Q12455 (SPSY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermine synthase
Short name=SPMSY
EC=2.5.1.22

Alternative name(s):
Spermidine aminopropyltransferase

Gene names

Name:	SPE4
Ordered Locus Names:	YLR146C
ORF Names:	L9634.5

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	S-adenosylmethioninamine + spermidine = S-methyl-5'-thioadenosine + spermine.
Pathway	Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.
Miscellaneous	Present with 3870 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from mutant phenotype PubMed 11154694. Source: SGD spermine biosynthetic process Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular function	protein binding Inferred from physical interaction PubMed 11805826PubMed 18719252PubMed 16429126. Source: IntAct spermine synthase activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPE3	Q12074	3	EBI-17907,EBI-17836

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Spermine synthase		PRO_0000156540
Regions
	Region	151 – 152	2	S-adenosylmethioninamine binding By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	44	1	S-adenosylmethioninamine By similarity
	Binding site	99	1	S-adenosylmethioninamine By similarity
	Binding site	119	1	S-adenosylmethioninamine By similarity
	Binding site	177	1	Spermidine By similarity
Amino acid modifications
	Modified residue	5	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q12455 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FE7FE6EE308F3CC9	FASTA	300	34,091
	10 20 30 40 50 60 MVNNSQHPYI KDGWFREIND KSFPGQAFTM TVDSILYEAR SEFQDILIFR NKVYGTVLVL 70 80 90 100 110 120 DGIVQCTEFD EFAYQEMITH IAMFAHSNPK RVLIIGGGDG GVLREVAKHS CVEDITMVEI 130 140 150 160 170 180 DSSVIELSRK FLPTLSNGAF DDERLDLKLC DGFKFLQDIG ASDVHKKFDV IITDSSDPEG 190 200 210 220 230 240 PAEAFFQERY FELLKDALNP NGVVIMQSSE NFWLNLKYLH DLKNTAKKVF PNTEYCYTMV 250 260 270 280 290 300 PTYTSGQLGL IVCSNNANIP LNIPQRKISE QEQGKLKYYN PQIHSSAFVL PTWADKVINE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Spermine is not essential for growth of Saccharomyces cerevisiae: identification of the SPE4 gene (spermine synthase) and characterization of a spe4 deletion mutant." Hamasaki-Katagiri N., Katagiri Y., Tabor C.W., Tabor H. Gene 210:195-201(1998) [PubMed: 9573363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 2602.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE." Zhang Z., Dietrich F.S. Nucleic Acids Res. 33:2838-2851(2005) [PubMed: 15905473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-82. Strain: ATCC 208353 / W303-1A.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF067970 Genomic DNA. Translation: AAC19368.1. Z73318 Genomic DNA. Translation: CAA97718.1. U53879 Genomic DNA. Translation: AAB82380.1. AY899255 mRNA. Translation: AAX83940.1. BK006945 Genomic DNA. Translation: DAA09456.1.
PIR	S64995.
RefSeq	NP_013247.1. NM_001182033.1.
3D structure databases
ProteinModelPortal	Q12455.
SMR	Q12455. Positions 5-300.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4811N.
IntAct	Q12455. 2 interactions.
MINT	MINT-475905.
STRING	Q12455.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR146C; YLR146C; YLR146C.
GeneID	850838.
KEGG	sce:YLR146C.
Organism-specific databases
CYGD	YLR146c.
SGD	S000004136. SPE4.
Phylogenomic databases
eggNOG	COG0421.
GeneTree	ENSGT00620000088036.
HOGENOM	HOG000256147.
KO	K00802.
OMA	FAYQELI.
OrthoDB	EOG4QVGN4.
PhylomeDB	Q12455.
Gene expression databases
ArrayExpress	Q12455.
Genevestigator	Q12455.
GermOnline	YLR146C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. SpeE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967117.

Entry information

Entry name

SPSY_YEAST

Accession

Primary (citable) accession number: Q12455
Secondary accession number(s): D6VYE0, Q2VQW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	May 16, 2012
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program