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Q12440 (APC2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaphase-promoting complex subunit 2
Gene names
Name:APC2
Synonyms:RSI1
Ordered Locus Names:YLR127C
ORF Names:L3105, L3108
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length853 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. Ref.5

Pathway

Protein modification; protein ubiquitination.

Subunit structure

The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC2 interacts directly with APC11 thereby anchoring APC11 to the core complex. Ref.5

Subcellular location

Cytoplasm. Nucleus Ref.6.

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the cullin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MND2P405773EBI-33503,EBI-25433

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 853853Anaphase-promoting complex subunit 2
PRO_0000119814

Secondary structure

............... 853
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12440 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E559A0E28C784DE2

FASTA85399,978
        10         20         30         40         50         60 
MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH QLRPPSLRIK 

        70         80         90        100        110        120 
NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL QLFSTLKEFY IFQVRYHFFL 

       130        140        150        160        170        180 
HFNNINYLKD IQRWENYYEF PLRYVPIFDV NVNDWALELN SLRHYLLNRN IKFKNNLRTR 

       190        200        210        220        230        240 
LDKLIMDDDF DLADNLIQWL KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM 

       250        260        270        280        290        300 
ETFNKFINQY WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV 

       310        320        330        340        350        360 
TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA FLVLDPTGRC 

       370        380        390        400        410        420 
LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF NVDMKALLSL VDTLHDSDIN 

       430        440        450        460        470        480 
QDTNITKRDK NKKSPFLWNL KVKGKRELNK DLPIRHAMLY EHILNYYIAW VPEPNDMIPG 

       490        500        510        520        530        540 
NIKSSYIKTN LFEVLLDLFE SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI 

       550        560        570        580        590        600 
VKFTETSHSN YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL 

       610        620        630        640        650        660 
DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP GRKLQLCKDK 

       670        680        690        700        710        720 
GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL SLEQLSESLN IAPPRLTHLL 

       730        740        750        760        770        780 
DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ KTAPMEIENS NYELHNDSEI ERKYELTLQR 

       790        800        810        820        830        840 
SLPFIEGMLT NLGAMKLHKI HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN 

       850 
GSYEIVKNGH KNS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found."
Verhasselt P., Volckaert G.
Yeast 13:241-250(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 90840 / EAY235 / FY23.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
Zhang Z., Dietrich F.S.
Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-78.
Strain: ATCC 208353 / W303-1A.
[5]"Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins."
Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J., Mann M., Nasmyth K.
Science 279:1216-1219(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Structure of the Cul1-Rbx1-Skp1-F box Skp2 SCF ubiquitin ligase complex."
Zheng N., Schulman B.A., Song L., Miller J.J., Jeffrey P.D., Wang P., Chu C., Koepp D.M., Elledge S.J., Pagano M., Conaway R.C., Conaway J.W., Harper J.W., Pavletich N.P.
Nature 416:703-709(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 773-846.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89514 Genomic DNA. Translation: CAA61705.1.
Z73299 Genomic DNA. Translation: CAA97696.1.
U53877 Genomic DNA. Translation: AAB82373.1.
X91258 Genomic DNA. Translation: CAA62638.1.
Z73300 Genomic DNA. Translation: CAA97698.1.
AY899244 mRNA. Translation: AAX83929.1.
BK006945 Genomic DNA. Translation: DAA09438.1.
PIRS59315.
RefSeqNP_013228.1. NM_001182014.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1LDDX-ray2.00A/B/C/D773-846[»]
ProteinModelPortalQ12440.
SMRQ12440. Positions 773-846.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31396. 46 interactions.
DIPDIP-939N.
IntActQ12440. 15 interactions.
MINTMINT-619316.

Proteomic databases

PaxDbQ12440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR127C; YLR127C; YLR127C.
GeneID850818.
KEGGsce:YLR127C.

Organism-specific databases

CYGDYLR127c.
SGDS000004117. APC2.

Phylogenomic databases

eggNOGNOG286372.
GeneTreeENSGT00390000016127.
HOGENOMHOG000001122.
KOK03349.
OMANEIFDIC.
OrthoDBEOG7W15CT.

Enzyme and pathway databases

BioCycYEAST:G3O-32269-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ12440.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR014786. APC_su2_C.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF08672. APC2. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SMARTSM01013. APC2. 1 hit.
[Graphical view]
SUPFAMSSF75632. SSF75632. 1 hit.
PROSITEPS50069. CULLIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ12440.
NextBio967061.

Entry information

Entry nameAPC2_YEAST
AccessionPrimary (citable) accession number: Q12440
Secondary accession number(s): D6VYC2, Q2VQX4, Q7LGV7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways