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Protein

Protein AIR2

Gene

AIR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Both complexes polyadenylate RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. AIR2 also inhibits the methylation of NPL3 mediated by HMT1 through its interaction with HMT1.5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 7818CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri99 – 11618CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri162 – 17918CCHC-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • polynucleotide adenylyltransferase activity Source: InterPro
  • protein binding, bridging Source: SGD
  • RNA binding Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • ncRNA polyadenylation Source: SGD
  • nuclear mRNA surveillance of mRNA 3'-end processing Source: SGD
  • nuclear polyadenylation-dependent CUT catabolic process Source: SGD
  • nuclear polyadenylation-dependent rRNA catabolic process Source: SGD
  • nuclear polyadenylation-dependent snoRNA catabolic process Source: SGD
  • nuclear polyadenylation-dependent snRNA catabolic process Source: SGD
  • nuclear polyadenylation-dependent tRNA catabolic process Source: SGD
  • tRNA modification Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29563-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AIR2
Alternative name(s):
Arginine methyltransferase-interacting RING finger protein 2
Gene namesi
Name:AIR2
Ordered Locus Names:YDL175C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL175C.
SGDiS000002334. AIR2.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
  • TRAMP complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Protein AIR2PRO_0000227604Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei31 – 311PhosphoserineCombined sources
Modified residuei49 – 491PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12476.

PTM databases

iPTMnetiQ12476.

Interactioni

Subunit structurei

Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2. Interacts with HMT1 and NPL3. The interaction with NPL3 requires the presence of HMT1. Interacts directly with PAP2.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MTR4P470476EBI-31475,EBI-11592
PAP2P5363212EBI-31475,EBI-19517

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi31891. 63 interactions.
DIPiDIP-2543N.
IntActiQ12476. 14 interactions.
MINTiMINT-543316.

Structurei

Secondary structure

1
344
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 224Combined sources
Helixi26 – 283Combined sources
Helixi33 – 353Combined sources
Helixi36 – 405Combined sources
Beta strandi67 – 704Combined sources
Turni73 – 775Combined sources
Turni82 – 843Combined sources
Turni87 – 893Combined sources
Turni92 – 943Combined sources
Helixi95 – 973Combined sources
Beta strandi101 – 1055Combined sources
Beta strandi107 – 1104Combined sources
Turni111 – 1133Combined sources
Beta strandi124 – 1263Combined sources
Beta strandi129 – 1313Combined sources
Helixi133 – 1353Combined sources
Helixi137 – 1393Combined sources
Beta strandi145 – 1473Combined sources
Beta strandi165 – 1684Combined sources
Beta strandi170 – 1723Combined sources
Helixi174 – 1763Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LLINMR-A57-180[»]
3NYBX-ray2.70B118-198[»]
4U4CX-ray2.40B1-62[»]
ProteinModelPortaliQ12476.
SMRiQ12476. Positions 5-54, 57-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12476.

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR1 family.Curated
Contains 3 CCHC-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri61 – 7818CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri99 – 11618CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri162 – 17918CCHC-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00510000048226.
HOGENOMiHOG000000966.
InParanoidiQ12476.
KOiK12597.
OrthoDBiEOG092C2J4N.

Family and domain databases

Gene3Di4.10.60.10. 4 hits.
InterProiIPR016713. Air1/2_Saccharomycetales.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00098. zf-CCHC. 3 hits.
[Graphical view]
PIRSFiPIRSF018162. PolyA_pol_Air1/2. 1 hit.
SMARTiSM00343. ZnF_C2HC. 5 hits.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 3 hits.
PROSITEiPS50158. ZF_CCHC. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKNTAPFVV DTAPTTPPDK LVAPSIEEVN SNPNELRALR GQGRYFGVSD
60 70 80 90 100
DDKDAIKEAA PKCNNCSQRG HLKKDCPHII CSYCGATDDH YSRHCPKAIQ
110 120 130 140 150
CSKCDEVGHY RSQCPHKWKK VQCTLCKSKK HSKERCPSIW RAYILVDDNE
160 170 180 190 200
KAKPKVLPFH TIYCYNCGGK GHFGDDCKEK RSSRVPNEDG SAFTGSNLSV
210 220 230 240 250
ELKQEYYRHM NRNSDENEDY QFSESIYDED PLPRPSHKRH SQNDHSHSGR
260 270 280 290 300
NKRRASNFHP PPYQKSNVIQ PTIRGETLSL NNNISKNSRY QNTKVNVSSI
310 320 330 340
SENMYGSRYN PSTYVDNNSI SNSSNYRNYN SYQPYRSGTL GKRR
Length:344
Mass (Da):39,344
Last modified:November 1, 1996 - v1
Checksum:i5915C97FA39D6AFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74223 Genomic DNA. Translation: CAA98749.1.
Z67750 Genomic DNA. Translation: CAA91570.1.
BK006938 Genomic DNA. Translation: DAA11687.1.
PIRiS61037.
RefSeqiNP_010106.1. NM_001180235.1.

Genome annotation databases

EnsemblFungiiYDL175C; YDL175C; YDL175C.
GeneIDi851379.
KEGGisce:YDL175C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74223 Genomic DNA. Translation: CAA98749.1.
Z67750 Genomic DNA. Translation: CAA91570.1.
BK006938 Genomic DNA. Translation: DAA11687.1.
PIRiS61037.
RefSeqiNP_010106.1. NM_001180235.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LLINMR-A57-180[»]
3NYBX-ray2.70B118-198[»]
4U4CX-ray2.40B1-62[»]
ProteinModelPortaliQ12476.
SMRiQ12476. Positions 5-54, 57-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31891. 63 interactions.
DIPiDIP-2543N.
IntActiQ12476. 14 interactions.
MINTiMINT-543316.

PTM databases

iPTMnetiQ12476.

Proteomic databases

MaxQBiQ12476.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL175C; YDL175C; YDL175C.
GeneIDi851379.
KEGGisce:YDL175C.

Organism-specific databases

EuPathDBiFungiDB:YDL175C.
SGDiS000002334. AIR2.

Phylogenomic databases

GeneTreeiENSGT00510000048226.
HOGENOMiHOG000000966.
InParanoidiQ12476.
KOiK12597.
OrthoDBiEOG092C2J4N.

Enzyme and pathway databases

BioCyciYEAST:G3O-29563-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12476.
PROiQ12476.

Family and domain databases

Gene3Di4.10.60.10. 4 hits.
InterProiIPR016713. Air1/2_Saccharomycetales.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00098. zf-CCHC. 3 hits.
[Graphical view]
PIRSFiPIRSF018162. PolyA_pol_Air1/2. 1 hit.
SMARTiSM00343. ZnF_C2HC. 5 hits.
[Graphical view]
SUPFAMiSSF57756. SSF57756. 3 hits.
PROSITEiPS50158. ZF_CCHC. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIR2_YEAST
AccessioniPrimary (citable) accession number: Q12476
Secondary accession number(s): D6VRH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.