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Q12461

- PTPA2_YEAST

UniProt

Q12461 - PTPA2_YEAST

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Protein
Serine/threonine-protein phosphatase 2A activator 2
Gene
RRD2, NOH1, YPA2, YPL152W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for TAP42-associated PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg2+ by dissociating the inactive form from the complex. Acts also inhibitory at high concentrations. Involved in the regulation of cell cycle progression, mitotic spindle formation and bud morphogenesis.6 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  1. peptidyl-prolyl cis-trans isomerase activity Source: SGD
  2. phosphatase activator activity Source: InterPro
  3. protein phosphatase type 2A regulator activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. mitotic spindle organization in nucleus Source: SGD
  2. protein folding Source: UniProtKB-KW
  3. protein peptidyl-prolyl isomerization Source: GOC
  4. regulation of catalytic activity Source: GOC
  5. response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Enzyme and pathway databases

BioCyciYEAST:G3O-34049-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A activator 2 (EC:5.2.1.8)
Alternative name(s):
Peptidyl-prolyl cis-trans isomerase PTPA-2
Short name:
PPIase PTPA-2
Short name:
Rotamase PTPA-2
Phosphotyrosyl phosphatase activator 2
Gene namesi
Name:RRD2
Synonyms:NOH1, YPA2
Ordered Locus Names:YPL152W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL152w.
SGDiS000006073. RRD2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. protein phosphatase type 2A complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Serine/threonine-protein phosphatase 2A activator 2
PRO_0000226120Add
BLAST

Proteomic databases

MaxQBiQ12461.
PaxDbiQ12461.
PeptideAtlasiQ12461.

Expressioni

Gene expression databases

GenevestigatoriQ12461.

Interactioni

Subunit structurei

Interacts with the phosphatase PP2A catalytic subunits PPH21 and PPH22. Forms a ternary complex with PPH21-TAP42.3 Publications

Protein-protein interaction databases

BioGridi36031. 63 interactions.
DIPiDIP-2558N.
IntActiQ12461. 3 interactions.
MINTiMINT-556521.
STRINGi4932.YPL152W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni10 – 134
Helixi14 – 174
Helixi20 – 3415
Turni35 – 384
Helixi50 – 6617
Helixi84 – 10320
Helixi109 – 12012
Turni126 – 1294
Helixi133 – 14816
Helixi154 – 16310
Helixi165 – 18016
Beta strandi192 – 1965
Helixi199 – 2079
Turni208 – 2103
Helixi216 – 2205
Helixi222 – 2287
Turni229 – 2313
Helixi233 – 24412
Turni245 – 2473
Helixi250 – 2534
Helixi255 – 2606
Helixi266 – 28015
Turni281 – 2833
Helixi285 – 2884
Beta strandi295 – 2973

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IXNX-ray2.80A/B1-304[»]
ProteinModelPortaliQ12461.
SMRiQ12461. Positions 3-299.

Miscellaneous databases

EvolutionaryTraceiQ12461.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG5057.
HOGENOMiHOG000205736.
KOiK17605.
OMAiFRTFYDK.
OrthoDBiEOG76HQD0.

Family and domain databases

InterProiIPR004327. Phstyr_phstse_ac.
[Graphical view]
PANTHERiPTHR10012. PTHR10012. 1 hit.
PfamiPF03095. PTPA. 1 hit.
[Graphical view]
PIRSFiPIRSF016325. Phstyr_phstse_ac. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12461-1 [UniParc]FASTAAdd to Basket

« Hide

MLPEKRLLTP DDMKLWEESP TRAHFTKFII DLAESVKGHE NSQYKEPISE    50
SINSMMNLLS QIKDITQKHP VIKDADSSRF GKVEFRDFYD EVSRNSRKIL 100
RSEFPSLTDE QLEQLSIYLD ESWGNKRRID YGSGHELNFM CLLYGLYSYG 150
IFNLSNDSTN LVLKVFIEYL KIMRILETKY WLEPAGSHGV WGLDDYHFLP 200
FLFGAFQLTT HKHLKPISIH NNELVEMFAH RYLYFGCIAF INKVKSSASL 250
RWHSPMLDDI SGVKTWSKVA EGMIKMYKAE VLSKLPIMQH FYFSEFLPCP 300
DGVSPPRGHI HDGTDKDDEC NFEGHVHTTW GDCCGIKLPS AIAATEMNKK 350
HHKPIPFD 358
Length:358
Mass (Da):41,452
Last modified:November 1, 1996 - v1
Checksum:iE0BE8A7EDD54E306
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96770 Genomic DNA. Translation: CAA65569.1.
Z73508 Genomic DNA. Translation: CAA97857.1.
BK006949 Genomic DNA. Translation: DAA11283.1.
PIRiS65163.
RefSeqiNP_015173.1. NM_001183966.1.

Genome annotation databases

EnsemblFungiiYPL152W; YPL152W; YPL152W.
GeneIDi855951.
KEGGisce:YPL152W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X96770 Genomic DNA. Translation: CAA65569.1 .
Z73508 Genomic DNA. Translation: CAA97857.1 .
BK006949 Genomic DNA. Translation: DAA11283.1 .
PIRi S65163.
RefSeqi NP_015173.1. NM_001183966.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IXN X-ray 2.80 A/B 1-304 [» ]
ProteinModelPortali Q12461.
SMRi Q12461. Positions 3-299.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36031. 63 interactions.
DIPi DIP-2558N.
IntActi Q12461. 3 interactions.
MINTi MINT-556521.
STRINGi 4932.YPL152W.

Proteomic databases

MaxQBi Q12461.
PaxDbi Q12461.
PeptideAtlasi Q12461.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL152W ; YPL152W ; YPL152W .
GeneIDi 855951.
KEGGi sce:YPL152W.

Organism-specific databases

CYGDi YPL152w.
SGDi S000006073. RRD2.

Phylogenomic databases

eggNOGi COG5057.
HOGENOMi HOG000205736.
KOi K17605.
OMAi FRTFYDK.
OrthoDBi EOG76HQD0.

Enzyme and pathway databases

BioCyci YEAST:G3O-34049-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12461.
NextBioi 980732.
PROi Q12461.

Gene expression databases

Genevestigatori Q12461.

Family and domain databases

InterProi IPR004327. Phstyr_phstse_ac.
[Graphical view ]
PANTHERi PTHR10012. PTHR10012. 1 hit.
Pfami PF03095. PTPA. 1 hit.
[Graphical view ]
PIRSFi PIRSF016325. Phstyr_phstse_ac. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutations."
    Van Hoof C., Janssens V., De Baere I., Stark M.J.R., de Winde J.H., Winderickx J., Thevelein J.M., Merlevede W., Goris J.
    Exp. Cell Res. 264:372-387(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae."
    Mitchell D.A., Sprague G.F. Jr.
    Mol. Cell. Biol. 21:488-500(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo."
    Fellner T., Lackner D.H., Hombauer H., Piribauer P., Mudrak I., Zaragoza K., Juno C., Ogris E.
    Genes Dev. 17:2138-2150(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
    Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
    Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21 AND PPH22.
  10. "The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes."
    Zheng Y., Jiang Y.
    Mol. Biol. Cell 16:2119-2127(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21 AND TAP42.
  11. "The protein phosphatase 2A phosphatase activator is a novel peptidyl-prolyl cis/trans-isomerase."
    Jordens J., Janssens V., Longin S., Stevens I., Martens E., Bultynck G., Engelborghs Y., Lescrinier E., Waelkens E., Goris J., Van Hoof C.
    J. Biol. Chem. 281:6349-6357(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPTPA2_YEAST
AccessioniPrimary (citable) accession number: Q12461
Secondary accession number(s): D6W3L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2430 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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