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Protein

Vacuolar-sorting protein SNF8

Gene

SNF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.1 Publication

GO - Biological processi

  • ATP export Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • protein targeting to vacuole Source: SGD
  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33921-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein SNF8
Alternative name(s):
ESCRT-II complex subunit VPS22
Vacuolar protein-sorting-associated protein 22
Gene namesi
Name:SNF8
Synonyms:VPS22
Ordered Locus Names:YPL002C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL002C.
SGDiS000005923. SNF8.

Subcellular locationi

GO - Cellular componenti

  • ESCRT II complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152141 – 233Vacuolar-sorting protein SNF8Add BLAST233

Proteomic databases

MaxQBiQ12483.
PRIDEiQ12483.

Interactioni

Subunit structurei

Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS25P471424EBI-30277,EBI-25595
VPS36Q066965EBI-30277,EBI-36540

Protein-protein interaction databases

BioGridi36175. 67 interactors.
DIPiDIP-1745N.
IntActiQ12483. 5 interactors.
MINTiMINT-401148.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60A1-233[»]
1W7PX-ray3.60A1-233[»]
ProteinModelPortaliQ12483.
SMRiQ12483.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12483.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 46Sequence analysisAdd BLAST24

Sequence similaritiesi

Belongs to the SNF8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000007843.
HOGENOMiHOG000170930.
InParanoidiQ12483.
KOiK12188.
OMAiQMSKQLE.
OrthoDBiEOG092C4ALW.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR016689. ESCRT-2_cplx_Snf8.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12806. PTHR12806. 1 hit.
PfamiPF04157. EAP30. 1 hit.
[Graphical view]
PIRSFiPIRSF017215. ESCRT2_Vps22. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

Q12483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQFGLAAFD ELKDGKYNDV NKTILEKQSV ELRDQLMVFQ ERLVEFAKKH
60 70 80 90 100
NSELQASPEF RSKFMHMCSS IGIDPLSLFD RDKHLFTVND FYYEVCLKVI
110 120 130 140 150
EICRQTKDMN GGVISFQELE KVHFRKLNVG LDDLEKSIDM LKSLECFEIF
160 170 180 190 200
QIRGKKFLRS VPNELTSDQT KILEICSILG YSSISLLKAN LGWEAVRSKS
210 220 230
ALDEMVANGL LWIDYQGGAE ALYWDPSWIT RQL
Length:233
Mass (Da):26,954
Last modified:November 1, 1996 - v1
Checksum:i212C74F086A0325E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10361 Genomic DNA. Translation: AAA86824.1.
U33335 Genomic DNA. Translation: AAB68103.1.
Z71255 Genomic DNA. Translation: CAA95039.1.
Z48483 Genomic DNA. Translation: CAA88384.1.
AY692759 Genomic DNA. Translation: AAT92778.1.
BK006949 Genomic DNA. Translation: DAA11426.1.
PIRiS52529.
RefSeqiNP_015323.1. NM_001183816.1.

Genome annotation databases

EnsemblFungiiYPL002C; YPL002C; YPL002C.
GeneIDi856105.
KEGGisce:YPL002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10361 Genomic DNA. Translation: AAA86824.1.
U33335 Genomic DNA. Translation: AAB68103.1.
Z71255 Genomic DNA. Translation: CAA95039.1.
Z48483 Genomic DNA. Translation: CAA88384.1.
AY692759 Genomic DNA. Translation: AAT92778.1.
BK006949 Genomic DNA. Translation: DAA11426.1.
PIRiS52529.
RefSeqiNP_015323.1. NM_001183816.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60A1-233[»]
1W7PX-ray3.60A1-233[»]
ProteinModelPortaliQ12483.
SMRiQ12483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36175. 67 interactors.
DIPiDIP-1745N.
IntActiQ12483. 5 interactors.
MINTiMINT-401148.

Proteomic databases

MaxQBiQ12483.
PRIDEiQ12483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL002C; YPL002C; YPL002C.
GeneIDi856105.
KEGGisce:YPL002C.

Organism-specific databases

EuPathDBiFungiDB:YPL002C.
SGDiS000005923. SNF8.

Phylogenomic databases

GeneTreeiENSGT00390000007843.
HOGENOMiHOG000170930.
InParanoidiQ12483.
KOiK12188.
OMAiQMSKQLE.
OrthoDBiEOG092C4ALW.

Enzyme and pathway databases

BioCyciYEAST:G3O-33921-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12483.
PROiQ12483.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR016689. ESCRT-2_cplx_Snf8.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12806. PTHR12806. 1 hit.
PfamiPF04157. EAP30. 1 hit.
[Graphical view]
PIRSFiPIRSF017215. ESCRT2_Vps22. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSNF8_YEAST
AccessioniPrimary (citable) accession number: Q12483
Secondary accession number(s): D6W410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.