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Protein

Anaphase-promoting complex subunit 2

Gene

APC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.1 Publication

Pathwayi

GO - Biological processi

  1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  2. cell division Source: UniProtKB-KW
  3. exit from mitosis Source: SGD
  4. metaphase/anaphase transition of mitotic cell cycle Source: SGD
  5. negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation Source: SGD
  6. protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32269-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaphase-promoting complex subunit 2
Gene namesi
Name:APC2
Synonyms:RSI1
Ordered Locus Names:YLR127C
ORF Names:L3105, L3108
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

CYGDiYLR127c.
SGDiS000004117. APC2.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. anaphase-promoting complex Source: SGD
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 853853Anaphase-promoting complex subunit 2PRO_0000119814Add
BLAST

Proteomic databases

MaxQBiQ12440.
PaxDbiQ12440.

Expressioni

Gene expression databases

GenevestigatoriQ12440.

Interactioni

Subunit structurei

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC2 interacts directly with APC11 thereby anchoring APC11 to the core complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MND2P405773EBI-33503,EBI-25433

Protein-protein interaction databases

BioGridi31396. 44 interactions.
DIPiDIP-939N.
IntActiQ12440. 15 interactions.
MINTiMINT-619316.

Structurei

Secondary structure

1
853
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi775 – 79218Combined sources
Beta strandi794 – 7963Combined sources
Helixi797 – 80711Combined sources
Helixi810 – 8123Combined sources
Helixi819 – 83113Combined sources
Beta strandi834 – 8363Combined sources
Turni839 – 8413Combined sources
Beta strandi842 – 8454Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LDDX-ray2.00A/B/C/D773-846[»]
ProteinModelPortaliQ12440.
SMRiQ12440. Positions 773-846.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12440.

Family & Domainsi

Sequence similaritiesi

Belongs to the cullin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286372.
GeneTreeiENSGT00390000016127.
HOGENOMiHOG000001122.
InParanoidiQ12440.
KOiK03349.
OMAiNEIFDIC.
OrthoDBiEOG7W15CT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR014786. APC_su2_C.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08672. APC2. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SMARTiSM01013. APC2. 1 hit.
[Graphical view]
SUPFAMiSSF75632. SSF75632. 1 hit.
PROSITEiPS50069. CULLIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12440-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH
60 70 80 90 100
QLRPPSLRIK NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL
110 120 130 140 150
QLFSTLKEFY IFQVRYHFFL HFNNINYLKD IQRWENYYEF PLRYVPIFDV
160 170 180 190 200
NVNDWALELN SLRHYLLNRN IKFKNNLRTR LDKLIMDDDF DLADNLIQWL
210 220 230 240 250
KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM ETFNKFINQY
260 270 280 290 300
WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV
310 320 330 340 350
TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA
360 370 380 390 400
FLVLDPTGRC LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF
410 420 430 440 450
NVDMKALLSL VDTLHDSDIN QDTNITKRDK NKKSPFLWNL KVKGKRELNK
460 470 480 490 500
DLPIRHAMLY EHILNYYIAW VPEPNDMIPG NIKSSYIKTN LFEVLLDLFE
510 520 530 540 550
SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI VKFTETSHSN
560 570 580 590 600
YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL
610 620 630 640 650
DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP
660 670 680 690 700
GRKLQLCKDK GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL
710 720 730 740 750
SLEQLSESLN IAPPRLTHLL DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ
760 770 780 790 800
KTAPMEIENS NYELHNDSEI ERKYELTLQR SLPFIEGMLT NLGAMKLHKI
810 820 830 840 850
HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN GSYEIVKNGH

KNS
Length:853
Mass (Da):99,978
Last modified:October 31, 1996 - v1
Checksum:iE559A0E28C784DE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61705.1.
Z73299 Genomic DNA. Translation: CAA97696.1.
U53877 Genomic DNA. Translation: AAB82373.1.
X91258 Genomic DNA. Translation: CAA62638.1.
Z73300 Genomic DNA. Translation: CAA97698.1.
AY899244 mRNA. Translation: AAX83929.1.
BK006945 Genomic DNA. Translation: DAA09438.1.
PIRiS59315.
RefSeqiNP_013228.1. NM_001182014.1.

Genome annotation databases

EnsemblFungiiYLR127C; YLR127C; YLR127C.
GeneIDi850818.
KEGGisce:YLR127C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61705.1.
Z73299 Genomic DNA. Translation: CAA97696.1.
U53877 Genomic DNA. Translation: AAB82373.1.
X91258 Genomic DNA. Translation: CAA62638.1.
Z73300 Genomic DNA. Translation: CAA97698.1.
AY899244 mRNA. Translation: AAX83929.1.
BK006945 Genomic DNA. Translation: DAA09438.1.
PIRiS59315.
RefSeqiNP_013228.1. NM_001182014.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LDDX-ray2.00A/B/C/D773-846[»]
ProteinModelPortaliQ12440.
SMRiQ12440. Positions 773-846.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31396. 44 interactions.
DIPiDIP-939N.
IntActiQ12440. 15 interactions.
MINTiMINT-619316.

Proteomic databases

MaxQBiQ12440.
PaxDbiQ12440.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR127C; YLR127C; YLR127C.
GeneIDi850818.
KEGGisce:YLR127C.

Organism-specific databases

CYGDiYLR127c.
SGDiS000004117. APC2.

Phylogenomic databases

eggNOGiNOG286372.
GeneTreeiENSGT00390000016127.
HOGENOMiHOG000001122.
InParanoidiQ12440.
KOiK03349.
OMAiNEIFDIC.
OrthoDBiEOG7W15CT.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-32269-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12440.
NextBioi967061.

Gene expression databases

GenevestigatoriQ12440.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR014786. APC_su2_C.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF08672. APC2. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SMARTiSM01013. APC2. 1 hit.
[Graphical view]
SUPFAMiSSF75632. SSF75632. 1 hit.
PROSITEiPS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found."
    Verhasselt P., Volckaert G.
    Yeast 13:241-250(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90840 / EAY235 / FY23.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
    Zhang Z., Dietrich F.S.
    Nucleic Acids Res. 33:2838-2851(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-78.
    Strain: ATCC 208353 / W303-1A.
  5. "Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins."
    Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J., Mann M., Nasmyth K.
    Science 279:1216-1219(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 773-846.

Entry informationi

Entry nameiAPC2_YEAST
AccessioniPrimary (citable) accession number: Q12440
Secondary accession number(s): D6VYC2, Q2VQX4, Q7LGV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2004
Last sequence update: October 31, 1996
Last modified: March 3, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.