Reviewed, UniProtKB/Swiss-Prot Q12495 (RLF2_YEAST)

Names and origin

Protein names

Recommended name:
    Chromatin assembly factor 1 subunit p90
Alternative name(s):
    CAF-1 90 kDa subunit
    RAP1 localization factor 2

Gene names

Name:	RLF2
Synonyms:	CAC1
Ordered Locus Names:	YPR018W
ORF Names:	YP9531.12

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p90 may facilitate the efficient and timely assembly of histones into telomeric chromatin.
Subunit structure	Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and RLF2/CAC1/p90. RLF2 interacts with SAS2. Ref.4
Subcellular location	Nucleus.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the RLF2 family.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication-dependent nucleosome assembly Ref.1 Inferred from mutant phenotype. Source: SGD chromatin silencing at silent mating-type cassette Inferred from genetic interaction. Source: SGD
Cellular component	CAF-1 complex Ref.3 Inferred from direct assay. Source: SGD chromosome, centromeric region Inferred from direct assay. Source: SGD
Molecular function	chromatin binding Inferred from genetic interaction. Source: SGD protein binding Ref.4 Inferred from physical interaction. Source: IntAct transcription regulator activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CAC2	Q04199	1	EBI-3913,EBI-3920
ORC3	P54790	2	EBI-3913,EBI-12576
SAS2	P40963	4	EBI-3913,EBI-16476
SAS4	Q04003	1	EBI-3913,EBI-38500

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Chromatin assembly factor 1 subunit p90		PRO_0000089272
Regions
	Coiled coil	128 – 240	113	Potential
	Compositional bias	122 – 236	115	Arg/Glu/Lys-rich (highly charged)
	Compositional bias	166 – 169	4	Poly-Lys
	Compositional bias	383 – 436	54	Asp/Glu-rich (acidic)
	Compositional bias	393 – 398	6	Poly-Glu
	Compositional bias	406 – 412	7	Poly-Glu
Amino acid modifications
	Modified residue	78	1	Phosphothreonine Ref.7
	Modified residue	91	1	Phosphotyrosine Ref.7
	Modified residue	94	1	Phosphoserine Ref.7
	Modified residue	279	1	Phosphoserine Ref.7
	Modified residue	501	1	Phosphoserine Ref.6
	Modified residue	503	1	Phosphoserine Ref.7 Ref.6
	Modified residue	515	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q12495-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C593E6E4FE5FD778	FASTA	606	70,210
	10 20 30 40 50 60 MEQHLKSIPL QDDTKKKGIL SFFQNTTTVK SNKFLTKEKD VITLDDPKED VSGPMIETVK 70 80 90 100 110 120 QETMKSINKE CADEMKTTPK KANAEDKLLC YKNSPIQSTK YDRNTNKQVP NGNIIAIETK 130 140 150 160 170 180 SRSSSPCSKR ELSSSKKEEA KREKELKKQQ RAEEKHRKEL LRQEEKKKKE LKVEEERQRR 190 200 210 220 230 240 AELKKQKEEE KRRKEEARLE AKRRKEEERL KKEEEIRLKE EAKERAQSRI GNFFKKLSDS 250 260 270 280 290 300 NTPVVEKSDY EKFFLPFYAK DGVRVSNKWK LTKVELEGSK RKIDDELLNS KDKTSSDDLL 310 320 330 340 350 360 NWLQSRRLPR GHKIKRKAVD VLQQMPLKEK TDDELQSLLA QVPHKYIKFY ENVRPPFIGT 370 380 390 400 410 420 YSMDFTLPPN DPFSTKGTGF NYDYDSDVEW VNEEEEGEVD NLESGEEEEE EEDDEDVPSE 430 440 450 460 470 480 GEFDGFLDSE ENSDLDGLPC AKRKFVGPLI PTICLKSNFE NLSEENKRYL QQLKAEVIIE 490 500 510 520 530 540 TDGPIDPFKE PKTSSLPSKR SNSDLQAQTA SQSQSPEKKQ KAMITDPMDL LRLFDGVQDS 550 560 570 580 590 600 TFSLGTVTEI AQKNLPQYNK QTIKNTIKEY AIRSSGKGDL PRKWVIKDAQ NWENLRANAN MPTPSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo." Enomoto S., McCune-Zierath P.D., Gerami-Nejad M., Sanders M.A., Berman J. Genes Dev. 11:358-370(1997) [PubMed: 9030688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I." Kaufman P.D., Kobayashi R., Stillman B. Genes Dev. 11:345-357(1997) [PubMed: 9030687] [Abstract] Cited for: PROTEIN SEQUENCE OF 237-247 AND 554-560, CHARACTERIZATION.
[4]	"The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae." Meijsing S.H., Ehrenhofer-Murray A.E. Genes Dev. 15:3169-3182(2001) [PubMed: 11731480] [Abstract] Cited for: INTERACTION WITH SAS2.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501; SER-503 AND SER-515, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-78; TYR-91; SER-94; SER-279 AND SER-503, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49919 Genomic DNA. Translation: CAA90163.1. Z71255 Genomic DNA. Translation: CAA95014.1.
PIR	S57552.
RefSeq	NP_015343.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:923N.
IntAct	Q12495. 13 interactions.
STRING	Q12495.
Genome annotation databases
Ensembl	YPR018W; YPR018W; YPR018W; Saccharomyces cerevisiae. [Genome view]
GeneID	856129.
KEGG	sce:YPR018W.
NMPDR	fig|4932.3.peg.6478.
Organism-specific databases
CYGD	YPR018w.
SGD	S000006222. RLF2.
Phylogenomic databases
OMA	YENVRPP
OrthoDB	EOG9N05XC
Gene expression databases
ArrayExpress	Q12495.
Genevestigator	Q12495.
GermOnline	YPR018W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	981218.

Entry information

Entry name

RLF2_YEAST

Accession

Primary (citable) accession number: Q12495

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)