ID   NKP1_YEAST              Reviewed;         238 AA.
AC   Q12493;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 2.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Central kinetochore subunit NKP1;
DE   AltName: Full=Non-essential kinetochore protein 1;
GN   Name=NKP1; OrderedLocusNames=YDR383C;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=97313263; PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
RA   Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
RA   Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
RA   Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
RA   Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
RA   Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
RA   Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
RA   Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
RA   Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
RA   Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
RA   Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
RA   Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
RA   Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
RA   Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
RA   Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
RA   Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
RA   Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
RA   Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
RA   Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
RA   Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
RA   Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
RA   Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [2]
RP   IDENTIFICATION OF PROBABLE INITIATION SITE.
RX   MEDLINE=22633889; PubMed=12748633; DOI=10.1038/nature01644;
RA   Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT   "Sequencing and comparison of yeast species to identify genes and
RT   regulatory elements.";
RL   Nature 423:241-254(2003).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CENTRAL KINETOCHORE
RP   COMPLEX, AND SUBCELLULAR LOCATION.
RX   MEDLINE=22296671; PubMed=12408861; DOI=10.1016/S0092-8674(02)00973-X;
RA   Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S.,
RA   Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.;
RT   "Phospho-regulation of kinetochore-microtubule attachments by the
RT   Aurora kinase Ipl1p.";
RL   Cell 111:163-172(2002).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-222, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Component of the central kinetochore, which mediates the
CC       attachment of the centromere to the mitotic spindle by forming
CC       essential interactions between the microtubule-associated outer
CC       kinetochore proteins and the centromere-associated inner
CC       kinetochore proteins. Required for establishing bipolar spindle-
CC       microtubule attachments and proper chromosome segregation.
CC   -!- SUBUNIT: Component of the central kinetochore CTF19 complex, which
CC       is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16,
CC       MCM21, MCM22, NKP1, NKP2 and OKP1.
CC   -!- INTERACTION:
CC       Q06162:NKP2; NbExp=1; IntAct=EBI-35840, EBI-34256;
CC       Q02821:SRP1; NbExp=1; IntAct=EBI-35840, EBI-1797;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Associated with
CC       kinetochores.
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DR   EMBL; U28373; AAB64819.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U32274; AAB64825.1; ALT_INIT; Genomic_DNA.
DR   PIR; S61178; S61178.
DR   RefSeq; NP_010671.2; -.
DR   DIP; DIP:1940N; -.
DR   IntAct; Q12493; 15.
DR   Ensembl; YDR383C; Saccharomyces cerevisiae.
DR   GeneID; 851991; -.
DR   GenomeReviews; Z71256_GR; YDR383C.
DR   KEGG; sce:YDR383C; -.
DR   NMPDR; fig|4932.3.peg.1443; -.
DR   CYGD; YDR383c; -.
DR   SGD; S000002791; NKP1.
DR   HOGENOM; Q12493; -.
DR   OMA; Q12493; YELENEW.
DR   NextBio; 970157; -.
DR   GermOnline; YDR383C; Saccharomyces cerevisiae.
DR   GO; GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007126; P:meiosis; IEA:UniProtKB-KW.
DR   GO; GO:0007067; P:mitosis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Complete proteome; Kinetochore; Meiosis;
KW   Mitosis; Nucleus; Phosphoprotein.
FT   CHAIN         1    238       Central kinetochore subunit NKP1.
FT                                /FTId=PRO_0000096867.
FT   MOD_RES     222    222       Phosphoserine.
SQ   SEQUENCE   238 AA;  26976 MW;  A2BE651F72E76B54 CRC64;
     MTDTYNSISN FIENELTALL SSDDYLMDDL AGELPNEVCR LLKAQVIEKR KDAMSRGKQD
     LLSKEIYDNE SELRASQSQQ IMELVGDIPK YSLGSELRNR VEGEPQSTSI ERLIEDVLKL
     PQMEVADEEE VEVENDLKVL SEYSNLRKDL ILKCQALQIG ESKLSDILSQ TNSINSLTTS
     IKEASEDDDI SEYFATYNGK LVVALEEMKL LLEEAVKTFG NSPEKREKIK KILSELKK
//