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Protein

Serine/threonine-protein phosphatase 2A activator 2

Gene

RRD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for TAP42-associated PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg2+ by dissociating the inactive form from the complex. Acts also inhibitory at high concentrations. Involved in the regulation of cell cycle progression, mitotic spindle formation and bud morphogenesis.6 Publications

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

  • peptidyl-prolyl cis-trans isomerase activity Source: SGD
  • phosphatase activator activity Source: InterPro
  • protein phosphatase type 2A regulator activity Source: SGD

GO - Biological processi

  • mitotic spindle organization in nucleus Source: SGD
  • protein peptidyl-prolyl isomerization Source: GOC
  • regulation of protein phosphatase type 2A activity Source: GOC
  • response to osmotic stress Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Rotamase

Enzyme and pathway databases

BioCyciYEAST:G3O-34049-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2A activator 2 (EC:5.2.1.8)
Alternative name(s):
Peptidyl-prolyl cis-trans isomerase PTPA-2
Short name:
PPIase PTPA-2
Short name:
Rotamase PTPA-2
Phosphotyrosyl phosphatase activator 2
Gene namesi
Name:RRD2
Synonyms:NOH1, YPA2
Ordered Locus Names:YPL152W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPL152w.
EuPathDBiFungiDB:YPL152W.
SGDiS000006073. RRD2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: GOC
  • protein phosphatase type 2A complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Serine/threonine-protein phosphatase 2A activator 2PRO_0000226120Add
BLAST

Proteomic databases

MaxQBiQ12461.
PaxDbiQ12461.
PeptideAtlasiQ12461.

Interactioni

Subunit structurei

Interacts with the phosphatase PP2A catalytic subunits PPH21 and PPH22. Forms a ternary complex with PPH21-TAP42.3 Publications

Protein-protein interaction databases

BioGridi36031. 65 interactions.
DIPiDIP-2558N.
IntActiQ12461. 3 interactions.
MINTiMINT-556521.
STRINGi4932.YPL152W.

Structurei

Secondary structure

1
358
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni10 – 134Combined sources
Helixi14 – 174Combined sources
Helixi20 – 3415Combined sources
Turni35 – 384Combined sources
Helixi50 – 6617Combined sources
Helixi84 – 10320Combined sources
Helixi109 – 12012Combined sources
Turni126 – 1294Combined sources
Helixi133 – 14816Combined sources
Helixi154 – 16310Combined sources
Helixi165 – 18016Combined sources
Beta strandi192 – 1965Combined sources
Helixi199 – 2079Combined sources
Turni208 – 2103Combined sources
Helixi216 – 2205Combined sources
Helixi222 – 2287Combined sources
Turni229 – 2313Combined sources
Helixi233 – 24412Combined sources
Turni245 – 2473Combined sources
Helixi250 – 2534Combined sources
Helixi255 – 2606Combined sources
Helixi266 – 28015Combined sources
Turni281 – 2833Combined sources
Helixi285 – 2884Combined sources
Beta strandi295 – 2973Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IXNX-ray2.80A/B1-304[»]
ProteinModelPortaliQ12461.
SMRiQ12461. Positions 3-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12461.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG5057.
GeneTreeiENSGT00790000123776.
HOGENOMiHOG000205736.
InParanoidiQ12461.
KOiK17605.
OMAiFRTFYDK.
OrthoDBiEOG76HQD0.

Family and domain databases

InterProiIPR004327. Phstyr_phstse_ac.
[Graphical view]
PANTHERiPTHR10012. PTHR10012. 1 hit.
PfamiPF03095. PTPA. 1 hit.
[Graphical view]
PIRSFiPIRSF016325. Phstyr_phstse_ac. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPEKRLLTP DDMKLWEESP TRAHFTKFII DLAESVKGHE NSQYKEPISE
60 70 80 90 100
SINSMMNLLS QIKDITQKHP VIKDADSSRF GKVEFRDFYD EVSRNSRKIL
110 120 130 140 150
RSEFPSLTDE QLEQLSIYLD ESWGNKRRID YGSGHELNFM CLLYGLYSYG
160 170 180 190 200
IFNLSNDSTN LVLKVFIEYL KIMRILETKY WLEPAGSHGV WGLDDYHFLP
210 220 230 240 250
FLFGAFQLTT HKHLKPISIH NNELVEMFAH RYLYFGCIAF INKVKSSASL
260 270 280 290 300
RWHSPMLDDI SGVKTWSKVA EGMIKMYKAE VLSKLPIMQH FYFSEFLPCP
310 320 330 340 350
DGVSPPRGHI HDGTDKDDEC NFEGHVHTTW GDCCGIKLPS AIAATEMNKK

HHKPIPFD
Length:358
Mass (Da):41,452
Last modified:November 1, 1996 - v1
Checksum:iE0BE8A7EDD54E306
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65569.1.
Z73508 Genomic DNA. Translation: CAA97857.1.
BK006949 Genomic DNA. Translation: DAA11283.1.
PIRiS65163.
RefSeqiNP_015173.1. NM_001183966.1.

Genome annotation databases

EnsemblFungiiYPL152W; YPL152W; YPL152W.
GeneIDi855951.
KEGGisce:YPL152W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65569.1.
Z73508 Genomic DNA. Translation: CAA97857.1.
BK006949 Genomic DNA. Translation: DAA11283.1.
PIRiS65163.
RefSeqiNP_015173.1. NM_001183966.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IXNX-ray2.80A/B1-304[»]
ProteinModelPortaliQ12461.
SMRiQ12461. Positions 3-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36031. 65 interactions.
DIPiDIP-2558N.
IntActiQ12461. 3 interactions.
MINTiMINT-556521.
STRINGi4932.YPL152W.

Proteomic databases

MaxQBiQ12461.
PaxDbiQ12461.
PeptideAtlasiQ12461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL152W; YPL152W; YPL152W.
GeneIDi855951.
KEGGisce:YPL152W.

Organism-specific databases

CYGDiYPL152w.
EuPathDBiFungiDB:YPL152W.
SGDiS000006073. RRD2.

Phylogenomic databases

eggNOGiCOG5057.
GeneTreeiENSGT00790000123776.
HOGENOMiHOG000205736.
InParanoidiQ12461.
KOiK17605.
OMAiFRTFYDK.
OrthoDBiEOG76HQD0.

Enzyme and pathway databases

BioCyciYEAST:G3O-34049-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12461.
NextBioi980732.
PROiQ12461.

Family and domain databases

InterProiIPR004327. Phstyr_phstse_ac.
[Graphical view]
PANTHERiPTHR10012. PTHR10012. 1 hit.
PfamiPF03095. PTPA. 1 hit.
[Graphical view]
PIRSFiPIRSF016325. Phstyr_phstse_ac. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutations."
    Van Hoof C., Janssens V., De Baere I., Stark M.J.R., de Winde J.H., Winderickx J., Thevelein J.M., Merlevede W., Goris J.
    Exp. Cell Res. 264:372-387(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae."
    Mitchell D.A., Sprague G.F. Jr.
    Mol. Cell. Biol. 21:488-500(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo."
    Fellner T., Lackner D.H., Hombauer H., Piribauer P., Mudrak I., Zaragoza K., Juno C., Ogris E.
    Genes Dev. 17:2138-2150(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
    Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
    Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21 AND PPH22.
  10. "The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes."
    Zheng Y., Jiang Y.
    Mol. Biol. Cell 16:2119-2127(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PPH21 AND TAP42.
  11. "The protein phosphatase 2A phosphatase activator is a novel peptidyl-prolyl cis/trans-isomerase."
    Jordens J., Janssens V., Longin S., Stevens I., Martens E., Bultynck G., Engelborghs Y., Lescrinier E., Waelkens E., Goris J., Van Hoof C.
    J. Biol. Chem. 281:6349-6357(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPTPA2_YEAST
AccessioniPrimary (citable) accession number: Q12461
Secondary accession number(s): D6W3L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2430 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.