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Protein

Serine/threonine-protein kinase SKM1

Gene

SKM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in cellular signaling or cytoskeletal functions. May play a role in morphogenetic control.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei406 – 4061ATPPROSITE-ProRule annotation
Active sitei507 – 5071Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi366 – 3749ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • cell separation after cytokinesis Source: SGD
  • negative regulation of gene expression Source: SGD
  • negative regulation of sterol import by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • protein phosphorylation Source: SGD
  • sterol import Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33510-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-SCE-399954. Sema3A PAK dependent Axon repulsion.
R-SCE-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase SKM1 (EC:2.7.11.1)
Alternative name(s):
Protein kinase 75490 D
Gene namesi
Name:SKM1
Ordered Locus Names:YOL113W
ORF Names:HRA655
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL113W.
SGDiS000005473. SKM1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655Serine/threonine-protein kinase SKM1PRO_0000086656Add
BLAST

Proteomic databases

MaxQBiQ12469.

PTM databases

iPTMnetiQ12469.

Interactioni

Protein-protein interaction databases

BioGridi34287. 106 interactions.
DIPiDIP-4217N.
IntActiQ12469. 6 interactions.
MINTiMINT-546388.

Structurei

3D structure databases

ProteinModelPortaliQ12469.
SMRiQ12469. Positions 15-55, 123-192, 342-642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 118116PHPROSITE-ProRule annotationAdd
BLAST
Domaini123 – 13614CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini360 – 639280Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000129768.
HOGENOMiHOG000234202.
InParanoidiQ12469.
KOiK08286.
OMAiEQATHAS.
OrthoDBiEOG708W7W.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGVKKEGWI SYKVDGLFSF LWQKRYLVLN DSYLAFYKSD KCNEEPVLSV
60 70 80 90 100
PLTSITNVSR IQLKQNCFEI LRATDQKENI SPINSYFYES NSKRSIFIST
110 120 130 140 150
RTERDLHGWL DAIFAKCPLL SGVSSPTNFT HKVHVGFDPK VGNFVGVPDS
160 170 180 190 200
WAKLLQTSEI TYDDWNRNSK AVIKALQFYE DYNGLDTMQF NDHLNTSLDL
210 220 230 240 250
KPLKSPTRYI INKRTNSIKR SVSRTLRKGK TDSILPVYQS ELKPFPRPSD
260 270 280 290 300
DDYKFTNIED NKVREEGRVH VSKESTADSQ TKQLGKKEQK VIQSHLRRHD
310 320 330 340 350
NNSTFRPHRL APSAPATKNH DSKTKWHKED LLELKNNDDS NEIIMKMKTV
360 370 380 390 400
AIDVNPRPYF QLVEKAGQGA SGAVYLSKRI KLPQENDPRF LKSHCHRVVG
410 420 430 440 450
ERVAIKQIRL SEQPKKQLIM NELLVMNDSR QENIVNFLEA YIIDDEELWV
460 470 480 490 500
IMEYMEGGCL TDILDAVARS NTGEHSSPLN ENQMAYIVKE TCQGLKFLHN
510 520 530 540 550
KKIIHRDIKS DNILLNSQGL VKITDFGFCV ELTEKRSKRA TMVGTPYWMA
560 570 580 590 600
PEIVNQKGYD EKVDVWSLGI MLIEMIEGEP PYLNEDPLKA LYLIANNGSP
610 620 630 640 650
KLRHPESVSK QTKQFLDACL QVNVESRASV RKLLTFEFLS MACSPEQLKV

SLKWH
Length:655
Mass (Da):75,331
Last modified:November 1, 1996 - v1
Checksum:i1F6CBC85FE10D385
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501S → R in AAT93238 (PubMed:17322287).Curated
Sequence conflicti303 – 3031S → A in CAA49163 (PubMed:9044278).Curated
Sequence conflicti306 – 3061R → K in CAA49163 (PubMed:9044278).Curated
Sequence conflicti320 – 3201H → Y (PubMed:9044278).Curated
Sequence conflicti322 – 3221S → R (PubMed:9044278).Curated
Sequence conflicti452 – 4521M → T in CAA49163 (PubMed:9044278).Curated
Sequence conflicti468 – 4681A → V in CAA49163 (PubMed:9044278).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69322 Genomic DNA. Translation: CAA49163.1.
Z48149 Genomic DNA. Translation: CAA88147.1.
Z74855 Genomic DNA. Translation: CAA99132.1.
AY693219 Genomic DNA. Translation: AAT93238.1.
BK006948 Genomic DNA. Translation: DAA10670.1.
PIRiS51884.
RefSeqiNP_014528.1. NM_001183367.1.

Genome annotation databases

EnsemblFungiiYOL113W; YOL113W; YOL113W.
GeneIDi854036.
KEGGisce:YOL113W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69322 Genomic DNA. Translation: CAA49163.1.
Z48149 Genomic DNA. Translation: CAA88147.1.
Z74855 Genomic DNA. Translation: CAA99132.1.
AY693219 Genomic DNA. Translation: AAT93238.1.
BK006948 Genomic DNA. Translation: DAA10670.1.
PIRiS51884.
RefSeqiNP_014528.1. NM_001183367.1.

3D structure databases

ProteinModelPortaliQ12469.
SMRiQ12469. Positions 15-55, 123-192, 342-642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34287. 106 interactions.
DIPiDIP-4217N.
IntActiQ12469. 6 interactions.
MINTiMINT-546388.

PTM databases

iPTMnetiQ12469.

Proteomic databases

MaxQBiQ12469.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL113W; YOL113W; YOL113W.
GeneIDi854036.
KEGGisce:YOL113W.

Organism-specific databases

EuPathDBiFungiDB:YOL113W.
SGDiS000005473. SKM1.

Phylogenomic databases

GeneTreeiENSGT00840000129768.
HOGENOMiHOG000234202.
InParanoidiQ12469.
KOiK08286.
OMAiEQATHAS.
OrthoDBiEOG708W7W.

Enzyme and pathway databases

BioCyciYEAST:G3O-33510-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-SCE-399954. Sema3A PAK dependent Axon repulsion.
R-SCE-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

PROiQ12469.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00786. PBD. 1 hit.
PF00169. PH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00233. PH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of SKM1, a Saccharomyces cerevisiae gene encoding a novel Ste20/PAK-like protein kinase."
    Martin H., Mendoza A., Rodriguez-Pachon J.M., Molina M., Nombela C.
    Mol. Microbiol. 23:431-444(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSKM1_YEAST
AccessioniPrimary (citable) accession number: Q12469
Secondary accession number(s): D6W1V4, E9P934, Q06940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.