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Q12428 (PRPD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 2-methylcitrate dehydratase

EC=4.2.1.79
Gene names
Name:PDH1
Ordered Locus Names:YPR002W
ORF Names:LPZ2W, YP9723.02
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length516 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.

Catalytic activity

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

Cofactor

Binds 1 2Fe-2S cluster By similarity.

Pathway

Organic acid metabolism; propanoate degradation.

Miscellaneous

Present with 2730 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PrpD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 516516Probable 2-methylcitrate dehydratase
PRO_0000215027

Sequences

Sequence LengthMass (Da)Tools
Q12428 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2ED7F1AF648F2AAC

FASTA51657,684
        10         20         30         40         50         60 
MFLAKNLKNN KIKVCLPKKK FAALSTASIQ TNERPNPDKV LKDIAKYVHE TPLKSSLALD 

        70         80         90        100        110        120 
TARLCFLDTL GCGLAALKFK QAQNIIKPIV PGTIVPSGTK ILGTSYVMDP VKGAFAIGTL 

       130        140        150        160        170        180 
IRWLDYNDCW LAAEWGHPSD NLGGILAVAD HLSRLNKATH GKNGKQFLVK DVLEAMIKAH 

       190        200        210        220        230        240 
EIQGIIALEN SFNKVGLDHV VLVKVATAGV VSKMLGLSQE QTIEALSQAF VDGQSLRTYR 

       250        260        270        280        290        300 
HAPNTGSRKS WAAGDAVSRA VNLAYLVKNA NVGTIPSVLT ARTWGFYDVL FKGKPFSFQQ 

       310        320        330        340        350        360 
RSKYDSYVME NVLFKISFPA EFHAQTAVEA AVKAYRILAK QGKTFKDIKS IRIRTQEAAM 

       370        380        390        400        410        420 
RIIDKSGPLY NYADRDHCIQ YMIAVPLITG NLTATDYSDE VARNPEIDNL RSKMYCIEDT 

       430        440        450        460        470        480 
HLTQNYHDPD KRSIGNALLI ELNDGTQLDE IFVEYPVGHK FRREEGIPLL MNKFQRHLRE 

       490        500        510 
HFVESPDKVD LIMKVSSKTN FLNMQIDKYM DLFTEG 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71255 Genomic DNA. Translation: CAA95042.1.
Z48951 Genomic DNA. Translation: CAA88780.1.
U31900 Genomic DNA. Translation: AAA97581.1.
BK006949 Genomic DNA. Translation: DAA11429.1.
PIRS52815.
RefSeqNP_015326.1. NM_001184099.1.

3D structure databases

ProteinModelPortalQ12428.
SMRQ12428. Positions 38-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36178. 14 interactions.
IntActQ12428. 1 interaction.
MINTMINT-2784533.
STRING4932.YPR002W.

Proteomic databases

MaxQBQ12428.
PaxDbQ12428.
PeptideAtlasQ12428.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR002W; YPR002W; YPR002W.
GeneID856108.
KEGGsce:YPR002W.

Organism-specific databases

CYGDYPR002w.
SGDS000006206. PDH1.

Phylogenomic databases

eggNOGCOG2079.
HOGENOMHOG000159916.
KOK01720.
OMAFRREEGI.
OrthoDBEOG773XR2.

Enzyme and pathway databases

BioCycYEAST:G3O-34164-MONOMER.
UniPathwayUPA00946.

Gene expression databases

GenevestigatorQ12428.

Family and domain databases

InterProIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERPTHR16943. PTHR16943. 1 hit.
PfamPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMSSF103378. SSF103378. 1 hit.
TIGRFAMsTIGR02330. prpD. 1 hit.
ProtoNetSearch...

Other

NextBio981170.
PROQ12428.

Entry information

Entry namePRPD_YEAST
AccessionPrimary (citable) accession number: Q12428
Secondary accession number(s): D6W413
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways