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Q12461

- PTPA2_YEAST

UniProt

Q12461 - PTPA2_YEAST

Protein

Serine/threonine-protein phosphatase 2A activator 2

Gene

RRD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for TAP42-associated PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg2+ by dissociating the inactive form from the complex. Acts also inhibitory at high concentrations. Involved in the regulation of cell cycle progression, mitotic spindle formation and bud morphogenesis.6 Publications

    Catalytic activityi

    Peptidylproline (omega=180) = peptidylproline (omega=0).

    GO - Molecular functioni

    1. peptidyl-prolyl cis-trans isomerase activity Source: SGD
    2. phosphatase activator activity Source: InterPro
    3. protein phosphatase type 2A regulator activity Source: SGD

    GO - Biological processi

    1. mitotic spindle organization in nucleus Source: SGD
    2. protein folding Source: UniProtKB-KW
    3. protein peptidyl-prolyl isomerization Source: GOC
    4. regulation of catalytic activity Source: GOC
    5. response to osmotic stress Source: SGD

    Keywords - Molecular functioni

    Isomerase, Rotamase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34049-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2A activator 2 (EC:5.2.1.8)
    Alternative name(s):
    Peptidyl-prolyl cis-trans isomerase PTPA-2
    Short name:
    PPIase PTPA-2
    Short name:
    Rotamase PTPA-2
    Phosphotyrosyl phosphatase activator 2
    Gene namesi
    Name:RRD2
    Synonyms:NOH1, YPA2
    Ordered Locus Names:YPL152W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL152w.
    SGDiS000006073. RRD2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. protein phosphatase type 2A complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Serine/threonine-protein phosphatase 2A activator 2PRO_0000226120Add
    BLAST

    Proteomic databases

    MaxQBiQ12461.
    PaxDbiQ12461.
    PeptideAtlasiQ12461.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12461.

    Interactioni

    Subunit structurei

    Interacts with the phosphatase PP2A catalytic subunits PPH21 and PPH22. Forms a ternary complex with PPH21-TAP42.3 Publications

    Protein-protein interaction databases

    BioGridi36031. 63 interactions.
    DIPiDIP-2558N.
    IntActiQ12461. 3 interactions.
    MINTiMINT-556521.
    STRINGi4932.YPL152W.

    Structurei

    Secondary structure

    1
    358
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni10 – 134
    Helixi14 – 174
    Helixi20 – 3415
    Turni35 – 384
    Helixi50 – 6617
    Helixi84 – 10320
    Helixi109 – 12012
    Turni126 – 1294
    Helixi133 – 14816
    Helixi154 – 16310
    Helixi165 – 18016
    Beta strandi192 – 1965
    Helixi199 – 2079
    Turni208 – 2103
    Helixi216 – 2205
    Helixi222 – 2287
    Turni229 – 2313
    Helixi233 – 24412
    Turni245 – 2473
    Helixi250 – 2534
    Helixi255 – 2606
    Helixi266 – 28015
    Turni281 – 2833
    Helixi285 – 2884
    Beta strandi295 – 2973

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2IXNX-ray2.80A/B1-304[»]
    ProteinModelPortaliQ12461.
    SMRiQ12461. Positions 3-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12461.

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG5057.
    HOGENOMiHOG000205736.
    KOiK17605.
    OMAiFRTFYDK.
    OrthoDBiEOG76HQD0.

