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Protein

DNA replication complex GINS protein PSF1

Gene

PSF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for DNA replication. Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery. Required for the chromatin binding of CDC45.1 Publication

Miscellaneous

Present with 1431 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: SGD
  • DNA strand elongation involved in mitotic DNA replication Source: GO_Central
  • double-strand break repair via break-induced replication Source: SGD

Keywordsi

Biological processCell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-29632-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein PSF1
Alternative name(s):
Partner of Sld five 1
Gene namesi
Name:PSF1
Ordered Locus Names:YDR013W
ORF Names:PZA208, YD8119.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR013W
SGDiS000002420 PSF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi84R → G in PSF1-1; temperature-sensitive mutant. Defective in DNA replication. Impaired chromatin binding of CDC45. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190401 – 208DNA replication complex GINS protein PSF1Add BLAST208

Proteomic databases

MaxQBiQ12488
PaxDbiQ12488
PRIDEiQ12488

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3. Interacts with PSF2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32064, 288 interactors
DIPiDIP-1812N
IntActiQ12488, 30 interactors
MINTiQ12488
STRINGi4932.YDR013W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JC5electron microscopy4.70A1-206[»]
3JC6electron microscopy3.70A1-208[»]
3JC7electron microscopy4.80A1-206[»]
5U8Selectron microscopy6.10A1-208[»]
5U8Telectron microscopy4.90A1-208[»]
ProteinModelPortaliQ12488
SMRiQ12488
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS1/PSF1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013968
HOGENOMiHOG000192102
InParanoidiQ12488
KOiK10732
OMAiSMRRNKR
OrthoDBiEOG092C4C04

Family and domain databases

CDDicd11710 GINS_A_psf1, 1 hit
InterProiView protein in InterPro
IPR021151 GINS_A
IPR036224 GINS_bundle-like_dom_sf
IPR005339 GINS_Psf1
PANTHERiPTHR12914 PTHR12914, 1 hit
PfamiView protein in Pfam
PF05916 Sld5, 1 hit
SUPFAMiSSF158573 SSF158573, 1 hit

Sequencei

Sequence statusi: Complete.

Q12488-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN
60 70 80 90 100
TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAYQ RLRTDILDSM
110 120 130 140 150
AWNNNGLDLM SSITFSQQDT NNLSHQEQEY LKEYCDLITD LKSGDLVDID
160 170 180 190 200
LSGSLVPPSD VFIDVRVLKD AGEIQTEYGV FNLIKDSQFF VRQSDVERLI

QQGYLQKI
Length:208
Mass (Da):24,204
Last modified:November 1, 1996 - v1
Checksum:i4667D8152C1AB5B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95966 Genomic DNA Translation: CAA65205.1
Z74309 Genomic DNA Translation: CAA98833.1
Z48008 Genomic DNA Translation: CAA88073.1
AY557650 Genomic DNA Translation: AAS55976.1
BK006938 Genomic DNA Translation: DAA11859.1
PIRiS50994
RefSeqiNP_010296.3, NM_001180321.3

Genome annotation databases

EnsemblFungiiYDR013W; YDR013W; YDR013W
GeneIDi851576
KEGGisce:YDR013W

Similar proteinsi

Entry informationi

Entry nameiPSF1_YEAST
AccessioniPrimary (citable) accession number: Q12488
Secondary accession number(s): D6VRZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health