Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine(10)-N2)-methyltransferase

Gene

TRM11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.1 Publication

Miscellaneous

Present with 2020 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(10) in tRNA = S-adenosyl-L-homocysteine + N2-methylguanine(10) in tRNA.1 Publication

GO - Molecular functioni

  • RNA binding Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD
  • tRNA (guanine-N2-)-methyltransferase activity Source: SGD
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • tRNA methylation Source: SGD
  • tRNA modification Source: Reactome

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33520-MONOMER
YEAST:G3O-33520-MONOMER
BRENDAi2.1.1.214 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(10)-N2)-methyltransferase (EC:2.1.1.214)
Alternative name(s):
tRNA [Gm10] methyltransferase
tRNA guanosine-2'-O-methyltransferase TRM11
tRNA methylase 11
Gene namesi
Name:TRM11
Ordered Locus Names:YOL124C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL124C
SGDiS000005484 TRM11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi215D → A: Abolishes activity. 1 Publication1
Mutagenesisi291D → A: Abolishes activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002709221 – 433tRNA (guanine(10)-N2)-methyltransferaseAdd BLAST433

Proteomic databases

MaxQBiQ12463
PaxDbiQ12463
PRIDEiQ12463

Interactioni

Subunit structurei

Interacts with TRM112.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TRM112P537386EBI-30471,EBI-28520

Protein-protein interaction databases

BioGridi34251, 175 interactors
DIPiDIP-6751N
IntActiQ12463, 5 interactors
MINTiQ12463
STRINGi4932.YOL124C

Structurei

3D structure databases

ProteinModelPortaliQ12463
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000018131
HOGENOMiHOG000191866
InParanoidiQ12463
KOiK15430
OMAiEKCPESH
OrthoDBiEOG092C3GCF

Family and domain databases

InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR000241 RNA_methylase_dom
IPR029063 SAM-dependent_MTases
IPR016691 tRNA_mtfrase_TRM11
PfamiView protein in Pfam
PF01170 UPF0020, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit
PS51627 SAM_MT_TRM11, 1 hit

Sequencei

Sequence statusi: Complete.

Q12463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKYLLYMVQ VHLNFRRAEL ESLADLYNLS IDFSQYDANS PFFIVELEND
60 70 80 90 100
QQAKDWIKRS ILTRGIYEYW GQGTTLDELH KDIQRQSNFE QDLQLKFKHS
110 120 130 140 150
TFKFEFECYK GNSKAKRVEQ IETFRYLGFE GKIDMKHPQE VFTVIEEYTP
160 170 180 190 200
ISENVGGKTP TRIYFGRQVQ MSNRSAMEKY DLKKRPYKGT TSFEAELSLV
210 220 230 240 250
SANIAQVKPG TIMYDPFAGT GSFLVAGGHF GSLVIGSDID GRMIRGKGAQ
260 270 280 290 300
VNISANFKKY GESSQFLDVL TMDFTNNALR NNLVIDTILC DPPYGIRESI
310 320 330 340 350
KVLGAKDPER FLGKEDMEID GEKAYLRRDY IPTKKPYALD SLLDDLLQYS
360 370 380 390 400
SERLPIGGRL AFWMPTANDA NIETIVPMHE NLELKYNCVQ EFNKWSRRLL
410 420 430
VYINRGSTFN GSSNHGIKRS KDNFRERYFN NFN
Length:433
Mass (Da):50,073
Last modified:November 1, 1996 - v1
Checksum:i61B291029D55263D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41293 Genomic DNA Translation: AAC49469.1
Z74866 Genomic DNA Translation: CAA99143.1
BK006948 Genomic DNA Translation: DAA10660.1
PIRiS63447
RefSeqiNP_014517.1, NM_001183378.1

Genome annotation databases

EnsemblFungiiYOL124C; YOL124C; YOL124C
GeneIDi853996
KEGGisce:YOL124C

Similar proteinsi

Entry informationi

Entry nameiTRM11_YEAST
AccessioniPrimary (citable) accession number: Q12463
Secondary accession number(s): D6W1U4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health