    Family and domain databases

    InterProiIPR004327. Phstyr_phstse_ac.
    [Graphical view]
    PANTHERiPTHR10012. PTHR10012. 1 hit.
    PfamiPF03095. PTPA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016325. Phstyr_phstse_ac. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12461-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPEKRLLTP DDMKLWEESP TRAHFTKFII DLAESVKGHE NSQYKEPISE    50
    SINSMMNLLS QIKDITQKHP VIKDADSSRF GKVEFRDFYD EVSRNSRKIL 100
    RSEFPSLTDE QLEQLSIYLD ESWGNKRRID YGSGHELNFM CLLYGLYSYG 150
    IFNLSNDSTN LVLKVFIEYL KIMRILETKY WLEPAGSHGV WGLDDYHFLP 200
    FLFGAFQLTT HKHLKPISIH NNELVEMFAH RYLYFGCIAF INKVKSSASL 250
    RWHSPMLDDI SGVKTWSKVA EGMIKMYKAE VLSKLPIMQH FYFSEFLPCP 300
    DGVSPPRGHI HDGTDKDDEC NFEGHVHTTW GDCCGIKLPS AIAATEMNKK 350
    HHKPIPFD 358
    Length:358
    Mass (Da):41,452
    Last modified:November 1, 1996 - v1
    Checksum:iE0BE8A7EDD54E306
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96770 Genomic DNA. Translation: CAA65569.1.
    Z73508 Genomic DNA. Translation: CAA97857.1.
    BK006949 Genomic DNA. Translation: DAA11283.1.
    PIRiS65163.
    RefSeqiNP_015173.1. NM_001183966.1.

    Genome annotation databases

    EnsemblFungiiYPL152W; YPL152W; YPL152W.
    GeneIDi855951.
    KEGGisce:YPL152W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96770 Genomic DNA. Translation: CAA65569.1 .
    Z73508 Genomic DNA. Translation: CAA97857.1 .
    BK006949 Genomic DNA. Translation: DAA11283.1 .
    PIRi S65163.
    RefSeqi NP_015173.1. NM_001183966.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2IXN X-ray 2.80 A/B 1-304 [» ]
    ProteinModelPortali Q12461.
    SMRi Q12461. Positions 3-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36031. 63 interactions.
    DIPi DIP-2558N.
    IntActi Q12461. 3 interactions.
    MINTi MINT-556521.
    STRINGi 4932.YPL152W.

    Proteomic databases

    MaxQBi Q12461.
    PaxDbi Q12461.
    PeptideAtlasi Q12461.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL152W ; YPL152W ; YPL152W .
    GeneIDi 855951.
    KEGGi sce:YPL152W.

    Organism-specific databases

    CYGDi YPL152w.
    SGDi S000006073. RRD2.

    Phylogenomic databases

    eggNOGi COG5057.
    HOGENOMi HOG000205736.
    KOi K17605.
    OMAi FRTFYDK.
    OrthoDBi EOG76HQD0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34049-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12461.
    NextBioi 980732.
    PROi Q12461.

    Gene expression databases

    Genevestigatori Q12461.

    Family and domain databases

    InterProi IPR004327. Phstyr_phstse_ac.
    [Graphical view ]
    PANTHERi PTHR10012. PTHR10012. 1 hit.
    Pfami PF03095. PTPA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016325. Phstyr_phstse_ac. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
      Purnelle B., Coster F., Goffeau A.
      Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutations."
      Van Hoof C., Janssens V., De Baere I., Stark M.J.R., de Winde J.H., Winderickx J., Thevelein J.M., Merlevede W., Goris J.
      Exp. Cell Res. 264:372-387(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae."
      Mitchell D.A., Sprague G.F. Jr.
      Mol. Cell. Biol. 21:488-500(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo."
      Fellner T., Lackner D.H., Hombauer H., Piribauer P., Mudrak I., Zaragoza K., Juno C., Ogris E.
      Genes Dev. 17:2138-2150(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PPH21.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
      Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
      Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PPH21 AND PPH22.
    10. "The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes."
      Zheng Y., Jiang Y.
      Mol. Biol. Cell 16:2119-2127(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PPH21 AND TAP42.
    11. "The protein phosphatase 2A phosphatase activator is a novel peptidyl-prolyl cis/trans-isomerase."
      Jordens J., Janssens V., Longin S., Stevens I., Martens E., Bultynck G., Engelborghs Y., Lescrinier E., Waelkens E., Goris J., Van Hoof C.
      J. Biol. Chem. 281:6349-6357(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPTPA2_YEAST
    AccessioniPrimary (citable) accession number: Q12461
    Secondary accession number(s): D6W3L7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2430 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3