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Q12445

- POM34_YEAST

UniProt

Q12445 - POM34_YEAST

Protein

Nucleoporin POM34

Gene

POM34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors.

    GO - Molecular functioni

    1. structural constituent of nuclear pore Source: SGD

    GO - Biological processi

    1. mRNA transport Source: UniProtKB-KW
    2. nuclear pore organization Source: SGD
    3. protein import into nucleus Source: SGD
    4. spindle pole body duplication Source: SGD

    Keywords - Biological processi

    mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32179-MONOMER.

    Protein family/group databases

    TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoporin POM34
    Alternative name(s):
    Nuclear pore protein POM34
    Pore membrane protein POM34
    Gene namesi
    Name:POM34
    Ordered Locus Names:YLR018C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR018c.
    SGDiS000004008. POM34.

    Subcellular locationi

    Nucleusnuclear pore complex. Nucleus membrane; Multi-pass membrane protein
    Note: Central core structure of the nuclear pore complex.

    GO - Cellular componenti

    1. integral component of nuclear outer membrane Source: SGD
    2. nuclear pore Source: SGD
    3. nuclear pore transmembrane ring Source: SGD

    Keywords - Cellular componenti

    Membrane, Nuclear pore complex, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 299299Nucleoporin POM34PRO_0000204911Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei270 – 2701Phosphoserine1 Publication
    Modified residuei273 – 2731Phosphothreonine1 Publication
    Modified residuei292 – 2921Phosphoserine1 Publication
    Modified residuei294 – 2941Phosphoserine4 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12445.
    PaxDbiQ12445.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12445.

    Interactioni

    Subunit structurei

    The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof.

    Protein-protein interaction databases

    BioGridi31292. 94 interactions.
    DIPiDIP-8961N.
    IntActiQ12445. 6 interactions.
    MINTiMINT-1362914.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12445.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei133 – 15321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG45366.
    OrthoDBiEOG71VT5V.

    Family and domain databases

    InterProiIPR012578. Nucl_pore_cmplx.
    [Graphical view]
    PfamiPF08058. NPCC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12445-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIQAGQLGL DDNDVPGPLP DTDSKPSSQS QNDTPMFKLG NFESPVLKEL    50
    SRRTVNKEME TQRIMTNVIA FAFWNLLVKF IKFFWNNTHV GRQFCNRLSR 100
    IHLYMLTFHT LKKANIIYHT TFSWLNAELL DYLFHLLISL NILFSLWKLL 150
    STVKVSDLNL TDRQKKLLGV DMQSSVDTGL QPQHPHYVST SKISQMAQNK 200
    THIPQTNLKN HPAYLFKGLE TPLKARQREM AEEQTKLQSQ SLHTKNVFGT 250
    LQRHSGISST LVSANNDNNS PHTPVTRKGY IPSSKYAYMM NSQSPRGKI 299
    Length:299
    Mass (Da):34,232
    Last modified:November 1, 1996 - v1
    Checksum:iEEDB5BEFF5BEDF6A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti191 – 1911S → P in AAS56272. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90564 Genomic DNA. Translation: CAA62155.1.
    Z73191 Genomic DNA. Translation: CAA97542.1.
    Z73190 Genomic DNA. Translation: CAA97540.1.
    AY557946 Genomic DNA. Translation: AAS56272.1.
    BK006945 Genomic DNA. Translation: DAA09336.1.
    PIRiS64840.
    RefSeqiNP_013118.1. NM_001181905.1.

    Genome annotation databases

    EnsemblFungiiYLR018C; YLR018C; YLR018C.
    GeneIDi850705.
    KEGGisce:YLR018C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90564 Genomic DNA. Translation: CAA62155.1 .
    Z73191 Genomic DNA. Translation: CAA97542.1 .
    Z73190 Genomic DNA. Translation: CAA97540.1 .
    AY557946 Genomic DNA. Translation: AAS56272.1 .
    BK006945 Genomic DNA. Translation: DAA09336.1 .
    PIRi S64840.
    RefSeqi NP_013118.1. NM_001181905.1.

    3D structure databases

    ProteinModelPortali Q12445.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31292. 94 interactions.
    DIPi DIP-8961N.
    IntActi Q12445. 6 interactions.
    MINTi MINT-1362914.

    Protein family/group databases

    TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

    Proteomic databases

    MaxQBi Q12445.
    PaxDbi Q12445.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR018C ; YLR018C ; YLR018C .
    GeneIDi 850705.
    KEGGi sce:YLR018C.

    Organism-specific databases

    CYGDi YLR018c.
    SGDi S000004008. POM34.

    Phylogenomic databases

    eggNOGi NOG45366.
    OrthoDBi EOG71VT5V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32179-MONOMER.

    Miscellaneous databases

    NextBioi 966751.

    Gene expression databases

    Genevestigatori Q12445.

    Family and domain databases

    InterProi IPR012578. Nucl_pore_cmplx.
    [Graphical view ]
    Pfami PF08058. NPCC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 7.8kb fragment from chromosome XII of Saccharomyces cerevisiae does not harbour PKC2."
      Saville S.P., Atkinson S., Jamieson L., Pocklington M.J., Orr E.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
      Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
      J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
    6. "Peering through the pore: nuclear pore complex structure, assembly, and function."
      Suntharalingam M., Wente S.R.
      Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; THR-273; SER-292 AND SER-294, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPOM34_YEAST
    AccessioniPrimary (citable) accession number: Q12445
    Secondary accession number(s): D6VY20, E9P8U3, Q7LGY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3