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Q12440

- APC2_YEAST

UniProt

Q12440 - APC2_YEAST

Protein

Anaphase-promoting complex subunit 2

Gene

APC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
    2. cyclin catabolic process Source: SGD
    3. exit from mitosis Source: SGD
    4. metaphase/anaphase transition of mitotic cell cycle Source: SGD
    5. protein ubiquitination Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32269-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaphase-promoting complex subunit 2
    Gene namesi
    Name:APC2
    Synonyms:RSI1
    Ordered Locus Names:YLR127C
    ORF Names:L3105, L3108
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR127c.
    SGDiS000004117. APC2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. anaphase-promoting complex Source: SGD
    2. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 853853Anaphase-promoting complex subunit 2PRO_0000119814Add
    BLAST

    Proteomic databases

    MaxQBiQ12440.
    PaxDbiQ12440.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12440.

    Interactioni

    Subunit structurei

    The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. APC2 interacts directly with APC11 thereby anchoring APC11 to the core complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MND2P405773EBI-33503,EBI-25433

    Protein-protein interaction databases

    BioGridi31396. 46 interactions.
    DIPiDIP-939N.
    IntActiQ12440. 15 interactions.
    MINTiMINT-619316.

    Structurei

    Secondary structure

    1
    853
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi775 – 79218
    Beta strandi794 – 7963
    Helixi797 – 80711
    Helixi810 – 8123
    Helixi819 – 83113
    Beta strandi834 – 8363
    Turni839 – 8413
    Beta strandi842 – 8454

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LDDX-ray2.00A/B/C/D773-846[»]
    ProteinModelPortaliQ12440.
    SMRiQ12440. Positions 773-846.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12440.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cullin family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG286372.
    GeneTreeiENSGT00390000016127.
    HOGENOMiHOG000001122.
    KOiK03349.
    OMAiNEIFDIC.
    OrthoDBiEOG7W15CT.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR014786. APC_su2_C.
    IPR016158. Cullin_homology.
    IPR001373. Cullin_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF08672. APC2. 1 hit.
    PF00888. Cullin. 1 hit.
    [Graphical view]
    SMARTiSM01013. APC2. 1 hit.
    [Graphical view]
    SUPFAMiSSF75632. SSF75632. 1 hit.
    PROSITEiPS50069. CULLIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12440-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFQITPTRD LKVITDELQT LSSYIFHTNI VDDLNSLLTW MSPNDAKSNH    50
    QLRPPSLRIK NIIKVLFPNN ATTSPYSMIN TSQANNSIVN EGNTNKELQL 100
    QLFSTLKEFY IFQVRYHFFL HFNNINYLKD IQRWENYYEF PLRYVPIFDV 150
    NVNDWALELN SLRHYLLNRN IKFKNNLRTR LDKLIMDDDF DLADNLIQWL 200
    KSANGSLSST ELIVNALYSK INKFCEDNMS RVWNKRFMIM ETFNKFINQY 250
    WSQFSKLVGC PEDDHELTTT VFNCFESNFL RIRTNEIFDI CVLAYPDSKV 300
    TLLELRKIMK DFKDYTNIVT TFLSDFKKYI LNPSVTTVDA LLRYVKTIKA 350
    FLVLDPTGRC LHSITTFVKP YFQERKHLVN VLLYAMLDLP EEELKEKINF 400
    NVDMKALLSL VDTLHDSDIN QDTNITKRDK NKKSPFLWNL KVKGKRELNK 450
    DLPIRHAMLY EHILNYYIAW VPEPNDMIPG NIKSSYIKTN LFEVLLDLFE 500
    SREFFISEFR NLLTDRLFTL KFYTLDEKWT RCLKLIREKI VKFTETSHSN 550
    YITNGILGLL ETTAPAADAD QSNLNSIDVM LWDIKCSEEL CRKMHEVAGL 600
    DPIIFPKFIS LLYWKYNCDT QGSNDLAFHL PIDLERELQK YSDIYSQLKP 650
    GRKLQLCKDK GKVEIQLAFK DGRKLVLDVS LEQCSVINQF DSPNDEPICL 700
    SLEQLSESLN IAPPRLTHLL DFWIQKGVLL KENGTYSVIE HSEMDFDQAQ 750
    KTAPMEIENS NYELHNDSEI ERKYELTLQR SLPFIEGMLT NLGAMKLHKI 800
    HSFLKITVPK DWGYNRITLQ QLEGYLNTLA DEGRLKYIAN GSYEIVKNGH 850
    KNS 853
    Length:853
    Mass (Da):99,978
    Last modified:November 1, 1996 - v1
    Checksum:iE559A0E28C784DE2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89514 Genomic DNA. Translation: CAA61705.1.
    Z73299 Genomic DNA. Translation: CAA97696.1.
    U53877 Genomic DNA. Translation: AAB82373.1.
    X91258 Genomic DNA. Translation: CAA62638.1.
    Z73300 Genomic DNA. Translation: CAA97698.1.
    AY899244 mRNA. Translation: AAX83929.1.
    BK006945 Genomic DNA. Translation: DAA09438.1.
    PIRiS59315.
    RefSeqiNP_013228.1. NM_001182014.1.

