Nucleoporin POM34 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12445 (POM34_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Nucleoporin POM34

Alternative name(s):
Nuclear pore protein POM34
Pore membrane protein POM34

Gene names

Name:	POM34
Ordered Locus Names:	YLR018C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors.
Subunit structure	The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Ref.5
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Multi-pass membrane protein. Note: Central core structure of the nuclear pore complex.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW nuclear pore organization Inferred from genetic interaction PubMed 19414609. Source: SGD protein import into nucleus Inferred from genetic interaction PubMed 16361228. Source: SGD spindle pole body duplication associated with nuclear envelope Inferred from genetic interaction PubMed 19571182 PubMed 20713690. Source: SGD
Cellular_component	integral to nuclear outer membrane Inferred from direct assay PubMed 16361228. Source: SGD nuclear pore transmembrane ring Inferred from direct assay Ref.5 PubMed 19414609. Source: SGD
Molecular_function	structural constituent of nuclear pore Inferred by curator Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 299

299

Nucleoporin POM34

PRO_0000204911

Regions

Transmembrane

64 – 84

Helical; Potential

Transmembrane

133 – 153

Helical; Potential

Amino acid modifications

Modified residue

292

Phosphoserine Ref.6

Modified residue

294

Phosphoserine Ref.6 Ref.8 Ref.9 Ref.10

Experimental info

Sequence conflict

191

S → P in AAS56272. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q12445 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EEDB5BEFF5BEDF6A
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FASTA

299

34,232

        10         20         30         40         50         60 
MKIQAGQLGL DDNDVPGPLP DTDSKPSSQS QNDTPMFKLG NFESPVLKEL SRRTVNKEME 

        70         80         90        100        110        120 
TQRIMTNVIA FAFWNLLVKF IKFFWNNTHV GRQFCNRLSR IHLYMLTFHT LKKANIIYHT 

       130        140        150        160        170        180 
TFSWLNAELL DYLFHLLISL NILFSLWKLL STVKVSDLNL TDRQKKLLGV DMQSSVDTGL 

       190        200        210        220        230        240 
QPQHPHYVST SKISQMAQNK THIPQTNLKN HPAYLFKGLE TPLKARQREM AEEQTKLQSQ 

       250        260        270        280        290 
SLHTKNVFGT LQRHSGISST LVSANNDNNS PHTPVTRKGY IPSSKYAYMM NSQSPRGKI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 7.8kb fragment from chromosome XII of Saccharomyces cerevisiae does not harbour PKC2." Saville S.P., Atkinson S., Jamieson L., Pocklington M.J., Orr E. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[6]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-294, MASS SPECTROMETRY. Strain: 2124.
[7]	"Peering through the pore: nuclear pore complex structure, assembly, and function." Suntharalingam M., Wente S.R. Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X90564 Genomic DNA. Translation: CAA62155.1. Z73191 Genomic DNA. Translation: CAA97542.1. Z73190 Genomic DNA. Translation: CAA97540.1. AY557946 Genomic DNA. Translation: AAS56272.1. BK006945 Genomic DNA. Translation: DAA09336.1.
PIR	S64840.
RefSeq	NP_013118.1. NM_001181905.1.
3D structure databases
ProteinModelPortal	Q12445.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8961N.
IntAct	Q12445. 2 interactions.
MINT	MINT-1362914.
Protein family/group databases
TCDB	9.A.14.1.1. nuclear pore complex (NPC) family.
Proteomic databases
PaxDb	Q12445.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR018C; YLR018C; YLR018C.
GeneID	850705.
KEGG	sce:YLR018C.
Organism-specific databases
CYGD	YLR018c.
SGD	S000004008. POM34.
Phylogenomic databases
eggNOG	NOG45366.
OMA	YIPSSKY.
OrthoDB	EOG41NXWP.
Gene expression databases
Genevestigator	Q12445.
GermOnline	YLR018C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012578. Nucl_pore_cmplx. [Graphical view]
Pfam	PF08058. NPCC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966751.

Entry information

Entry name

POM34_YEAST

Accession

Primary (citable) accession number: Q12445
Secondary accession number(s): D6VY20, E9P8U3, Q7LGY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents