Q12433 (AHC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein AHC1

Alternative name(s):
ADA HAT complex component 1

Gene names

Name:	AHC1
Ordered Locus Names:	YOR023C
ORF Names:	OR26.13

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as component of the transcription regulatory histone acetylation (HAT) complex ADA. ADA preferentially acetylates nucleosomal histones H3 (at 'Lys-14' and 'Lys-18') and H2B. AHC1 is required for the overall structural integrity of the ADA complex. Ref.3 Ref.5 Ref.6
Subunit structure	Component of the 0.8 MDa ADA complex, which at least consists of ADA2, ADA3, AHC1 and GCN5.
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 339 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histone acetylation Inferred from direct assay Ref.4. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Ada2/Gcn5/Ada3 transcription activator complex Inferred from physical interaction Ref.4. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AHC2	P25649	6	EBI-33947,EBI-21993

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Protein AHC1		PRO_0000227804
Amino acid modifications
	Modified residue	37	1	Phosphoserine Ref.10
	Modified residue	505	1	Phosphoserine Ref.9 Ref.10
	Modified residue	510	1	Phosphothreonine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q12433 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C18AFA10E4334764	FASTA	566	63,869
	10 20 30 40 50 60 MMSPAQDKLQ HQHHNPNSSS SSSSKMTNVY QVTTPKSPQD LENNMDEPFK MDTATSNPDK 70 80 90 100 110 120 DSENTQRLKY ECAKGEIQNV LNLHIMLNHK HVRHLRRNVQ KVNAKLALLE TLHKDTGLLN 130 140 150 160 170 180 KIERTYQLKI KQHQQHSVLG GHFHDSTATE NTNASNYNLS YPVLSDYNIN CQPLSSSSNR 190 200 210 220 230 240 NLSTTRIPHH HYHTRSKSNG LLLEPSALRP ANSNIIDYRL TGSKSLSEAI TKPTPVSLPH 250 260 270 280 290 300 SNSDGISSPR SSSISPLDEQ PGFQILPFKP SQMHLNHRRN YSSTCLTSNS GIIGKTENNE 310 320 330 340 350 360 PIFRRYDGIL VIITCSKCDR SGFTSAQGIV NHTRLKHSKL YSSQPLAVLN NQKLLPNDKQ 370 380 390 400 410 420 DPEILSKFKK LNLDPNKDYL PSDIAIPKPQ SPINHSENHT RAPKTVKNTP HLEKLYQNKE 430 440 450 460 470 480 DFKKLIDMVN ETPDDLNEYL KQREIQLRYQ KEQEEESSKS DDEASYVPSP SLSATATTTT 490 500 510 520 530 540 TTDPPSPPVL SSSLQRKLLR KRKLSLNSST PMEDLPLRER LRANPTDKKP RKAALLTNEL 550 560 EGPDPAAKSS SYYNLRSKSR LRGSHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex." Grant P.A., Duggan L., Cote J., Roberts S.M., Brownell J.E., Candau R., Ohba R., Owen-Hughes T., Allis C.D., Winston F., Berger S.L., Workman J.L. Genes Dev. 11:1640-1650(1997) [PubMed: 9224714] [Abstract] Cited for: FUNCTION OF THE ADA COMPLEX.
[4]	"The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae." Eberharter A., Sterner D.E., Schieltz D., Hassan A., Yates J.R. III, Berger S.L., Workman J.L. Mol. Cell. Biol. 19:6621-6631(1999) [PubMed: 10490601] [Abstract] Cited for: IDENTIFICATION IN THE ADA COMPLEX, MASS SPECTROMETRY.
[5]	"Expanded lysine acetylation specificity of Gcn5 in native complexes." Grant P.A., Eberharter A., John S., Cook R.G., Turner B.M., Workman J.L. J. Biol. Chem. 274:5895-5900(1999) [PubMed: 10026213] [Abstract] Cited for: FUNCTION OF THE ADA COMPLEX.
[6]	"A Gal4-sigma 54 hybrid protein that functions as a potent activator of RNA polymerase II transcription in yeast." Chen B.-S., Sun Z.-W., Hampsey M. J. Biol. Chem. 276:23881-23887(2001) [PubMed: 11313364] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-505, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37; SER-505 AND THR-510, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74931 Genomic DNA. Translation: CAA99213.1. X87331 Genomic DNA. Translation: CAA60772.1. BK006948 Genomic DNA. Translation: DAA10806.1.
PIR	S54629.
RefSeq	NP_014666.1. NM_001183442.1.
3D structure databases
ProteinModelPortal	Q12433.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-896N.
IntAct	Q12433. 10 interactions.
MINT	MINT-391720.
STRING	Q12433.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR023C; YOR023C; YOR023C.
GeneID	854188.
KEGG	sce:YOR023C.
NMPDR	fig|4932.3.peg.5764.
Organism-specific databases
CYGD	YOR023c.
SGD	S000005549. AHC1.
Phylogenomic databases
eggNOG	fuNOG09070.
HOGENOM	HBG202804.
OMA	AQGIVNH.
OrthoDB	EOG4WWVTV.
Gene expression databases
ArrayExpress	Q12433.
Genevestigator	Q12433.
GermOnline	YOR023C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015880. Znf_C2H2-like. [Graphical view]
KO	K11370.
SMART	SM00355. ZnF_C2H2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976005.

Entry information

Entry name

AHC1_YEAST

Accession

Primary (citable) accession number: Q12433
Secondary accession number(s): D6W290

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program