S000005837. NOP58.
Phylogenomic databases
eggNOG	fuNOG04650.
GeneTree	EFGT00050000000030.
HOGENOM	HBG611715.
OMA	SGDIGLE.
OrthoDB	EOG4BZRB2.
Gene expression databases
ArrayExpress	Q12499.
Genevestigator	Q12499.
GermOnline	YOR310C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012974. NOP5_N. IPR012976. NOSIC. IPR002687. SnoRNA-bd_dom. [Graphical view]
KO	K14565.
Pfam	PF01798. Nop. 1 hit. PF08156. NOP5NT. 1 hit. PF08060. NOSIC. 1 hit. [Graphical view]
SMART	SM00931. NOSIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976810.

Entry information

Entry name

NOP58_YEAST

Accession

Primary (citable) accession number: Q12499
Secondary accession number(s): D6W309

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program