Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Iron-sulfur assembly protein 2

Gene

ISA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi89IronBy similarity1
Metal bindingi175IronBy similarity1
Metal bindingi177IronBy similarity1

GO - Molecular functioni

GO - Biological processi

  • biotin biosynthetic process Source: SGD
  • iron-sulfur cluster assembly Source: SGD
  • protein maturation Source: GO_Central
  • protein maturation by iron-sulfur cluster transfer Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34215-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur assembly protein 2
Gene namesi
Name:ISA2
Ordered Locus Names:YPR067W
ORF Names:YP9499.22
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR067W.
SGDiS000006271. ISA2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000770351 – 185Iron-sulfur assembly protein 2Add BLAST185

Proteomic databases

MaxQBiQ12425.
PRIDEiQ12425.

Interactioni

Protein-protein interaction databases

BioGridi36239. 17 interactors.
DIPiDIP-6434N.
MINTiMINT-2780101.

Structurei

3D structure databases

ProteinModelPortaliQ12425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HesB/IscA family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005700.
HOGENOMiHOG000228313.
InParanoidiQ12425.
OMAiYTSTACG.
OrthoDBiEOG092C5QRY.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR017870. FeS_cluster_insertion_CS.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAKLLFTRL NFRRPSTTLR QFPLTCFLFH SKAFYSDLVT KEPLITPKRI
60 70 80 90 100
INKTPGLNLS ISERASNRLA EIYRNSKENL RISVESGGCH GFQYNLTLEP
110 120 130 140 150
ATKPDIKNDV KDKEFSDDLD DDDSKDIIYV LPEDKGRVII DSKSLNILNN
160 170 180
TTLTYTNELI GSSFKIINGS LKSSCGCGSS FDIEN
Length:185
Mass (Da):20,877
Last modified:November 1, 1996 - v1
Checksum:iA4719A9EC54EECF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94975.1.
Z49219 Genomic DNA. Translation: CAA89184.1.
BK006949 Genomic DNA. Translation: DAA11487.1.
PIRiS54088.
RefSeqiNP_015392.1. NM_001184164.1.

Genome annotation databases

EnsemblFungiiYPR067W; YPR067W; YPR067W.
GeneIDi856180.
KEGGisce:YPR067W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94975.1.
Z49219 Genomic DNA. Translation: CAA89184.1.
BK006949 Genomic DNA. Translation: DAA11487.1.
PIRiS54088.
RefSeqiNP_015392.1. NM_001184164.1.

3D structure databases

ProteinModelPortaliQ12425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36239. 17 interactors.
DIPiDIP-6434N.
MINTiMINT-2780101.

Proteomic databases

MaxQBiQ12425.
PRIDEiQ12425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR067W; YPR067W; YPR067W.
GeneIDi856180.
KEGGisce:YPR067W.

Organism-specific databases

EuPathDBiFungiDB:YPR067W.
SGDiS000006271. ISA2.

Phylogenomic databases

GeneTreeiENSGT00390000005700.
HOGENOMiHOG000228313.
InParanoidiQ12425.
OMAiYTSTACG.
OrthoDBiEOG092C5QRY.

Enzyme and pathway databases

BioCyciYEAST:G3O-34215-MONOMER.

Miscellaneous databases

PROiQ12425.

Family and domain databases

Gene3Di2.60.300.12. 1 hit.
InterProiIPR000361. FeS_biogenesis.
IPR017870. FeS_cluster_insertion_CS.
[Graphical view]
PfamiPF01521. Fe-S_biosyn. 1 hit.
[Graphical view]
SUPFAMiSSF89360. SSF89360. 1 hit.
PROSITEiPS01152. HESB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISA2_YEAST
AccessioniPrimary (citable) accession number: Q12425
Secondary accession number(s): D6W471
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1560 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.