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Q12446

- LAS17_YEAST

UniProt

Q12446 - LAS17_YEAST

Protein

Proline-rich protein LAS17

Gene

LAS17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. actin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin cortical patch localization Source: SGD
    2. actin filament organization Source: SGD
    3. actin filament polymerization Source: SGD
    4. actin nucleation Source: SGD
    5. endocytosis Source: InterPro
    6. positive regulation of actin filament bundle assembly Source: SGD
    7. positive regulation of Arp2/3 complex-mediated actin nucleation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33692-MONOMER.
    ReactomeiREACT_189214. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline-rich protein LAS17
    Gene namesi
    Name:LAS17
    Ordered Locus Names:YOR181W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR181w.
    SGDiS000005707. LAS17.

    Subcellular locationi

    GO - Cellular componenti

    1. actin cortical patch Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 633633Proline-rich protein LAS17PRO_0000084361Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei334 – 3341Phosphothreonine1 Publication
    Modified residuei337 – 3371Phosphoserine1 Publication
    Modified residuei588 – 5881Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12446.
    PaxDbiQ12446.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12446.

    Interactioni

    Subunit structurei

    Interacts with KRE6, LSB3, LSB5 and YSC84.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARC40P383282EBI-10022,EBI-2777
    BZZ1P3882214EBI-10022,EBI-3889
    END3P390133EBI-10022,EBI-6460
    HOF1Q050803EBI-10022,EBI-5412
    LSB3P436039EBI-10022,EBI-22980
    LSB5P253693EBI-10022,EBI-10218
    MYO3P360063EBI-10022,EBI-11670
    MYO5Q044397EBI-10022,EBI-11687
    PEX13P806672EBI-10022,EBI-13206
    PIN3Q064494EBI-10022,EBI-35523
    RVS167P3974312EBI-10022,EBI-14500
    SHO1P400735EBI-10022,EBI-18140
    SLA1P327906EBI-10022,EBI-17313
    SLA2P333383EBI-10022,EBI-17323
    VRP1P373706EBI-10022,EBI-20502
    YSC84P327937EBI-10022,EBI-24460

    Protein-protein interaction databases

    BioGridi34576. 156 interactions.
    DIPiDIP-963N.
    IntActiQ12446. 38 interactions.
    MINTiMINT-370148.
    STRINGi4932.YOR181W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZUKX-ray1.90C350-360[»]
    ProteinModelPortaliQ12446.
    SMRiQ12446. Positions 28-120.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12446.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 127112WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini547 – 56721WH2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi185 – 529345Pro-richAdd
    BLAST
    Compositional biasi185 – 1906Poly-Pro
    Compositional biasi323 – 3297Poly-Pro
    Compositional biasi342 – 3487Poly-Pro
    Compositional biasi352 – 3587Poly-Pro
    Compositional biasi385 – 3917Poly-Pro
    Compositional biasi427 – 4315Poly-Pro
    Compositional biasi470 – 4745Poly-Pro
    Compositional biasi503 – 5075Poly-Pro
    Compositional biasi520 – 5267Poly-Pro

