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Protein

Tricalbin-1

Gene

TCB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in membrane trafficking.2 Publications

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1008Calcium 1By similarity1
Metal bindingi1008Calcium 2By similarity1
Metal bindingi1014Calcium 1By similarity1
Metal bindingi1064Calcium 1By similarity1
Metal bindingi1064Calcium 2By similarity1
Metal bindingi1066Calcium 1By similarity1
Metal bindingi1066Calcium 2By similarity1
Metal bindingi1066Calcium 3By similarity1
Metal bindingi1069Calcium 3By similarity1
Metal bindingi1072Calcium 2By similarity1
Metal bindingi1072Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB-KW
  • lipid binding Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • endoplasmic reticulum membrane organization Source: SGD
  • regulation of phosphatidylinositol dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding, Lipid-binding, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33621-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tricalbin-1
Gene namesi
Name:TCB1
Ordered Locus Names:YOR086C
ORF Names:YOR3141c
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR086C.
SGDiS000005612. TCB1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 106CytoplasmicSequence analysisAdd BLAST106
Transmembranei107 – 127HelicalSequence analysisAdd BLAST21
Topological domaini128ExtracellularSequence analysis1
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 1186CytoplasmicSequence analysisAdd BLAST1037

GO - Cellular componenti

  • cortical endoplasmic reticulum Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002522711 – 1186Tricalbin-1Add BLAST1186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1000PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12466.
PRIDEiQ12466.

PTM databases

iPTMnetiQ12466.

Interactioni

Subunit structurei

Interacts with TCB2 via its C-terminal domain.1 Publication

Protein-protein interaction databases

BioGridi34484. 34 interactors.
DIPiDIP-6292N.
IntActiQ12466. 4 interactors.
MINTiMINT-705548.

Structurei

3D structure databases

ProteinModelPortaliQ12466.
SMRiQ12466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini373 – 471C2 1PROSITE-ProRule annotationAdd BLAST99
Domaini645 – 741C2 2PROSITE-ProRule annotationAdd BLAST97
Domaini979 – 1078C2 3PROSITE-ProRule annotationAdd BLAST100

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili795 – 822Sequence analysisAdd BLAST28

Domaini

The C-terminal C2 domain shows Ca2+-dependent phospholipid binding. It binds to phosphatidylserine, phosphatidylinositol and various phosphoinositides. The other C2 domains do not retain all 5 conserved Asp residues found in calcium-binding C2 domains.

Sequence similaritiesi

Belongs to the tricalbin family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000113103.
HOGENOMiHOG000248700.
InParanoidiQ12466.
OMAiPHIETVN.
OrthoDBiEOG092C0K2U.

Family and domain databases

Gene3Di2.60.40.150. 4 hits.
InterProiIPR000008. C2_dom.
IPR017147. Tricalbin.
[Graphical view]
PfamiPF00168. C2. 4 hits.
[Graphical view]
PIRSFiPIRSF037232. Tricalbin. 1 hit.
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 4 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 4 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEDTGVTA PKKPETAQVA NINGIDKLEP PKTKEETESS KSVSSEKAAH
60 70 80 90 100
ASDESFKRSI HEASYVGWKQ IGGWEDKDEL TLDDELMDMT RETFLDNIIP
110 120 130 140 150
DSLYGDWYHS VAIFFIGGVA SFALGHYKFS MGSAFFVIVI TSLLYRTSAK
160 170 180 190 200
KYRGSIRELV QKEFTVQKVE NDYESLEWLN AFLDKYWPIL EPSVSQLIVQ
210 220 230 240 250
QANEQMATNE AIPKFITQLW IDELTLGVKP PRVDLVKTFQ NTASDVVVMD
260 270 280 290 300
WGISFTPHDL CDMSAKQVRN YVNELAVVKA KIFGITIPVS VSDIAFKAHA
310 320 330 340 350
RVKFKLMTPF PHVETVNIQL LKVPDFDFVA TLFGRSIFNW EILAIPGLMT
360 370 380 390 400
LIQKMAKKYM GPILLPPFSL QLNIPQLLSG SNLSIGILEI TVKNAKGLKR
410 420 430 440 450
TSSILNESID PYLSFEFNDI SIAKTRTVRD TLNPVWDETL YVLLNSFTDP
460 470 480 490 500
LTISVYDKRA KLKDKVLGRI QYNLNTLHDK TTQRNLKAQF LRNSKPVGEL
510 520 530 540 550
TFDLRFFPTL EEKKLPDGSV EELPDLNTGI AKVVVEEGSR FAEEEQKVTA
560 570 580 590 600
YVEVYLNAKL VLTTGKATDT GTLKWNSDYE AVIADRRKTR YKFVVKDGKG
610 620 630 640 650
EEIGSTIQTL NDLIDRSQVN KNLIPLKNQK GDIKITTYWR PVRLEIGSNS
660 670 680 690 700
VAYTPPIGAI RVFIEKANDL RNLEKFGTID PYCKVLVNGL SKGRTDFKSQ
710 720 730 740 750
TLNPVWNQVI YVAVTSPNQR ITLQCMDVET VNKDRSLGEF NVNVQDLFKK
760 770 780 790 800
DENDKYEETI DEKAKVGRLV MPKKKPKGTI TYYTSFYPAL PVLTLEEIQD
810 820 830 840 850
LDKVNKKKKA LELRKSAIDE KKISKEDKAK FDQEWNEVKE LEDMYSNRQK
860 870 880 890 900
LDLPELLQYN QGVLAVTVLN GELPDSGLYV QAFFDDNGHP RFVSPRIPSR
910 920 930 940 950
IVKNGWSGDV IIKELDKSIT TFRVAKNKNY NRVEKCVCEV ELPTQELVKN
960 970 980 990 1000
CYYKPSILHL SGEGSAKLML QISWFPIDTK QLPANDLITN SGDLTIMSRS
1010 1020 1030 1040 1050
AENLIASDLN GYSDPYLKYY INNEEDCAYK TKVVKKTLNP KWNDEGTIQI
1060 1070 1080 1090 1100
NNRLNDVLRI KVMDWDSTSA DDTIGTAEIP LNKVKVEGTT ELDVPVEGLE
1110 1120 1130 1140 1150
NAGQDGGMLH LAFSFKPRYT ISVSKREKKV GDIASKGLGT GLKAGTTVIG
1160 1170 1180
GGVGAIGKIK KGVFGGLGSL TNHKKNHEMG EEETKF
Length:1,186
Mass (Da):133,576
Last modified:November 1, 1996 - v1
Checksum:i1CD852549A6D82B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64008.1.
Z74994 Genomic DNA. Translation: CAA99281.1.
BK006948 Genomic DNA. Translation: DAA10864.1.
PIRiS61647.
RefSeqiNP_014729.1. NM_001183505.1.

Genome annotation databases

EnsemblFungiiYOR086C; YOR086C; YOR086C.
GeneIDi854253.
KEGGisce:YOR086C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64008.1.
Z74994 Genomic DNA. Translation: CAA99281.1.
BK006948 Genomic DNA. Translation: DAA10864.1.
PIRiS61647.
RefSeqiNP_014729.1. NM_001183505.1.

3D structure databases

ProteinModelPortaliQ12466.
SMRiQ12466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34484. 34 interactors.
DIPiDIP-6292N.
IntActiQ12466. 4 interactors.
MINTiMINT-705548.

PTM databases

iPTMnetiQ12466.

Proteomic databases

MaxQBiQ12466.
PRIDEiQ12466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR086C; YOR086C; YOR086C.
GeneIDi854253.
KEGGisce:YOR086C.

Organism-specific databases

EuPathDBiFungiDB:YOR086C.
SGDiS000005612. TCB1.

Phylogenomic databases

GeneTreeiENSGT00730000113103.
HOGENOMiHOG000248700.
InParanoidiQ12466.
OMAiPHIETVN.
OrthoDBiEOG092C0K2U.

Enzyme and pathway databases

BioCyciYEAST:G3O-33621-MONOMER.

Miscellaneous databases

PROiQ12466.

Family and domain databases

Gene3Di2.60.40.150. 4 hits.
InterProiIPR000008. C2_dom.
IPR017147. Tricalbin.
[Graphical view]
PfamiPF00168. C2. 4 hits.
[Graphical view]
PIRSFiPIRSF037232. Tricalbin. 1 hit.
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 4 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 4 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCB1_YEAST
AccessioniPrimary (citable) accession number: Q12466
Secondary accession number(s): D6W2E8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.