RuvB-like protein 2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12464 (RUVB2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
RuvB-like protein 2
Short name=RUVBL2
EC=3.6.4.12

Alternative name(s):
TIP49-homology protein 2
TIP49b homolog

Gene names

Name:	RVB2
Synonyms:	TIH2, TIP49B
Ordered Locus Names:	YPL235W
ORF Names:	P1060

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA helicase which participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes. The SWR1 complex mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. The INO80 complex remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to 'Ser-129' phosphorylated H2A histones as a response to DNA damage. During transcription may recruit SPT15/TBP to the TATA-boxes of involved genes. Required for box C/D and box H/ACA snoRNA accumulation and involved in pre-rRNA processing. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 Ref.12
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Probably forms a homohexamer. Interacts with RVB1 and may form heterododecamers with RVB1. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Belongs also to the R2TP complex composed of at least RVB1, RVB2, TAH1 and PIH1. Interacts with SPT15/TBP. Ref.3 Ref.5 Ref.7
Subcellular location	Nucleus › nucleoplasm Ref.3 Ref.6.
Miscellaneous	Present with 3030 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation rRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Chromatin regulator Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW chromatin remodeling Inferred from direct assay Ref.12. Source: SGD rRNA processing Inferred from mutant phenotype Ref.6. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.5. Source: SGD snoRNA metabolic process Inferred from mutant phenotype Ref.6. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ASTRA complex Inferred from direct assay. Source: SGD Ino80 complex Inferred from physical interaction Ref.4. Source: SGD Swr1 complex Inferred from direct assay Ref.12 Ref.8. Source: SGD nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from mutant phenotype Ref.5. Source: SGD DNA helicase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.7. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
RVB1	Q03940	8	EBI-31814,EBI-30712
SPT15	P13393	3	EBI-31814,EBI-19129
SWC4	P53201	3	EBI-31814,EBI-23061

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 471

471

RuvB-like protein 2

PRO_0000165675

Regions

Nucleotide binding

75 – 82

ATP By similarity

Amino acid modifications

Modified residue

212

Phosphotyrosine Ref.14

Experimental info

Mutagenesis

G → A: Lethal. Ref.6

Mutagenesis

G → A: Growth defect at 37 degrees Celsius. Ref.6

Mutagenesis

K → A: Defect in snoRNA accumulation. Growth defect at 37 degrees Celsius. Ref.3 Ref.5 Ref.6

Mutagenesis

K → E: Lethal. Ref.3 Ref.5 Ref.6

Mutagenesis

K → R: Growth defect at 37 degrees Celsius. Ref.3 Ref.5 Ref.6

Mutagenesis

296

D → N: Lethal. Ref.6

Mutagenesis

297

E → G: Lethal. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

Q12464 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 0D8EFBA7EC711AB8
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FASTA

471

51,612

        10         20         30         40         50         60 
MSIQTSDPNE TSDLKSLSLI AAHSHITGLG LDENLQPRPT SEGMVGQLQA RRAAGVILKM 

        70         80         90        100        110        120 
VQNGTIAGRA VLVAGPPSTG KTALAMGVSQ SLGKDVPFTA IAGSEIFSLE LSKTEALTQA 

       130        140        150        160        170        180 
FRKSIGIKIK EETELIEGEV VEIQIDRSIT GGHKQGKLTI KTTDMETIYE LGNKMIDGLT 

       190        200        210        220        230        240 
KEKVLAGDVI SIDKASGKIT KLGRSFARSR DYDAMGADTR FVQCPEGELQ KRKTVVHTVS 

       250        260        270        280        290        300 
LHEIDVINSR TQGFLALFTG DTGEIRSEVR DQINTKVAEW KEEGKAEIVP GVLFIDEVHM 

       310        320        330        340        350        360 
LDIECFSFIN RALEDEFAPI VMMATNRGVS KTRGTNYKSP HGLPLDLLDR SIIITTKSYN 

       370        380        390        400        410        420 
EQEIKTILSI RAQEEEVELS SDALDLLTKT GVETSLRYSS NLISVAQQIA MKRKNNTVEV 

       430        440        450        460        470 
EDVKRAYLLF LDSARSVKYV QENESQYIDD QGNVQISIAK SADPDAMDTT E

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The Saccharomyces cerevisiae RuvB-like protein, Tih2p, is required for cell cycle progression and RNA polymerase II-directed transcription." Lim C.R., Kimata Y., Ohdate H., Kokubo T., Kikuchi N., Horigome T., Kohno K. J. Biol. Chem. 275:22409-22417(2000) [PubMed: 10787406] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-81, INTERACTION WITH RVB1.
[4]	"A chromatin remodelling complex involved in transcription and DNA processing." Shen X., Mizuguchi G., Hamiche A., Wu C. Nature 406:541-544(2000) [PubMed: 10952318] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION OF THE INO80 COMPLEX, MASS SPECTROMETRY.
[5]	"Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes." Jonsson Z.O., Dhar S.K., Narlikar G.J., Auty R., Wagle N., Pellman D., Pratt R.E., Kingston R., Dutta A. J. Biol. Chem. 276:16279-16288(2001) [PubMed: 11278922] [Abstract] Cited for: FUNCTION, INTERACTION WITH RVB1, IDENTIFICATION IN A COMPLEX WITH RBV1; ACT1 AND ARP4, MASS SPECTROMETRY, MUTAGENESIS OF LYS-81 AND GLU-297.
[6]	"A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins." King T.H., Decatur W.A., Bertrand E., Maxwell E.S., Fournier M.J. Mol. Cell. Biol. 21:7731-7746(2001) [PubMed: 11604509] [Abstract] Cited for: FUNCTION IN SNORNA SYNTHESIS, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-75; GLY-80; LYS-81 AND ASP-296.
[7]	"Impairment of the DNA binding activity of the TATA-binding protein renders the transcriptional function of Rvb2p/Tih2p, the yeast RuvB-like protein, essential for cell growth." Ohdate H., Lim C.R., Kokubo T., Matsubara K., Kimata Y., Kohno K. J. Biol. Chem. 278:14647-14656(2003) [PubMed: 12576485] [Abstract] Cited for: FUNCTION, INTERACTION WITH SPT15.
[8]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed: 14690608] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex." Jonsson Z.O., Jha S., Wohlschlegel J.A., Dutta A. Mol. Cell 16:465-477(2004) [PubMed: 15525518] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE INO80 COMPLEX, MASS SPECTROMETRY.
[11]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed: 15045029] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[12]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed: 14645854] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[13]	"Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone." Zhao R., Davey M.G., Hsu Y.-C., Kaplanek P., Tong A., Parsons A.B., Krogan N.J., Cagney G., Mai D., Greenblatt J.F., Boone C., Emili A., Houry W.A. Cell 120:715-727(2005) [PubMed: 15766533] [Abstract] Cited for: IDENTIFICATION IN THE R2PT COMPLEX, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-212, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z67751 Genomic DNA. Translation: CAA91609.1. X94561 Genomic DNA. Translation: CAA64252.1. Z73591 Genomic DNA. Translation: CAA97952.1. BK006949 Genomic DNA. Translation: DAA11201.1.
PIR	S61029.
RefSeq	NP_015089.1. NM_001184049.1.
3D structure databases
ProteinModelPortal	Q12464.
SMR	Q12464. Positions 20-452.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5207N.
IntAct	Q12464. 161 interactions.
MINT	MINT-523843.
STRING	Q12464.
Proteomic databases
PeptideAtlas	Q12464.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL235W; YPL235W; YPL235W.
GeneID	855841.
KEGG	sce:YPL235W.
NMPDR	fig\|4932.3.peg.6216.
Organism-specific databases
CYGD	YPL235w.
SGD	S000006156. RVB2.
Phylogenomic databases
eggNOG	fuNOG04234.
GeneTree	EFGT00050000004573.
HOGENOM	HBG749829.
OMA	TQAFRKS.
OrthoDB	EOG4W3WWK.
Gene expression databases
ArrayExpress	Q12464.
Genevestigator	Q12464.
GermOnline	YPL235W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR016027. NA-bd_OB-fold-like. IPR010339. TIP49_C. [Graphical view]
KO	K11338.
Pfam	PF06068. TIP49. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...
Other
NextBio	980419.

Entry information

Entry name

RUVB2_YEAST

Accession

Primary (citable) accession number: Q12464
Secondary accession number(s): D6W3D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents