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Reviewed, UniProtKB/Swiss-Prot Q12495 (RLF2_YEAST)

Last modified November 24, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Chromatin assembly factor 1 subunit p90
Alternative name(s):
    CAF-1 90 kDa subunit
    RAP1 localization factor 2
Gene names
Name: RLF2
Synonyms: CAC1
Ordered Locus Names: YPR018W
ORF Names: YP9531.12
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a component of chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto replicating DNA in vitro. It performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. p90 may facilitate the efficient and timely assembly of histones into telomeric chromatin.

Subunit structure

Component of chromatin assembly factor 1 (CAF-1), which is composed of MSI1/p50, CAC2/p60 and RLF2/CAC1/p90. RLF2 interacts with SAS2. Ref.4

Subcellular location

Nucleus.

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the RLF2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606Chromatin assembly factor 1 subunit p90
PRO_0000089272

Regions

Coiled coil128 – 240113 Potential
Compositional bias122 – 236115Arg/Glu/Lys-rich (highly charged)
Compositional bias166 – 1694Poly-Lys
Compositional bias383 – 43654Asp/Glu-rich (acidic)
Compositional bias393 – 3986Poly-Glu
Compositional bias406 – 4127Poly-Glu

Amino acid modifications

Modified residue781Phosphothreonine Ref.7
Modified residue911Phosphotyrosine Ref.7
Modified residue941Phosphoserine Ref.7
Modified residue2791Phosphoserine Ref.7
Modified residue5011Phosphoserine Ref.6
Modified residue5031Phosphoserine Ref.7 Ref.6
Modified residue5151Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q12495-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: C593E6E4FE5FD778

FASTA60670,210
        10         20         30         40         50         60 
MEQHLKSIPL QDDTKKKGIL SFFQNTTTVK SNKFLTKEKD VITLDDPKED VSGPMIETVK 

        70         80         90        100        110        120 
QETMKSINKE CADEMKTTPK KANAEDKLLC YKNSPIQSTK YDRNTNKQVP NGNIIAIETK 

       130        140        150        160        170        180 
SRSSSPCSKR ELSSSKKEEA KREKELKKQQ RAEEKHRKEL LRQEEKKKKE LKVEEERQRR 

       190        200        210        220        230        240 
AELKKQKEEE KRRKEEARLE AKRRKEEERL KKEEEIRLKE EAKERAQSRI GNFFKKLSDS 

       250        260        270        280        290        300 
NTPVVEKSDY EKFFLPFYAK DGVRVSNKWK LTKVELEGSK RKIDDELLNS KDKTSSDDLL 

       310        320        330        340        350        360 
NWLQSRRLPR GHKIKRKAVD VLQQMPLKEK TDDELQSLLA QVPHKYIKFY ENVRPPFIGT 

       370        380        390        400        410        420 
YSMDFTLPPN DPFSTKGTGF NYDYDSDVEW VNEEEEGEVD NLESGEEEEE EEDDEDVPSE 

       430        440        450        460        470        480 
GEFDGFLDSE ENSDLDGLPC AKRKFVGPLI PTICLKSNFE NLSEENKRYL QQLKAEVIIE 

       490        500        510        520        530        540 
TDGPIDPFKE PKTSSLPSKR SNSDLQAQTA SQSQSPEKKQ KAMITDPMDL LRLFDGVQDS 

       550        560        570        580        590        600 
TFSLGTVTEI AQKNLPQYNK QTIKNTIKEY AIRSSGKGDL PRKWVIKDAQ NWENLRANAN 


MPTPSL 

« Hide

References

« Hide 'large scale' references
[1]"RLF2, a subunit of yeast chromatin assembly factor-I, is required for telomeric chromatin function in vivo."
Enomoto S., McCune-Zierath P.D., Gerami-Nejad M., Sanders M.A., Berman J.
Genes Dev. 11:358-370(1997) [PubMed: 9030688] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Ultraviolet radiation sensitivity and reduction of telomeric silencing in Saccharomyces cerevisiae cells lacking chromatin assembly factor-I."
Kaufman P.D., Kobayashi R., Stillman B.
Genes Dev. 11:345-357(1997) [PubMed: 9030687] [Abstract]
Cited for: PROTEIN SEQUENCE OF 237-247 AND 554-560, CHARACTERIZATION.
[4]"The silencing complex SAS-I links histone acetylation to the assembly of repressed chromatin by CAF-I and Asf1 in Saccharomyces cerevisiae."
Meijsing S.H., Ehrenhofer-Murray A.E.
Genes Dev. 15:3169-3182(2001) [PubMed: 11731480] [Abstract]
Cited for: INTERACTION WITH SAS2.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501; SER-503 AND SER-515, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-78; TYR-91; SER-94; SER-279 AND SER-503, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z49919 Genomic DNA. Translation: CAA90163.1.
Z71255 Genomic DNA. Translation: CAA95014.1.
PIRS57552.
RefSeqNP_015343.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:923N.
IntActQ12495. 13 interactions.
STRINGQ12495.

Genome annotation databases

EnsemblYPR018W; YPR018W; YPR018W; Saccharomyces cerevisiae. [Genome view]
GeneID856129.
KEGGsce:YPR018W.
NMPDRfig|4932.3.peg.6478.

Organism-specific databases

CYGDYPR018w.
SGDS000006222. RLF2.

Phylogenomic databases

OMAYENVRPP
OrthoDBEOG9N05XC

Gene expression databases

ArrayExpressQ12495.
GenevestigatorQ12495.
GermOnlineYPR018W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio981218.

Entry information

Entry nameRLF2_YEAST
AccessionPrimary (citable) accession number: Q12495
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents