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Protein

Chromatin assembly factor 1 subunit p90

Gene

RLF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p90 may facilitate the efficient and timely assembly of histones into telomeric chromatin.

GO - Biological processi

  • DNA replication-dependent nucleosome assembly Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-34178-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin assembly factor 1 subunit p90
Alternative name(s):
CAF-1 90 kDa subunit
RAP1 localization factor 2
Gene namesi
Name:RLF2
Synonyms:CAC1
Ordered Locus Names:YPR018W
ORF Names:YP9531.12
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR018W.
SGDiS000006222. RLF2.

Subcellular locationi

GO - Cellular componenti

  • CAF-1 complex Source: SGD
  • chromosome, centromeric region Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 606606Chromatin assembly factor 1 subunit p90PRO_0000089272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphothreonineCombined sources
Modified residuei94 – 941PhosphoserineCombined sources
Modified residuei509 – 5091PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12495.
PRIDEiQ12495.

PTM databases

iPTMnetiQ12495.

Interactioni

Subunit structurei

Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and RLF2/CAC1/p90. RLF2 interacts with SAS2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MSI1P137124EBI-3913,EBI-11391
ORC3P547903EBI-3913,EBI-12576
SAS2P409634EBI-3913,EBI-16476
SAS4Q040033EBI-3913,EBI-38500

Protein-protein interaction databases

BioGridi36195. 192 interactions.
DIPiDIP-923N.
IntActiQ12495. 145 interactions.
MINTiMINT-386233.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5EJOX-ray2.75A519-606[»]
ProteinModelPortaliQ12495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili128 – 240113Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi122 – 236115Arg/Glu/Lys-rich (highly charged)Add
BLAST
Compositional biasi166 – 1694Poly-Lys
Compositional biasi383 – 43654Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi393 – 3986Poly-Glu
Compositional biasi406 – 4127Poly-Glu

Sequence similaritiesi

Belongs to the RLF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000000996.
InParanoidiQ12495.
KOiK10750.
OMAiPPIKYIS.
OrthoDBiEOG73NGCV.

Family and domain databases

InterProiIPR022043. CAF1A.
[Graphical view]
PfamiPF12253. CAF1A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12495-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQHLKSIPL QDDTKKKGIL SFFQNTTTVK SNKFLTKEKD VITLDDPKED
60 70 80 90 100
VSGPMIETVK QETMKSINKE CADEMKTTPK KANAEDKLLC YKNSPIQSTK
110 120 130 140 150
YDRNTNKQVP NGNIIAIETK SRSSSPCSKR ELSSSKKEEA KREKELKKQQ
160 170 180 190 200
RAEEKHRKEL LRQEEKKKKE LKVEEERQRR AELKKQKEEE KRRKEEARLE
210 220 230 240 250
AKRRKEEERL KKEEEIRLKE EAKERAQSRI GNFFKKLSDS NTPVVEKSDY
260 270 280 290 300
EKFFLPFYAK DGVRVSNKWK LTKVELEGSK RKIDDELLNS KDKTSSDDLL
310 320 330 340 350
NWLQSRRLPR GHKIKRKAVD VLQQMPLKEK TDDELQSLLA QVPHKYIKFY
360 370 380 390 400
ENVRPPFIGT YSMDFTLPPN DPFSTKGTGF NYDYDSDVEW VNEEEEGEVD
410 420 430 440 450
NLESGEEEEE EEDDEDVPSE GEFDGFLDSE ENSDLDGLPC AKRKFVGPLI
460 470 480 490 500
PTICLKSNFE NLSEENKRYL QQLKAEVIIE TDGPIDPFKE PKTSSLPSKR
510 520 530 540 550
SNSDLQAQTA SQSQSPEKKQ KAMITDPMDL LRLFDGVQDS TFSLGTVTEI
560 570 580 590 600
AQKNLPQYNK QTIKNTIKEY AIRSSGKGDL PRKWVIKDAQ NWENLRANAN

MPTPSL
Length:606
Mass (Da):70,210
Last modified:November 1, 1996 - v1
Checksum:iC593E6E4FE5FD778
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49919 Genomic DNA. Translation: CAA90163.1.
Z71255 Genomic DNA. Translation: CAA95014.1.
BK006949 Genomic DNA. Translation: DAA11444.1.
PIRiS57552.
RefSeqiNP_015343.1. NM_001184115.1.

Genome annotation databases

EnsemblFungiiYPR018W; YPR018W; YPR018W.
GeneIDi856129.
KEGGisce:YPR018W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49919 Genomic DNA. Translation: CAA90163.1.
Z71255 Genomic DNA. Translation: CAA95014.1.
BK006949 Genomic DNA. Translation: DAA11444.1.
PIRiS57552.
RefSeqiNP_015343.1. NM_001184115.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5EJOX-ray2.75A519-606[»]
ProteinModelPortaliQ12495.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36195. 192 interactions.
DIPiDIP-923N.
IntActiQ12495. 145 interactions.
MINTiMINT-386233.

PTM databases

iPTMnetiQ12495.

Proteomic databases

MaxQBiQ12495.
PRIDEiQ12495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR018W; YPR018W; YPR018W.
GeneIDi856129.
KEGGisce:YPR018W.

Organism-specific databases

EuPathDBiFungiDB:YPR018W.
SGDiS000006222. RLF2.

Phylogenomic databases

HOGENOMiHOG000000996.
InParanoidiQ12495.
KOiK10750.
OMAiPPIKYIS.
OrthoDBiEOG73NGCV.

Enzyme and pathway databases

BioCyciYEAST:G3O-34178-MONOMER.

Miscellaneous databases

NextBioi981218.
PROiQ12495.

Family and domain databases

InterProiIPR022043. CAF1A.
[Graphical view]
PfamiPF12253. CAF1A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo."
    Enomoto S., McCune-Zierath P.D., Gerami-Nejad M., Sanders M.A., Berman J.
    Genes Dev. 11:358-370(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I."
    Kaufman P.D., Kobayashi R., Stillman B.
    Genes Dev. 11:345-357(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 237-247 AND 554-560, CHARACTERIZATION.
  5. "The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae."
    Meijsing S.H., Ehrenhofer-Murray A.E.
    Genes Dev. 15:3169-3182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SAS2.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-94, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-78; SER-94 AND THR-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRLF2_YEAST
AccessioniPrimary (citable) accession number: Q12495
Secondary accession number(s): D6W428
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.