Reviewed,
UniProtKB/Swiss-Prot Q12446 (LAS17_YEAST)
Last modified
June 16, 2009.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Proline-rich protein LAS17 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 633 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Subunit structure | |
| Miscellaneous | Present with 8580 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Contains 1 WH1 domain. Contains 1 WH2 domain. |
Ontologies
| Keywords | |
|---|---|
| PTM | Phosphoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | actin cortical patch localization Inferred from genetic interaction. Source: SGD actin polymerization or depolymerizationInferred from direct assay. Source: SGD bipolar cellular bud site selectionTraceable author statement. Source: SGD endocytosisTraceable author statement. Source: SGD positive regulation of actin filament bundle formationInferred from direct assay. Source: SGD response to osmotic stressTraceable author statement. Source: SGD |
| Cellular component | actin cortical patch Ref.3 Inferred from direct assay. Source: SGD mating projection tipInferred from direct assay. Source: SGD |
| Molecular function | cytoskeletal protein binding Inferred from direct assay. Source: SGD identical protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-10022,EBI-10022 | ||
| ACT1 | P60010 | 2 | EBI-10022,EBI-2169 | |
| ARC15 | P40518 | 1 | EBI-10022,EBI-2750 | |
| ARC18 | Q05933 | 1 | EBI-10022,EBI-2764 | |
| ARC19 | P33204 | 1 | EBI-10022,EBI-2757 | |
| ARC35 | P53731 | 1 | EBI-10022,EBI-2770 | |
| ARC40 | P38328 | 2 | EBI-10022,EBI-2777 | |
| ARP2 | P32381 | 2 | EBI-10022,EBI-2927 | |
| BBC1 | P47068 | 2 | EBI-10022,EBI-3437 | |
| BZZ1 | P38822 | 6 | EBI-10022,EBI-3889 | |
| CYK2 | Q05080 | 2 | EBI-10022,EBI-5412 | |
| END3 | P39013 | 1 | EBI-10022,EBI-6460 | |
| LSB1 | P53281 | 5 | EBI-10022,EBI-23329 | |
| LSB3 | P43603 | 6 | EBI-10022,EBI-22980 | |
| LSB5 | P25369 | 4 | EBI-10022,EBI-10218 | |
| MYO3 | P36006 | 2 | EBI-10022,EBI-11670 | |
| MYO5 | Q04439 | 4 | EBI-10022,EBI-11687 | |
| PIN3 | Q06449 | 5 | EBI-10022,EBI-35523 | |
| RVS167 | P39743 | 6 | EBI-10022,EBI-14500 | |
| SLA2 | P33338 | 1 | EBI-10022,EBI-17323 | |
| SSU81 | P40073 | 2 | EBI-10022,EBI-18140 | |
| SYP1 | P25623 | 1 | EBI-10022,EBI-21900 | |
| VRP1 | P37370 | 3 | EBI-10022,EBI-20502 | |
| YSC84 | P32793 | 7 | EBI-10022,EBI-24460 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 633 | 633 | Proline-rich protein LAS17 | PRO_0000084361 | |||||
Regions | |||||||||
| Domain | 16 – 127 | 112 | WH1 | ||||||
| Domain | 547 – 567 | 21 | WH2 | ||||||
| Compositional bias | 185 – 529 | 345 | Pro-rich | ||||||
| Compositional bias | 185 – 190 | 6 | Poly-Pro | ||||||
| Compositional bias | 323 – 329 | 7 | Poly-Pro | ||||||
| Compositional bias | 342 – 348 | 7 | Poly-Pro | ||||||
| Compositional bias | 352 – 358 | 7 | Poly-Pro | ||||||
| Compositional bias | 385 – 391 | 7 | Poly-Pro | ||||||
| Compositional bias | 427 – 431 | 5 | Poly-Pro | ||||||
| Compositional bias | 470 – 474 | 5 | Poly-Pro | ||||||
| Compositional bias | 503 – 507 | 5 | Poly-Pro | ||||||
| Compositional bias | 520 – 526 | 7 | Poly-Pro | ||||||
Amino acid modifications | |||||||||
| Modified residue | 586 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 588 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Yeast mutants sensitive to local anesthetics." Toh-e A. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K.Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex." Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B. Mol. Biol. Cell 10:3521-3538(1999) [PubMed: 10512884] [Abstract] Cited for: INTERACTION WITH LSB3; LSB5 AND YSC84. |
| [4] | "Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p." Li H., Page N., Bussey H. Yeast 19:1097-1112(2002) [PubMed: 12237851] [Abstract] Cited for: INTERACTION WITH KRE6. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586 AND SER-588, MASS SPECTROMETRY. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D78487 Genomic DNA. Translation: BAA11386.1. Z75089 Genomic DNA. Translation: CAA99390.1. | |||||||||||||
| PIR | S62057. | ||||||||||||
| RefSeq | NP_014824.1. | ||||||||||||
3D structure databases | |||||||||||||
| |||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:963N. | ||||||||||||
| IntAct | Q12446. 53 interactions. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | YOR181W. Saccharomyces cerevisiae. [Contig view] | ||||||||||||
| GeneID | 854353. | ||||||||||||
| GenomeReviews | Gene locus YOR181W in contig Y13140_GR. | ||||||||||||
| KEGG | sce:YOR181W. | ||||||||||||
| NMPDR | fig|4932.3.peg.5932. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YOR181w. | ||||||||||||
| SGD | S000005707. LAS17. | ||||||||||||
| Yeast-GFP | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q12446. | ||||||||||||
| OMA | Q12446. LYIAYPD. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | Q12446. | ||||||||||||
| GermOnline | YOR181W. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000697. EVH1. IPR011993. PH_type. IPR003124. WH2_actin_bd. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. | ||||||||||||
| Pfam | PF00568. WH1. 1 hit. PF02205. WH2. 1 hit. [Graphical view] | ||||||||||||
| SMART | SM00461. WH1. 1 hit. SM00246. WH2. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS50229. WH1. 1 hit. PS51082. WH2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 976446. | ||||||||||||
Entry information
| Entry name | LAS17_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q12446 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