    Sequence similaritiesi

    Contains 1 WH1 domain.PROSITE-ProRule annotation
    Contains 1 WH2 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG270974.
    GeneTreeiENSGT00730000110895.
    HOGENOMiHOG000111174.
    OMAiDTNDATH.
    OrthoDBiEOG71K6CX.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR011993. PH_like_dom.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    IPR028297. Wsp1/Las17_fungal.
    [Graphical view]
    PANTHERiPTHR23202:SF2. PTHR23202:SF2. 1 hit.
    PfamiPF00568. WH1. 1 hit.
    PF02205. WH2. 1 hit.
    [Graphical view]
    SMARTiSM00461. WH1. 1 hit.
    SM00246. WH2. 1 hit.
    [Graphical view]
    PROSITEiPS50229. WH1. 1 hit.
    PS51082. WH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12446-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLLNSSDKE IIKRALPKAS NKIIDVTVAR LYIAYPDKNE WQYTGLSGAL    50
    ALVDDLVGNT FFLKLVDING HRGVIWDQEL YVNFEYYQDR TFFHTFEMEE 100
    CFAGLLFVDI NEASHFLKRV QKRERYANRK TLLNKNAVAL TKKVREEQKS 150
    QVVHGPRGES LIDNQRKRYN YEDVDTIPTT KHKAPPPPPP TAETFDSDQT 200
    SSFSDINSTT ASAPTTPAPA LPPASPEVRK EETHPKHSLP PLPNQFAPLP 250
    DPPQHNSPPQ NNAPSQPQSN PFPFPIPEIP STQSATNPFP FPVPQQQFNQ 300
    APSMGIPQQN RPLPQLPNRN NRPVPPPPPM RTTTEGSGVR LPAPPPPPRR 350
    GPAPPPPPHR HVTSNTLNSA GGNSLLPQAT GRRGPAPPPP PRASRPTPNV 400
    TMQQNPQQYN NSNRPFGYQT NSNMSSPPPP PVTTFNTLTP QMTAATGQPA 450
    VPLPQNTQAP SQATNVPVAP PPPPASLGQS QIPQSAPSAP IPPTLPSTTS 500
    AAPPPPPAFL TQQPQSGGAP APPPPPQMPA TSTSGGGSFA ETTGDAGRDA 550
    LLASIRGAGG IGALRKVDKS QLDKPSVLLQ EARGESASPP AAAGNGGTPG 600
    GPPASLADAL AAALNKRKTK VGAHDDMDNG DDW 633
    Length:633
    Mass (Da):67,572
    Last modified:November 1, 1997 - v1
    Checksum:i4488355563AA2645
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78487 Genomic DNA. Translation: BAA11386.1.
    Z75089 Genomic DNA. Translation: CAA99390.1.
    BK006948 Genomic DNA. Translation: DAA10953.1.
    PIRiS62057.
    RefSeqiNP_014824.1. NM_001183600.1.

    Genome annotation databases

    EnsemblFungiiYOR181W; YOR181W; YOR181W.
    GeneIDi854353.
    KEGGisce:YOR181W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78487 Genomic DNA. Translation: BAA11386.1 .
    Z75089 Genomic DNA. Translation: CAA99390.1 .
    BK006948 Genomic DNA. Translation: DAA10953.1 .
    PIRi S62057.
    RefSeqi NP_014824.1. NM_001183600.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZUK X-ray 1.90 C 350-360 [» ]
    ProteinModelPortali Q12446.
    SMRi Q12446. Positions 28-120.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34576. 156 interactions.
    DIPi DIP-963N.
    IntActi Q12446. 38 interactions.
    MINTi MINT-370148.
    STRINGi 4932.YOR181W.

    Proteomic databases

    MaxQBi Q12446.
    PaxDbi Q12446.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR181W ; YOR181W ; YOR181W .
    GeneIDi 854353.
    KEGGi sce:YOR181W.

    Organism-specific databases

    CYGDi YOR181w.
    SGDi S000005707. LAS17.

    Phylogenomic databases

    eggNOGi NOG270974.
    GeneTreei ENSGT00730000110895.
    HOGENOMi HOG000111174.
    OMAi DTNDATH.
    OrthoDBi EOG71K6CX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33692-MONOMER.
    Reactomei REACT_189214. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    EvolutionaryTracei Q12446.
    NextBioi 976446.
    PROi Q12446.

    Gene expression databases

    Genevestigatori Q12446.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR011993. PH_like_dom.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    IPR028297. Wsp1/Las17_fungal.
    [Graphical view ]
    PANTHERi PTHR23202:SF2. PTHR23202:SF2. 1 hit.
    Pfami PF00568. WH1. 1 hit.
    PF02205. WH2. 1 hit.
    [Graphical view ]
    SMARTi SM00461. WH1. 1 hit.
    SM00246. WH2. 1 hit.
    [Graphical view ]
    PROSITEi PS50229. WH1. 1 hit.
    PS51082. WH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast mutants sensitive to local anesthetics."
      Toh-e A.
      Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Saccharomyces cerevisiae homologue of human Wiskott-Aldrich syndrome protein Las17p interacts with the Arp2/3 complex."
      Madania A., Dumoulin P., Grava S., Kitamoto H., Scharer-Brodbeck C., Soulard A., Moreau V., Winsor B.
      Mol. Biol. Cell 10:3521-3538(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH LSB3; LSB5 AND YSC84.
    5. "Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p."
      Li H., Page N., Bussey H.
      Yeast 19:1097-1112(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KRE6.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-334 AND SER-337, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiLAS17_YEAST
    AccessioniPrimary (citable) accession number: Q12446
    Secondary accession number(s): D6W2N7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8580 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3