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Protein

Central kinetochore subunit NKP1

Gene

NKP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-29931-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit NKP1
Alternative name(s):
Non-essential kinetochore protein 1
Gene namesi
Name:NKP1
Ordered Locus Names:YDR383C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR383C.
SGDiS000002791. NKP1.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • kinetochore Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Central kinetochore subunit NKP1PRO_0000096867Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei222 – 2221PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12493.
PRIDEiQ12493.

PTM databases

iPTMnetiQ12493.

Interactioni

Subunit structurei

Component of the central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1.

Binary interactionsi

WithEntry#Exp.IntActNotes
NKP2Q061623EBI-35840,EBI-34256

Protein-protein interaction databases

BioGridi32444. 30 interactions.
DIPiDIP-1940N.
IntActiQ12493. 10 interactions.
MINTiMINT-399404.

Structurei

3D structure databases

ProteinModelPortaliQ12493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

InParanoidiQ12493.
OMAiYELENEW.
OrthoDBiEOG79SF8G.

Sequencei

Sequence statusi: Complete.

Q12493-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTYNSISN FIENELTALL SSDDYLMDDL AGELPNEVCR LLKAQVIEKR
60 70 80 90 100
KDAMSRGKQD LLSKEIYDNE SELRASQSQQ IMELVGDIPK YSLGSELRNR
110 120 130 140 150
VEGEPQSTSI ERLIEDVLKL PQMEVADEEE VEVENDLKVL SEYSNLRKDL
160 170 180 190 200
ILKCQALQIG ESKLSDILSQ TNSINSLTTS IKEASEDDDI SEYFATYNGK
210 220 230
LVVALEEMKL LLEEAVKTFG NSPEKREKIK KILSELKK
Length:238
Mass (Da):26,976
Last modified:September 13, 2005 - v2
Checksum:iA2BE651F72E76B54
GO

Sequence cautioni

The sequence AAB64819.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAB64825.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64819.1. Different initiation.
U32274 Genomic DNA. Translation: AAB64825.1. Different initiation.
BK006938 Genomic DNA. Translation: DAA12227.1.
PIRiS61178.
RefSeqiNP_010671.4. NM_001180691.3.

Genome annotation databases

EnsemblFungiiYDR383C; YDR383C; YDR383C.
GeneIDi851991.
KEGGisce:YDR383C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64819.1. Different initiation.
U32274 Genomic DNA. Translation: AAB64825.1. Different initiation.
BK006938 Genomic DNA. Translation: DAA12227.1.
PIRiS61178.
RefSeqiNP_010671.4. NM_001180691.3.

3D structure databases

ProteinModelPortaliQ12493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32444. 30 interactions.
DIPiDIP-1940N.
IntActiQ12493. 10 interactions.
MINTiMINT-399404.

PTM databases

iPTMnetiQ12493.

Proteomic databases

MaxQBiQ12493.
PRIDEiQ12493.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR383C; YDR383C; YDR383C.
GeneIDi851991.
KEGGisce:YDR383C.

Organism-specific databases

EuPathDBiFungiDB:YDR383C.
SGDiS000002791. NKP1.

Phylogenomic databases

InParanoidiQ12493.
OMAiYELENEW.
OrthoDBiEOG79SF8G.

Enzyme and pathway databases

BioCyciYEAST:G3O-29931-MONOMER.

Miscellaneous databases

PROiQ12493.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  4. "Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
    Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
    Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CENTRAL KINETOCHORE COMPLEX, SUBCELLULAR LOCATION.
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNKP1_YEAST
AccessioniPrimary (citable) accession number: Q12493
Secondary accession number(s): D6VT17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: June 8, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.