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Q12428

- PRPD_YEAST

UniProt

Q12428 - PRPD_YEAST

Protein

Probable 2-methylcitrate dehydratase

Gene

PDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate.By similarity

    Catalytic activityi

    (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

    Cofactori

    Binds 1 2Fe-2S cluster.By similarity

    Pathwayi

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 2-methylcitrate dehydratase activity Source: SGD
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. propionate catabolic process, 2-methylcitrate cycle Source: SGD
    2. propionate metabolic process Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34164-MONOMER.
    UniPathwayiUPA00946.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 2-methylcitrate dehydratase (EC:4.2.1.79)
    Gene namesi
    Name:PDH1
    Ordered Locus Names:YPR002W
    ORF Names:LPZ2W, YP9723.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR002w.
    SGDiS000006206. PDH1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 516516Probable 2-methylcitrate dehydratasePRO_0000215027Add
    BLAST

    Proteomic databases

    MaxQBiQ12428.
    PaxDbiQ12428.
    PeptideAtlasiQ12428.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12428.

    Interactioni

    Protein-protein interaction databases

    BioGridi36178. 14 interactions.
    IntActiQ12428. 1 interaction.
    MINTiMINT-2784533.
    STRINGi4932.YPR002W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12428.
    SMRiQ12428. Positions 38-513.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PrpD family.Curated

    Phylogenomic databases

    eggNOGiCOG2079.
    HOGENOMiHOG000159916.
    KOiK01720.
    OMAiFRREEGI.
    OrthoDBiEOG773XR2.

    Family and domain databases

    InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
    IPR005656. MmgE_PrpD.
    [Graphical view]
    PANTHERiPTHR16943. PTHR16943. 1 hit.
    PfamiPF03972. MmgE_PrpD. 1 hit.
    [Graphical view]
    SUPFAMiSSF103378. SSF103378. 1 hit.
    TIGRFAMsiTIGR02330. prpD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q12428-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLAKNLKNN KIKVCLPKKK FAALSTASIQ TNERPNPDKV LKDIAKYVHE    50
    TPLKSSLALD TARLCFLDTL GCGLAALKFK QAQNIIKPIV PGTIVPSGTK 100
    ILGTSYVMDP VKGAFAIGTL IRWLDYNDCW LAAEWGHPSD NLGGILAVAD 150
    HLSRLNKATH GKNGKQFLVK DVLEAMIKAH EIQGIIALEN SFNKVGLDHV 200
    VLVKVATAGV VSKMLGLSQE QTIEALSQAF VDGQSLRTYR HAPNTGSRKS 250
    WAAGDAVSRA VNLAYLVKNA NVGTIPSVLT ARTWGFYDVL FKGKPFSFQQ 300
    RSKYDSYVME NVLFKISFPA EFHAQTAVEA AVKAYRILAK QGKTFKDIKS 350
    IRIRTQEAAM RIIDKSGPLY NYADRDHCIQ YMIAVPLITG NLTATDYSDE 400
    VARNPEIDNL RSKMYCIEDT HLTQNYHDPD KRSIGNALLI ELNDGTQLDE 450
    IFVEYPVGHK FRREEGIPLL MNKFQRHLRE HFVESPDKVD LIMKVSSKTN 500
    FLNMQIDKYM DLFTEG 516
    Length:516
    Mass (Da):57,684
    Last modified:November 1, 1996 - v1
    Checksum:i2ED7F1AF648F2AAC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71255 Genomic DNA. Translation: CAA95042.1.
    Z48951 Genomic DNA. Translation: CAA88780.1.
    U31900 Genomic DNA. Translation: AAA97581.1.
    BK006949 Genomic DNA. Translation: DAA11429.1.
    PIRiS52815.
    RefSeqiNP_015326.1. NM_001184099.1.

    Genome annotation databases

    EnsemblFungiiYPR002W; YPR002W; YPR002W.
    GeneIDi856108.
    KEGGisce:YPR002W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71255 Genomic DNA. Translation: CAA95042.1 .
    Z48951 Genomic DNA. Translation: CAA88780.1 .
    U31900 Genomic DNA. Translation: AAA97581.1 .
    BK006949 Genomic DNA. Translation: DAA11429.1 .
    PIRi S52815.
    RefSeqi NP_015326.1. NM_001184099.1.

    3D structure databases

    ProteinModelPortali Q12428.
    SMRi Q12428. Positions 38-513.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36178. 14 interactions.
    IntActi Q12428. 1 interaction.
    MINTi MINT-2784533.
    STRINGi 4932.YPR002W.

    Proteomic databases

    MaxQBi Q12428.
    PaxDbi Q12428.
    PeptideAtlasi Q12428.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR002W ; YPR002W ; YPR002W .
    GeneIDi 856108.
    KEGGi sce:YPR002W.

    Organism-specific databases

    CYGDi YPR002w.
    SGDi S000006206. PDH1.

    Phylogenomic databases

    eggNOGi COG2079.
    HOGENOMi HOG000159916.
    KOi K01720.
    OMAi FRREEGI.
    OrthoDBi EOG773XR2.

    Enzyme and pathway databases

    UniPathwayi UPA00946 .
    BioCyci YEAST:G3O-34164-MONOMER.

    Miscellaneous databases

    NextBioi 981170.
    PROi Q12428.

    Gene expression databases

    Genevestigatori Q12428.

    Family and domain databases

    InterProi IPR012705. 2Me_IsoCit_deHydtase_PrpD.
    IPR005656. MmgE_PrpD.
    [Graphical view ]
    PANTHERi PTHR16943. PTHR16943. 1 hit.
    Pfami PF03972. MmgE_PrpD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103378. SSF103378. 1 hit.
    TIGRFAMsi TIGR02330. prpD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPRPD_YEAST
    AccessioniPrimary (citable) accession number: Q12428
    Secondary accession number(s): D6W413
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2730 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3