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Protein

Vacuolar-sorting protein SNF8

Gene

SNF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.1 Publication

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • protein targeting to vacuole Source: SGD
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33921-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein SNF8
Alternative name(s):
ESCRT-II complex subunit VPS22
Vacuolar protein-sorting-associated protein 22
Gene namesi
Name:SNF8
Synonyms:VPS22
Ordered Locus Names:YPL002C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL002C.
SGDiS000005923. SNF8.

Subcellular locationi

GO - Cellular componenti

  • ESCRT II complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Vacuolar-sorting protein SNF8PRO_0000215214Add
BLAST

Proteomic databases

MaxQBiQ12483.
PRIDEiQ12483.

Interactioni

Subunit structurei

Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS25P471424EBI-30277,EBI-25595
VPS36Q066965EBI-30277,EBI-36540

Protein-protein interaction databases

BioGridi36175. 67 interactions.
DIPiDIP-1745N.
IntActiQ12483. 5 interactions.
MINTiMINT-401148.

Structurei

Secondary structure

1
233
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi23 – 4927
Turni51 – 566
Helixi58 – 7114
Helixi75 – 784
Helixi83 – 853
Helixi88 – 10619
Turni107 – 1093
Beta strandi110 – 1123
Helixi116 – 1216
Turni122 – 1287
Helixi131 – 14111
Turni142 – 1443
Beta strandi149 – 1524
Beta strandi155 – 1584
Beta strandi161 – 1633
Helixi169 – 1746
Turni175 – 1795
Helixi184 – 1918
Helixi196 – 20712
Beta strandi210 – 2145
Beta strandi216 – 2205
Beta strandi222 – 2243
Helixi226 – 2305

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60A1-233[»]
1W7PX-ray3.60A1-233[»]
ProteinModelPortaliQ12483.
SMRiQ12483. Positions 20-232.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12483.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili23 – 4624Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the SNF8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000007843.
HOGENOMiHOG000170930.
InParanoidiQ12483.
KOiK12188.
OMAiWFPSLFP.
OrthoDBiEOG7CK3JD.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR016689. ESCRT-2_cplx_Snf8.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12806. PTHR12806. 1 hit.
PfamiPF04157. EAP30. 1 hit.
[Graphical view]
PIRSFiPIRSF017215. ESCRT2_Vps22. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

Q12483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQFGLAAFD ELKDGKYNDV NKTILEKQSV ELRDQLMVFQ ERLVEFAKKH
60 70 80 90 100
NSELQASPEF RSKFMHMCSS IGIDPLSLFD RDKHLFTVND FYYEVCLKVI
110 120 130 140 150
EICRQTKDMN GGVISFQELE KVHFRKLNVG LDDLEKSIDM LKSLECFEIF
160 170 180 190 200
QIRGKKFLRS VPNELTSDQT KILEICSILG YSSISLLKAN LGWEAVRSKS
210 220 230
ALDEMVANGL LWIDYQGGAE ALYWDPSWIT RQL
Length:233
Mass (Da):26,954
Last modified:November 1, 1996 - v1
Checksum:i212C74F086A0325E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10361 Genomic DNA. Translation: AAA86824.1.
U33335 Genomic DNA. Translation: AAB68103.1.
Z71255 Genomic DNA. Translation: CAA95039.1.
Z48483 Genomic DNA. Translation: CAA88384.1.
AY692759 Genomic DNA. Translation: AAT92778.1.
BK006949 Genomic DNA. Translation: DAA11426.1.
PIRiS52529.
RefSeqiNP_015323.1. NM_001183816.1.

Genome annotation databases

EnsemblFungiiYPL002C; YPL002C; YPL002C.
GeneIDi856105.
KEGGisce:YPL002C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10361 Genomic DNA. Translation: AAA86824.1.
U33335 Genomic DNA. Translation: AAB68103.1.
Z71255 Genomic DNA. Translation: CAA95039.1.
Z48483 Genomic DNA. Translation: CAA88384.1.
AY692759 Genomic DNA. Translation: AAT92778.1.
BK006949 Genomic DNA. Translation: DAA11426.1.
PIRiS52529.
RefSeqiNP_015323.1. NM_001183816.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60A1-233[»]
1W7PX-ray3.60A1-233[»]
ProteinModelPortaliQ12483.
SMRiQ12483. Positions 20-232.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36175. 67 interactions.
DIPiDIP-1745N.
IntActiQ12483. 5 interactions.
MINTiMINT-401148.

Proteomic databases

MaxQBiQ12483.
PRIDEiQ12483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL002C; YPL002C; YPL002C.
GeneIDi856105.
KEGGisce:YPL002C.

Organism-specific databases

EuPathDBiFungiDB:YPL002C.
SGDiS000005923. SNF8.

Phylogenomic databases

GeneTreeiENSGT00390000007843.
HOGENOMiHOG000170930.
InParanoidiQ12483.
KOiK12188.
OMAiWFPSLFP.
OrthoDBiEOG7CK3JD.

Enzyme and pathway databases

BioCyciYEAST:G3O-33921-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12483.
NextBioi981161.
PROiQ12483.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR016689. ESCRT-2_cplx_Snf8.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12806. PTHR12806. 1 hit.
PfamiPF04157. EAP30. 1 hit.
[Graphical view]
PIRSFiPIRSF017215. ESCRT2_Vps22. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of the SNF8 gene of Saccharomyces cerevisiae."
    Yeghiayan P., Tu J., Vallier L.G., Carlson M.
    Yeast 11:219-224(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body."
    Babst M., Katzmann D.J., Snyder W.B., Wendland B., Emr S.D.
    Dev. Cell 3:283-289(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes."
    Teo H., Perisic O., Gonzalez B., Williams R.L.
    Dev. Cell 7:559-569(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH VPS25 AND VPS36.
  8. "Structure of the ESCRT-II endosomal trafficking complex."
    Hierro A., Sun J., Rusnak A.S., Kim J., Prag G., Emr S.D., Hurley J.H.
    Nature 431:221-225(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH VPS25 AND VPS36.

Entry informationi

Entry nameiSNF8_YEAST
AccessioniPrimary (citable) accession number: Q12483
Secondary accession number(s): D6W410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.