Reviewed, UniProtKB/Swiss-Prot Q12428 (PRPD_YEAST)

Names and origin

Protein names

Recommended name:
    Probable 2-methylcitrate dehydratase
    EC=4.2.1.79

Gene names

Name:	PDH1
Ordered Locus Names:	YPR002W
ORF Names:	YP9723.02, LPZ2W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.
Catalytic activity	(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.
Cofactor	Binds 1 2Fe-2S cluster By similarity.
Pathway	Organic acid metabolism; propanoate degradation.
Miscellaneous	Present with 2730 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the prpD family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 2-methylcitrate dehydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Probable 2-methylcitrate dehydratase		PRO_0000215027

Sequences

	Sequence		Length	Mass (Da)
	Q12428-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2ED7F1AF648F2AAC	FASTA	516	57,684
	10 20 30 40 50 60 MFLAKNLKNN KIKVCLPKKK FAALSTASIQ TNERPNPDKV LKDIAKYVHE TPLKSSLALD 70 80 90 100 110 120 TARLCFLDTL GCGLAALKFK QAQNIIKPIV PGTIVPSGTK ILGTSYVMDP VKGAFAIGTL 130 140 150 160 170 180 IRWLDYNDCW LAAEWGHPSD NLGGILAVAD HLSRLNKATH GKNGKQFLVK DVLEAMIKAH 190 200 210 220 230 240 EIQGIIALEN SFNKVGLDHV VLVKVATAGV VSKMLGLSQE QTIEALSQAF VDGQSLRTYR 250 260 270 280 290 300 HAPNTGSRKS WAAGDAVSRA VNLAYLVKNA NVGTIPSVLT ARTWGFYDVL FKGKPFSFQQ 310 320 330 340 350 360 RSKYDSYVME NVLFKISFPA EFHAQTAVEA AVKAYRILAK QGKTFKDIKS IRIRTQEAAM 370 380 390 400 410 420 RIIDKSGPLY NYADRDHCIQ YMIAVPLITG NLTATDYSDE VARNPEIDNL RSKMYCIEDT 430 440 450 460 470 480 HLTQNYHDPD KRSIGNALLI ELNDGTQLDE IFVEYPVGHK FRREEGIPLL MNKFQRHLRE 490 500 510 HFVESPDKVD LIMKVSSKTN FLNMQIDKYM DLFTEG

References

[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71255 Genomic DNA. Translation: CAA95042.1. Z48951 Genomic DNA. Translation: CAA88780.1. U31900 Genomic DNA. Translation: AAA97581.1.
PIR	S52815.
RefSeq	NP_015326.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q12428. 1 interaction.
STRING	Q12428.
Proteomic databases
PeptideAtlas	Q12428.
Genome annotation databases
Ensembl	YPR002W; YPR002W; YPR002W; Saccharomyces cerevisiae. [Genome view]
GeneID	856108.
GenomeReviews	Gene locus YPR002W in contig U00094_GR.
KEGG	sce:YPR002W.
NMPDR	fig|4932.3.peg.6460.
Organism-specific databases
CYGD	YPR002w.
SGD	S000006206. PDH1.
Phylogenomic databases
HOGENOM	Q12428.
OMA	ALRFPEC.
Enzyme and pathway databases
BRENDA	4.2.1.79. 250.
Gene expression databases
ArrayExpress	Q12428.
Genevestigator	Q12428.
GermOnline	YPR002W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012705. 2Me_IsoCit_deHydtase_PrpD. IPR005656. MmgE_PrpD. [Graphical view]
PANTHER	PTHR16943. MmgE_PrpD. 1 hit.
Pfam	PF03972. MmgE_PrpD. 1 hit. [Graphical view]
TIGRFAMs	TIGR02330. prpD. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	981170.

Entry information

Entry name

PRPD_YEAST

Accession

Primary (citable) accession number: Q12428

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)