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Q12446 (LAS17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline-rich protein LAS17
Gene names
Name:LAS17
Ordered Locus Names:YOR181W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length633 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with KRE6, LSB3, LSB5 and YSC84. Ref.4 Ref.5

Miscellaneous

Present with 8580 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 WH1 domain.

Contains 1 WH2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 633633Proline-rich protein LAS17
PRO_0000084361

Regions

Domain16 – 127112WH1
Domain547 – 56721WH2
Compositional bias185 – 529345Pro-rich
Compositional bias185 – 1906Poly-Pro
Compositional bias323 – 3297Poly-Pro
Compositional bias342 – 3487Poly-Pro
Compositional bias352 – 3587Poly-Pro
Compositional bias385 – 3917Poly-Pro
Compositional bias427 – 4315Poly-Pro
Compositional bias470 – 4745Poly-Pro
Compositional bias503 – 5075Poly-Pro
Compositional bias520 – 5267Poly-Pro

Amino acid modifications

Modified residue3341Phosphothreonine Ref.8
Modified residue3371Phosphoserine Ref.8
Modified residue5881Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q12446 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 4488355563AA2645

FASTA63367,572
        10         20         30         40         50         60 
MGLLNSSDKE IIKRALPKAS NKIIDVTVAR LYIAYPDKNE WQYTGLSGAL ALVDDLVGNT 

        70         80         90        100        110        120 
FFLKLVDING HRGVIWDQEL YVNFEYYQDR TFFHTFEMEE CFAGLLFVDI NEASHFLKRV 

       130        140        150        160        170        180 
QKRERYANRK TLLNKNAVAL TKKVREEQKS QVVHGPRGES LIDNQRKRYN YEDVDTIPTT 

       190        200        210        220        230        240 
KHKAPPPPPP TAETFDSDQT SSFSDINSTT ASAPTTPAPA LPPASPEVRK EETHPKHSLP 

       250        260        270        280        290        300 
PLPNQFAPLP DPPQHNSPPQ NNAPSQPQSN PFPFPIPEIP STQSATNPFP FPVPQQQFNQ 

       310        320        330        340        350        360 
APSMGIPQQN RPLPQLPNRN NRPVPPPPPM RTTTEGSGVR LPAPPPPPRR GPAPPPPPHR 

       370        380        390        400        410        420 
HVTSNTLNSA GGNSLLPQAT GRRGPAPPPP PRASRPTPNV TMQQNPQQYN NSNRPFGYQT 

       430        440        450        460        470        480 
NSNMSSPPPP PVTTFNTLTP QMTAATGQPA VPLPQNTQAP SQATNVPVAP PPPPASLGQS 

       490        500        510        520        530        540 
QIPQSAPSAP IPPTLPSTTS AAPPPPPAFL TQQPQSGGAP APPPPPQMPA TSTSGGGSFA 

       550        560        570        580        590        600 
ETTGDAGRDA LLASIRGAGG IGALRKVDKS QLDKPSVLLQ EARGESASPP AAAGNGGTPG 

       610        620        630 
GPPASLADAL AAALNKRKTK VGAHDDMDNG DDW 

« Hide

References

« Hide 'large scale' references
[1]"Yeast mutants sensitive to local anesthetics."
Toh-e A.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LSB3; LSB5 AND YSC84.
[5]"Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p."
Li H., Page N., Bussey H.
Yeast 19:1097-1112(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KRE6.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-334 AND SER-337, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78487 Genomic DNA. Translation: BAA11386.1.
Z75089 Genomic DNA. Translation: CAA99390.1.
BK006948 Genomic DNA. Translation: DAA10953.1.
PIRS62057.
RefSeqNP_014824.1. NM_001183600.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZUKX-ray1.90C350-360[»]
ProteinModelPortalQ12446.
SMRQ12446. Positions 28-120.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34576. 156 interactions.
DIPDIP-963N.
IntActQ12446. 38 interactions.
MINTMINT-370148.
STRING4932.YOR181W.

Proteomic databases

MaxQBQ12446.
PaxDbQ12446.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR181W; YOR181W; YOR181W.
GeneID854353.
KEGGsce:YOR181W.

Organism-specific databases

CYGDYOR181w.
SGDS000005707. LAS17.

Phylogenomic databases

eggNOGNOG270974.
GeneTreeENSGT00730000110895.
HOGENOMHOG000111174.
OMADTNDATH.
OrthoDBEOG71K6CX.

Enzyme and pathway databases

BioCycYEAST:G3O-33692-MONOMER.

Gene expression databases

GenevestigatorQ12446.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR000697. WH1/EVH1.
IPR003124. WH2_dom.
IPR028297. Wsp1/Las17_fungal.
[Graphical view]
PANTHERPTHR23202:SF2. PTHR23202:SF2. 1 hit.
PfamPF00568. WH1. 1 hit.
PF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00461. WH1. 1 hit.
SM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS50229. WH1. 1 hit.
PS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ12446.
NextBio976446.
PROQ12446.

Entry information

Entry nameLAS17_YEAST
AccessionPrimary (citable) accession number: Q12446
Secondary accession number(s): D6W2N7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references