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Protein

Spermine synthase

Gene

SPE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + spermidine = S-methyl-5'-thioadenosine + spermine.

Pathwayi: spermine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermine from spermidine.
Proteins known to be involved in this subpathway in this organism are:
  1. Spermine synthase (SPE4)
This subpathway is part of the pathway spermine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermine from spermidine, the pathway spermine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosylmethioninamineBy similarity
Binding sitei99 – 991S-adenosylmethioninamineBy similarity
Binding sitei119 – 1191S-adenosylmethioninamineBy similarity
Active sitei174 – 1741Proton acceptorBy similarity
Binding sitei177 – 1771SpermidineBy similarity

GO - Molecular functioni

  • spermine synthase activity Source: SGD

GO - Biological processi

  • pantothenate biosynthetic process Source: SGD
  • spermine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YLR146C-MONOMER.
YEAST:YLR146C-MONOMER.
ReactomeiR-SCE-351202. Metabolism of polyamines.
UniPathwayiUPA00249; UER00315.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermine synthase (EC:2.5.1.22)
Short name:
SPMSY
Alternative name(s):
Spermidine aminopropyltransferase
Gene namesi
Name:SPE4
Ordered Locus Names:YLR146C
ORF Names:L9634.5
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR146C.
SGDiS000004136. SPE4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Spermine synthasePRO_0000156540Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12455.

PTM databases

iPTMnetiQ12455.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SPE3Q120743EBI-17907,EBI-17836

Protein-protein interaction databases

BioGridi31415. 33 interactions.
DIPiDIP-4811N.
IntActiQ12455. 2 interactions.
MINTiMINT-475905.

Structurei

3D structure databases

ProteinModelPortaliQ12455.
SMRiQ12455. Positions 10-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 255244PABSAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni151 – 1522S-adenosylmethioninamine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00620000088036.
HOGENOMiHOG000256147.
InParanoidiQ12455.
KOiK00802.
OMAiGIVIMQS.
OrthoDBiEOG092C2J3O.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
IPR030668. Spermi_synthase_euk.
[Graphical view]
PIRSFiPIRSF000502. Spermidine_synth. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12455-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNNSQHPYI KDGWFREIND KSFPGQAFTM TVDSILYEAR SEFQDILIFR
60 70 80 90 100
NKVYGTVLVL DGIVQCTEFD EFAYQEMITH IAMFAHSNPK RVLIIGGGDG
110 120 130 140 150
GVLREVAKHS CVEDITMVEI DSSVIELSRK FLPTLSNGAF DDERLDLKLC
160 170 180 190 200
DGFKFLQDIG ASDVHKKFDV IITDSSDPEG PAEAFFQERY FELLKDALNP
210 220 230 240 250
NGVVIMQSSE NFWLNLKYLH DLKNTAKKVF PNTEYCYTMV PTYTSGQLGL
260 270 280 290 300
IVCSNNANIP LNIPQRKISE QEQGKLKYYN PQIHSSAFVL PTWADKVINE
Length:300
Mass (Da):34,091
Last modified:November 1, 1996 - v1
Checksum:iFE7FE6EE308F3CC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067970 Genomic DNA. Translation: AAC19368.1.
Z73318 Genomic DNA. Translation: CAA97718.1.
U53879 Genomic DNA. Translation: AAB82380.1.
AY899255 mRNA. Translation: AAX83940.1.
BK006945 Genomic DNA. Translation: DAA09456.1.
PIRiS64995.
RefSeqiNP_013247.1. NM_001182033.1.

Genome annotation databases

EnsemblFungiiYLR146C; YLR146C; YLR146C.
GeneIDi850838.
KEGGisce:YLR146C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067970 Genomic DNA. Translation: AAC19368.1.
Z73318 Genomic DNA. Translation: CAA97718.1.
U53879 Genomic DNA. Translation: AAB82380.1.
AY899255 mRNA. Translation: AAX83940.1.
BK006945 Genomic DNA. Translation: DAA09456.1.
PIRiS64995.
RefSeqiNP_013247.1. NM_001182033.1.

3D structure databases

ProteinModelPortaliQ12455.
SMRiQ12455. Positions 10-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31415. 33 interactions.
DIPiDIP-4811N.
IntActiQ12455. 2 interactions.
MINTiMINT-475905.

PTM databases

iPTMnetiQ12455.

Proteomic databases

MaxQBiQ12455.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR146C; YLR146C; YLR146C.
GeneIDi850838.
KEGGisce:YLR146C.

Organism-specific databases

EuPathDBiFungiDB:YLR146C.
SGDiS000004136. SPE4.

Phylogenomic databases

GeneTreeiENSGT00620000088036.
HOGENOMiHOG000256147.
InParanoidiQ12455.
KOiK00802.
OMAiGIVIMQS.
OrthoDBiEOG092C2J3O.

Enzyme and pathway databases

UniPathwayiUPA00249; UER00315.
BioCyciMetaCyc:YLR146C-MONOMER.
YEAST:YLR146C-MONOMER.
ReactomeiR-SCE-351202. Metabolism of polyamines.

Miscellaneous databases

PROiQ12455.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
IPR030668. Spermi_synthase_euk.
[Graphical view]
PIRSFiPIRSF000502. Spermidine_synth. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPSY_YEAST
AccessioniPrimary (citable) accession number: Q12455
Secondary accession number(s): D6VYE0, Q2VQW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.