ID   MRX4_YEAST              Reviewed;         430 AA.
AC   Q12467; D6W3K0;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 123.
DE   RecName: Full=MIOREX complex component 4 {ECO:0000305|PubMed:25683707};
DE   AltName: Full=Mitochondrial organization of gene expression protein 4 {ECO:0000303|PubMed:25683707};
DE   Flags: Precursor;
GN   Name=MRX4 {ECO:0000303|PubMed:25683707};
GN   OrderedLocusNames=YPL168W {ECO:0000312|SGD:S000006089};
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   PubMed=8948103;
RX   DOI=10.1002/(sici)1097-0061(199611)12:14<1483::aid-yea34>3.0.co;2-o;
RA   Purnelle B., Coster F., Goffeau A.;
RT   "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a
RT   small nuclear RNA, a new putative protein kinase and two new putative
RT   regulators.";
RL   Yeast 12:1483-1492(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169875;
RA   Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA   Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA   Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA   Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA   DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA   Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA   Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA   Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA   Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA   Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA   Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA   Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA   Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA   Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA   Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA   Vo D.H., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL   Nature 387:103-105(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   INDUCTION.
RX   PubMed=16278933; DOI=10.1002/yea.1302;
RA   de Lichtenberg U., Wernersson R., Jensen T.S., Nielsen H.B., Fausboell A.,
RA   Schmidt P., Hansen F.B., Knudsen S., Brunak S.;
RT   "New weakly expressed cell cycle-regulated genes in yeast.";
RL   Yeast 22:1191-1201(2005).
RN   [6]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=25683707; DOI=10.1016/j.celrep.2015.01.012;
RA   Kehrein K., Schilling R., Moller-Hergt B.V., Wurm C.A., Jakobs S.,
RA   Lamkemeyer T., Langer T., Ott M.;
RT   "Organization of mitochondrial gene expression in two distinct ribosome-
RT   containing assemblies.";
RL   Cell Rep. 10:843-853(2015).
CC   -!- FUNCTION: Component of MIOREX complexes, large expressome-like
CC       assemblies of ribosomes with factors involved in all the steps of post-
CC       transcriptional gene expression. {ECO:0000269|PubMed:25683707}.
CC   -!- SUBUNIT: Associates with the mitochondrial ribosome.
CC       {ECO:0000269|PubMed:25683707}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095}.
CC       Mitochondrion membrane {ECO:0000305}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- INDUCTION: During M phase of cell cycle. {ECO:0000269|PubMed:16278933}.
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DR   EMBL; X96770; CAA65553.1; -; Genomic_DNA.
DR   EMBL; Z73524; CAA97874.1; -; Genomic_DNA.
DR   EMBL; BK006949; DAA11266.1; -; Genomic_DNA.
DR   PIR; S65179; S65179.
DR   RefSeq; NP_015157.1; NM_001183982.1.
DR   AlphaFoldDB; Q12467; -.
DR   BioGRID; 36015; 104.
DR   IntAct; Q12467; 1.
DR   STRING; 4932.YPL168W; -.
DR   MaxQB; Q12467; -.
DR   PaxDb; 4932-YPL168W; -.
DR   PeptideAtlas; Q12467; -.
DR   EnsemblFungi; YPL168W_mRNA; YPL168W; YPL168W.
DR   GeneID; 855935; -.
DR   KEGG; sce:YPL168W; -.
DR   AGR; SGD:S000006089; -.
DR   SGD; S000006089; MRX4.
DR   VEuPathDB; FungiDB:YPL168W; -.
DR   eggNOG; ENOG502S553; Eukaryota.
DR   HOGENOM; CLU_674708_0_0_1; -.
DR   InParanoid; Q12467; -.
DR   OMA; CNDEEVY; -.
DR   OrthoDB; 2014617at2759; -.
DR   BioCyc; YEAST:G3O-34064-MONOMER; -.
DR   BioGRID-ORCS; 855935; 5 hits in 10 CRISPR screens.
DR   PRO; PR:Q12467; -.
DR   Proteomes; UP000002311; Chromosome XVI.
DR   RNAct; Q12467; Protein.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
PE   1: Evidence at protein level;
KW   Membrane; Mitochondrion; Reference proteome; Transit peptide;
KW   Transmembrane; Transmembrane helix.
FT   TRANSIT         1..27
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..430
FT                   /note="MIOREX complex component 4"
FT                   /id="PRO_0000238656"
FT   TRANSMEM        403..420
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   430 AA;  48827 MW;  235ACBBC212D0541 CRC64;
     MTVLYTSASL KKMKCLAFNM GMNCVRTVSH ARSGGAKFGG RNVFNIFDSK TPDSVRIKAF
     KNTIYQSAMG KGKTKFSAME INLITSLVRG YKGEGKKNAI NPLQTNVQIL NKLLLTHRLT
     DKDILEGMNL AAGPVNVAIP RDITPQEEKK KVELRNRKAE NMDLHPSRKM HIKELLHSLN
     LDMCNDEEVY QKISLYLQKN EESRTSVGAS QQNHVDIDIN SLKRYLQNIE KKARQKSAID
     KQKKNQARIY QWNTQSFSEI VPLSAGNILF KREPNRLWKR LQNGISVFLG SNGGGKKSKT
     TKKVLQGNNI LLHSLENNKD MTLSNNFDHS VFNINFTDLF GVINASGSPP DRVLNEINEI
     ELKGWKCVGN LYDNNKIVVF QSSNPLLEDT KIPQKSFTNS KRFLISLSAL LASFFAYYRY
     RLSQRQESKK
//