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Protein

Ferric reductase transmembrane component 6

Gene

FRE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metalloreductase responsible for reducing vacuolar iron and copper prior to transport into the cytosol. Catalyzes the reduction of Fe3+ to Fe2+ and Cu2+ to Cu+, respectively, which can then be transported by the respective vacuolar efflux systems to the cytosol.2 Publications

Catalytic activityi

2 Fe(II)-siderophore + NADP+ + H+ = 2 Fe(III)-siderophore + NADPH.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi323Iron (heme 1 axial ligand)By similarity1
Metal bindingi337Iron (heme 2 axial ligand)By similarity1
Metal bindingi393Iron (heme 1 axial ligand)By similarity1
Metal bindingi407Iron (heme 2 axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi493 – 499FADSequence analysis7
Nucleotide bindingi538 – 541NADPSequence analysis4
Nucleotide bindingi678 – 679NADPSequence analysis2

GO - Molecular functioni

GO - Biological processi

  • copper ion import Source: SGD
  • intracellular sequestering of iron ion Source: SGD
  • iron ion transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Ion transport, Iron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciYEAST:G3O-32150-MONOMER.

Protein family/group databases

TCDBi5.B.1.5.2. the phagocyte (gp91(phox)) nadph oxidase family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reductase transmembrane component 6 (EC:1.16.1.9)
Alternative name(s):
Ferric-chelate reductase 6
Gene namesi
Name:FRE6
Ordered Locus Names:YLL051C
ORF Names:L0593
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL051C.
SGDiS000003974. FRE6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 167VacuolarSequence analysisAdd BLAST150
Transmembranei168 – 188Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini189 – 244CytoplasmicSequence analysisAdd BLAST56
Transmembranei245 – 265Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini266 – 287VacuolarSequence analysisAdd BLAST22
Transmembranei288 – 308Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini309 – 328CytoplasmicSequence analysisAdd BLAST20
Transmembranei329 – 349Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini350 – 360VacuolarSequence analysisAdd BLAST11
Transmembranei361 – 381Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini382 – 387CytoplasmicSequence analysis6
Transmembranei388 – 408Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini409 – 416VacuolarSequence analysis8
Transmembranei417 – 437Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini438 – 712CytoplasmicSequence analysisAdd BLAST275

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi493 – 496HPFT → AAAA: Loss of function. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001014218 – 712Ferric reductase transmembrane component 6Add BLAST695

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Glycosylationi124N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ12473.
PRIDEiQ12473.

PTM databases

iPTMnetiQ12473.

Expressioni

Inductioni

By transcription factor AFT2 upon iron deprivation.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi31264. 10 interactors.
DIPiDIP-5019N.
MINTiMINT-565619.

Structurei

3D structure databases

ProteinModelPortaliQ12473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini287 – 411Ferric oxidoreductaseAdd BLAST125
Domaini412 – 546FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST135

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi553 – 556Poly-Leu4

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiQ12473.
KOiK00521.
OMAiSSARIHY.
OrthoDBiEOG092C04QA.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTLLFLTW LISLTKAFNI KLPHTEKKDH LESNAVLACA SYINTLKWSF
60 70 80 90 100
DSSVVPGFYS TICSYSPAFD TWSLCIFNSL TDQIIPMDNT SFEESLGNVR
110 120 130 140 150
KTCSFVDKKF SNISLEQYYS SLNNASSHAL EDYGSIESLS TSIRVDRETR
160 170 180 190 200
SRWIRAFHAH AYNLDISSVY GAYLTYYFVI VGIIAVFFHM SHYNGLNRAL
210 220 230 240 250
FASRFVNYIR GHFVLPTFLV DKHANHFKFL NVEVFTGLMP NSLEAWIIFG
260 270 280 290 300
YTLANIIFLS ISYIIDPYNL IFNSHLSQFT RLLADRSGIL AFTQFPLIII
310 320 330 340 350
FTARNSFLEF LTGVKFNSFI SFHKWIGRIM VLNATIHSLS YSLFAIINHA
360 370 380 390 400
FKISNKQLYW KFGIASITVL CVLLVLSLGI VRKRHYEFFL YTHIILALLF
410 420 430 440 450
FYCCWQHVKI FNGWKEWIVV SLLIWGLEKL FRIWNILQFR FPKATLINLN
460 470 480 490 500
TSNNPHDEMF KVIIPKYNRR WHSKPGQYCF IYFLHPLVFW QCHPFTIIDE
510 520 530 540 550
GEKCVLVIKP KSGLTRFIYN HILQSLNGKL QLRVAIEGPY GPSNLHLDKF
560 570 580 590 600
DHLLLLSGGT GLPGPLDHAI KLSRNPDKPK SIDLIMAIKN PSFLNGYKSE
610 620 630 640 650
ILELKNSRSH VNVQVYLTQK TAVTKAANAR DQLIHFDDIM TELTSFAHIG
660 670 680 690 700
NARPNFSNVI ENAIKSTPPG DSLAVVCCGP PVLVDDVRNT VSQKLLGYPE
710
RIIEYFEEYQ CW
Length:712
Mass (Da):81,989
Last modified:November 1, 1996 - v1
Checksum:i5224F12B51544BAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47973 Genomic DNA. Translation: CAA88006.1.
Z73156 Genomic DNA. Translation: CAA97503.1.
BK006945 Genomic DNA. Translation: DAA09273.1.
PIRiS50969.
RefSeqiNP_013049.1. NM_001181871.1.

Genome annotation databases

EnsemblFungiiYLL051C; YLL051C; YLL051C.
GeneIDi850675.
KEGGisce:YLL051C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47973 Genomic DNA. Translation: CAA88006.1.
Z73156 Genomic DNA. Translation: CAA97503.1.
BK006945 Genomic DNA. Translation: DAA09273.1.
PIRiS50969.
RefSeqiNP_013049.1. NM_001181871.1.

3D structure databases

ProteinModelPortaliQ12473.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31264. 10 interactors.
DIPiDIP-5019N.
MINTiMINT-565619.

Protein family/group databases

TCDBi5.B.1.5.2. the phagocyte (gp91(phox)) nadph oxidase family.

PTM databases

iPTMnetiQ12473.

Proteomic databases

MaxQBiQ12473.
PRIDEiQ12473.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLL051C; YLL051C; YLL051C.
GeneIDi850675.
KEGGisce:YLL051C.

Organism-specific databases

EuPathDBiFungiDB:YLL051C.
SGDiS000003974. FRE6.

Phylogenomic databases

GeneTreeiENSGT00390000007891.
HOGENOMiHOG000000805.
InParanoidiQ12473.
KOiK00521.
OMAiSSARIHY.
OrthoDBiEOG092C04QA.

Enzyme and pathway databases

BioCyciYEAST:G3O-32150-MONOMER.

Miscellaneous databases

PROiQ12473.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRE6_YEAST
AccessioniPrimary (citable) accession number: Q12473
Secondary accession number(s): D6VXV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.