    Genome annotation databases

    EnsemblFungiiYLR127C; YLR127C; YLR127C.
    GeneIDi850818.
    KEGGisce:YLR127C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89514 Genomic DNA. Translation: CAA61705.1 .
    Z73299 Genomic DNA. Translation: CAA97696.1 .
    U53877 Genomic DNA. Translation: AAB82373.1 .
    X91258 Genomic DNA. Translation: CAA62638.1 .
    Z73300 Genomic DNA. Translation: CAA97698.1 .
    AY899244 mRNA. Translation: AAX83929.1 .
    BK006945 Genomic DNA. Translation: DAA09438.1 .
    PIRi S59315.
    RefSeqi NP_013228.1. NM_001182014.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LDD X-ray 2.00 A/B/C/D 773-846 [» ]
    ProteinModelPortali Q12440.
    SMRi Q12440. Positions 773-846.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31396. 46 interactions.
    DIPi DIP-939N.
    IntActi Q12440. 15 interactions.
    MINTi MINT-619316.

    Proteomic databases

    MaxQBi Q12440.
    PaxDbi Q12440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR127C ; YLR127C ; YLR127C .
    GeneIDi 850818.
    KEGGi sce:YLR127C.

    Organism-specific databases

    CYGDi YLR127c.
    SGDi S000004117. APC2.

    Phylogenomic databases

    eggNOGi NOG286372.
    GeneTreei ENSGT00390000016127.
    HOGENOMi HOG000001122.
    KOi K03349.
    OMAi NEIFDIC.
    OrthoDBi EOG7W15CT.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci YEAST:G3O-32269-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12440.
    NextBioi 967061.

    Gene expression databases

    Genevestigatori Q12440.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR014786. APC_su2_C.
    IPR016158. Cullin_homology.
    IPR001373. Cullin_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF08672. APC2. 1 hit.
    PF00888. Cullin. 1 hit.
    [Graphical view ]
    SMARTi SM01013. APC2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75632. SSF75632. 1 hit.
    PROSITEi PS50069. CULLIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found."
      Verhasselt P., Volckaert G.
      Yeast 13:241-250(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 90840 / EAY235 / FY23.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
      Zhang Z., Dietrich F.S.
      Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-78.
      Strain: ATCC 208353 / W303-1A.
    5. "Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins."
      Zachariae W., Shevchenko A., Andrews P.D., Ciosk R., Galova M., Stark M.J., Mann M., Nasmyth K.
      Science 279:1216-1219(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBUNIT.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 773-846.

    Entry informationi

    Entry nameiAPC2_YEAST
    AccessioniPrimary (citable) accession number: Q12440
    Secondary accession number(s): D6VYC2, Q2VQX4, Q7LGV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 23, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 432 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3