Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA replication complex GINS protein PSF1

Gene

PSF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA replication. Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery. Required for the chromatin binding of CDC45.1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • DNA strand elongation involved in mitotic DNA replication Source: GO_Central
  • double-strand break repair via break-induced replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-29632-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein PSF1
Alternative name(s):
Partner of Sld five 1
Gene namesi
Name:PSF1
Ordered Locus Names:YDR013W
ORF Names:PZA208, YD8119.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR013W.
SGDiS000002420. PSF1.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Associates with autonomously replicating sequence (ARS) regions in S phase. This association requires SLD3 and DPB11.

GO - Cellular componenti

  • DNA replication preinitiation complex Source: SGD
  • GINS complex Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi84 – 841R → G in PSF1-1; temperature-sensitive mutant. Defective in DNA replication. Impaired chromatin binding of CDC45. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 208208DNA replication complex GINS protein PSF1PRO_0000219040Add
BLAST

Proteomic databases

MaxQBiQ12488.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.

Binary interactionsi

WithEntry#Exp.IntActNotes
PSF2P403593EBI-22066,EBI-25936
SLD5Q034064EBI-22066,EBI-37437

Protein-protein interaction databases

BioGridi32064. 77 interactions.
DIPiDIP-1812N.
IntActiQ12488. 27 interactions.
MINTiMINT-409391.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JC5electron microscopy4.70A1-206[»]
3JC6electron microscopy3.70A1-208[»]
3JC7electron microscopy4.80A1-206[»]
ProteinModelPortaliQ12488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS1/PSF1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013968.
HOGENOMiHOG000192102.
InParanoidiQ12488.
KOiK10732.
OMAiRKNTEYL.
OrthoDBiEOG7W41QC.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR005339. GINS_Psf1.
[Graphical view]
PANTHERiPTHR12914. PTHR12914. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN
60 70 80 90 100
TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAYQ RLRTDILDSM
110 120 130 140 150
AWNNNGLDLM SSITFSQQDT NNLSHQEQEY LKEYCDLITD LKSGDLVDID
160 170 180 190 200
LSGSLVPPSD VFIDVRVLKD AGEIQTEYGV FNLIKDSQFF VRQSDVERLI

QQGYLQKI
Length:208
Mass (Da):24,204
Last modified:November 1, 1996 - v1
Checksum:i4667D8152C1AB5B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95966 Genomic DNA. Translation: CAA65205.1.
Z74309 Genomic DNA. Translation: CAA98833.1.
Z48008 Genomic DNA. Translation: CAA88073.1.
AY557650 Genomic DNA. Translation: AAS55976.1.
BK006938 Genomic DNA. Translation: DAA11859.1.
PIRiS50994.
RefSeqiNP_010296.3. NM_001180321.3.

Genome annotation databases

EnsemblFungiiYDR013W; YDR013W; YDR013W.
GeneIDi851576.
KEGGisce:YDR013W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95966 Genomic DNA. Translation: CAA65205.1.
Z74309 Genomic DNA. Translation: CAA98833.1.
Z48008 Genomic DNA. Translation: CAA88073.1.
AY557650 Genomic DNA. Translation: AAS55976.1.
BK006938 Genomic DNA. Translation: DAA11859.1.
PIRiS50994.
RefSeqiNP_010296.3. NM_001180321.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JC5electron microscopy4.70A1-206[»]
3JC6electron microscopy3.70A1-208[»]
3JC7electron microscopy4.80A1-206[»]
ProteinModelPortaliQ12488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32064. 77 interactions.
DIPiDIP-1812N.
IntActiQ12488. 27 interactions.
MINTiMINT-409391.

Proteomic databases

MaxQBiQ12488.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR013W; YDR013W; YDR013W.
GeneIDi851576.
KEGGisce:YDR013W.

Organism-specific databases

EuPathDBiFungiDB:YDR013W.
SGDiS000002420. PSF1.

Phylogenomic databases

GeneTreeiENSGT00390000013968.
HOGENOMiHOG000192102.
InParanoidiQ12488.
KOiK10732.
OMAiRKNTEYL.
OrthoDBiEOG7W41QC.

Enzyme and pathway databases

BioCyciYEAST:G3O-29632-MONOMER.

Miscellaneous databases

PROiQ12488.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR005339. GINS_Psf1.
[Graphical view]
PANTHERiPTHR12914. PTHR12914. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
    Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
    Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH DPB11 AND SLD3, COMPOSITION OF THE GINS COMPLEX, SUBCELLULAR LOCATION, MUTAGENESIS OF ARG-84.
  2. "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames."
    Eide L.G., Sander C., Prydz H.
    Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSF1_YEAST
AccessioniPrimary (citable) accession number: Q12488
Secondary accession number(s): D6VRZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1431 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.