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Protein

Vacuolar-sorting protein SNF8

Gene

SNF8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.1 Publication

Miscellaneous

Present with 1040 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • ATP export Source: SGD
  • carbon catabolite repression of transcription from RNA polymerase II promoter by glucose Source: SGD
  • protein targeting to vacuole Source: SGD
  • protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33921-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein SNF8
Alternative name(s):
ESCRT-II complex subunit VPS22
Vacuolar protein-sorting-associated protein 22
Gene namesi
Name:SNF8
Synonyms:VPS22
Ordered Locus Names:YPL002C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL002C
SGDiS000005923 SNF8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152141 – 233Vacuolar-sorting protein SNF8Add BLAST233

Proteomic databases

MaxQBiQ12483
PaxDbiQ12483
PRIDEiQ12483

Interactioni

Subunit structurei

Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36175124 interactors.
DIPiDIP-1745N
IntActiQ12483 16 interactors.
MINTiQ12483
STRINGi4932.YPL002C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60A1-233[»]
1W7PX-ray3.60A1-233[»]
ProteinModelPortaliQ12483
SMRiQ12483
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12483

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 46Sequence analysisAdd BLAST24

Sequence similaritiesi

Belongs to the SNF8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000007843
HOGENOMiHOG000170930
InParanoidiQ12483
KOiK12188
OMAiQYWLPAL
OrthoDBiEOG092C4ALW

Family and domain databases

Gene3Di1.10.10.102 hits
InterProiView protein in InterPro
IPR016689 ESCRT-2_cplx_Snf8
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR12806 PTHR12806, 1 hit
PIRSFiPIRSF017215 ESCRT2_Vps22, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits

Sequencei

Sequence statusi: Complete.

Q12483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQFGLAAFD ELKDGKYNDV NKTILEKQSV ELRDQLMVFQ ERLVEFAKKH
60 70 80 90 100
NSELQASPEF RSKFMHMCSS IGIDPLSLFD RDKHLFTVND FYYEVCLKVI
110 120 130 140 150
EICRQTKDMN GGVISFQELE KVHFRKLNVG LDDLEKSIDM LKSLECFEIF
160 170 180 190 200
QIRGKKFLRS VPNELTSDQT KILEICSILG YSSISLLKAN LGWEAVRSKS
210 220 230
ALDEMVANGL LWIDYQGGAE ALYWDPSWIT RQL
Length:233
Mass (Da):26,954
Last modified:November 1, 1996 - v1
Checksum:i212C74F086A0325E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10361 Genomic DNA Translation: AAA86824.1
U33335 Genomic DNA Translation: AAB68103.1
Z71255 Genomic DNA Translation: CAA95039.1
Z48483 Genomic DNA Translation: CAA88384.1
AY692759 Genomic DNA Translation: AAT92778.1
BK006949 Genomic DNA Translation: DAA11426.1
PIRiS52529
RefSeqiNP_015323.1, NM_001183816.1

Genome annotation databases

EnsemblFungiiYPL002C; YPL002C; YPL002C
GeneIDi856105
KEGGisce:YPL002C

Similar proteinsi

Entry informationi

Entry nameiSNF8_YEAST
AccessioniPrimary (citable) accession number: Q12483
Secondary accession number(s): D6W410
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome