Q12463 (TRM11_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(10)-N2)-methyltransferase
EC=2.1.1.214

Alternative name(s):
tRNA [Gm10] methyltransferase
tRNA guanosine-2'-O-methyltransferase TRM11
tRNA methylase 11

Gene names

Name:	TRM11
Ordered Locus Names:	YOL124C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs. Ref.6
Catalytic activity	S-adenosyl-L-methionine + guanine(10) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(10) in tRNA.
Subunit structure	Interacts with TRM112. Ref.6
Subcellular location	Cytoplasm Ref.4 Ref.6.
Miscellaneous	Present with 2020 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the methyltransferase superfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	tRNA (m2G10) methyltransferase complex Inferred from physical interaction Ref.6. Source: SGD
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct tRNA (guanine-N2-)-methyltransferase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRM112	P53738	4	EBI-30471,EBI-28520

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	tRNA (guanine(10)-N2)-methyltransferase		PRO_0000270922
Experimental info
	Mutagenesis	215	1	D → A: Abolishes activity. Ref.6
	Mutagenesis	291	1	D → A: Abolishes activity. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q12463 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 61B291029D55263D	FASTA	433	50,073
	10 20 30 40 50 60 MKKYLLYMVQ VHLNFRRAEL ESLADLYNLS IDFSQYDANS PFFIVELEND QQAKDWIKRS 70 80 90 100 110 120 ILTRGIYEYW GQGTTLDELH KDIQRQSNFE QDLQLKFKHS TFKFEFECYK GNSKAKRVEQ 130 140 150 160 170 180 IETFRYLGFE GKIDMKHPQE VFTVIEEYTP ISENVGGKTP TRIYFGRQVQ MSNRSAMEKY 190 200 210 220 230 240 DLKKRPYKGT TSFEAELSLV SANIAQVKPG TIMYDPFAGT GSFLVAGGHF GSLVIGSDID 250 260 270 280 290 300 GRMIRGKGAQ VNISANFKKY GESSQFLDVL TMDFTNNALR NNLVIDTILC DPPYGIRESI 310 320 330 340 350 360 KVLGAKDPER FLGKEDMEID GEKAYLRRDY IPTKKPYALD SLLDDLLQYS SERLPIGGRL 370 380 390 400 410 420 AFWMPTANDA NIETIVPMHE NLELKYNCVQ EFNKWSRRLL VYINRGSTFN GSSNHGIKRS 430 KDNFRERYFN NFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames." Casamayor A., Khalid H., Balcells L., Aldea M., Casas C., Herrero E., Arino J. Yeast 12:1013-1020(1996) [PubMed: 8896265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA." Purushothaman S.K., Bujnicki J.M., Grosjean H., Lapeyre B. Mol. Cell. Biol. 25:4359-4370(2005) [PubMed: 15899842] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH TRM112, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-215 AND ASP-291.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41293 Genomic DNA. Translation: AAC49469.1. Z74866 Genomic DNA. Translation: CAA99143.1. BK006948 Genomic DNA. Translation: DAA10660.1.
PIR	S63447.
RefSeq	NP_014517.1. NM_001183378.1.
3D structure databases
ProteinModelPortal	Q12463.
SMR	Q12463. Positions 186-299.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6751N.
IntAct	Q12463. 1 interaction.
MINT	MINT-641577.
STRING	Q12463.
Proteomic databases
PeptideAtlas	Q12463.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL124C; YOL124C; YOL124C.
GeneID	853996.
KEGG	sce:YOL124C.
NMPDR	fig|4932.3.peg.5603.
Organism-specific databases
CYGD	YOL124c.
SGD	S000005484. TRM11.
Phylogenomic databases
eggNOG	fuNOG07732.
GeneTree	EFGT00050000002394.
HOGENOM	HBG738188.
OMA	FWILNIP.
OrthoDB	EOG444PTQ.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16616.
Gene expression databases
ArrayExpress	Q12463.
Genevestigator	Q12463.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR000241. RNA_methylase. IPR016691. tRNA_mtfrase_TRM11. [Graphical view]
KO	K15430.
Pfam	PF01170. UPF0020. 1 hit. [Graphical view]
PIRSF	PIRSF017259. tRNA_mtfrase_TRM11. 1 hit.
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975488.

Entry information

Entry name

TRM11_YEAST

Accession

Primary (citable) accession number: Q12463
Secondary accession number(s): D6W1U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program