Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RuvB-like protein 2

Gene

RVB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing.9 Publications

Miscellaneous

Present with 3030 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 82ATPBy similarity8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: SGD
  • ATP-dependent 5'-3' DNA helicase activity Source: SGD

GO - Biological processi

  • box C/D snoRNP assembly Source: SGD
  • chromatin remodeling Source: SGD
  • DNA repair Source: UniProtKB-KW
  • histone acetylation Source: GO_Central
  • histone exchange Source: SGD
  • regulation of transcription from RNA polymerase II promoter Source: SGD
  • rRNA processing Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Chromatin regulator, Helicase, Hydrolase
Biological processDNA damage, DNA repair, rRNA processing, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34122-MONOMER.
ReactomeiR-SCE-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
RuvB-like protein 2 (EC:3.6.4.12)
Short name:
RUVBL2
Alternative name(s):
TIP49-homology protein 2
TIP49b homolog
Gene namesi
Name:RVB2
Synonyms:TIH2, TIP49B
Ordered Locus Names:YPL235W
ORF Names:P1060
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL235W.
SGDiS000006156. RVB2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi75G → A: Lethal. 1 Publication1
Mutagenesisi80G → A: Growth defect at 37 degrees Celsius. 1 Publication1
Mutagenesisi81K → A: Defect in snoRNA accumulation. Growth defect at 37 degrees Celsius. 3 Publications1
Mutagenesisi81K → E: Lethal. 3 Publications1
Mutagenesisi81K → R: Growth defect at 37 degrees Celsius. 3 Publications1
Mutagenesisi296D → N: Lethal. 1 Publication1
Mutagenesisi297E → G: Lethal. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001656751 – 471RuvB-like protein 2Add BLAST471

Proteomic databases

MaxQBiQ12464.
PRIDEiQ12464.

PTM databases

iPTMnetiQ12464.

Interactioni

Subunit structurei

Probably forms a homohexamer. Interacts with RVB1 and may form heterododecamers with RVB1. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Belongs also to the R2TP complex composed of at least RVB1, RVB2, TAH1 and PIH1. Interacts with SPT15/TBP.9 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35927. 487 interactors.
DIPiDIP-5207N.
IntActiQ12464. 180 interactors.
MINTiMINT-523843.
STRINGi4932.YPL235W.

Structurei

3D structure databases

ProteinModelPortaliQ12464.
SMRiQ12464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.Curated

Phylogenomic databases

GeneTreeiENSGT00890000139527.
HOGENOMiHOG000190885.
InParanoidiQ12464.
KOiK11338.
OMAiVPFTMIA.
OrthoDBiEOG092C2Z17.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR027238. RuvB-like.
IPR010339. TIP49_C.
PANTHERiPTHR11093. PTHR11093. 1 hit.
PfamiView protein in Pfam
PF06068. TIP49. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIQTSDPNE TSDLKSLSLI AAHSHITGLG LDENLQPRPT SEGMVGQLQA
60 70 80 90 100
RRAAGVILKM VQNGTIAGRA VLVAGPPSTG KTALAMGVSQ SLGKDVPFTA
110 120 130 140 150
IAGSEIFSLE LSKTEALTQA FRKSIGIKIK EETELIEGEV VEIQIDRSIT
160 170 180 190 200
GGHKQGKLTI KTTDMETIYE LGNKMIDGLT KEKVLAGDVI SIDKASGKIT
210 220 230 240 250
KLGRSFARSR DYDAMGADTR FVQCPEGELQ KRKTVVHTVS LHEIDVINSR
260 270 280 290 300
TQGFLALFTG DTGEIRSEVR DQINTKVAEW KEEGKAEIVP GVLFIDEVHM
310 320 330 340 350
LDIECFSFIN RALEDEFAPI VMMATNRGVS KTRGTNYKSP HGLPLDLLDR
360 370 380 390 400
SIIITTKSYN EQEIKTILSI RAQEEEVELS SDALDLLTKT GVETSLRYSS
410 420 430 440 450
NLISVAQQIA MKRKNNTVEV EDVKRAYLLF LDSARSVKYV QENESQYIDD
460 470
QGNVQISIAK SADPDAMDTT E
Length:471
Mass (Da):51,612
Last modified:November 1, 1996 - v1
Checksum:i0D8EFBA7EC711AB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z67751 Genomic DNA. Translation: CAA91609.1.
X94561 Genomic DNA. Translation: CAA64252.1.
Z73591 Genomic DNA. Translation: CAA97952.1.
BK006949 Genomic DNA. Translation: DAA11201.1.
PIRiS61029.
RefSeqiNP_015089.1. NM_001184049.1.

Genome annotation databases

EnsemblFungiiYPL235W; YPL235W; YPL235W.
GeneIDi855841.
KEGGisce:YPL235W.

Similar proteinsi

Entry informationi

Entry nameiRUVB2_YEAST
AccessioniPrimary (citable) accession number: Q12464
Secondary accession number(s): D6W3D5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names