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Protein

Iron-sulfur assembly protein 2

Gene

ISA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi89 – 891IronBy similarity
Metal bindingi175 – 1751IronBy similarity
Metal bindingi177 – 1771IronBy similarity

GO - Molecular functioni

  • iron ion binding Source: SGD

GO - Biological processi

  • biotin biosynthetic process Source: SGD
  • iron-sulfur cluster assembly Source: SGD
Complete GO annotation...

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34215-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur assembly protein 2
Gene namesi
Name:ISA2
Ordered Locus Names:YPR067W
ORF Names:YP9499.22
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR067W.
SGDiS000006271. ISA2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Iron-sulfur assembly protein 2PRO_0000077035Add
BLAST

Proteomic databases

MaxQBiQ12425.
PeptideAtlasiQ12425.

Interactioni

Protein-protein interaction databases

BioGridi36239. 17 interactions.
DIPiDIP-6434N.
MINTiMINT-2780101.

Structurei

3D structure databases

ProteinModelPortaliQ12425.
SMRiQ12425. Positions 59-183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005700.
HOGENOMiHOG000228313.
InParanoidiQ12425.
OMAiYTSTACG.
OrthoDBiEOG7QK0R9.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR017870. FeS_cluster_insertion_CS.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAKLLFTRL NFRRPSTTLR QFPLTCFLFH SKAFYSDLVT KEPLITPKRI
60 70 80 90 100
INKTPGLNLS ISERASNRLA EIYRNSKENL RISVESGGCH GFQYNLTLEP
110 120 130 140 150
ATKPDIKNDV KDKEFSDDLD DDDSKDIIYV LPEDKGRVII DSKSLNILNN
160 170 180
TTLTYTNELI GSSFKIINGS LKSSCGCGSS FDIEN
Length:185
Mass (Da):20,877
Last modified:November 1, 1996 - v1
Checksum:iA4719A9EC54EECF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94975.1.
Z49219 Genomic DNA. Translation: CAA89184.1.
BK006949 Genomic DNA. Translation: DAA11487.1.
PIRiS54088.
RefSeqiNP_015392.1. NM_001184164.1.

Genome annotation databases

EnsemblFungiiYPR067W; YPR067W; YPR067W.
GeneIDi856180.
KEGGisce:YPR067W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94975.1.
Z49219 Genomic DNA. Translation: CAA89184.1.
BK006949 Genomic DNA. Translation: DAA11487.1.
PIRiS54088.
RefSeqiNP_015392.1. NM_001184164.1.

3D structure databases

ProteinModelPortaliQ12425.
SMRiQ12425. Positions 59-183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36239. 17 interactions.
DIPiDIP-6434N.
MINTiMINT-2780101.

Proteomic databases

MaxQBiQ12425.
PeptideAtlasiQ12425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR067W; YPR067W; YPR067W.
GeneIDi856180.
KEGGisce:YPR067W.

Organism-specific databases

EuPathDBiFungiDB:YPR067W.
SGDiS000006271. ISA2.

Phylogenomic databases

GeneTreeiENSGT00390000005700.
HOGENOMiHOG000228313.
InParanoidiQ12425.
OMAiYTSTACG.
OrthoDBiEOG7QK0R9.

Enzyme and pathway databases

BioCyciYEAST:G3O-34215-MONOMER.

Miscellaneous databases

NextBioi981349.
PROiQ12425.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR017870. FeS_cluster_insertion_CS.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Mitochondrial Isa2p plays a crucial role in the maturation of cellular iron-sulfur proteins."
    Pelzer W., Muhlenhoff U., Diekert K., Siegmund K., Kispal G., Lill R.
    FEBS Lett. 476:134-139(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiISA2_YEAST
AccessioniPrimary (citable) accession number: Q12425
Secondary accession number(s): D6W471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1560 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.