Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chromatin modification-related protein EAF3

Gene

EAF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair.3 Publications

Miscellaneous

Present with 1890 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • chromatin remodeling Source: GO_Central
  • chromatin silencing Source: GO_Central
  • DNA repair Source: SGD
  • histone acetylation Source: SGD
  • histone deacetylation Source: SGD
  • histone H2A acetylation Source: GO_Central
  • histone H4 acetylation Source: GO_Central
  • negative regulation of antisense RNA transcription Source: SGD
  • negative regulation of transcription, DNA-templated Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
  • regulation of transcription by RNA polymerase II Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionChromatin regulator
Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34183-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin modification-related protein EAF3
Alternative name(s):
ESA1-associated factor 3
Gene namesi
Name:EAF3
Ordered Locus Names:YPR023C
ORF Names:YP9367.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR023C
SGDiS000006227 EAF3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000887831 – 401Chromatin modification-related protein EAF3Add BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12432
PaxDbiQ12432
PRIDEiQ12432

PTM databases

iPTMnetiQ12432

Interactioni

Subunit structurei

Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, YAF9, VID21, SWC4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, TRA1 and YNG2.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36200, 394 interactors
DIPiDIP-2871N
IntActiQ12432, 40 interactors
MINTiQ12432
STRINGi4932.YPR023C

Structurei

Secondary structure

1401
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 6Combined sources4
Beta strandi13 – 18Combined sources6
Beta strandi21 – 32Combined sources12
Turni33 – 36Combined sources4
Beta strandi37 – 40Combined sources4
Beta strandi47 – 50Combined sources4
Beta strandi54 – 56Combined sources3
Turni69 – 73Combined sources5
Beta strandi76 – 81Combined sources6
Helixi86 – 88Combined sources3
Beta strandi90 – 92Combined sources3
Turni94 – 96Combined sources3
Beta strandi97 – 100Combined sources4
Helixi102 – 113Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2K3XNMR-A1-113[»]
2K3YNMR-A1-115[»]
3E9FX-ray1.80A1-113[»]
3E9GX-ray2.50A/B1-124[»]
ProteinModelPortaliQ12432
SMRiQ12432
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12432

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini216 – 399MRGPROSITE-ProRule annotationAdd BLAST184

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi148 – 205Ser-richAdd BLAST58

Sequence similaritiesi

Belongs to the MRG family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063018
HOGENOMiHOG000190863
InParanoidiQ12432
KOiK11339
OMAiPHYFVHY
OrthoDBiEOG092C4JE4

Family and domain databases

CDDicd00024 CHROMO, 1 hit
Gene3Di1.10.274.30, 1 hit
InterProiView protein in InterPro
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR008676 MRG
IPR038217 MRG_C_sf
IPR026541 MRG_dom
IPR025995 Tudor-knot
PANTHERiPTHR10880 PTHR10880, 1 hit
PfamiView protein in Pfam
PF05712 MRG, 1 hit
PF11717 Tudor-knot, 1 hit
SMARTiView protein in SMART
SM00298 CHROMO, 1 hit
SUPFAMiSSF54160 SSF54160, 3 hits
PROSITEiView protein in PROSITE
PS51640 MRG, 1 hit

Sequencei

Sequence statusi: Complete.

Q12432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDLEQEFAL GGRCLAFHGP LMYEAKILKI WDPSSKMYTS IPNDKPGGSS
60 70 80 90 100
QATKEIKPQK LGEDESIPEE IINGKCFFIH YQGWKSSWDE WVGYDRIRAY
110 120 130 140 150
NEENIAMKKR LANEAKEAKK SLLEQQKKKK LSTSLGGPSN GGKRKGDSRS
160 170 180 190 200
NASISKSTSQ SFLTSSVSGR KSGRSSANSL HPGSSLRSSS DQNGNDDRRR
210 220 230 240 250
SSSLSPNMLH HIAGYPTPKI SLQIPIKLKS VLVDDWEYVT KDKKICRLPA
260 270 280 290 300
DVTVEMVLNK YEHEVSQELE SPGSQSQLSE YCAGLKLYFD KCLGNMLLYR
310 320 330 340 350
LERLQYDELL KKSSKDQKPL VPIRIYGAIH LLRLISVLPE LISSTTMDLQ
360 370 380 390 400
SCQLLIKQTE DFLVWLLMHV DEYFNDKDPN RSDDALYVNT SSQYEGVALG

M
Length:401
Mass (Da):45,203
Last modified:November 1, 1996 - v1
Checksum:i63758DE9510D70DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA Translation: CAA95019.1
Z49274 Genomic DNA Translation: CAA89277.1
BK006949 Genomic DNA Translation: DAA11449.1
PIRiS54497
RefSeqiNP_015348.1, NM_001184120.1

Genome annotation databases

EnsemblFungiiYPR023C; YPR023C; YPR023C
GeneIDi856134
KEGGisce:YPR023C

Similar proteinsi

Entry informationi

Entry nameiEAF3_YEAST
AccessioniPrimary (citable) accession number: Q12432
Secondary accession number(s): D6W433
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health