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Protein

Nucleolar protein 56

Gene

NOP56

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for 60S ribosomal subunit synthesis.1 Publication

GO - Biological processi

  • rRNA modification Source: SGD
  • rRNA processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciYEAST:G3O-32318-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar protein 56
Alternative name(s):
Ribosome biosynthesis protein SIK1
Suppressor of I kappa b protein 1
Gene namesi
Name:NOP56
Synonyms:SIK1
Ordered Locus Names:YLR197W
ORF Names:L8167.9
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR197W.
SGDiS000004187. NOP56.

Subcellular locationi

  • Nucleusnucleolus 1 Publication

GO - Cellular componenti

  • box C/D snoRNP complex Source: SGD
  • nucleolus Source: SGD
  • small-subunit processome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi333 – 3331V → A: Reduced growth rate at all temperatures; when associated with R-385. 1 Publication
Mutagenesisi355 – 3551Y → C: At 37 degrees, growth slows after 6 to 8 hours and cell division stops after 20 hours. 1 Publication
Mutagenesisi385 – 3851M → R: Reduced growth rate at all temperatures; when associated with A-333. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Nucleolar protein 56PRO_0000219030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei321 – 3211PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12460.
PeptideAtlasiQ12460.

PTM databases

iPTMnetiQ12460.

Interactioni

Subunit structurei

Interacts with NOP1 and NOP58. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.2 Publications

Protein-protein interaction databases

BioGridi31467. 136 interactions.
DIPiDIP-4405N.
IntActiQ12460. 33 interactions.
MINTiMINT-525679.

Structurei

3D structure databases

ProteinModelPortaliQ12460.
SMRiQ12460. Positions 158-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini299 – 417119NopPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi443 – 50462Asp/Glu/Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the NOP5/NOP56 family.Curated
Contains 1 Nop domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075068.
HOGENOMiHOG000196309.
InParanoidiQ12460.
KOiK14564.
OMAiMETNMPE.
OrthoDBiEOG7C5MJW.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPIEYLLFE EPTGYAVFKV KLQQDDIGSR LKEVQEQIND FGAFTKLIEL
60 70 80 90 100
VSFAPFKGAA EALENANDIS EGLVSESLKA ILDLNLPKAS SKKKNITLAI
110 120 130 140 150
SDKNLGPSIK EEFPYVDCIS NELAQDLIRG VRLHGEKLFK GLQSGDLERA
160 170 180 190 200
QLGLGHAYSR AKVKFSVQKN DNHIIQAIAL LDQLDKDINT FAMRVKEWYG
210 220 230 240 250
WHFPELAKLV PDNYTFAKLV LFIKDKASLN DDSLHDLAAL LNEDSGIAQR
260 270 280 290 300
VIDNARISMG QDISETDMEN VCVFAQRVAS LADYRRQLYD YLCEKMHTVA
310 320 330 340 350
PNLSELIGEV IGARLISHAG SLTNLSKQAA STVQILGAEK ALFRALKTKG
360 370 380 390 400
NTPKYGLIYH SGFISKASAK NKGRISRYLA NKCSMASRID NYSEEPSNVF
410 420 430 440 450
GSVLKKQVEQ RLEFYNTGKP TLKNELAIQE AMELYNKDKP AAEVEETKEK
460 470 480 490 500
ESSKKRKLED DDEEKKEKKE KKSKKEKKEK KEKKDKKEKK DKKEKKDKKK

KSKD
Length:504
Mass (Da):56,864
Last modified:November 1, 1996 - v1
Checksum:iF8522A5870EF4842
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20237 Genomic DNA. Translation: AAC49066.1.
U14913 Genomic DNA. Translation: AAB67431.1.
BK006945 Genomic DNA. Translation: DAA09516.1.
PIRiS48550.
RefSeqiNP_013298.1. NM_001182084.1.

Genome annotation databases

EnsemblFungiiYLR197W; YLR197W; YLR197W.
GeneIDi850894.
KEGGisce:YLR197W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20237 Genomic DNA. Translation: AAC49066.1.
U14913 Genomic DNA. Translation: AAB67431.1.
BK006945 Genomic DNA. Translation: DAA09516.1.
PIRiS48550.
RefSeqiNP_013298.1. NM_001182084.1.

3D structure databases

ProteinModelPortaliQ12460.
SMRiQ12460. Positions 158-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31467. 136 interactions.
DIPiDIP-4405N.
IntActiQ12460. 33 interactions.
MINTiMINT-525679.

PTM databases

iPTMnetiQ12460.

Proteomic databases

MaxQBiQ12460.
PeptideAtlasiQ12460.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR197W; YLR197W; YLR197W.
GeneIDi850894.
KEGGisce:YLR197W.

Organism-specific databases

EuPathDBiFungiDB:YLR197W.
SGDiS000004187. NOP56.

Phylogenomic databases

GeneTreeiENSGT00550000075068.
HOGENOMiHOG000196309.
InParanoidiQ12460.
KOiK14564.
OMAiMETNMPE.
OrthoDBiEOG7C5MJW.

Enzyme and pathway databases

BioCyciYEAST:G3O-32318-MONOMER.

Miscellaneous databases

NextBioi967272.
PROiQ12460.

Family and domain databases

InterProiIPR012974. NOP5_N.
IPR002687. Nop_dom.
IPR012976. NOSIC.
[Graphical view]
PfamiPF01798. Nop. 1 hit.
PF08156. NOP5NT. 1 hit.
[Graphical view]
SMARTiSM00931. NOSIC. 1 hit.
[Graphical view]
SUPFAMiSSF89124. SSF89124. 1 hit.
PROSITEiPS51358. NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic analysis of growth inhibition by GAL4-L kappa B-alpha in Saccharomyces cerevisiae."
    Morin P.J., Downs J.A., Snodgrass A.M., Gilmore T.D.
    Cell Growth Differ. 6:789-798(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / YPH1.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis."
    Gautier T., Berges T., Tollervey D., Hurt E.
    Mol. Cell. Biol. 17:7088-7098(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NOP1 AND NOP58, SUBCELLULAR LOCATION, MUTAGENESIS OF VAL-333; TYR-355 AND MET-385.
  5. Cited for: IDENTIFICATION IN SSU PROCESSOME BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNOP56_YEAST
AccessioniPrimary (citable) accession number: Q12460
Secondary accession number(s): D6VYK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.